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SCREENING PENANDA MIKROSATELIT Shorea curtisii TERHADAP JENIS-JENIS SHOREA PENGHASIL TENGKAWANG Nurtjahjaningsih, ILG; Widyatmoko, AYPBC; Sulistyawati, Purnamila; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.49-56

Abstract

Screening primer is an effective method to develop microsatellite markers from related taxa. Aim of this study was to develop microsatellite markers of four Shorea producing tengkawang oil, i.e. Shorea gysbertiana, Shorea macrophylla, Shorea pinanga and Shorea stenoptera by screening microsatellite primers of Shorea curtisii. Leaf samples of the four Shorea were collected from nursery at Center for Forest Biotechnology and Tree Improvement Research in Yogyakarta. Four microsatellite primers of S. curtisii i.e. Shc-1, Shc-2, Shc-7 and Shc-9 had been used to screen. Results showed that numerous alleles were shared among the Shorea. The expected heterozygosity (HE) for locus Shc-1 ranged between 0.594 and 0.722; locus Shc-2 ranged between 0.219 and 0.611; locus Shc-7 ranged between 0.594 and 0.778; and locus Shc-9 ranged between 0.594 and 0.844. Coefficient of inbreeding (FIS) value was low and it was insignificant deviated from Hardy-Weinberg Equilibrium (HWE) at  almost all loci except Shc-1 of S.  pinanga. A dendrogram showed two clusters; S. gysbertsiana and S. macrophylla represented in one cluster, while S. pinanga and S. stenoptera represented in another cluster. Therefore the developed microsatellite markers are possible to be applied for studying population genetics and mating system of these species.
Inter- and intraspecifi c variation of chloroplast mini- and microsatellites DNA in the four closed related Acacia species Widyatmoko, AYPBC; Shiraishi, Susumu
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.538 KB)

Abstract

Mini- and microsatellites of four Acacia species, A. aulacocarpa, A. auriculiformis, A. crassicarpa and A.mangium were investigated on four non-coding regions of cpDNA, the intron of trnL, and the intergenicspacers of trnL - trnP, trnD - trnY, and trnP – trnW. Nine single base substitutions and six informative miniandmicrosatellites were detected in the the four cpDNA non-coding regions. Based on the substitutionsand mini- and microsatellites, ten cpDNA haplotypes (A - J) could be distinguished. Acacia auriculiformispossessed fi ve haplotypes, A. aulacocarpa, four haplotypes, and A. crassicarpa, three haplotypes. All samplesof A. mangium possessed the same haplotype. Mini- and microsatellites recognized in this study can beused for species identifi cation of the four Acacia species. The ten haplotypes could divided the four speciesinto 2 groups, A. aulacocarpa-A.crassicarpa group and A. auriculiformis-A. mangium group. By developing thePCR-based markers based on the sequence information, many experiments can be carried out for the Acaciaimprovement programs.
KERAGAMAN GENETIK EMPAT POPULASI lntsia bijuga BERDASARKAN PENANDA RAPD DAN IMPLIKASINYA BAGI PROGRAM KONSERVASI GENETIK Rimbawanto, Anto; Widyatmoko, AYPBC
Jurnal Penelitian Hutan Tanaman Vol 3, No 3 (2006): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpht.2006.3.3.149-154

Abstract

Intsia bijuga atau merbau merupakan jenis kayu bemilai ekonomi tinggi dan telah mengalami eksploitasi  yang intensif. Penelitian ini bertujuan mempelajari keragaman genetik populasi merbau guna membantu penyusunan strategi konservasi genetik, dengan menggunakan penanda RAPD. Sampel daun dikumpulkan dari 4 populasi dan dianalisa menggunakan 15 primer RAPD yang menghasilkan 77 lokus polimorfik. Rata-rata lokus polimorfik per primer adalah 5.1. Nilai keragaman genetik rerata dalam populasi sebesar 0,296 sedangkan keragaman antara populasi 0.141. Analisis klaster membagi keempat populasi menjadi dua kelompok populasi yaitu Carita dan Manokwari pada kelompok pertama, sedangkan kelompok kedua terdiri dari populasi Temate dan Nabire. Pembagian kelompok antara Manokwari  dan Nabire lebih memperjelas pembagian Papua menjadi 6 wilayah geogenetik.
DISTRIBUSI KERAGAMAN GENETIK POPULASI Santalum album BERDASARKAN PENANDA RAPD Rimbawanto, Anto; Widyatmoko, AYPBC; Sulistyowati, Purnamila
Jurnal Penelitian Hutan Tanaman Vol 3, No 3 (2006): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpht.2006.3.3.175-181

Abstract

Santalum album atau yang dikenal dengan nama cendana merupakan jenis kayu bemilai  tinggi dan telah mengalami degradasi sumber genetik yang serius. Jenis ini merupakan tanaman asli Propinsi Nusa Tenggara Timur. Penelitian ini bertujuan untuk mengetahui keragaman  genetik dan hubungan kekerabatan  populasi Santalum  album untuk mendukung  program konservasi dan pemuliaan jenis tersebut. Sampel daun dikumpulkan dari 17 populasi dan dianalisa menggunakan 17 primer RAPD yang menghasilkan 34 lokus polimorfik. Rata-rata lokus polimorfik per primer adalah 2. Nilai rata- rata keragaman genetik dalam populasi sebesar 0,391 sedangkan keragaman  antara populasi 0,038. Analisis klaster membagi 17 populasi menjadi dua kelompok besar. Secara umum pembagian kelompok tidak memperlihatkan hubungannya denganjarak geografis, tetapi populasi-populasi yang berdekatan mempunyai kecenderungan untuk membentuk satu  sub-kelompok.
KERAGAMAN POPULASI Eusideroxylon zwageri KALIMANTAN TIMUR BERDASARKAN PENANDA RAPD Rimbawanto, Anto; Widyatmoko, AYPBC; Harklngto, Harklngto
Jurnal Penelitian Hutan Tanaman Vol 3, No 3 (2006): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpht.2006.3.3.201-208

Abstract

Eusideroxylon zwageri  atau ulin adalah kayu bemilai ekonomi tinggi dan telah mengalami eksploitasi yang intensif sehingga keberadaan tegakan ulin di hutan alam semakin langka. Penelitian ini bertujuan mempelajari keragaman genetik populasi ulin di Kalimantan Timur guna membantu program konservasi genetik, dengan menggunakan penanda RAPD. Sampel daun dikumpulkan dari 5 populasi dan dianalisa menggunakan 19 primer RAPD  yang menghasilkan 48 lokus polimorfik. Nilai keragaman  genetik rerata dalam populasi sebesar 0.3564 sedangkan keragaman antara populasi 0.0415. Analisis AMOVA menunjukkan  bahwa 96% dari keragaman genetik terdapat di dalam populasi, sedang sisanya ada di antara populasi. Analisis klaster menghasilkan dua kelompok populasi yaitu TN Kutai, Meratus, S.Wain dan Semboja, sedang populasi Lempake satu kelompok tersendiri.
Keragaman Genetik Lima Populasi Gyrinops verstegii di Lombok menggunakan Penanda RAPD Widyatmoko, AYPBC; Afritanti, Rizki Diah; Taryono, Taryono; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 1 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2009.3.1.1-10

Abstract

Gyrinops verstegii is one of the species which produce high economic value of gaharu (agarwood). The objectives of this research were to estimate genetic diversity within and among populations, investigate distribution of genetic diversity, and genetic relationship among five populations of G. Verstegii. Leaf samples were taken from five populations of G. Vertegii in Lombok, NTB,. Genetic diversity was analyzed using 21 selective primers out 200 primers. A total of 42 polymorphic loci were detected. Mean of genetig diversity was 0.288, and genetic distance betwen populations was 0.148. The Proportion of 85.24 % of genetic diversity was distributed within population, and the remaining 14.76 % was distributed between populations. Based on cluster analysis, the five populations were separated into two major group consisted of Pusuk Pohon Induk, Pusuk Sumber Benih, and Mantang I. The second group consisted of Mantang Pohon Induk and Mantang II. Collecting genetic materials for conservation and tree improvement program should be focused on the materials within population and each group (regent) should be a representative of at least one population.
Keragaman Genetik dan Hubungan Kekerabatan pada Tiga Jenis Aquilatria Menggunakan Penanda RAPD Widyatmoko, AYPBC; Ariningsih, Elsih Dian; Prasetyaningsih, Aniek
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.3.139-148

Abstract

Gaharu is an imprortant species of non wood forest product of Aquilaria spp with hight economic value. The natural stand has been exploited very intensive. The aim of the study were to investigate level of Aquilaria (namely A. malaccensis, a. beccariana and A. microcarpa) in order to provide genetic information for developing conservation strategy of the species. Aamples were colleted from 7 populations and analyzed using 26 RAPD primers. Total of 84 polymorpic loci have been obtained from these primers. Mean of genetic diversity within population indicated by A. malaccensis Muara Bungo population. A. malaccensis Berau population has the lowest genetic diversity. The highest genetic distance between populations was between A. micricarpa samboja and A. beccariana Berau. In contrast, genetic distance between A. Microcarpa Bearu and A. malaccensis Berau was the the lowest (0.017). based on Cluster analysis, The seven populations were divided into 2 groups. The first group consisted of 2 populations of A. malaccensis and A. microcarpa from Kalimantan.
KARAKTERISASI KERAGAMAN GENETIK POPULASI JABON PUTIH MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA Nurtjahtjaningsih, ILG; Qiptiyah, Maryatul; Yudohartono, Tri Pamungkas; Widyatmoko, AYPBC; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 2 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.2.81-92

Abstract

Anthocepalus cadamba (white jabon) has high economical value for furniture. White jabon forests severely degraded due to intensive exploitation and land conversion. Genetic diversity is one of important consideration to design conservation and improvement strategies. Aim of this study was to access the genetic diversity values within and among population of white jabon. Leaf samples of white jabon were collected from conservation plots originated from West Lombok, Sumbawa, South Sumatera and West Sumatera. Red jabon was included as an outgroup population. Based on 37 polymorphic RAPD loci, the results showed comparable value of genetic diversity between white jabon and red jabon. Number of detected and rare alleles was highest founded in Sumbawa population among the other three populations of white jabon. As consequence, value of expected heterozygosity in the population was highest (HE=0.315). Private allele was only detected in South Sumatera population. Principal coordinate analysis (PCA) showed that integrating between genetic and geographical distance was inconsistent; similar gene resources or human impact might be responsible for this result. The populations that have high value of genetic diversity and private allele are recommended to be selected for the conservation strategies, i.e. Sumbawa and South Sumatera.
HUBUNGAN KEKERABATAN ANTAR POPULASI JATI (Tectona Grandis,Linn.F.) BERDASARKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Widyatmoko, AYPBC; Rimbawanto, Anto; Chasani, Abdul Razaq
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 3 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2013.7.3.151-166

Abstract

Information on genetic relationship amongst teak (Tectona grandis Linn.f) populations in Indonesia is important, in order to maintain the high genetic diversity of the species and to ensure the origin of commercial planting materials. In this study, 94 loci from 25 RAPD primers were used for analyzing genetic diversity and genetic relationship among 30 populations of teak which collected from provenance trial in Bojonegoro and its populations in Sulawesi. Mean genetic diversity (h) of the 30 populations was 0.184, and mean genetic distance between populations (D) was 0.441. Based on cluster analysis, 30 populations of teak were divided into 3 groups. The first group consisted of Burma, the second group consisted of Jawa, India, Indochina and Thailand populations, and the third group consisted of all  populations in Sulawesi. High genetic distance between Jawa’s and Sulawesi’s populations can be used to  differentiate seed/seedling from both regions.
KARAKTERISTIK PEMBUNGAAN DAN SISTEM PERKAWINAN NYAMPLUNG (Calophyllum inophyllum) PADA HUTAN TANAMAN DI WATUSIPAT, GUNUNG KIDUL Nurtjahjaningsih, ILG; Sulistyawati, Purnamila; Widyatmoko, AYPBC; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 2 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.2.65-78

Abstract

Flowering are influenced by internal factors, such as genetic and phytohormone, and environment factors, such as sunlight and nutrition intake. The flowering characteristics influence fruiting and genetic diversity seedlings through mating systems. This study aims to assess flowering and fruiting characteristics and to determine pattern of mating system of a Calophyllum inophyllum plantation at Watusipat, Gunung Kidul. Flowering and fruiting were observed at 4 locations, 3 parts of crown, and 4 main directions to know the effects of sunlight, nutrition intake and phytohormone in the flowering process. Mating system was assessed by comparing genetic diversity values between parent trees and offsprings. The values of genetic diversity were analyzed using 5 RAPD primers with 17 polymorphic loci. Analysis of variant showed that the locations, crown parts, directions and interaction between a location and direction significantly affected to differences number of flowers and fruits. Values of genetic diversity (h) of parent trees ranged between 0.1471 and 0.3056. The values increased at almost overall offsprings; it ranged between 0.2864 and 0.3750. Values of genetic distance (Da) between parent trees were high and very high (0.197 – 0.364), but the values was decreased between parent trees and their offspring, even between offspring populations. A dendrogram showed two main clusters; first cluster consisted parent trees at up edge with rare trees and second cluster consisted sub cluster parent trees at up edge; sub cluster parent trees at down middle; and sub cluster parent trees at down edge and overall offsprings. Flowering/ fruiting characteristics and pattern of mating systems of C. inophyllum were briefly discussed. Key words : Flowering characteristic, genetic diversity, Calophyllum inophyllum, RAPD analysis