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Characterization of Streptomyces spp. Producing Indole-3-acetic acid as Biostimulant Agent de Fretes, Charlie Ester; Sembiring, Langkah; Purwestri, Yekti Asih
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.427 KB)

Abstract

Twenty six isolates of Streptomyces spp. obtained from Cyperus rotundus L. rhizosphere were tested forability to produce indole-3-acetic acid (IAA) in yeast malt extract (YM) medium containing 2 mg/mL tryptophan.Screening of the isolates for ability to produce IAA was carried out by adding Salkowski reagent in bacteriaculture and was measured quantitatively by spectrophotometer at λ 530 nm. Thin Layer Chromatography (TLC)method was used to determine IAA. To ensure the IAA production in Streptomyces isolates, gene involved inIAA biosynthesis was detected by amplifying Tryptophan Monooxigenase (iaaM) gene. The study of the effectof tryptophan on the production of IAA was measured at different concentrations of tryptophan (0, 1, 2, 3,4, 5 mg/mL) in the bacterial culture. The result showed that there were two Streptomyces spp. isolates whichcould produce IAA, namely the isolates of Streptomyces sp. MS1 (125.48 μg/mL) and Streptomyces sp. BR27(104.13 μg/mL). The TLC result showed that the compound in both isolates was identifi ed to be IAA. Theamplifi cation results showed that iaaM gene was detected in both isolates. This results indicated that the IAMpathway is predicted involved in the biosynthesis of IAA in the selected isolates. Both of the isolates were ableto produce IAA after 24 h incubation and the highest production was at 120 h incubation with the concentrationof tryptophan was 2 mg/mL dan 1 mg/mL, respectively. Therefore, it is concluded that Streptomyces spp.isolates are able to produce IAA and potentially to be utilized as biostimulat agent.Keywords: Streptomyces spp., indole-3-acetic acid (IAA), indole-3-acetamide (IAM), Tryptophan Monooxigenasegene (iaaM)
Characterization of Streptomyces spp. Producing Indole-3-acetic acid as Biostimulant Agent de Fretes, Charlie Ester; Sembiring, Langkah; Purwestri, Yekti Asih
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.427 KB) | DOI: 10.22146/ijbiotech.7872

Abstract

Twenty six isolates of Streptomyces spp. obtained from Cyperus rotundus L. rhizosphere were tested forability to produce indole-3-acetic acid (IAA) in yeast malt extract (YM) medium containing 2 mg/mL tryptophan.Screening of the isolates for ability to produce IAA was carried out by adding Salkowski reagent in bacteriaculture and was measured quantitatively by spectrophotometer at λ 530 nm. Thin Layer Chromatography (TLC)method was used to determine IAA. To ensure the IAA production in Streptomyces isolates, gene involved inIAA biosynthesis was detected by amplifying Tryptophan Monooxigenase (iaaM) gene. The study of the effectof tryptophan on the production of IAA was measured at different concentrations of tryptophan (0, 1, 2, 3,4, 5 mg/mL) in the bacterial culture. The result showed that there were two Streptomyces spp. isolates whichcould produce IAA, namely the isolates of Streptomyces sp. MS1 (125.48 μg/mL) and Streptomyces sp. BR27(104.13 μg/mL). The TLC result showed that the compound in both isolates was identifi ed to be IAA. Theamplifi cation results showed that iaaM gene was detected in both isolates. This results indicated that the IAMpathway is predicted involved in the biosynthesis of IAA in the selected isolates. Both of the isolates were ableto produce IAA after 24 h incubation and the highest production was at 120 h incubation with the concentrationof tryptophan was 2 mg/mL dan 1 mg/mL, respectively. Therefore, it is concluded that Streptomyces spp.isolates are able to produce IAA and potentially to be utilized as biostimulat agent. Keywords: Streptomyces spp., indole-3-acetic acid (IAA), indole-3-acetamide (IAM), Tryptophan Monooxigenasegene (iaaM)
Isolasi dan Identifikasi Bakteri Toleran Logam Berat dari Sedimen Mangrove di Pengudang dan Tanjung Uban, Pulau Bintan, Indonesia de Fretes, Charlie Ester; Sutiknowati, Lies Indah; Falahudin, Dede
OLDI (Oseanologi dan Limnologi di Indonesia) Vol 4, No 2 (2019)
Publisher : Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (902.267 KB) | DOI: 10.14203/oldi.2019.v4i2.244

Abstract

Isolation and Identification of Heavy Metals Tolerant Bacteria from Mangrove Sediment in Pengudang and Tanjung Uban, Bintan Island, Indonesia. Bacterial searches for remediation of heavy metal pollutants continue to be carried out due to the increasing pollution in the ecosystem as a result of industrialization. This research aims to identification bacteria strains that are tolerant of heavy metals from mangrove sediments in the Pengudang and Tanjung Uban, Bintan Island in 2018. Bacterial isolation was carried out by pour plate method and purified using marine agar (MA) medium. Screening of bacterial isolates was carried out by growing isolates on MA medium with the addition of 100 ppm Pb. In addition, the determination of minimum inhibitory concentration (MIC) was performed on the isolates were tolerant to Pb, Cu, and Cd by addition of heavy metals in 50-1000 ppm concentrations. Identification of heavy metal tolerant isolates was carried out by sequencing the 16S rRNA gene. The results of bacterial isolation from mangrove sediments obtained 48 isolates used for testing the ability of heavy metals tolerant. Screening tests showed that PGD1A, PGD5A, PGD22A, PGD1B, PGD9B, PGD5C, PGD6C, TJU1, TJU5, and TJU7 isolates were heavy metal resistant. The results showed that bacterial isolated from mangrove sediments had MIC values of 800-900 ppm for Pb, 100-800 ppm for Cu and 100-200 ppm for Cd. There are 3 isolates that have high resistance to three kinds of heavy metals such as Bacillus oceanisediminis PGD1A, Vibrio alginolyticus PGD5A, and Halobacillus kuroshimensis PGD9B. This indicates that the isolate can be used efficiently for removal of heavy metals from the ecosystem.