Suryani Dyah Astuti, Suryani Dyah
Department of Physics, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia

Published : 11 Documents
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APPLICATION OF NEURAL NETWORKS ON BLOOD SERUM IMAGE FOR EARLY DETECTION OF TYPHUS Purnamasari, Betty; Arisgraha, Franky; Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.71 KB)

Abstract

Background: Typhus is a disease caused by Salmonella typhi, Salmonella paratyphi A Salmonella parathypi B, dan Salmonella paratyphi C bacteria that attacks digestive tract and caused infection in small intestine. The common test that performed in the laboratory is widal test. The result reading of the widal test still processed manually with looking the turbidity caused by the agglutination. Aim: The research was made to decrease human error by creating a program based on artificial neural network (ANN) with learning vector quantization (LVQ) method. Method: Input of this program is image of blood serum that has reacted with widal reagen. Image procesing start with grayscaling, filtering, and thresholding. Result: Output of this program is divided into two classes, normal and typhus detected. Conclusion: From this experiment result that using 24 testing data, gives the accuracy of this program 95.833% with 1 error result from 24 testing data.
The Photodynamic Effect of LED-Magnetic Exposure to Photoinactivation of Aerobic Photosyntetic Bacteria Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 1 (2014)
Publisher : Institute of Topical Disease

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Abstract

All photosynthetic bacteria have a major pigment of bacteriochlorophyl and accessor pigment e.g. the carotenoids, which both have an important role in photosynthesis process. This study aim to explore the exogenous organic photosensitizer from photosyntetic bacteria for photodynamic therapy application. This study is an experimental research aiming to test the potential illumination of LED with wavelength 409, 430, 528 and 629 nm, and power optimization and time exposure LED-magnetic for optimum photo activation Rhodococcus growth. The reseach design use a factorial completely randomized design with factor of power and exposure time. The number of bacterial colonies grown measure using of Total Plate Count (TPC) methods. The result of anova test shows that irradiation treatment with LED 409 nm, 430 nm, 528 nm and 629 nm significantly affects on bacterial colony growth. LED 409 nm exposure has the greatest potential to boost the growth of bacterial colonies by 77%. LED exposure and the addition of 1.8 mT magnetic fieldincreases bacterial colony growth by 98%. Results of optimization of LED and magnetic fields show power 46 mW and a 40 minute (energy dose 110 J/cm2) optimum growth of bacterial colonies increase by 184%. So LED and magnetic illumination has potentially increased the viability of an aerob photosyntetic bacteria colonies.
OPTIMATION OF 48 kHz ULTRASONIC WAVE DOSE FOR THE INACTIVATION OF Salmonella typhi Lestari, Dwi May; Prijo, Tri Anggono; Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 4 (2014)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.114 KB)

Abstract

This study was aimed to determine the effect of ultrasonic dose exposure which could decrease the viability of Salmonella typhi by using the variation of exposure time (15, 20, 25, and 30 minutes) and volume of bacterial suspension (2, 4, 6, and 8 ml) at constant power. The sample used was Salmonella typhi. Ultrasonic wave transmitter was a piezoelectric tweeter with 0,191 watts of power and 48 kHz frequency generated by the signal generator. Piezoelectric tweeter was a kind of transducer which converted electrical energy into ultrasonic energy. This research was an experimental laboratory with a completely randomized design. The decrease of bacterial percentage was calculated by using TPC (Total Plate Count). Data were analyzed by using One Way Anova. The results showed that the variation of exposure time and volume of bacterial suspension gave significant effect on the percentage of Salmonella typhi kill. The most optimal of ultrasonic dose exposure to kill Salmonella typhi was 281.87 J/ml with 100% bacterial kill.
OPTIMATION OF 48 kHz ULTRASONIC WAVE DOSE FOR THE INACTIVATION OF Salmonella typhi Lestari, Dwi May; Prijo, Tri Anggono; Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 4 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.592 KB) | DOI: 10.20473/ijtid.v5i4.2006

Abstract

This study was aimed to determine the effect of ultrasonic dose exposure which could decrease the viability of Salmonella typhi by using the variation of exposure time (15, 20, 25, and 30 minutes) and volume of bacterial suspension (2, 4, 6, and 8 ml) at constant power. The sample used was Salmonella typhi. Ultrasonic wave transmitter was a piezoelectric tweeter with 0,191 watts of power and 48 kHz frequency generated by the signal generator. Piezoelectric tweeter was a kind of transducer which converted electrical energy into ultrasonic energy. This research was an experimental laboratory with a completely randomized design. The decrease of bacterial percentage was calculated by using TPC (Total Plate Count). Data were analyzed by using One Way Anova. The results showed that the variation of exposure time and volume of bacterial suspension gave significant effect on the percentage of Salmonella typhi kill. The most optimal of ultrasonic dose exposure to kill Salmonella typhi was 281.87 J/ml with 100% bacterial kill.
APPLICATION OF NEURAL NETWORKS ON BLOOD SERUM IMAGE FOR EARLY DETECTION OF TYPHUS Purnamasari, Betty; Arisgraha, Franky; Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.71 KB) | DOI: 10.20473/ijtid.v4i4.234

Abstract

Background: Typhus is a disease caused by Salmonella typhi, Salmonella paratyphi A Salmonella parathypi B, dan Salmonella paratyphi C bacteria that attacks digestive tract and caused infection in small intestine. The common test that performed in the laboratory is widal test. The result reading of the widal test still processed manually with looking the turbidity caused by the agglutination. Aim: The research was made to decrease human error by creating a program based on artificial neural network (ANN) with learning vector quantization (LVQ) method. Method: Input of this program is image of blood serum that has reacted with widal reagen. Image procesing start with grayscaling, filtering, and thresholding. Result: Output of this program is divided into two classes, normal and typhus detected. Conclusion: From this experiment result that using 24 testing data, gives the accuracy of this program 95.833% with 1 error result from 24 testing data.
The Photodynamic Effect of LED-Magnetic Exposure to Photoinactivation of Aerobic Photosyntetic Bacteria Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 1 (2014)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (818.491 KB) | DOI: 10.20473/ijtid.v5i1.208

Abstract

All photosynthetic bacteria have a major pigment of bacteriochlorophyl and accessor pigment e.g. the carotenoids, which both have an important role in photosynthesis process. This study aim to explore the exogenous organic photosensitizer from photosyntetic bacteria for photodynamic therapy application. This study is an experimental research aiming to test the potential illumination of LED with wavelength 409, 430, 528 and 629 nm, and power optimization and time exposure LED-magnetic for optimum photo activation Rhodococcus growth. The reseach design use a factorial completely randomized design with factor of power and exposure time. The number of bacterial colonies grown measure using of Total Plate Count (TPC) methods. The result of anova test shows that irradiation treatment with LED 409 nm, 430 nm, 528 nm and 629 nm significantly affects on bacterial colony growth. LED 409 nm exposure has the greatest potential to boost the growth of bacterial colonies by 77%. LED exposure and the addition of 1.8 mT magnetic fieldincreases bacterial colony growth by 98%. Results of optimization of LED and magnetic fields show power 46 mW and a 40 minute (energy dose 110 J/cm2) optimum growth of bacterial colonies increase by 184%. So LED and magnetic illumination has potentially increased the viability of an aerob photosyntetic bacteria colonies.
Fotodinamik Inaktivasi Laser Diode Pada Bakteri Staphylococcus aureus Dengan Endogen Porfirin Secara In Vitro Astuti, Suryani Dyah; Widya, Indira Wastu; Apsari, Retna
Jurnal Biosains Pascasarjana Vol 19, No 3 (2017)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bsn.v19i3.Y2017.8763

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh fotodinamik inaktivasi pemaparan dosis laser diode biru 405nm terhadap kematian bakteri staphylococcus aureus secara in vitro dengan menggunakan endogen fotosensitizer guna mengetahui dosis yang optimum untuk fotoinaktivasi bakteri. Dosis energi yang dipaparkan berkisar antara 31,740 - 71,415. Pemaparan laser menghasilkan persentase kematian bakteri tertinggi sebesar 55,22% pada dosis energi 63,480  dan mengalami penurunan pesentase kematian pada dosis diatas 63,480. Berdasarkan hasil penelitian tersebut, dapat disimpulkan bahwa pemaparan dosis energy laser diode biru berpotensi untuk menginaktivasi bakteri staphylococcus aureus sehingga dapat digunakan sebagai kandidat terapi fotodinamik.Kata kunci - Fotoinaktivasi, Laser dioda, Endogen porfirin, Staphylococcus aureus,
Optimalisasi Parameter Bandwidth dan Time Echo untuk Mengurangi Susceptibility Artifacts dan Chemical Shift pada MRI Astuti, Suryani Dyah; Astutik, Nur Vita Indri; Muzamil, Akhmad
Jurnal Biosains Pascasarjana Vol 19, No 3 (2017)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bsn.v19i3.Y2017.8839

Abstract

AbstrakTelah di lakukan penelitian yang berjudul Optimalisasi Parameter Bandwidth dan Time Echo untuk Mengurangi Susceptibility  Artifacts dan Chemical Shift. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh Bandwidth (BW) dan Time Echo (TE) pada MRI Shoulder serta mengetahui nilai variasi Bandwidth dan Time Echo yang optimal untuk mengurangi Susceptibility  Artifacts dan Chemical Shift. Penelitian ini di lakukan di RSU Haji Surabaya  dengan Pesawat MRI 1,5 Tesla,  parameter Bandwidth 170 Hz/Px, 190 Hz/Px dan 210 Hz/Px dengan  variasi TE 16s dan 20s pada pembobotan T2 Medic potongan axial terhadap 4 pasien. Analisa data dilakukan secara kuantitatif dengan menggunakan metode Region of Interest (ROI) pada komputer MRI kemudian dilakukan analisis Signal to Noise Ratio (SNR) dan Contrast to Noise Ratio (CNR) serta analisis terhadap Susceptibility  Artifacts dan Chemical Shift. Variasi Time Echo dan bandwidth berpengaruh signifikan terhadap nilai SNR dan CNR pada hasil citra T2 Axial Gradient Echo MRI Shoulder. Pengaruh kedua variasi tersebut adalah semakin kecil nilai TE dan BW didapatkan SNR yang meningkat. Jika TE diperbesar maka didapatkan CNR yang meningkat. Kualitas citra optimal yang dapat menghasilkan keadaan patologis ditentukan dengan meningkatnya nilai CNR dan nilai artefak yang menurun. Sehingga Pada penelitian ini kombinasi antara 2 variabel tersebut dapat menentukan citra optimal pada TE 20ms dan BW 210 Hz/Px. Kata Kunci: Bandwidth, TE, Susceptibility Artifacts, Chemical Shift, MRI Shoulder
Optimalisasi Citra Axial Sequence T2 Gradient Echo Dengan Variasi Bandwidth Dan Time Echo Pada MRI Shoulder Untuk Mengurangi Susceptibility Artifacts Dan Chemical Shift Muzamil, Akhmad; Indri, Nur Vita; Astuti, Suryani Dyah; Prijo, Tri Anggono
Journal of Health Vol 5 No 2 (2018): Journal of Health - July 2018
Publisher : LPPM STIKES Guna Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1224.753 KB) | DOI: 10.30590/vol5-no2-p40-49

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh Bandwidth (BW) dan Time Echo (TE) pada MRI Shoulder serta mengetahui nilai variasi Bandwidth dan Time Echo yang optimal untuk mengurangi Susceptibility Artifacts dan Chemical Shift. Penelitian ini di lakukan di Rumah Sakit Umum Haji Surabaya dengan menggunakan Pesawat MRI 1,5 Tesla. Penelitian ini  menggunakan varaisi Bandwidth 170 Hz/Px, 190 Hz/Px dan 210 Hz/Px serta menggunakan variasi TE 16 ms dan 20 ms pada pembobotan T2 Medic potongan axial terhadap 4 pasien. Analisa data dilakukan secara kuantitatif dengan menggunakan metode Region of Interest (ROI) pada komputer MRI kemudian dilakukan analisis Signal to Noise Ratio (SNR) dan Contrast to Noise Ratio (CNR) serta analisis terhadap Susceptibility  Artifacts dan Chemical Shift. Variasi Time Echo dan bandwidth berpengaruh signifikan terhadap nilai SNR dan CNR pada hasil citra T2 Axial Gradient Echo MRI Shoulder. Pengaruh kedua variasi tersebut adalah semakin kecil nilai TE dan BW didapatkan SNR yang meningkat. Jika TE diperbesar maka didapatkan CNR yang meningkat. Kualitas citra optimal yang dapat menghasilkan keadaan patologis ditentukan dengan meningkatnya nilai CNR dan nilai artefak yang menurun. Sehingga pada penelitian ini kombinasi antara 2 variabel tersebut dapat menentukan citra optimal pada TE 20ms dan BW 210 Hz/Px.
Potensi Blue Light Emitting Diode (LED) untuk Fotoinaktivasi Bakteri Staphylococcus aureus dengan Porifirin Endogen Astuti, Suryani Dyah
Jurnal Biosains Pascasarjana Vol 13, No 3 (2011)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3988.357 KB) | DOI: 10.20473/bsn.v13i3.Y2011.10679

Abstract

Photodynamic Inactivation (PDI) represents a method for bacteria inactivation by using combination of endogenous porphyrin and light photon. The purpose of this study were to determine the instrument performance of LED light source that had been assembled and to recognize the potency of LED irradiating energy dose for photoinactivation of Staphylococcus aureus bacteria ATCC 6538P with endogenous porphyrin. Performance measurement of LED instrument showed that blue LED had emission peak of 430 nm and red 629 nm with 65 nm and 30 nm bandwidth. The analysis of LED irradiating energy dose by anova indicated that interaction of power and irradiating time of blue LED 430 nm decreased the percentage of Staphylococcus aureus colonies. With optimal irradiating of 75 mW/cm’ power density and 30 minute time of irradiating (energy density 135 J/cm’), it yielded the percentage decrease of Staphylococcus aureus colonies as many as 75%. Hence, the blue LED instrument has a high potential for photoinactivation to Staphylococcus aureushacteria.Key words: photodynamic inactivation, LED, endogenous porphyrin photosensitizer, Staphylococcus aureus