Roro Wahyudianingsih, Roro
Bagian Patologi Anatomi, Fakultas Kedokteran, Universitas Kristen Maranatha
Articles
4
Documents
The Effect of Chicken (Gallus gallus domesticus) Eggshell’s Powder towards Wound Healing of Incision Wound on Male Swiss-Webster Mice

Journal of Medicine and Health Vol 1, No 4 (2016)
Publisher : Maranatha Christian University

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Abstract

Domestic chicken eggshells contain calcium and protein that could be useful in wound healing process. This research was conducted to determine whether domestic chicken eggshells can accelerate wound healing. Twenty one male Swiss-Webster mice  with 2 cm incision were divided into 3 groups. Negative control group were given Carboxymethyl cellulose 1% (CMC), positive control group were given feracrylum 1% (FER), and the eggshells group given domestic chicken eggshells powder (KT). Treatment and data mesurement was measured every day until  the 7th day. On the 7th  day the average length of the wound were analyzed and the skin tissue is taken for microscopic examination. Data analyzed using one-way ANOVA followed by Post Hoc Least Significant Difference (LSD) with p=0,05. On macroscopic examination, there were  highly significant differences between KT compared to CMC and highly significant differences between KT compared to FER. the average length of wound on the 7th day on KT 0,63 cm shorter than CMC 1,07 (p=0,000) and FER 0,81 cm (p=0,003). On microscopic examination, the degree of reepithelization on KT groups are severe, moderate for collagen density, fibroblasts density, subepithelial neutrophil density, degree of edema, and mild for angiogenesis. We conclude domestic chicken eggshells powder can accelerate wound healing. Key words: domestic chicken eggshells, incision wound, wound healing

EKSTRAK KEDELAI DETAM 1, DAUN JATI BELANDA SERTA KOMBINASINYA TERHADAP BERAT BADAN DAN HISTOPATOLOGIS HEPAR TIKUS WISTAR

JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 6, No 4, (2015)
Publisher : Faculty of Medicine

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Abstract

Latar Belakang Biji kedelai Detam 1 dan daun jati Belanda berefek menghambat kenaikan berat badan, akan tetapi dikhawatirkan mempengaruhi organ hepar. Tujuan penelitian Tujuan penelitian untuk mengetahui efek ekstrak etanol biji kedelai Detam 1 (EEKD), ekstrak etanol daun jati Belanda (EEJB) dan kombinasinya terhadap penghambatan kenaikan berat badan dan gambaran histopatologis hepar pada tikus Wistar yang diberi pakan tinggi lemak (PTL). Metode penelitian Penelitian merupakan eksperimental laboratorium dengan rancangan acak lengkap bersifat komparatif. Sebanyak 40 ekor tikus Wistar jantan dibagi secara acak menjadi 8 kelompok perlakuan, masing masing terdiri dari 5 ekor. Selanjutnya diberi perlakuan selama 28 hari, semua kelompok kecuali kelompok kontrol negatif (KN), tetap diberi PTL. Pada hari ke-29, seluruh tikus dikorbankan dan semua hepar tikus, kecuali kelompok Orlistat (K6), dibuat sediaan histopatologis dengan pewarnaan Haematoxylin Eosin (HE). Hasil Penghambatan kenaikan berat badan terjadi pada semua kelompok perlakuan, kelompok K3 (EEKD 10 mg : EEJB 20 mg) menunjukkan penghambatan kenaikan berat badan yang paling baik dan potensinya setara dengan kontrol positip (KP) atau Orlistat. Pada semua kelompok perlakuan (K1, K2, K3, K4 dan K5) terjadi perubahan struktur arsitektur dan inflamasi di daerah portal namun tidak menyebabkan bengkak keruh dan degenerasi lemak. Kesimpulan: Pemberian kombinasi EEKD 10 mg : EEJB 20 mg menunjukkan penghambatan kenaikan berat badan yang paling baik. EEJB sediaan tunggal menyebabkan perubahan gambaran histopatologis hepar paling buruk pada tikus Wistar jantan yang diinduksi pakan tinggi lemak. Kata kunci : kedelai Detam 1, jati Belanda, berat badan, histopatologis hepar, Tikus Wistar

The Effect of Chicken (Gallus gallus domesticus) Eggshell’s Powder towards Wound Healing of Incision Wound on Male Swiss-Webster Mice

Journal of Medicine & Health Vol 1, No 4 (2016)
Publisher : Universitas Kristen Maranatha

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1015.363 KB)

Abstract

Domestic chicken eggshells contain calcium and protein that could be useful in wound healing process. This research was conducted to determine whether domestic chicken eggshells can accelerate wound healing. Twenty one male Swiss-Webster mice  with 2 cm incision were divided into 3 groups. Negative control group were given Carboxymethyl cellulose 1% (CMC), positive control group were given feracrylum 1% (FER), and the eggshells group given domestic chicken eggshells powder (KT). Treatment and data mesurement was measured every day until  the 7th day. On the 7th  day the average length of the wound were analyzed and the skin tissue is taken for microscopic examination. Data analyzed using one-way ANOVA followed by Post Hoc Least Significant Difference (LSD) with p=0,05. On macroscopic examination, there were  highly significant differences between KT compared to CMC and highly significant differences between KT compared to FER. the average length of wound on the 7th day on KT 0,63 cm shorter than CMC 1,07 (p=0,000) and FER 0,81 cm (p=0,003). On microscopic examination, the degree of reepithelization on KT groups are severe, moderate for collagen density, fibroblasts density, subepithelial neutrophil density, degree of edema, and mild for angiogenesis. We conclude domestic chicken eggshells powder can accelerate wound healing. Key words: domestic chicken eggshells, incision wound, wound healing

Antioxidant Activity of Phyllanthus niruri Extract, Rutin and Quercetin

The Indonesian Biomedical Journal Vol 9, No 2 (2017)
Publisher : The Prodia Education and Research Institute (PERI)

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Abstract

BACKGROUND: Normal metabolism of oxygen and exogenous factors constantly generate free radicals which could be harmful to the human body. Human need antioxidants to provide protection against free radicals, thus plants are a good source of natural antioxidants. Phyllanthus niruri (P. niruri) has been known to possess several medicinal properties and contain numerous active phytochemical. In this research, we conducted phytochemical screening and antioxidant assay of P. niruri extract along with the compounds rutin and quercetin, which are flavonoids possessing medicinal properties. This study was conducted to determine P. niruri, rutin and quercetin as antioxidant.METHODS: In this study, qualitative phytochemical screening was performed to detect phenol, flavonoid, saponin, tannin, steroid/triterpenoid, terpenoid and alkaloid in P. niruri extract. Antioxidant analysis of P. niruri, rutin and quercetin was conducted using total measured phenolic content, 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,2’-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays.RESULTS:  The study revealed that P. niruri extract contained saponin, phenol, flavonoid and tannin based on phytochemical screening. In DPPH and ABTS assays quercetin possessed highest antioxidant activity with IC50 value of 0.55 and 1.17 μg/ml respectively. Meanwhile, P. niruri extract showed the highest FRAP activity which was 373.95 μM Fe(II)/μg extract. Rutin possessed the lowest antioxidant activity in all antioxidant assays.CONCLUSION: This study confirmed that P. niruri extract and quercetin have great potential as a natural antioxidant source.KEYWORDS: asntioxidant, phytochemical, Phyllanthus niruri, quercetin, rutin, free radical