Dito Anggoro, Dito
Program Profesi PPDH, Fakultas Kedokteran Hewan, Universitas Gajah Mada (UGM)

Published : 3 Documents
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Effect of Ovaries T ransport Time to Quality Morphology of Cattle Oocytes In V itro Anggoro, Dito; Budiyanto, Agung
Jurnal Sain Veteriner Vol 33, No 1 (2015)
Publisher : Fakultas Kedokteran Hewan

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One of the most important factors in the production of in vitro embryos is oocyte quality . The excellent of oocyte quality will also produce a level of excellent cleavage and lastocyst. Transportation time from the abattoir (slaughterhouse) to the laboratory is one of the actors reported to influence the quality of the oocyte. The transportation time is right to produce oocytes with the best morphological quality has never been done for ovarian cattle in indonesia. This research was conducted to determine the effect of long time transportation to the quality of ovarian morphology of cattle oocytes collected in vitro. This research consisted of three stages: the collections of both ovarians and oocyte and evaluation of the oocytes quality as well. Ovarian collection done in slaughterhouse and the collected ovarians were then taken to the laboratory for oocyte collection process performed by the method of aspiration. Ovaries are grouped based on the time of transportation 2, 3, 4, and 5 hours, respectively . Obtained oocytes were evaluated to determine their qualities by classifying into quality A, B, C, and D. In this study , it was shown that there was a statistically significant differences between the longer transport time to the quality of the morphology of the bovine ovarian oocytes (P <0.05). This study suggests that a long period of transportation is related to the ovarian morphology of oocyte quality . The ovaries that were being subjected to transportation for 2 hours produces the percentage of oocytes with morphological quality A and B which were better compared to that of ovaries being subjected to transportation for more than 2 hours.
Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction Shanmuganathan, Lehgarubini; Anggoro, Dito; Wibowo, Michael Haryadi
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Abstract

Newcastle disease (ND) is a systemic, viral respiratory disease that is acute and easily transmitted which affects various types of poultry, especially chickens. Diagnosis of ND which generally involves virus isolation and subsequent identification with serological assays has limitations that needs more time. This research was aimed to detect Newcastle Disease virus (NDV) in chickens suspected with ND using the Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) technique. Nine chicken organ samples such as lien, trachea, and lungs were collected from chicken farms diagnosed with ND. The organ samples were processed and the targeted viral RNA was extracted using the RNA extraction kit. Genome amplification was performed with RT-PCR using specificprimers to target the F gene. Amplification results produced an amplicon product of 565 base pairs (bp). PCR product samples were then visualised using agar gel electrophoresis and viewed using the unified gel documentation system. Amplification results show nine samples positive for the DNA bands corresponding to the targeted band of the NDV F gene fragment. The results of this research confirm that the RT-PCR method is applicable for NDV detection from chicken organ samples.
Deteksi Molekuler Virus Infectious Bursal Disease (IBD) pada Samp l Bursa Fabrisius yang Diperoleh dari Ayam Terdiagnosa Penyakit IBD Wibowo, Michael Haryadi; Fadiar, Radhian; Anggoro, Dito; Artanto, Sidna; Amanu, Surya; Wahyuni, Agnesia Endang Tri Hastuti
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Kasus penyakit Infectious Bursal Disease (IBD) dewasa ini masih sering ditemukan pada peternakan ayam komersial baik layer maupun broiler di Indonesia. Diagnosis penyakit IBD sejauh ini mengandalkan lesi patologik spesifik dan kultur in ovo dengan mengamati lesi makroskopis embrio, serta diidentifikasi dengan uji agar gel presipitasi (AGP). Penelitian ini bertujuan menerapkan diagnosis dengan teknik reverse transcriptase polymerase chain reaction (RT-PCR) dari sampel Bursa Fabrisius (BF) sebagai konfirmasi pada kasus terdiagnosa IBD. Deteksi serologis virus IBD dengan uji AGP dengan sumber antigen chorioallantoic membrane (CAM) dan embrionya, untuk melihat potensinya sebagai sumber antigen uji AGP. Sampel BursaFabrisius sebanyak 5 yang diperoleh pada kasus terdiagnosa IBD, dikoleksi dari peternakan ayam komersial di Yogyakarta. Konfirmasi diagnosis dilakukan dengan metode RT-PCR. Sampel positip uji RT-PCR yang mengamplifikasi fragmen gen VP2. Isolasi virus IBD yang dilakukan kultur in ovo pada telur ayam berembrio (TAB) antibodi negatif terhadap virus IBD, berumur 11 hari. Desposisi materi inokulasi dilakukan pada (CAM), diinkubasi selama lima hari. Panen virus dilakukan dengan mengkoleksi membran korioalantois dan embrio, selanjutnya diamati lesi makroskopis yang timbul akibat infeksi virus IBD. Membran korioalantois dan embrioselanjutnya digerus dan diproses sebagai suspensi antigen yang digunakan dalam uji AGP. Hasil uji RT-PCR terhadap lima sampel Bursa Fabrisius yang dikoleksi dari peternakan ayam terdiagnosa penyakit IBD, tiga sampel menunjukkan hasil positif teramplifikasi fragmen gen VP-2 virus IBD dengan produk amplifikasi sebesar 440 bp, sedangkan dua sampel sisanya menunjukkan hasil negatif. Uji AGP dengan sumber antigen CAM menunjukkan hasil positip 2 dari 3 sampel yang diuji, sedangkan sumber antigen embrio menunjukkanhasil negatif. Berdasarkan data yang diperoleh dalam penelitian ini dapat disimpulkan bahwa uji RT-PCR dapat digunakan dalam mendeteksi virus IBD dari sampel BF terdiagnosa IBD. Uji AGP dengan sumber antigen CAM menunjukkan hasil lebih baik dari pada embrionya.