Prihartini Widiyanti, Prihartini
Faculty of Science and Technology, Universitas Airlangga, Surabaya, Institute of Tropical Disease (ITD), Universitas Airlangga, Surabaya

Published : 32 Documents
Articles

Found 29 Documents
Search
Journal : Indonesian Journal of Tropical and Infectious Disease

The Utilization of Achatina fulica Mucus in Alginate Membrane as Wound Healing Accelerator and Anti- Infection Material Rahmawati, Fatkhunisa; Mayasari, Dita Ayu; Adhitioso, Satrio; Putra, Alfian Pramudita; Kuntjoro, Eko Budi; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 1 (2014)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Wound should be covered with bandage that is called wound dressing. Most people use synthetic materials such as gauze dressing. Gauze has high absorption of NaCl, which is often used to cleanse the wound. However, discomfort and pain arise since the gauze becomes sticky on the wound. Therefore, we need other alternatives instead of gauze to cover wound. One such alternative is the alginate membrane. This study used alginate membrane with mixture of mucous of the snail Achatina fulica, which contain proteins such as proline, serine asparagine, glycosaminoglycan, hydroxylysine, trionin and so forth, to activate the growth factor. Alginatepowder and carboxymethl cellulose (CMC) was dissolved in distilled water mixed with mucus of the snail Achatina fulica in four variations (4:0; 4:1, 4:2, 4:3) through a magnetic stirrer, and casted on a baking sheet covered with sterile gauze. High Performance Liquid Chromatography (HPLC) test showed that the glycosaminoglycan content was found on the mucous of Achatina fulica. This was indicated by the appearance of peak at 325–350 second. The most optimum alginate and mucus composition was in ratio of 4:2. This ratio resulted in a wound dressing that was still able to absorb the exudate and optimally accelerated wound healing.
TUBERCULOSIS COUNTER (TC) AS THE EQUIPMENT TO MEASURE THE LEVEL OF TB IN SPUTUM Purwanda, Fendy; Fibriawan, Yufan; Sasmito, Dyar; Rahmawati, Fatkhunisa; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.604 KB)

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. This disease is the third killer disease aftercardiovascular diseases and respiratory diseases, and is also he number one killer disease in a group of infectious diseases. This is partly due to the late handling and a non real time detection, both of which will inhibit the therapy which yields a large numberof microorganisms in the body, and will eventually complicate the recovery. Based on this phenomenon, we offered an alternativesolution for detecting the sum of microorganism using Tuberculosis Counter, a tool used to count the number of Tuberculosis bacteria in the patients sputum. Technically, the patients sputum preparat was screened using the TCS230 color sensor that was able to filter the color of the preparat. Tuberculosis bacteria in the stained sputum Ziehl-Niellsen preparat was colored red, while the other was colored blue. By utilizing these optical phenomena, the TCS230 color sensor was supposed to filter the red color in the preparat. By using regression equation measurement, we gained the equation which then correlated the bit value as an output of the sensor with the number of Tuberculosis bacteria. Then, the digitalization process yielded the real time and accurate data of Tuberculosis bacteria.
ANALYSI S ON SECONDARY I NFECTI ON- TRI GGERI NG MICROORGANISMS IN HIV/AIDS PATIENTS AS A MODEL FOR POLICY CONTROL Rahayu, Retno Pudji; Nasronudin, Nasronudin; Indrawati, Retno; Widiyanti, Prihartini; Lukito, Bimo Dwi; Ferdiansyah, Ferdiansyah; Khairunisa, Siti Qomariyah; Kotaki, Tomohiro
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 4 (2014)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

HIV infection is associated with immune-compromised and rising in opportunistic infection (secondary infection). Therefore, the number of mortality caused by HIV/AIDS is increasing. The use of ARV and development of HIV/AIDS management are expected to suppress the progress of HIV infection into AIDS and, therefore, the mortality can be diminished, while in fact most of the patients eventually suffer from AIDS due to secondary infection that commonly causes death. There should be a management by analysing microorganisms that trigger secondary infection. The method of this study was observational descriptive with cross sectional design. HIV infected blood samples were using ELISA Antibody (IgG and IgM) and Polymerase Chain Reaction (PCR) on laboratory test. The result showed correlation between HIV/AIDS severity and the amount and types of secondary infection. The most common secondaryinfections were toxoplasm (96.77%), hepatitis C (22.58%), tuberculosis (19.35%), and hepatitis B (3.22%). Other less frequent secondary infections, which were quite difficult to diagnose and not commonly found in Indonesia, were West Nile Virus (25.81%), Japanese Encephalitis Virus (3.22%), and Enterovirus (3.22%). Due to MDGs (Millenium Development Goals) target and the results above, researchers are highly demanded to contribute in decreasing mortality related to AIDS through early detection of secondary infection,including type of infection which have not been commonly found in Indonesia, such as West Nile Virus and Nipah Virus. The discovery of secondary infection in this study was not enough to suppress the occurrence of infection in HIV/AIDS patients. Antimicrobes and good nutrition are required. Moreover, there should be either a primary or secondary prophylaxis to prevent secondary infection that raises the number of mortality and morbidity of HIV/AIDS patients. The result of this study was to meet the target of MDGs by establishing new policies in handling HIV/AIDS infections and have potential as model for policy control in HIV/AIDS.
COLOSTRUM-COLLAGEN-HYDROXYAPATITE COMPOSITE, AN EXCELLENT CANDIDATE BIOMATERIAL FOR BONE REPAIR AND BONE INFECTION MANAGEMENT Setiawan, Dio Nurdin; Anwar, Mirzaq Hussein; Putri, Kholifatul Wanda; Fiddarain, Nilna Faizah; Widiyanti, Prihartini; Purnobasuki, Heri
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 2 (2014)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In the case of bone fracture or defect after surgery, which is common in patients with bone cancer (osteosarcoma), it takes a long time for closure and it may cause an infection problem. The use of collagen-hydroxyapatite composite with a blend of colostrum as a scaffold is aimed to accelerate the process of osteoblast growth, inhibite the emergence of infections, and act as bone tissue repair material. The method used was the hydrogel formation process and freeze dry process to remove the solvent and to form pores. The composition of scaffold composite manufactured was 15% collagen, 75% hydroxyapatite and 10% colostrum. Combination of scaffold collagen-hydroxyapatite-colostrum has quite reliable properties because SEM test showed that scaffold could bind to both and could bind to both and could form sufficient pores to provide enough place for bone cells (osteoblats) to grow. The results of MTT assay revealed percentage of above 60%, which indicates that the material is not toxic. In conclusion, collagen-hydroxyapatite-colostrum combination is an excellent biomaterial candidate for bone repair and bone infection management.
USING LEARNING VECTOR QUANTIZATION METHOD FOR AUTOMATED IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS Purwanti, Endah; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 1 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.655 KB)

Abstract

In this paper, we are developing an automated method for the detection of tubercle bacilli in clinical specimens, principally the sputum. This investigation is the first attempt to automatically identify TB bacilli in sputum using image processing and learning vector quantization (LVQ) techniques. The evaluation of the learning vector quantization (LVQ) was carried out on Tuberculosis dataset show that average of accuracy is 91,33%.
The Prevalence of Human Immunodefiency Virus-1 (HIV-1) Subtypes and Transmission Method among HIV/AIDS Infection Patient in Tulungagung, East Java Indonesia Ardianto, Achmad; Khairunisa, Siti Qamariyah; Kotaki, Tomohiro; Witaningrum, Adiana Mutamsari; Qushay, M.; Juniastuti, Juniastuti; Rahayu, Retno Pudji; Widiyanti, Prihartini; Utomo, Budi; Lusida, Maria Inge; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The rapid epidemic growth of HIV is continuing in Indonesia. There are some factors which have influenced the spreading of this epidemic in Indonesia, such as the poor awareness to avoid unsafe free sex attitude and the sharing of needles and syringes among intravenous drug users (IDUs). The sexual transmission of HIV has also apparently increased in Tulungagung. Commercial sex workers play a significant role in the spread of HIV in Tulungagung. People in Tulungagung have worked at other countries as Indonesian migrants. This condition can cause the increase number of HIV-1 case and the possibility of genetic variation (subtype) HIV-1 in Tulungagung. This research is aimed to analyze the subtype and to determine estimation of transmission mode on infected patient of HIV-1 and AIDS who came to Seruni clinic Dr. Iskak hospital in Tulungagung. 40 HIV?AIDSpatients were interviewed to determine the subtype and the transmission mode. The results showed that 14 of 40 plasma samples (35%) were successfully to amplified and sequenced. OverallCRF01-AE wereidentified as predominant subtype among HIV/AIDS patients in Tulungagung. Based on individual information, 31 of 40 subjects (77%) were heterosexual transmission.
THE ROLE OF HYPERBARIC THERAPY IN THE GROWTH OF CANDIDA ALBICANS Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.873 KB)

Abstract

Background: Candida albicans is opportunistic pathogen fungi which cause many disease in human such as reccurrent apthous stomatitis, skin lesions, vulvavaginitis, candiduria and gastrointestinal candidiasis. Aim: Infection mechanism of C. albicans is very complex including adhesion and invasion, morphology alteration from khamir form cell to filamen form (hifa), biofilm forming and the avoidance of host immunity. Method: The ability of C. albicans to adhere to the host cell which is act as important factor in the early colonization and infection. Result: The phenotype alteration to be filament form let the C. albicans to penetrate to the epithelium and play important role in infection and separation C. Albicans to the host cell. Hyperbaric oxygen is the inhalation of 100 percent oxygen inside hyperbaric chamber that is pressurized to greater than 1 atmosphere (atm). Conclusion: The organism was found to be inhibited within a pressure/time range well tolerated by human subjects, suggesting that hyperbaric oxygen might be used successfully in treating human candidiasis.
DIGITAL DETECTION SYSTEM DESIGN OF MYCOBACTERIUM TUBERCULOSIS THROUGH EXTRACTION OF SPUTUM IMAGE USING NEURAL NETWORK METHOD Arisgraha, Franky; Widiyanti, Prihartini; Apsari, Retna
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 1 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.924 KB)

Abstract

Tuberculosis (TBC) is an dangerous disease and many people has been infected. One of many important steps to control TBC effectively and efficiently is by increasing case finding using right method and accurate diagnostic. One of them is to detect Mycobacterium Tuberculosis inside sputum. Conventional detection of Mycobacterium Tuberculosis inside sputum can need a lot of time, so digitallydetection method of Mycobacterium Tuberculosis was designed as an effort to get better result of detection. This method was designed by using combination between digital image processing method and Neural Network method. From testing report that was done, Mycobacterium can be detected with successful value reach 77.5% and training error less than 5%.
The Effect of Gendarussin a Isolates of Justicia gendarussa Burm.f. Leaf in Reverse Transcriptase Inhibition of HIV Type I In Vitro E. W., Bambang Prajogo; Widiyanti, Prihartini; Nasronudin, Nasronudin; Aksono, Bimo
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.
BASIC MECHANISM OF HYPERBARIC OXYGEN IN INFECTIOUS DISEASE Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.726 KB)

Abstract

Hyperbaric oxygen therapy (HBOT) is the inhalation of 100 percent oxygen inside a hyperbaric chamber that is pressurized to greater than 1 atmosphere (atm). HBOT causes both mechanical and physiologic effects by inducing a state of increased pressure and hyperoxia. HBOT is typically administered at 1 to 3 atm. While the duration of an HBOT session is typically 90 to 120 minutes, the duration, frequency, and cumulative number of sessions have not been standardized. HBO has been use widely in treating gangrene diabetic, stroke, osteomyelitis and accelerating wound healing. The use of HBO in infectious disease is wide, so the mechanism of hyperbaric oxygen in infectious disease should be well-understand. This understanding could bring the proper and wise management of infectious disease and to prevent the side effect of each therapy.