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IDENTIFICATION OF INFLUENZA VIRUSES IN HUMAN AND POULTRY IN THE AREA OF LARANGAN WET MARKET SIDOARJO-EAST JAVA, INDONESIA Frederika, Edith; Mareta, Aldise; Poetranto, Djoko; Wulandari, Laksmi; Setyoningrum, Retno Asih; Setyowati, Lucia Landia; Yudhawati, Resti; Soegiarto, Gatot; Yamaoka, Masaoki
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2006.144 KB)

Abstract

Background: Influenza is a viral infection that attacks the respiratory system (nose, throat, and lungs) that commonly known as “flu”. There are 3 types of influenza viruses, such as type A, type B, and type C. Influenza virus type A is the type of virus that can infect both human and animals, virus type B are normally found only in human, and Influenza virus type C can cause mild illness in human and not causing any epidemics or pandemics. Among these 3 types of influenza viruses, only influenza A viruses infect birds, particularly wild bird that are the natural host for all subtypes of influenza A virus. Generally, those wild birds do not get sick when they are infected with influenza virus, unlike chickens or ducks which may die from avian influenza. Aim: In this study, we are identifying the influenza viruses among poultry in Larangan wet market. Method: Around 500 kinds of poultry were examined from cloacal swab. Result: Those samples were restrained with symptoms of suspected H5. The people who worked as the poultry-traders intact with the animal everyday were also examined, by taking nasopharyngeal swab and blood serum. Conclusion: Identification of influenza viruses was obtained to define the type and subtype of influenza virus by PCR.
IDENTIFICATION OF INFLUENZA VIRUSES IN HUMAN AND POULTRY IN THE AREA OF LARANGAN WET MARKET SIDOARJO-EAST JAVA, INDONESIA Frederika, Edith; Mareta, Aldise; Poetranto, Djoko; Wulandari, Laksmi; Setyoningrum, Retno Asih; Setyowati, Lucia Landia; Yudhawati, Resti; Soegiarto, Gatot; Yamaoka, Masaoki
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2006.144 KB) | DOI: 10.20473/ijtid.v4i4.230

Abstract

Background: Influenza is a viral infection that attacks the respiratory system (nose, throat, and lungs) that commonly known as “flu”. There are 3 types of influenza viruses, such as type A, type B, and type C. Influenza virus type A is the type of virus that can infect both human and animals, virus type B are normally found only in human, and Influenza virus type C can cause mild illness in human and not causing any epidemics or pandemics. Among these 3 types of influenza viruses, only influenza A viruses infect birds, particularly wild bird that are the natural host for all subtypes of influenza A virus. Generally, those wild birds do not get sick when they are infected with influenza virus, unlike chickens or ducks which may die from avian influenza. Aim: In this study, we are identifying the influenza viruses among poultry in Larangan wet market. Method: Around 500 kinds of poultry were examined from cloacal swab. Result: Those samples were restrained with symptoms of suspected H5. The people who worked as the poultry-traders intact with the animal everyday were also examined, by taking nasopharyngeal swab and blood serum. Conclusion: Identification of influenza viruses was obtained to define the type and subtype of influenza virus by PCR.
LYMPHOCYTE-T TYPE TH1 AND TH2 ACTIVITY DIFFERENCE OF LUNG TISSUE ON Heligmosomoides polygyrus NEMATODE AND Mycobacterium tuberculosis SEQUENTIAL CO-INFECTION Wulandari, Laksmi; Amin, Muhammad; Soedarto, Soedarto; Soegiarto, Gatot
Folia Medica Indonesiana Vol 53, No 2 (2017): JUNE 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v53i2.6356

Abstract

Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis that are often associated with uneffectiveness of the BCG vaccine and the high worm infection. The objective of this study was to determine the differences in the activity of Limfosit T type Th1 (IFN-g) and Th2 (IL-4) in lung tissue on Heligmosomoides polygyrus nematode and Mycobacterium tuberculosis sequential co-infection. This research using 49 mice were divide into 7 groups treated with infection by Mycobacterium tuberculose inhaled and Heligmosomoides polygyrus orally within 8 and 16 weeks. The levels of IFN-g in peripheral blood serum (89.929 + 3.533 pg/mL) resembles the pattern of the percentage of lymphocytes T CD4+ Th1 in lung tissue (3.246 + 0.519%) and peripheral blood (4.950 + 0.237%), while the levels IL-4 in the peripheral blood serum (20.782 + 4.043%) resembles the pattern of the percentage of lymphocytes T CD4+ Th2 in intestinal tissue (1.048 + 0.359%) and peripheral blood (1.196 + 0.557%). In conclusion, there is difference in the activity of lymphocytes T type Th1 and Th2 but it does not affect the immune response to Mycobacterium tuberculosis infection.
COMPARISON OF CHEMOTHERAPY RESPONSE AND ADVERSE EFFECTS OF DOUBLE-PLATINUM PLUS EGFR-TKI VERSUS DOUBLE-PLATINUM ALONE ON NSLCLC PATIENTS WITH DISEASE PROGRESSION AND EGFR-TKI TREATMENT Wulandari, Laksmi; Wiriansya, Edward Pandu
Folia Medica Indonesiana Vol 53, No 4 (2017): December 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v53i4.7161

Abstract

EGFR-TKI is the first-line therapy for EGFR-mutant patients. Nevertheless, patients will have disease progression (median PFS 10 – 12 months) due to resistance. The treatment options are still limited in developing countries for such cases, thus double-platinum chemotherapy is the next option. Although IMPRESS study reported no difference in terms of PFS and OS between double-platinum alone and double-platinum plus EGFR-TKI, several local studies reported benefit of continuing EGFR-TKI in combination with double-platinum chemotherapy (treatment beyond progression). This study aimed to compare chemotherapy effects of double-platinum plus EGFR-TKI versus double-platinum alone on patients with NSCLC progression after EGFR-TKI treatment. This was an analytical descriptive study using prospective cohort design, involving 30 patients with disease progression following EGFR-TKI treatment that met inclusion criteria in Dr. Soetomo Hospital. Subjects were divided into two groups: arm A (double-platinum plus EGFR-TKI) and arm B (double-platinum alone). Subjects were observed until 4 cycles of double-platinum chemotherapy. Subjective response (body weight and EQ5D questionnaire) was analyzed, chest CT scans were evaluated using RECIST criteria, and adverse effects were monitored. This study was conducted in accordance with GCP principles and has received ethics certificate from Dr. Soetomo Hospital ethics committee (No. 08/Panke.KKE/I/2017). The results showed that subject characteristics between two arms were insignificantly different (p=0.05). The most common EGFR mutation was exon 21 (50% on arm A and 60% on arm B). Chi square was tested on subjective response parameter (EQ5D (p=0.483)). T2 free sample was tested on semi-subjective parameter (body weight (p=1.00)). Comparison test on both groups after cycle 2 and 4 showed p value=0.05. Statistical test on adverse effect between both groups showed p value=0.526. As a conclusion, there was no significant difference between double-platinum and double-platinum plus EGFR-TKI on patients who had disease progression following EGFR-TKI treatment.
Terapi ARV pada Penderita Ko-Infeksi TB-HIV Ajmala, Indana Eva; Wulandari, Laksmi
Jurnal Respirasi Vol 1, No 1 (2015): Januari 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.585 KB) | DOI: 10.20473/jr.v1-I.1.2015.22-28

Abstract

TB and HIV have a very close relationship since the development of AIDS. Through a significant reduction in cellular immunity, HIV affects the pathogenesis of tuberculosis, thereby increasing the risk of TB in HIV co-infected individuals. In 2006, there were an estimated 9.2 million new TB cases worldwide, there were 710.00 in patients with HIV and 500,000 cases with MDR-TB. Sensitivity to TB associated with cytokine production by T lymphocytes (IFN gamma and TNF are like alpha). During HIV infection, IFN gamma production declined dramatically in line with the decrease in CD4 T lymphocytes This leads to an increased risk of developing reactivation or reinfection Mycobacterium tuberculosis. Clinical symptoms of pulmonary TB in people living with HIV are often non-specific. Clinical symptoms often found are fever and significant weight loss. The other symptoms usually associated with extrapulmonary TB. Antiretrovirals are drugs that inhibit HIV replication. The main priority in patients co-infected with TB-HIV is a start of TB therapy, followed by cotrimoxazole and ARV. ARV treatment recommendation on co-infection tuberculosis is starting ARV therapy to all people living with HIV with active TB, regardless of CD4 cell count. Antiretroviral therapy start as soon as possible after TB treatment can be tolerated, as soon as 2 weeks and no more than 8 weeks. Regimen set by WHO for first-line regimen containing two nucleoside reverse transcriptase inhibitors (NRTIs) plus one non-nucleoside reverse transcriptase inhibitors (NNRTIs). In the co-infection of TB-HIV nucleoside was elected WHO recommended Zidovudine (AZT) or tenofovir disoproxil fumarate (TDF), in combination with lamivudine (3TC) or emricitabine (FTC). For NNRTI, WHO recommends efavirenz (EFV) or nevirapine (NVP).
Problem Penegakkan Diagnostik Pasien dengan Massa di Paru Wulandari, Laksmi; Faot, Nikson Eduard
Jurnal Respirasi Vol 3, No 2 (2017): Mei 2017
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (977.856 KB) | DOI: 10.20473/jr.v3-I.2.2017.41-46

Abstract

Background: Lung cancer are divided into 2 groups; i.e. Small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). About 30% of NSCLC is squamus cell carcinoma and the other is adeno carcinoma. Late diagnosis makes such a high mortality rate. Early diagnosis plays a very important role in the management of therapy. Case: Patients complain of chronic cough more than 6 months, decreased appetite and weight loss. Chest X-ray and CT scan show a density of round shape mass, with firm border and smooth regular edge, also lympnodes enlargement (T2N2Mx) stage IIb. Appearance of lung mass is benign according to clinically and radiologically. FNAB-CT Guiding results are difference each time examination. The first result is squamous cell carcinoma but the last result is teratoma. Discussion: Based on the multidisciplinary discussion on Tumor Board Meeting, the diagnosis was decide as Squamous cell Carcinoma. The choice management of stage IIb lung cancer is surgery (Lobectomy). Histopathology finding post surgery is a Squamus Cell Carcinoma. Patients were then given adjuvant chemotherapy for 4 cycles with Platinum Base regimen paxuscarboplatin with the aim of clearing micrometastase that may still be left behind. The complete respons of platinum based chemotherapy following surgery in early stage of squamous cell carcinoma. Conclusion: Difficulties of lung cancer diagnostic are still the problem in the management of lung mass. Difference of diagnostic makes difference treatment choice. Multidiscipline discussion is needed to decide the diagnostic and treatment judgment.
PENINGKATAN EKSPRESI LAMININ NAMUN TIDAK VE-CADHERIN PADA SAWAR DARAH OTAK SETELAH INFEKSI Mycobacterium tuberculosis INTRAPULMONALIS Widayati, Aris; Wulandari, Laksmi; Riawan, Wibi
Majalah Kesehatan FKUB Vol 5, No 3 (2018): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

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Abstract

Tuberkulosis merupakan  masalah kesehatan utama di dunia. Pada tahun 2016, WHO menemukan angka kejadian TB kurang lebih 10,4 juta, dan untuk Indonesia dilaporkan sebesar 156.723 kasus. Meskipun penyebaran Mycobacterium tuberculosis di susunan saraf pusat tercatat hanya 1%, namun memiliki tingkat kecacatan dan kematian yang tinggi, sehingga menuntut adanya tatalaksana yang efektif untuk mengatasinya. VE-chaderin dan laminin merupakan protein adhesin yang berfungsi mengendalikan permeabilitas pembuluh darah dan mempertahankan integritas blood brain barrier, sehingga kedua protein adhesin tersebut dapat menjadi salah satu target terapi tuberkulosis otak. Penelitian ini bertujuan untuk mengetahui efek paparan M. tuberculosis secara inhalasi terhadap ekspresi laminin dan VE-chaderin pada sel endotel blood brain barrier. Penelitian ini menggunakan mencit Balb/c  (Mus musculus) yang diinfeksi oleh M.tuberculosis strain H37Rv secara inhalasi. Jaringan otak diperiksa menggunakan metode imunohistokimia dengan  antibodi mt-38, antibodi VE-chaderin dan laminin. Hasil penelitian menunjukkan adanya invasi M. tuberculosis pada  mikroglia jaringan otak mencit, diiketahui juga adanya peningkatan ekspresi laminin, sedangkan VE-chaderin tidak menunjukkan adanya perubahan. Proses masuknya M. tuberculosis ke otak diduga terjadi melalui proses diapedesis atau melalui peningkatan ekspresi laminin tanpa perubahan pada VE-chaderin dan reseptor laminin diduga sebagai tempat berikatan yang memungkinkan bakteri tersebut masuk ke jaringan otak. 
Tumor Mediastinum Anterior ( Yolk Sac Tumor ) pada Seorang Laki-Laki Dewasa Muda: Sebuah Kasus yang Jarang Risnawati, Risnawati; Wulandari, Laksmi
Jurnal Respirasi Vol 2, No 2 (2016): Mei 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (944.996 KB) | DOI: 10.20473/jr.v2-I.2.2016.45-51

Abstract

Background: Primary mediastinal yolk sac tumor is an extremely rare and highly malignant tumors occuring in children and young adult. They are more common in men. The most common symptoms on ptesentation were dyspnea, chest pain, cought, fever, night sweat, or weight loss. Primary mediastinal tumor are considered to have poor prognosis. Case: A 18- year-old man who presented with shorthness of breath, chest pain, fever, night sweat, and generalized weakness. He had no significant surgical, familiy or social history. The chest X-Ray and computed tomographic scan of the chest showed a large anterior mediasti nal mass. The serum level of alpha- fetoprotein and β-HCG was elevated. The histological examination revealed the finding of yolk sac tumor. This supported the diagnosis of yolk sac tumor. The patient received a combination chemotherapy consisting of cisplatin, etoposide and bleomycin every 3 weeks for total of 4 cycles. Conclusions: Primary mediastinal yolk sac tumor is a rare tumor. The diagnosis should be made not only by morphological studies but the also the patients age and the elevation of serum alpha-fetoprotein. In spite of modern chemotherapy, the prognosis of mediastinal yolk sac tumor remains poor.
Lung Cancer Patients’ Profile in Dr Soetomo General Hospital Surabaya 2016 - 2017: Newest Report Aini, Sita Ro'yul; Wulandari, Laksmi; Andajani, Susilowati
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol 10, No 1 (2019): VOL X, NO.1 (2019) : Journal of Aesculap Medical Science
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.384 KB)

Abstract

A B S T R A C TMethods: This research was a descriptive observational study. The samples were 240 patients of lung cancer in Poly Oncology Dr. Soetomo General Hospital Surabaya 2016-2017. Sampling technique was non-random sampling (consecutive sampling). The variables were: age, sex, smoking habits, type of lung cancer, type of mutation, stage, symptoms, Vena Cava Superior syndrome, and therapy. The data analyzed were analyzed descriptively.Results: Patients mostly at age 51-55 and 56-60 years old (18% each). Ratio men and women are 1,7:1. In all type of lung cancer, men are dominant than women and mostly patients is active smoker. Ephidermal Growth Factor Receptor (EGFR) mutation found in adenosquamous (75%) and adenocarcinoma (65%). Most patients diagnosed at advance stage. Most symptoms are cough (70.4%), dyspnoea (50%), and chest pain (49.2%). Vena Cava Superior Syndrome (VCSS) found in adenocarcinoma (75%) and squamous cell carcinoma (25%). In adenocarcinoma and adenosquamous, patients commonly got targeted therapy.Conclusion: Lung cancer patients: 1) Men tend to be exposed to younger than women, 2) ratio men and women is 1.7:1 and most type in lung cancer is adenocarcinoma 3) mostly patients is active smoker, 4) EGFR mutation tent to be in women with adenocarcinoma, 5) most stage is stage IV, 6) most symptoms is cough, 7) Vena Cava Superior Syndrome is in adenocarcinoma (75%) and carcinoma cell squamous (25%), 8) patients’ therapy mostly with targeted therapy followed by chemotherapy.