Rania Bakry, Rania
Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, Innrain 52a, A-6020, Innsbruck

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POLY(GLYCIDYL METHACRYLATE-DIVINYLBENZENE) MONOLITHIC CAPILLARY AS A STATIONARY PHASE FOR THE REVERSED-PHASE CHROMATOGRAPHIC SEPARATION OF PROTEINS Aprilita, Nurul Hidayat; Bakry, Rania; Huck, Christian W; Bonn, Guenther K
Indonesian Journal of Chemistry Vol 5, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.418 KB) | DOI: 10.22146/ijc.21830

Abstract

                Capillary column with monolithic stationary phase was prepared from silanized fused-silica capillary of 200 µm I.D. by in situ free radical polymerization of divinylbenzene with glycidy methacrylate in the presence of decanol and tetrahydrofuran as porogens.  The hydrodynamic and chromatographic properties of this monolith, such as backpressure at different flow-rate, pore size distribution, van Deemter plot and the effect of varying gradient-rate were investigated.  Poly(glycidyl methacrylate-divinylbenzene) monolithic capillary has been used successfully for the reversed-phase chromatographic separation of proteins.   Keywords: monolithic stationary phase, poly(glycidyl methacrylate-divinylbenzene), proteins