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In vitro Antiplasmodial Activity and Cytotoxicity of Vincadifformine and Its Semisynthetic Derivatives M, Mustofa; Mallié, Michèle; Valentin, Alexis; Lewin, Guy
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

An indole alkaloid with aspidospemane structure possessing a potential antiplasmodial activity,vincadifformine, has been isolated from Aspidosperma pyrifolium Mart. Moreover, 10 derivatives were preparedfrom the vincadifformine. The study was conducted to evaluate the in vitro antiplasmodial and cytotoxic activity ofthe vincadifformine and their semisynthetic derivatives. The in vitro antiplasmodial activity was evaluated onPlasmodium falciparum chloroquine-resistant (FcM ) and –sensitive (Nigerian) strains after 24-h and 72-h incubation, 29while cytotoxic activity was estimated on Hela cells and Cytotoxicity Index (CI = IC on HeLa cells/IC on FcM strain) 50 50 29was calculated to evaluate the safety of tested compounds. Experiment results showed that two compounds (4 and 8)exhibited good antiplasmodial activities in comparison with parent compound, vincadifformine and other testedcompounds with IC ranging from 5.3 to 12.8 μM on FcM strain and 11.4 to 24.0 μM on Nigerian strain. In addition, 50 29the CI of two compounds were also lower after 24-h incubation (CI, 2.0 and 4.8) than that of after 72-h incubation (CI,9.5 and 11.5). Further study will be conducted to evaluate quantitative structure-activity relationship (QSAR) in orderto design new antimalarial drugs.Keywords : vincadifformine - antiplasmodial – Plasmodium falciparum – cytotoxic - HeLa
Antifungal Production of a Strain of Actinomycetes spp Isolated from the Rhizosphere of Cajuput Plant: Selection and Detection of Exhibiting Activity Against Tested Fungi A, Alimuddin; Widada, Jaka; Asmara, Widya; M, Mustofa
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

Actinomycetes are bacteria known to constitute a large part of the rhizosphere microbiota. Their isolation is an important step for screening of new bioactive compounds. Culturable actinomycetes populations from cajuput plant rhizosphere soils in Wanagama I Forest UGM Yogyakarta were collected to study about their antifungal activity. Among 17 of a total 43 isolates that showed activity were screened for producing antifungi substances. Screening for antifungal activity of isolates were performed with dual culture bioassay in vitro. One isolate that was designated as Streptomyces sp.GMR-22 was the strongest against all tested fungi and appeared promising for a sources of antifungal. Culture’s supernatant and mycelia were extracted with chloroform, ethyl acetate and methanol, respectively. Antifungal activity of crude extracts was tested by diffusion method against tested fungi. The result indicates that isolates of actinomycetes from cajuput plant rhizosphere could be an interesting sources of antifungal bioactive substances.
PERSEPSI PEMUSTAKA TERHADAP PENGEMBANGAN INSTITUTIONAL REPOSITORY DI PERPUSTAKAAN ISI SURAKARTA Setiyono, Joko; M, Mustofa
Publication Library and Information Science Vol 3, No 1 (2019)
Publisher : UPT. Perpustakaan Universitas Muhammadiyah Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24269/pls.v3i1.1576

Abstract

Repositori institusi merupakan hal yang penting menjadi rujukan dalam pembangunan wacana ilmiah dan akademik. Peringkat repositori institusi ISI Surakarta pada tahun 2017 berada di peringkat ke-87 dari semua universitas di Indonesia. Posisi merosot ke 120 pada 9 April 2018, fakta ini menunjukkan bahwa ada kebutuhan untuk menilai, mengevaluasi, mengembangkan dan meningkatkan kualitas repositori institusional di ISI Surakarta. Dengan menggunakan metode penelitian kuantitatif, tujuan penelitian ini adalah: 1) Untuk mengetahui penggunaan repositori institusional di ISI Surakarta oleh pemustaka. 2) Untuk menentukan keuntungan dan kerugian dari repositori institusional di ISI Surakarta oleh pengguna. 3) Untuk mengetahui perkembangan repositori institusional di ISI Surakarta oleh pemustaka.