Regina TC. Tandelilin, Regina TC.
Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada

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Derajat Keasaman (pH) Saliva Meningkat Setelah Berkumur Rebusan Daun Sirih Merah (Paper Crocatum) Konsentrasi 10% pada Lansia Penderita Diabetes Melitus Tipe 2 Tandelilin, Regina TC.; Sandy, Leny Pratiwi Arie; Hondro, Meydistin Juwita
Jurnal Teknosains Vol 9, No 2 (2020): June (FORTHCOMING)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.43897

Abstract

Dental caries is the major dental and oral health problems in Indonesia and world wide. Alteration in the saliva acidity level (pH) in the oral environment plays an important role in dental caries. Red betel leaf (piper crocatum) is a herbal plant that commonly found in Indonesia. Red betel leaf contains alkaloid, flavonoid, tannin and essential oil which have antibackterial activities. Objectives : The aim of this study was to determine the acidity change (pH) of saliva in elderly people with diabetes mellitus type 2 after gargling of 10% boiled red betel leaves.Methods : the subjects of this study were 30 persons who divided randomly into two groups i.e treatment group and control group. Each group consisted of 15 persons. In the treatment group, the subjects gargled using 10% boiled red betel leaves, while in the control group the subject gargled using aquades. The saliva was collected on each time treated and followed by pH measuring. The data analysis was conducted by Independent t-test statistic (p≤0,05).Results : the results showed that there was an effect of increasing salivary acidity (pH) of saliva after gargling boiled of red betel leaf (Piper crocatum) in elderly people with diabetes mellitus type 2. Conclusion : The change of acidity level (pH) of saliva was increased significantly at 5th to 15th minute from 5.33 to 6.67 after gargling boiled red betel leaves concentration of 10%. The acidity level range stated above indicates a normal. Key words : pH of saliva, red betel leaves, elderly, diabetes mellitus type 2
EFEK EKSTRAK DAUN TEH (Camellia sinensis) KONSENTRASI 0,5% TERHADAP KADAR sIgA PADA SALIVA PENDERITA GINGIVITIS Handajani, Juni; Asmara, Widya; Tandelilin, Regina TC.
Journal of Dentistry Indonesia Vol 11, No 1 (2004): April
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3049.658 KB) | DOI: 10.14693/jdi.v11i1.627

Abstract

Enhanced humoral immune response (sIgA) can be caused by gingivitis. A study has been conducted on the effect of tea leaf extract (Camellia sinensis) at a concentration of 0,5% as mouth-wash on salivary sIgA level on gingivitis patients. Ten patients (age= 18-30 years) were divided into 2 groups so that five patients of the first group representes early gingivitis, and five patients of the second group represented normal gingiva. The gingival index by Loe and Silness (1963) was measured one day before treatment and five days after treatment. The first group was given tea leaf extract solution (0,5%) and the others were given aquabidest as control. Five ml of each solution was mouth washed for 5 seconds and this was repeated two times in the morning and at night before sleeping. All treatments were repeated for five days. Saliva samples were collected one day before treatment and five days after treatment. The sIgA level was measured using sIgA ELISA kit (Salimetrics LLC). Optical Density was read on standard plate at 450 nm. The results showed a significant decrease in the salivary sIgA level and in the severity of gingivitis by mouth-washing with a tea leaf extract at a concentration 0.5%.
The density of collagen fiber in alveolus mandibular bone of rabbit after augmentation with powder demineralized bone matrix post incisivus extraction Tandelilin, Regina TC.; M. Sofro, Abdul Salam; Santoso, Al Supartinah; Soesatyo, Marsetyawan HNE; Asmara, Widya
Dental Journal (Majalah Kedokteran Gigi) Vol 39, No 2 (2006): (June 2006)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.688 KB) | DOI: 10.20473/j.djmkg.v39.i2.p43-47

Abstract

The bone defect due to tooth extraction contributes the most cases reported in the aspects of oral surgery. The defect can be preventively managed by adding powder bone matrix intended for augmentation which eventually induces the formation of new bones. This hard tissue wound healing is preceded by the presence of collagen fibers. The aim of this study was to determine the density of collagen fiber in the alveolus mandibular bone of rabbit which was augmented using powder demineralized bone matrix (DBM) post incisivus extraction. Twenty four male rabbits aged 2.5–3 months weighed 900–1,100 grams were randomly divided into two groups. The treated rabbits were augmented with DBM after the incisivus extraction on mandible. The mucosa was then sutured. On the other hand, the controlled rabbits received similar treatments with those of the treated rabbits except there was no augmentation of DBM. Decapitation of treated and controlled rabbits was made on day 5, 7, 10, and 14 days post surgery, each with three rabbits. Mandibles were cut, decalcified, and imbedded in paraffin block. The staining was done using Mallory. Significant differences in the density of collagen were noted on day 10 and 14 post surgery, indicating that powder demineralized bone matrix successfully induced the stimulation of collagen.
Micronucleus frequency in exfoliated buccal cells from hairdresser who expose to hair products Yee, Koh Hui; Jonarta, Alma Linggar; Tandelilin, Regina TC.
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.311 KB) | DOI: 10.20473/j.djmkg.v48.i2.p74-79

Abstract

Background: Hairdresser is one of the fastest growing occupations in today’s society. Hairdresser help styling, cutting, colouring, perming, curling, straightening hair and various treatment to customer. Somehow, hairdresser are constantly exposed to chemical substances such as aromatic amines, hydrogen peroxide, thioglycolic acid, formaldehyde in hair products which can cause damage to human’s genome. Micronucleus is one of the effective biomarker for processes associated with the induction of DNA damage. Purpose: The aim of this study was to determine the micronucleus frequencies in buccal mucosa epithelial cells of hairdresser who were exposed to chemical of hair products. Method: This study was conducted on twenty female subjects, who were divided into 2 groups: exposed and non-exposed (control) group. All subjects recruited were working in the same beauty salon. Buccal cells were obtained from each individual by using cytobrush. The cells were stained with modified Feulgen-Ronssenback method and counting of micronucleus per 1000 cell was done under light microscope. The data were analyzed using independent t-test and one-way Anova (p<0.05). Result: The result showed a significant difference in micronucleus frequency between 2 groups. There were a significantly increase of micronucleus frequency in hairdressers and increase of  micronucleus frequency with the longer duration of exposure. Conclusion: It concluded that the chemical substances of hair products had affected the micronucleus frequency ofthe epithelial cells in buccal mucosa of hairdressers.
Streptococcus Alpha growth in gingivitis patient’s dental plaque after rinsing with green tea extract (Camellia Sinensis) Kurniwati, Isti Endah; Handajani, Juni; Tandelilin, Regina TC.
Padjadjaran Journal of Dentistry Vol 19, No 1 (2007): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.891 KB) | DOI: 10.24198/pjd.vol19no1.14174

Abstract

Green tea (Camellia sinensis) has been widely known as a healthy drink since long time ago. One of the substances in green tea which can give health benefit is catechin, an antibacterial substance. The purpose of this research is to know the efficacy of rinsing with green tea extract to Streptococcus alpha growth on gingivitis patient’s dental plaque. The research subjects include 30 mild gingivitis patients, and these subjects are divided into two groups: treatment group (20 patients) and control group (10 patients). Ten patients of treatment group rinse their mouth with 0.25% green tea extract and 10 other patients with 0.5% green tea extract. Meanwhile, control group use Bactidol (0.1% Hexetidine). Rinsing the mouth is performed every morning and night for five days. Sampling is conducted on the first and sixth day. Before rinsing data is analyzed using ANOVA and the result shows a significant difference. ANAVA testing then is done using proportion value. ANAVA Testing result shows that there is no significant difference among the patients in the treatment group. This result indicates that the effect of rinsing with 0.25% and 0.5% green tea extract is equivalent to the control (0.1% Hexetidine) to inhibit S. alpha growth on mild gingivitis patient’s dental plaque. From these two concentrations, it has not been known which one is the most effective concentration to inhibit S. alpha growth on mild gingivitis patient.
Streptococcus Alpha growth in gingivitis patient’s dental plaque after rinsing with green tea extract (Camellia Sinensis) Kurniwati, Isti Endah; Handajani, Juni; Tandelilin, Regina TC.
Padjadjaran Journal of Dentistry Vol 19, No 1 (2007): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.891 KB) | DOI: 10.24198/pjd.vol19no1.14174

Abstract

Green tea (Camellia sinensis) has been widely known as a healthy drink since long time ago. One of the substances in green tea which can give health benefit is catechin, an antibacterial substance. The purpose of this research is to know the efficacy of rinsing with green tea extract to Streptococcus alpha growth on gingivitis patient’s dental plaque. The research subjects include 30 mild gingivitis patients, and these subjects are divided into two groups: treatment group (20 patients) and control group (10 patients). Ten patients of treatment group rinse their mouth with 0.25% green tea extract and 10 other patients with 0.5% green tea extract. Meanwhile, control group use Bactidol (0.1% Hexetidine). Rinsing the mouth is performed every morning and night for five days. Sampling is conducted on the first and sixth day. Before rinsing data is analyzed using ANOVA and the result shows a significant difference. ANAVA testing then is done using proportion value. ANAVA Testing result shows that there is no significant difference among the patients in the treatment group. This result indicates that the effect of rinsing with 0.25% and 0.5% green tea extract is equivalent to the control (0.1% Hexetidine) to inhibit S. alpha growth on mild gingivitis patient’s dental plaque. From these two concentrations, it has not been known which one is the most effective concentration to inhibit S. alpha growth on mild gingivitis patient.