Moelyono Moektiwardoyo, Moelyono
Faculty of Pharmacy Universitas Padjadjaran Jl. Raya Bandung-Sumedang Km. 21 Jatinangor, West Java, Indonesia 45363

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Aktivitas Pro-Apoptosis Fraksi Air Daun Jawer Kotok (Plectranthus Scutellaroides) pada Ekor Larva Katak Rana Catesbeiana Stadium Metamorfosis Klimaks

JURNAL PHARMASCIENCE Vol 2, No 2 (2015): JURNAL PHARMASCIENCE
Publisher : JURNAL PHARMASCIENCE

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Abstract

ABSTRAK Daun Jawer kotok (Plectranthus scutellaroides), diketahui banyak mempunyai manfaat dalam pengobatan tradisional, salah satunya  sebagai antiinflamasi yang menjadi langkah awal pencarian anti kanker dari tumbuhan. Kanker adalah suatu penyakit yang diakibatkan terjadinya mutasi gen yang mengakibatkan kelainan pada kromosom. Penelitian ini bertujuan untuk mengetahui aktivitas pro-apoptosis fraksi polar daun jawer kotok (Plectranthus scutellaroides) terhadap ekor larva katak Rana catesbeiana saat stadium metamorfosis klimaks. Fraksi polar daun jawer kotok diperoleh melalui fraksinasi ekstrak etanol yang diperoleh dengan cara maserasi  dalam pelarut non polar, semi polar, dan pelarut polar. Metode pengujian aktivitas pro-apoptosis adalah dengan menghitung frekuensi sel apoptosis diantara sel nekrosis dan sel normal per seribu sel per ekor larva katak di bawah mikroskop. Jenis kematian sel ditentukan dengan pewarnaan Giemsa. Hasil pengujian menunjukkan bahwa fraksi polar daun jawer kotok dengan beberapa variasi   dosis mempengaruhi  frekuensi sel apoptosis jika dibandingkan dengan kontrol (a = 0,05). Frekuensi sel apoptosis tertinggi ditunjukkan oleh dosis 500 mg/kg BB yaitu sebesar 79,51%. Dari hasil penelitian dapat disimpulkan bahwa fraksi polar daun jawer kotok  menunjukkan  aktivitas pro-apoptosis pada ekor larva katak Rana catesbeiana stadium metamorfosis klimaks.Kata kunci :   Plectratus scutellaroides, apoptosis, Rana catesbeianaABSTRACTLeaves of jawer kotok (Plectranthus scutellaroides) has many benefits in tusing of traditional medicine, one among  them is antiinflammation which can be starting point of seeking and discovering of anticancer from plants. Cancer is one of disease due to gen mutation that effect anomaly of chromosom. The purpose of this experiment was to identify the pro-apoptotic activity of polar fraction of jawer kotok leaves on Rana catesbeiana tadpole tail in climax metamorphosis phase. Polar fraction was obtained through fractination of ethanolic extract which are made by maceration method in non polar, semi polar, and polar solvents.  The method use in this experiment was calculating the apoptotic cell frequency between necrotic cells and normal cells which were counted per one thousand of tadpole tails cells under microscope. The modes of cell death determined by Giemsa staining. The experiment showed that variation of dose of polar fraction of jawer kotok leaves is affecting  the frequency of apoptotic cell death compared with control (a = 0.05). Dose of 500 mg/kg body weight of water fraction of jawer kotok leaves gave the highest apoptotic cell frequency (79.51 %) between normal and necrotic cell. It can be concluded that polar fraction of jawer kotok leaves has pro-apoptotic activity on Rana catesbeiana tadpole tail in climax metamorphosis phase.Keywords: Plectranthus scutellaroides, apoptosis, Rana catesbeiana

The determination of quercetin in Plectranthus scutellarioides(L.) R.Br. leaves extract and its In SilicoStudy on Histamine H4 Receptor

INDONESIAN JOURNAL OF PHARMACY Vol 22 No 3, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Plectranthus  scutellarioides  (L.)  R.Br.,  or  jawer  kotok,  Family  Lamiaceae, grows  widely  in  Indonesia,  and  has  a  long  history  of  therapeutic  usage  in Indonesian traditional jamuto cure various diseases. The brownish purple leaves of  Plecranthus  contain  alkaloids,  saponin,  flavonoids,  tannin,  volatile  oils,  and quercetin  which  has  been  proven  to  exert  antiinflammatory  activity.  In  this research,  a  determination  of  quercetin  in  Plecranthus  leaves  extract  was performed and followed by a study of its interaction with histamine H4 receptor to  understand its  anti-inflammatory  activity.  The  dry  leaves  were  macerated by using  a  mixture  of  methanol  and  water  (1:1)  for  48  hours  and  the  solvent  was evaporated  at  low  temperature  (40-50oC).  Analysis  of  quercetin  in  the  extract was performed by using reversed-phase HPLC method LC-10AT VP (Shimadzu), Atlantis  Hilicsilica  C18  (Waters®)  150  mm  x  4.6  mm,  5  µm  as  stationary  phase and  a  mixture  of  acetonitrile,  phosphoric  acid,  and methanol  (40:50:10),  flow rate 0.8 mL/minute.  In silicostudy of quercetin with histamine H4 receptor was performed by using AutoDock Tools 3.0.5. Histamine H4 receptor (H4R) belongs to  G  protein-coupled  receptors  which  is  involved  in arthritis,  asthma,  and inflammations.  The  3D  structure  model  of  H4R  was  built  by  using  MODELLER 9v7.  Quercetin  contained  in  Plecranthus  leaves  extract  was  0.05  %.  This compound interacted with H4R viahydrogen bond formation with Lys158 (2.006 Å)  and  Glu182  (2.048  Å),  and  van  der  Waals  interaction  with  Trp90,  Leu91, Asp94, Tyr95, Phe168, Thr178, Ser179, Tyr319, Phe344, and Tyr340, therefore Plecranthus  leaves  extract  might  have  a  chance  to  be  used  as  histamine  H4 receptor inhibitor.Key  words  :   histamine  H4  receptor,  in  silico  study,  Plecranthus  leaves,  Plectranthus scutellarioides, quercetin

Aktivitas Antiproliferasi Ekstrak dan Fraksi Daun Kuhung-Kuhung (Crotalaria pallida Aiton) terhadap Sel Kanker Payudara MCF-7

Farmaka Vol 14, No 2 (2016): Farmaka
Publisher : Fakultas Farmasi Universitas Padjadjaran

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Abstract

Daun kuhung-kuhung (Crotalaria pallida Aiton) merupakan tumbuhan yang digunakan secara tradisional oleh etnis bolaang mongondow sebagai antikanker. Penelitian ini bertujuan untuk mengetahui aktivitas antiproliferasi dari ekstrak maupun fraksi daun kuhung-kuhung pada sel kanker payudara MCF-7. Pengujian aktivitas antiproliferasi dilakukan dengan metode WST assay menggunakan sel line MCF-7. Hasil penelitian ini menunjukkan bahwa fraksi etil asetat daun C. pallida Ait memiliki nilai IC50 terkecil yakni 29,67 µg/mL. Berdasarkan hasil tersebut, maka fraksi etil asetat daun kuhung-kuhung terbukti bersifat antiproliferasi dan bisa dijadikan dasar pengembangan tumbuhan antikanker

ARTIKEL ULASAN: BAWANG TIWAI (ELEUTHERINE AMERICANA MERR.), TANAMAN MULTIGUNA

Indonesian Journal of Applied Sciences Vol 4, No 2 (2014)
Publisher : Universitas Padjadjaran

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Abstract

AbstractTiwai onion (Eleutherine americana Merr.) is a plant that originated from tropical America and spread to Asia. This plant has been used for generations Kalimantan Dayak community as a medicinal plant. This article aims to review the content secondary metabolites, various uses, and pharmacological activities tiwai onion. Tiwai onion bulbs commonly used to treat various diseases, including cancer, diabetes, heart disease, inflammation, infection (bacteria, fungi, amoeba), hypertension, cholesterol, gout, bronchitis, and other diseases. The content secondary metabolites of this plant such as alkaloids, flavonoids, tannins,  glycosides, anthraquinones, naphtoquinones, steroids/triterpenoids, saponins, phenolic, polysaccharide, and azulen. Based onthe studies that have been done, this plant bulbs have various pharmacological activities, namely antioxidants, sunscreen activity, antiinflammation, antidiabetic, antiagregasi platelets, antibacterial, anticancer, antifungal, and antiamoeba, as well as having subacute toxicity in rat liver tissue.Keyword : Tiwai Onion, Eleutherine americana Merr

Senyawa Damara-20,24-dien-3-on yang beraktivitas Sitotoksis terhadap Sel Mcf-7 dari Kulit Batang Chisocheton Marcrophyllus (Meliaceae)

Indonesian Journal of Applied Sciences Vol 5, No 3 (2015)
Publisher : Universitas Padjadjaran

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Abstract

AbstrakSenyawa triterpenoid damaran, damara-20,24-dien-3-on telah diisolasi dari kulit batang Chisocheton Marcrophyllus (Meliaceae). Struktur kimia kedua senyawa tersebut telah ditetapkan berdasarkan data spektroskopi dan perbandingan data spektra yang diperoleh dari literatur. Senyawa damara-20,24-dien-3-on menunjukkan aktivitas sitotoksik terhadap sel kanker payudara manusia MCF-7 dengan nilai IC50 28 mM.Kata Kunci : aktivitas sitotoksik, sel MCF-7, triterpenoid damaran, Chisocheton Marcrophyllus,  MeliaceaeAbstractDammarane triterpenoid, damara-20,24-dien-3-on has been isolated from the bark of Chisocheton Marcrophyllus (Meliaceae). Chemical Structure of compound was dtermined on the basic of spektroscopic data and comparison with those spectra data previously reported. Compound damara-20,24-dien-3-on exhibited cytotoxic activity againts MCF-7 human breast cancer cell lines with IC50 value 28 mM respectively.Kata Kunci : cytotoxic activity, MCF-7 cell, dammarane triterpenoid, Chisocheton Marcrophyllus,  Meliaceae

ETNOFARMASI DAN ULASAN BIOPROSPEKTIF TUMBUHAN OBAT LIAR DALAM PENGOBATAN TRADISIONAL KAMPUNG ADAT CIKONDANG, KECAMATAN PANGALENGAN, KABUPATEN BANDUNG, JAWA BARAT

Farmaka Vol 15, No 1 (2017): Farmaka (Maret)
Publisher : Fakultas Farmasi Universitas Padjadjaran

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Abstract

Tumbuhan liar menyimpan potensi keragaman senyawa bioaktif. Penggunaan tumbuhan liar sebagai obat telah lama dilakukan oleh berbagai masyarakat etnis, termasuk masyarakat etnis Sunda yang tinggal di Kampung Adat Cikondang. Pendataan tumbuhan liar berpotensi obat dapat berkontribusi pada ditemukan berbagai obat baru. Telaah etnofarmasi dan bioprospeksi tumbuhan obat liar Kampung Adat Cikondang bertujuan untuk inventarisasi dan meninjau potensi penemuan obat baru. Penelitian ini dilakukan melalui metode participant observation dan wawancara kepada masyarakat yang dilanjutkan dengan analisis kuantitatif dari tumbuhan tersebut menggunakan use value (UV), relative frequency of citation (RFC), dan relative importance (RI). Tumbuhan dengan nilai tertinggi ditelaah dengan tinjauan pustaka untuk dilihat potensinya sebagai kandidat obat baru. Hasil menunjukkan terdapat 35 jenis dalam 22 suku tumbuhan liar yang digunakan sebagai obat oleh masyarakat Kampung Adat Cikondang. Hasil perhitungan menunjukkan bahwa Eupatorium inulifolium Kunth. (Asteraceae; ki rinyuh) adalah tumbuhan liar paling berpotensi dengan nilai UV, RFC, dan RI berturut-turut 0,5; 0,6; 0,33. Telaah pustaka menunjukkan bahwa E. inulifolium berpotensi untuk dikembangkan menjadi obat baru seperti kandidat obat kanker. Perlu telaah lebih lanjut mengenai informasi keamaan (toksisitas) penggunaan E. inulifolium sebagai obat.

Diabetes Patients Perceptions on Drugs Consultation Program by Pharmacists

Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

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Abstract

Drugs consultation by pharmacist is an important service to improve the quality of medication use in patients with type 2 diabetes mellitus (T2DM). Perception of patients regarding this service is very useful to boost pharmacists confidence to conduct such service. The purpose of this study is to provide an overview regarding interests and categories of drugs consultation needed by T2DM patients and to determine the effect of quality of drugs consultation on patients interest to participate in such program. Survey and interviews were conducted with T2DM patients in outpatient clinic, Dr. M. Salamun Air Force Hospital, Bandung during April-June 2013. One hundred patients were recruited for this study. Most of the patients consumed combination of oral antidiabetic and non-antidiabetic medication (71 %) and were given polypharmacy (45 %). The results showed that the information regarding drugs interactions, adverse drugs reaction (ADR), and drugs dosage were among the aspects that should be included in drugs consultation service by pharmacists. The quality of drug information service was associated with increased interest to participate in such program. These aspects should be considered in the implementation of drugs consultation program by pharmacists.Keywords: drugs consultation, T2DM, pharmacists

Anti-proliferative Activity of Crotalaria pallida Aiton on MCF-7 Breast Cancer Cells

Pharmacology and Clinical Pharmacy Research Vol 2, No 3
Publisher : Universitas Padjadjaran, Indonesia

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Abstract

 Crotalaria pallida Aiton (C. pallida Aiton) is empirically used as dietary supplement to treat cancer by the people of North Sulawesi. However, its scientific pharmacology activity has not been explored yet. Therefore, this study was conducted to evaluate anti-proliferative activity of C. pallida Aiton on MCF-7 breast cancer cells. The extraction of leaves and seeds were performed using ethanol, ethyl acetate, n-hexane, and water. Phytochemical screening was then performed to identify secondary metabolites in this extract. Anti-proliferative activity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results indicated that ethyl acetate fraction of C. pallida Aiton has the lowest IC50 (29,67). In conclusion, ethyl acetate fraction of C. pallida Aiton is potential to be developed as anti cancer agent.Keywords: Crotalaria pallida Aiton, WST assay, MCF-7 cell line

AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN ILER (Plectranthus scutellarioides (L.) R.Br.) PADA TIKUS PUTIH GALUR WISTAR DENGAN METODE INDUKSI ALOKSAN

Farmaka Vol 14, No 2 (2016): Farmaka
Publisher : Fakultas Farmasi Universitas Padjadjaran

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Abstract

Based on WHO data, in 2014  total of diabetic patient in Indonesia reach 9 million. In addition to synthetic drugs, traditional medicine widely used by diabetic patients. Research of several species from Plectranthus genus showed that Plectranthus amboinicus and P. esculentus have antidiabetic activity in rat. Therefore, it is necessary to do antidiabetic activity research and its effective dose of plants iler (Plectranthus scutellarioides (L.) R.Br) which derived from the same genus and has been used empirically as oral antidiabetic. Antidiabetic activity test conducted using white male Wistar rats which given diabetogen alloxan. Rat who had developed diabetes later given different dose of iler leaves ethanol extract and glibenclamide 0,5 mg/kgBB as a positive control. Blood glucose level was measured using amperometric method utilizing enzymatic reaction of glucose dehydrogenase which measured by glucometer. As a result, a dose of 200 mg / kg dose had the highest antidiabetic activity, followed by a dose of 300 mg / kg, the percentage decrease in relative blood glucose are 21,52% and 3,64% respectively, but a dose of 100 mg / kg didn’t have antidiabetic activity. Antidiabetic activity of 200 mg/kg dose of extract didn’t have any significant difference with glibenclamide, which has percentage decrease in relative blood glucose 21,35%.

Aktivitas Pro-Apoptosis Fraksi Air Daun Jawer Kotok (Plectranthus Scutellaroides) pada Ekor Larva Katak Rana Catesbeiana Stadium Metamorfosis Klimaks

Jurnal Pharmascience Vol 2, No 2 (2015): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

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Abstract

ABSTRAK Daun Jawer kotok (Plectranthus scutellaroides), diketahui banyak mempunyai manfaat dalam pengobatan tradisional, salah satunya  sebagai antiinflamasi yang menjadi langkah awal pencarian anti kanker dari tumbuhan. Kanker adalah suatu penyakit yang diakibatkan terjadinya mutasi gen yang mengakibatkan kelainan pada kromosom. Penelitian ini bertujuan untuk mengetahui aktivitas pro-apoptosis fraksi polar daun jawer kotok (Plectranthus scutellaroides) terhadap ekor larva katak Rana catesbeiana saat stadium metamorfosis klimaks. Fraksi polar daun jawer kotok diperoleh melalui fraksinasi ekstrak etanol yang diperoleh dengan cara maserasi  dalam pelarut non polar, semi polar, dan pelarut polar. Metode pengujian aktivitas pro-apoptosis adalah dengan menghitung frekuensi sel apoptosis diantara sel nekrosis dan sel normal per seribu sel per ekor larva katak di bawah mikroskop. Jenis kematian sel ditentukan dengan pewarnaan Giemsa. Hasil pengujian menunjukkan bahwa fraksi polar daun jawer kotok dengan beberapa variasi   dosis mempengaruhi  frekuensi sel apoptosis jika dibandingkan dengan kontrol (a = 0,05). Frekuensi sel apoptosis tertinggi ditunjukkan oleh dosis 500 mg/kg BB yaitu sebesar 79,51%. Dari hasil penelitian dapat disimpulkan bahwa fraksi polar daun jawer kotok  menunjukkan  aktivitas pro-apoptosis pada ekor larva katak Rana catesbeiana stadium metamorfosis klimaks.Kata kunci :   Plectratus scutellaroides, apoptosis, Rana catesbeianaABSTRACTLeaves of jawer kotok (Plectranthus scutellaroides) has many benefits in tusing of traditional medicine, one among  them is antiinflammation which can be starting point of seeking and discovering of anticancer from plants. Cancer is one of disease due to gen mutation that effect anomaly of chromosom. The purpose of this experiment was to identify the pro-apoptotic activity of polar fraction of jawer kotok leaves on Rana catesbeiana tadpole tail in climax metamorphosis phase. Polar fraction was obtained through fractination of ethanolic extract which are made by maceration method in non polar, semi polar, and polar solvents.  The method use in this experiment was calculating the apoptotic cell frequency between necrotic cells and normal cells which were counted per one thousand of tadpole tails cells under microscope. The modes of cell death determined by Giemsa staining. The experiment showed that variation of dose of polar fraction of jawer kotok leaves is affecting  the frequency of apoptotic cell death compared with control (a = 0.05). Dose of 500 mg/kg body weight of water fraction of jawer kotok leaves gave the highest apoptotic cell frequency (79.51 %) between normal and necrotic cell. It can be concluded that polar fraction of jawer kotok leaves has pro-apoptotic activity on Rana catesbeiana tadpole tail in climax metamorphosis phase. Keywords: Plectranthus scutellaroides, apoptosis, Rana catesbeiana