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AKTIVITAS TABIR SURYA DARI FRAKSI FENOLIK BUAH SIRIH HUTAN (Piper miniatum. Bl) Budiarso, Leo Arifsandi; Suryanto, Edi; Sudewi, Sri
CHEMISTRY PROGRESS Vol 8, No 1 (2015)
Publisher : Sam Ratulangi University

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This study was intended to determine the sunscreen activity from the phenolic fraction of betel fruits. Betel fruits powder extracted with maseration method with ethanol 80%. Furthermore betel fruits extract liquid successively fractioned using petroleum eter, etyl acetate, butanol, ethanol and aquades. The phytochemical analysis of faction determined using total phenolic, flavonoid and condensed tannin content. The sunscreens activity was evaluated in sun protection factor (SPF) of fraction with spectrophotometry UV-vis.The best fractions were characterized functional group using infra red spectrophometer. The result showed that the butanol fraction have the highest total phenolic and condensed tannin content and petroleum eter fraction possessed highest total flavonoid content. Butanol fraction has the highest antioxidant activity than the other fraction. Aquades fraction (16.14) showed highest sun protection factor (SPF) value followed by petroleum eter, ethyl acetate, butanol and ethanol. SPF velue were 8.07; 6.93; 6.70; 5.38 dan 5.29.Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan potensi tabir surya dari fraksi buah sirih hutan. Serbuk buah sirih hutan diekstraksi dengan metode maserasi menggunakan pelarut etanol 80%. Ekstrak buah sirih hutan difraksinasi berturut turut dengan petroleum eter, etil asetat, butanol, etanol dan akuades. Analisis kandungan fitokimia fraksi-fraksi ditentukan berdasarkan total fenolik, flavonoid dan tannin terkondensasi. Aktivitas tabir surya pada fraksi-fraksi dievaluasi dengan analisis sun protection factor (SPF) menggunakan spektrofotometer UV-vis. Fraksi terbaik dikarakterisasi gugus fungsinya menggunakan spektrofotometer infra merah (FTIR). Hasil penelitian menunjukkan bahwa fraksi butanol memiliki kandungan total fenolik dan tanin terkondensasi tertinggi sedangkan kandungan flavonoid tertinggi terdapat pada fraksi butanol, petroleum eter dan etil asetat. Fraksi akuades memiliki aktivitas tabir surya tertinggi dibandingkan dengan fraksin diikuti fraksi petroleum eter, etil asetat, butanol dan etanol. Nilai SPF berturut-turut adalah 8,07; 6,93; 6,70; 5,38 dan 5,29.
KOMBINASI EKSTRAK BUAH MENGKUDU (Morinda citrifolia L.) DAN DAUN SIRSAK (Annona muricata L.) DALAM MENGHAMBAT BAKTERI Escherichia coli DAN Staphylococcus aureus Sudewi, Sri; Lolo, Widya Astuty
Kartika Jurnal Ilmiah Farmasi Vol 4, No 2 (2016)
Publisher : Universitas Jenderal Achmad Yani, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/kjif.v4i2.65

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ABSTRAK Penelitian ini tentang pemanfaatan bahan herbal yang sudah diketahui khasiat dan nilai terapinya dengan cara melakukan kombinasi dua macam bagian tanaman herbal  dimana  secara empiris masing-masing bagian tanaman tersebut sudah banyak dimanfaatkan oleh masyarakat luas dalam pengobatan penyakit. Penelitian ini dilakukan dengan tujuan untuk mengetahui daya hambat kombinasi ekstrak buah mengkudu dan daun sirsak pada konsentrasi efektif  dalam menghambat pertumbuhan bakteri E.coli dan S.aureus. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96 %.  Pengujian daya hambat dilakukan dengan metode difusi agar (difusi Kirby dan Bauer) dengan cara sumuran dan menggunakan ciprofloxacin sebagai kontrol positif serta aquadest sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa kombinasi ekstrak buah mengkudu dan daun sirsak pada konsentrasi 1000 µg menghasilkan zona hambat bakteri E.Coli dan S.Aureus sebesar 22, 625 dan 25,5 mm dengan kontrol positif sebesar 43,625 dan 46, 375 mm. Berdasarkan hasil penelitian, dapat dsimpulkan bahwa kombinasi ekstrak buah mengkudu dan daun sirsak dapat menghambat pertumbuhan bakteri E.coli dan S.aureus.           Kata kunci : daya hambat, ekstrak, bakteri ABSTRACT The research about utilization the known  herbal of its efficacy and therapy value by combinated two part of herbal plant while it has been used by society in disease medication empirically. This research was carried out for know inhibitory at combination of mengkudu fruits and sirsak leaves extract by effective concentration in inhibited growth E.coli and S. Aureus. Extraction was done by maceration with ethanol 96%. The test of inhibitory was carried out by agar difussion (Kirby and Bauer difussion) by pitting method and ciprofloxacin as positive control and aquadest as negative control. The result showed that combination of mengkudu fruits and sirsak leaves extract at 1000 µg  produced inhibitory zone of E. Coli and S. Aureus as 43,625 and 46, 375 mm. Based on the result,its concluded that combination of mengkudu fruits and sirsak leaves extract at 100% concentration could inhibited the E. coli and S. aureus growth.            Keywords : Inhibitory, extract, bactery
UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.) TERHADAP BAKTERI Klebsiella pneumonlae Melkianus, Brando; Fatimawali, Fatimawali; Sudewi, Sri
PHARMACON Vol 8, No 1 (2019): Pharmacon
Publisher : PHARMACON

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.) TERHADAP BAKTERI Klebsiella pneumonlaeBrando Melkianus1), Fatimawali1), Sri Sudewi1)1)PS. Framasi FMIPA Unsrat Manado, 95115ABSTRACTResearch has been carried out on the Antibacterial Activity Test of Mangosteen (Garcinia mangostana L.) Fruit Bark Extract against Klebsiella pneumoniae bacteria. This study aims to determine the antibacterial activity of mangosteen peel extract using the diffusion method with positive control of Ciprofloxacin and negative control of aquades and the test solution used by mangosteen peel extract. The result showed differences in extract concentrations of 25% (6.66 mm) categorized as medium, extract concentractions of 50% (8.83 mm) categorized as medium, extract concentractions of 75% (9.16 mm) categorized as medium, and extract concentractions of 100% (10.16 mm) categorized as strong in influencing the inhibition of the growth of Klebsiella pneumoniae bacteria. The result of this study also showed that mangosteen peel extract (Garcinia mangostana L.), has a broad-spectrum bioactive compound which means it can inhibit the growth of Gram negative bacteria.Keywords : Garcinia mangostana L, Klebsiella pneumoniae, CiprofloxacinABSTRAKTelah dilakukan penelitian tentang Uji Aktivitas Antibakteri Ekstrak Kulit Buah Manggis (Garcinia Mangostana L.) Terhadap Bakteri Klebsiella Pneumoniae. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kulit buah manggis menggunakan metode difusi agar dengan kontrol positif Ciprofloxacin dan kontrol negatif aquades serta larutan uji yang digunakan ekstrak kulit buah manggis. Hasil penelitian menunjukkan Perbedaan konsentrasi ekstrak 25% (6,66 mm) termasuk sedang, konsentrasi ekstrak 50% (8,83 mm) termasuk sedang, konsentrasi ekstrak 75% (9,16 mm) termasuk sedang, dan konsentrasi ekstrak 100% (10,16 mm) termasuk kuat dalam mempengaruhi penghambatan pertumbuhan bakteri Klebsiella Pneumoniae. Hasil penelitian ini juga menunjukan bahwa Ekstrak kulit buah manggis (Garcinia Mangostana L.), memiliki senyawa bioaktif dengan spektrum luas artinya dapat menghambat pertumbuhan bakteri Gram negatif.Kata kunci : My Garcinia Mangostana L, Klebsiella Pneunoniae, Ciprofloxacin 
AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADO Katiandagho, Lyone; Wewengkang, Defny S.; Sudewi, Sri
PHARMACON Vol 8, No 1 (2019): Pharmacon
Publisher : PHARMACON

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AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADOLyone Katiandagho1), Defny Silvia Wewengkang1), Sri Sudewi1)1)Program Studi Farmasi Fakultas MIPA UNSRAT Manado, 95115ABSTRACTSoft Coral Sinularia sp.has been shown to have antibacterial activity. This research aims to determine the antibacterial activity of marine organisms obtained from Manado bay. The method in this study is the diffusion method with positive control chloramphenicol and negative control of methanol in Escerichia coli and Sthaphylococcus aureus. The result showed that extracts, chloroform fraction and methanol-water fraction were obtained inhibiting category of Sthapylococcus aureus bacteria, but in the chloroform fraction and ethanol extract can inhibit Escerichia coli bacteria categorized as strong. With it can be conlueded that the extract and fraction of soft Sinularia sp. have bioactive compounds with a broth spectrum of antibacterial activity.Keywords: Sinularia sp., Chloramphenicol, Escerichia coli, Sthaphylococcus aureus, and antibacterial.ABSTRAKKarang lunak Sinularia sp. telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri pada organisme biota laut yang diperoleh dari teluk Manado. Metode dalam penelitian ini adalah metode difusi agar dengan kontrol positif kloramfenikol dan kontrol negatit metanol pada Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak, fraksi kloroform, dan fraksi metanol-air dapat menghambat bakteri Staphylococcus aereus dikategorikan sedang, namun pada fraksi kloroform dan ekstrak etanol dapat menghambat bakteri Escerichia coli dikategorikan kuat. Dengan demikian dapat disimpulkan bahwa ekstrak dan fraksi karang lunak Sinularia sp.memiliki senyawa bioaktif dengan spektrum yang luas terhadap aktivitas antibakteri.Kata kunci: Sinularia sp, Kloramfenikol, Escherichia coli, Staphylococcus aureus, dan antibakteri 
ISOLATION AND IDENTIFICATION OF FREE RADICALS SCAVENGER FROM Daucus carota L LEAVES Astuti, Puji; Wahyuono, Subagus; Sudewi, Sri
Majalah Obat Tradisional Vol 19, No 3 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1079.135 KB) | DOI: 10.22146/tradmedj.8371

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The presence of free radicals produced inside the cells is thought to cause a range of diseases such as degenerative diseases. Antioxidant properties of carrot root (Daucus carota L.) have been reported elsewhere to inhibit the formation of these free radicals, but the compounds responsible for antioxidant activity inside its leaves have not yet determined. In Indonesia, Daucus carota L. leaves are regarded as waste product after harvesting the root. This study was conducted to isolate and identify the compound having antioxidant activity from the leaves of D.carota. n-Hexane, ethyl acetate, and methanol extracts were examined for free radical scavenger activity by using DPPH method. The n-hexane extract showing free radical scavenger activity was solid-solvent partitioned, fractionated using vacuum liquid chromatography and tested for free radical scavenger activities. The active compound was isolated by using preparative thin layer chromatography and its purity was determined by TLC with three mobile phase systems and two dimensional TLC. Based on spectroscopy UV-Vis, IR as well as 1H-NMR (CDC13) data, the active compound was suggested to be xanthophyll derivate, lutein. The compound at concentration of 0.616, 1.025, and 2.05 ppm had antioxidant activities of 0.94 ± 0.05; 18.53 ± 0.15; and 49.07 ± 0.86, respectively.
EFEKTIVITAS INSEKTISIDA DALAM MENEKAN PERKEMBANGAN POPULASI DAN SERANGAN LIRIOMYZA, SP PADA TANAMAN BAWANG MERAH LOKAL PALU (Allium cepa L.x Wakegi Araki) DI DESA GUNTARANO KECAMATAN TANANTOVEA KABUPATEN DONGGALA Arfan, Arfan; Sudewi, Sri; Sataral, Mihwan; Sumarni, Sumarni; Rosiani, Vevi; Mumfahida, Mumfahida; Soar, Karmila
Jurnal Agrotech Vol 8 No 1 (2018)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/agrotech.v8i1.14

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This study aimed to examine the effectiveness of some insecticides on the population development and attack Liriomyza sp on local Palu onions. The research conducted from Februari to April 2017, at Guntarano village, Tanantovea Sub-District, Donggala District. The study used Randomized Block Design (RAK) consisting of four treatments, namely Po.Control; P1. Abamectin; P2. Azadiractin; P3. Dimohipo. Each treatment was repeated three times so that 12 treatments were obtained. The results showed that the insecticidal treatment had an effect on the development of the varied Liriomyza sp. Adult population at various plant age levels. Observations on plant age 5 and 6 of MST showed that abamectin insecticides were effective in suppressing the growth larvae populations of Liriomyza sp compared with the treatment of Azadiractin, Dimohipo and non-treated insecticides.
AKTIVITAS ANTIOKSIDAN DARI FRAKSI TONGKOL JAGUNG (Zea mays L.) YANG DIINDUKSI OLEH Fe2+ DAN CAHAYA UV-B Mawardi, Rizky H.; Suryanto, Edi; Sudewi, Sri
CHEMISTRY PROGRESS Vol 9, No 1 (2016)
Publisher : Sam Ratulangi University

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This research aims to study the anti UV-B activity from solvent fractions of corncobs. The study initiating by extracting the powder corn cobs using way reflux for two hours with 80% ethanol. Extract then partitioned using a series of solvent; n-hexane, ethyl acetate, butanol and distilled water. Then was determined the effect of fractions against lipid oxidation induced by UV-B light. The results showed that the ethyl acetate fraction showed the lowest content of conjugated diene followed fraction of butanol, n-hexane and distilled water. The content of conjugated diene was 21.89; 25.11; 42.94; and 46.08 mmol/kg, respectively. Based on this study, the fraction of ethyl acetate fraction could act as an antioxidant and anti UV-B better than the other fractionsPenelitian ini bertujuan untuk mempelajari aktivitas anti UV-B dari fraksi pelarut tongkol jagung. Penelitian dimulai dengan mengekstraksi serbuk tongkol jagung menggunakan cara refluks selama dua jam dengan pelarut etanol 80%. Ekstrak kemudian dipartisi berturut-turut menggunakan pelarut n-heksan, etil asetat, butanol, dan aquades. Kemudian ditentukan pengaruh fraksi terhadap oksidasi lipid yang diinduksi dengan cahaya UV-B. Hasilnya menunjukkan bahwa fraksi etil asetat memiliki kandungan diena terkonjugasi terendahdiikuti fraksi butanol, n-heksan dan aquades. Kandungan diena terkonjugasi berturut-turut adalah 21,89; 25,11; 42,94; dan 46,08 mmol/kg. Berdasarkan penelitian ini, dapat disimpulkan bahwa fraksi etil asetat dapat berperan sebagai antioksidan dan anti UV-B lebih baik dibandingkan dengan fraksi lainnya.
KARAKTERISASI DAN POTENSI ANTIOKSIDAN DARI KULIT LUAR UBI KAYU (Manihot utilissima) Karundeng, Glory; Suryanto, Edi; Sudewi, Sri
CHEMISTRY PROGRESS Vol 10, No 2 (2017)
Publisher : Sam Ratulangi University

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ABSTRACT Karundeng et al., 2017. Characterization and antioxidant potential of Cassava (Manihot utilissima) periderm  The aims of this research were to analyze the potential of ethanol extract of periderm of yellow cassava (PYC) and periderm of white cassava (PWC) in inhibiting in vitro oxidation of tissue homogenates of wistar rat and to identify phenolic compound contained in the extract. Inhibition effect was determined using 2-thiobarbituric acid reactive substances (TBARS) method by extracting liver, heart and brain to obtain homogenates and then evaluated with TBA. The functional group of phenolic compound in PYC and PWC was identified by infrared spectrophotometry. The results indicate that ethanol extract of PYC could inhibit oxidation by 37.13, 56.0 and 21.73% in liver, heart, and brain while PWC could inhibit oxidation by 52.63, 65.33, and 27.33% in the same organs. The identification of functional group with infrared spectrophotometer showed the presence of phenolic group with absorption peak 3399, 2926, 1251, 1611, 1517, and 1452 cm-1 in PYC and 3400, 2920, 1054, 1611, 1282, 1517, and 1452 cm-1 in PWC. The uv-vis spectrophotometer for PYC and PWC showed the peaks in 245 and 290 nm which indicate the presence of phenolic compound. Keywords :    Periderm, yellow cassava, white cassava, phenolic, homogenate, TBARS, infrared spectrophotometer, uv-vis spectrophotometer   ABSTRAK Karundeng, dkk., 2016. Karakterisasi dan potensi antioksidan dari kulit luar ubi kayu (Manihot utilissima)  Penelitian ini bertujuan untuk mengetahui potensi antioksidan ekstrak etanol periderm (kulit luar) umbi kuning (PUK) dan putih (PUP) dari ubi kayu dalam penghambatan reaksi oksidasi in vitro homogenat jaringan tikus putih galur wistar, serta identifikasi senyawa fenolik yang terkandung dalam ekstrak. Efek inhibisi ditentukan menggunakan metode 2-thiobarbituric acid reactive substances (TBARS) dimana jaringan hati, jantung dan otak diesktrak hingga didapatkan homogenat kemudian dilakukan pengujian inhibisi menggunakan TBA. Kandungan senyawa fenolik pada PUK dan PUP diidentifikasi gugus fungsionalnya menggunakan spektrofotometer inframerah dan spektrofotometer uv-vis. Ekstrak etanol periderm umbi kuning menunjukkan adanya potensi inhibisi sebesar 37,13, 56,0, dan 21,73% pada hati, jantung, dan otak sedangkan ubi putih menunjukkan potensi inhibisi sebesar 52,63, 65,33, dan 27,33%. Hasil identifikasi dengan spektrofotometer inframerah menunjukkan adanya gugus fenolik pada PUK dan PUP, ditandai dengan puncak pada 3399, 2926, 1251, 1611, 1517, dan 1452 cm-1 pada PUK serta 3400, 2920, 1054, 1611, 1282, 1517, dan 1452 cm-1 pada PUP. Adapun hasil serapan ekstrak PUK dan PUP pada spektrofotometer uv-vis yang menunjukkan puncak maksimum pada 245 dan 290 nm yang menunjukkan adanya kandungan senyawa fenolik. Kata kunci :         Periderm, ubi kuning, ubi putih, fenolik, homogenat, TBARS, spektrofotometri inframerah, spektrofotometri uv-vis
Kandungan Total Fenolik dan Aktivitas Antioksidan dari Fraksi Pelarut Sagu Baruk (Arenga microcarpha) Rondonuwu, Samuel D. J.; Suryanto, Edi; Sudewi, Sri
CHEMISTRY PROGRESS Vol 10, No 1 (2017)
Publisher : Sam Ratulangi University

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To evaluate the antioxidant from the solvent fraction of Sago Baruk (Arenga microcarpha) was the purpose of this study. These study initiated by extracting the powder of Sago Banrk by way of maceration for 4 days using 80% ethanol. Extract then partitioned using a series of solvent such as n-hexane ethyl acetate, buthanol, and distilled water, respectively. Then continued to determined the total phenolic content, total antioxidant. The results showed that the ethyl acetate fraction had the highest content of total phenolic followed by the fraction of buthanol, n-hexane and distilled water, with the value of total phenolic content as follow 81.22 (ethyl acetate);41.43 (buthanol);20.20 (n-hexane); and 14.39 mg I kg (aquades). Ethyl acetate fraction also have the highest total antioxidant activity compared to the other fractions. Based on this study, the ethyl acetate fraction was the best fraction with the ability of antioxidant. Penelitian ini bertujuan untuk mempelajari aktivitas antioksidan fraksi pelarut Sagu baruk. Penelitian dimulai dengan mengekstraksi serbuk Sagu baruk menggunakan cara maserasi selama 4 hari dengan pelarut etanol 80%. Ekstrak kemudian dipartisi berturut-turut menggunakan pelarut n-heksan, etil asetat, butanol, dan aquades. Kemudian ditentukan kandungan total fenolik, total aktivitas. Hasilnya menunjukkan bahwa fraksi etil asetat memiliki kandungan total fenolik tertinggi diikuti fraksi butanol, n-heksan dan aquades. Kandungan total fenolik berturut-turut adalah 81,22; 41,43; 20,20; dan 14,39 mg/kg. Fraksi etil asetat juga memiliki total aktivitas antioksidan tertinggi dibandingkan dengan fraksi lain. Berdasarkan penelitian ini, dapat disimpulkan bahwa fraksi etil asetat adalah fraksi terbaik yang dapat berperan sebagai antioksidan.
Sejarah Farmasi Islam dan Hasil Karya Tokoh-Tokohnya Sudewi, Sri; Nugraha, Sri Mardikani
Aqlam: Journal of Islam and Plurality Vol 2, No 1 (2017)
Publisher : IAIN Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30984/ajip.v2i1.511

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Pada zaman global ini, orang menganggap bahwa kemajuan ilmu farmasi berasal dari Barat. Padahal kemajuan yang dicapai Barat tersebut tidak lepas dari zaman sebelumnya, yakni dunia Islam. Para ilmuwan farmasi Muslim selain menguasai riset-riset ilmiah di bidang farmasi, mereka juga berhasil membuat komposisi, dosis, tata cara penggunaan, dan efek dari obat-obatan (baik obat sederhana maupun obat campuran). Masa kejayaan Islam merupakan masa di mana ilmu farmasi mencapai puncaknya. Tokoh-tokoh ilmu farmasi seperti Jabir bin Ibnu Hayyan, Ibnu Masawayh,  Al-Kindi, Sabur Ibnu Sahl, At-Tabari, Ar-Razi, Al-Zahrawi, Ibnu Sina, Al-Biruni, Al-Ghafiqi, Ibnu Zuhr, Ibnu Thufayl, Ibnu Rusyd, dan Ibnu Al-Baythar menjadi orang-orang di barisan terdepan bahkan beberapa karya mereka masih dijadikan rujukan dalam ilmu farmasi dan kedokteran hingga abad modern baik di negara Timur maupun di negara Barat.Kata kunci: Sejarah, farmasi Islam, hasil karya tokoh-tokoh farmasi Islam.In this global era, people see that development of the Pharmaceutical Science has come from the West. In fact, the progress achieved by the West is closely related to the previous time, that is Islamic world. The Muslim pharmaceutical scientists not only dominated scientific research in the pharmaceutical field but also determined compositions, dosages, ways of administering, and effect of drugs (both the simple and the combination). The glorious era of Islam is the peak of pharmaceutical sciences. Some of the figures known as seperti Jabir bin Ibnu Hayyan, Ibnu Masawayh,  Al-Kindi, Sabur Ibnu Sahl, At-Tabari, Ar-Razi, Al-Zahrawi, Ibnu Sina, Al-Biruni, Al-Ghafiqi, Ibnu Zuhr, Ibnu Thufayl, Ibnu Rusyd, dan Ibnu Al-Baythar were frontiers and even their research pieces are still being used as references in modern pharmaceutical and medical study both in the Eastern and Western countries.Keywords: History, Islamic pharmacy, research pieces of the Islamic pharmaceutical figures.