Sularsih Sularsih, Sularsih
Department of Dental Materials and Technology, Dentistry Faculty, Hang Tuah University

Published : 4 Documents
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Evaluation in Accuracy to Two Impression Techniques: In Case of Bone Anchored Bridge Sularsih, Sularsih; Ishida, O.; Ashrin, Meinar Nur; Okajima, M.; Ichikawa, T.
Journal of Dentistry Indonesia Vol 17, No 1 (2010): April
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5033.547 KB) | DOI: 10.14693/jdi.v17i1.111

Abstract

Purpose: The purpose of this study was to investigate and compare the accuracy of two procedures: open tray with splinting technique and closed tray impression techniques, in innovative in vitro experiments. Materials and Methods: One master cast was fabricated with 4 abutment replica implants with almost parallel position in an anterior region of an edentuloys mandibular plaster cast. The working cast was taken impessions with open tray splinting technique (group 1) and closed tray technique (group 2) using polyvinyl siloxane impression material. The Type III dental stone ws poured into around the impressions. The accuracy of impression procedured were measured 24 hours long after pouring dental stone to each impression. Four sited were marked to measured on every platform of implant analogs. The analyzing stylus was positioned to each site and the heights, horizontal inclination and saggital incliniation were measured using the Laser displacement transducer (LK G115; Keyence, Osaka, Japan). Measurements of these 16 points of four implants per a model was repeated 7 times under the same condition. The gap between the abutment and superstructure at one screw test was evaluated with a digital microscope system (VH-Z100 & VH-5000; Keyence, Osaka, Japan). The mean and standard deviation estimated from the samples of each subgroup were statistically analyzed by ANOVA test (P<0.05 as the level of significance). Results: The relative differences of implant heights, horizontal inclination and saggital inclination of each implant on closed tray technique was statistically different to master cast and open tray with splinting (P<0.005). Conclusion: The open tray technique was more accurate comparing to the closed tray technique. The gap between the abutment and superstructure in the open tray with splinting technique was smaller comparing to closed tray technique.DOI: 10.14693/jdi.v17i1.111
Degrees of chitosan deacetylation from white shrimp shell waste as dental biomaterials Sularsih, Sularsih; Yuliati, Anita; D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 45, No 1 (2012): (March 2012)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.046 KB) | DOI: 10.20473/j.djmkg.v45.i1.p17-21

Abstract

Background: Chitosan is biomaterial improved for various dentistry applications because it is biocompatible, degradable, nontoxic, and not carcinogenic. The main parameter affecting the characteristics of chitosan is deacetylation degree. Purpose: This study is aimed to determine the degree of deacetylated of chitosan derived from white shrimp shell waste used as dental biomaterial. Methods: White shrimp shells were crushed into powder. Next, deproteination process was conducted with 3.5% NaOH solution, demineralized with 1N HCl solution, and then depigmented with 90% acetone solution into chitin powder. Deacetylation process was then conducted by soaking the chitin powder in 50% NaOH solution for 6 h at 65° C to produce white powder of chitosan. Afterwards, deacetylation degree test was conducted by using Fourier Transform Infrared Spectrophotometer (FTIR) to calculate the ratio of the absorption bands between the absorbance peak of amide group about 1655 cm–1 and the absorbance peak of hydroxyl group about 3450 cm–1. Results: The result of the deacetylation degree test on the chitosan powder derived from white shrimp shell waste was high, about 85.165%, and had the eligible form, solubility, and pH. Conclusion: It can be concluded that the deacetylation degree of chitosan from white shrimp shells could reach 85.165%.Latar belakang: Kitosan merupakan biomaterial yang dikembangkan untuk berbagai aplikasi kedokteran gigi karena biokompatibel, dapat didegradasi, tidak toksik dan tidak karsinogenik. Parameter utama yang mempengaruhi karakteristik kitosan adalah derajat deasetilasi. Tujuan: Tujuan dari penelitian ini adalah mengetahui derajat deasetilasi kitosan dari limbah kulit udang putih sebagai biomaterial kedokteran gigi. Metode: Kulit udang putih dihaluskan menjadi serbuk. Setelah itu dilakukan proses deproteinasi dengan larutan NaOH 3,5%, demineralisasi dengan larutan HCl 1N, depigmentasi dengan larutan aseton 90% sehingga menjadi serbuk kitin. Proses deasetilasi dilakukan dengan merendam serbuk kitin dalam larutan NaOH 50% selama 6 jam pada suhu 65° C sehingga dihasilkan serbuk putih kitosan. Uji derajat deasetilisasi menggunakan metode spektrofotometer Fourier Transform Inframerah (FTIR) dengan menghitung nilai perbandingan pita serapan antara puncak absorbansi gugus amida sekitar 1655 cm–1, dan puncak absorbansi gugus hidroksil sekitar 3450 cm–1. Hasil: Hasil uji derajat deasetilasi serbuk kitosan dari limbah kulit udang putih adalah tinggi yaitu sebesar 85.165% dan memiliki bentuk, kelarutan dan pH yang memenuhi syarat. Kesimpulan: Dapat disimpulkan derajat deasitilasi kitosan dari kulit udang putih adalah 85,165%.
Expression of bone morphogenetic protein-2 after using chitosan gel with different molecular weight on wound healing process of dental extraction Sularsih, Sularsih; Wahjuningsih, Endah
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1399.431 KB) | DOI: 10.20473/j.djmkg.v48.i2.p53-58

Abstract

Background: Bone morphogenetic protein-2 (BMP-2) is bone stimulator which capable of inducing differentiation of mesenchymal cells into osteoblast, stimulating bone formation in wound healing process of dental extraction. Chitosan is polymer composed N-acetylD-glucosamine unit that has been used in various applications in wound healing process and bone tissue engineering. Purpose: The objective of this research was to analyzed expressions of BMP-2 for 7,14 and 21 days after using chitosan gel with different molecular weight on wound healing process of dental extraction. Method: The research was an experimental laboratory study. Rattus nornegicus strain wistar male, aged 8-16 weeks, divided into 3 treatment groups namely group 1 and II which given chitosan gel 1 % with high and low molecular weight and group III as control which were not given chitosan gel. Chitosan gel were applied into the socket of dental extraction. Rat was decaputated 7,14 and 21 days after chitosan gel application and the jaw in the treated regions and control group were cut for immunohistochemical examination to observe BMP-2. Data were analyzed using ANOVA test. Result: The result of this research showed significant differences on BMP-2 for 7,14 and 21 days observation (p<0,05). The increasing of BMP-2 were found in the group which given chitosan gel with high molecular weight. Conclusion: It may be concluded that chitosan gel with high molecular weight can enhance the expresion of BMP-2 on wound healing process ofdental extraction.
Perbedaan Pengaruh Aplikasi Gel Kombinasi Kitosan Berat Molekul Tinggi dan Rendah dengan Ekstrak Lidah Buaya (Aloe vera) Terhadap Kepadatan Kolagen pada Proses Penyembuhan Ulkus Traumatikus Suhartono, Michelle; Sularsih, Sularsih; Nafi?ah, Nafi?ah
DENTA Jurnal Kedokteran Gigi Vol 12, No 1 (2018)
Publisher : FKG Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v12i1.156

Abstract

Background: Traumatic ulcer is one of the most common oral wounds. Chitosan has mucoadhesive characteristic while Aloe vera containing lignin which is able to penetrate the skin. It is expected that the combined gel of chitosan and Aloe vera will function as wound healing accelerator in traumatic ulcer. Molecular weight is one of the characteristics of chitosan quality. Purpose: the aim of this experiment was to know the density of collagen fibers in wound healing of traumatic ulcer using the combined gel of chitosan with different molecular weight and Aloe vera. Materials and method: 30 Male Rattus Norvegicus were divided into 3 groups. Group I was control group (without chitosan and Aloe vera), group II was given low molecular weight chitosan and Aloe vera, group III was given high molecular weight chitosan and Aloe vera. The groups were given traumatic ulcer making with 4 mm diameter and 2 mm depth. Rats were sacrificed by decapitation on day 3 and 7 then they were examined histopatologically to see the density of collagen fibers. Result: Statistical analysis with Kruskall Wallis and Mann-Whitney U test showed that there were significant difference p&lt;0,05 between high and low molecular weight chitosan with Aloe vera group on day 3 and 7. Conclusion: Chitosan with high molecular weight and Aloe vera were more effective in wound healing of traumatic ulcer because they increase the density of collagen fibers.?Keywords:? Combined gel of chitosan and Aloe vera, density of collagen fibers, wound healing.?Korespondensi: Sularsih, Bagian Ilmu Biomaterial Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Hang Tuah, Arif Rahman Hakim 150, Telepon 031-5912191.