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VIRULENCE STATUS OF Phytophthora infestans (Mont.) de Bary IN WEST JAVA TO POTATO PLANTS THAT CONTAIN RB GENE Susanti, Hani; Esyanti, Rizkita Rachmi; Sofiari, Eri
Widyariset Vol 16, No 2 (2013): Widyariset
Publisher : LIPI-Press

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Abstract

Phytophthora infestans (Mont.) de Bary causes late blight (LB) disease which can decrease potato (Solanum tuberosum L.) production up to 100%. RB gene as source of resistance to LB could be obtained from genetically modified potato cultivars with resistance genes from wild species Solanum bulbocastanum. Evaluation virulences of P. infestans to potato contain RB gene is an important step to know effectiveness of controlling this disease. The research was focussed on determining virulence status of the isolates to the transgenic and hybrid potato plants containing RB genes. Isolates were collected from Bandung, Cianjur, and Garut, were cultured on V8-agar medium at 18 ± 20C. Virulence test were performed using “detached leaves assay” method. Based on statistic analysis, the average leaf infected by LB in potatoes containing the RB gene was significantly lower than potatoes in control group. Results showed that 40,8% transgenic leaves and 54,5% hybrid leaves which containing RB gene attacked by LB , whereas the control group was infected 98,7%. The virulence level of P. infestans isolates from West Java on potato plants contain RB gene is still low compare to that in the control group.
CULTIVATION OF FILAMENTOUS CYANOBACTERIA FOR VALUABLE BIOPRODUCT USING SAGO SOLID WASTE AS SUBSTRATES Susanti, Hani; Lestari, Sari; Rahman, Delicia Y.; Susilaningsih, Dwi
Teknologi Indonesia Vol 37, No 1 (2014)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.051 KB) | DOI: 10.14203/jti.v37i1.215

Abstract

Sago solid waste as by product result of sago palm processing potentially have great value for some bio processing such as source of carbon on microbes substrate. Utilization of sago solid waste as substrate for filamentous cyanobacteria cultivation is a rareresearch activity in Indonesia as one of the three leading world companies producer of sago starch. Therefore, we focused on sago waste treatment using cyanobacteria agent in order to rid the waste and gain the value of nutrition rich starch from that waste. Several concentrations of growth media contain sago solid waste were tested into cyanobacteria culture and solid waste hydrolysis as pretreatment more conducted in the research. Result shows that addition of sago solid waste (50, 500 and 5000 ppm) into media can increase the algae biomass production which is rich of carbohydrate and protein.The proximate value of biomasses with sago solid waste treatment was exhibited significantly higher than control. During cultivation exposure time, the solid waste is gradually consumed by the algae. The study implies that sago solid waste can be used as substrate for the biomass production of filamentous cyanobacteria which is known as source of active compounds, potential resource for valuable bioproduct.
ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Susilaningsih, Dwi; Umoro, Asahedi; Ochieng, Fredrick Onyango; Widyaningrum, Dian Noverita; Susanti, Hani; Susilo, Hadi; Swastika, I Nengah; Widyastuti, Utut
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4410.754 KB) | DOI: 10.14203/beritabiologi.v18i2.2967

Abstract

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ?Carita? Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids. 
EFIKASI LIMBAH SAGU SEBAGAI SUBSTRAT KAYA NUTRISI UNTUK MIKROALGA ISOLAT LIPI11-2-AL002 Susilaningsih, Dwi; Lestari, Sari; Kusnadi, Kusnadi; Hidayat, Topik; Susanti, Hani
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.674

Abstract

Microalgae are photosynthetic microorganisms that have potential to produce some useful chemical substances such as carbohydrates, proteins, and lipids. Microalgae are also known exhibited ability as a bioremediation agent. This report is emphasized on analyzing the growth rate and nutritional content of microalgae including carbohydrate, protein and lipid from selected isolates LIPI11-2-AL002 that treated with sago waste. Microalgae were cultivated into hydrolysed sago ?ampas? at the concentration of 0,50, 500,5000 ppm, and medium AF-6 (as control culture) respectively.Therefore selected microalgae isolate was cultivated in series of cultivation volume start from 100 mL until 5 Liter media gradually. Observed parameters were covered cell viability (growth) and proximate content of biomass including carbohydrate,lipid and protein content. The results showed that the carbohydrate and protein content in the algal biomass was increase along the addition of series sago ?-ampas? concentration.The highest concentration of addition the sago ampas is 5000 ppm which is limit for the algal survival. In the highest treatment of sago waste the alga l proximate contents were 261.09 ppm of carbohydrate, 5.12 ppm of protein and 3.61% per of lipid dry weight respectively. In addition, the toxicity effect of fermentation product was not appeared in a toxicity test using gold fish komet(Carassius auratus).
PENANGANAN ANAK KESULITAN BELAJAR DISLEKSIA MELALUI PERMAINAN BOWLING KEBERANIAN Safarina, Eka Sri; Susanti, Hani
CERIA (Cerdas Energik Responsif Inovatif Adaptif) Vol 1, No 2 (2018): Volume 1 Nomor 2, Maret 2018
Publisher : IKIP Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.509 KB) | DOI: 10.22460/ceria.v1i2.p35-40

Abstract

Handling Children Difficulties Learning Dyslexia through Bowling Game Courage aims to find out what is the difficulty of learning dyslexia, its causal factors, its type and characteristics, even to the way to overcome them. The method used in this research using qualitative methods. Children with special needs that vary such as Autism, mental illness, paralyzed, until the children who have trouble learning difficulties are also included in learners who have privileges. A special child is a child who has a weakness in physical function and cognitive to develop well. The cause varies from the disturbance during pregnancy, interruption at the time of childbirth, to the violence in children, it can cause disruption to the learning of children. This weakness is a weakness that can?t be seen with the eyes like wrong at the time of counting, reading and writing. Therefore there is one way to make learners who get problems with learning, especially reading can be helped with the game of letters and numbers, so that children do not feel bored and quickly memorized with interesting games such as bowling games with courage in addition to children can know the letters and numbers, children can also move by following the motion attached to the bowling pin, the child can also count the number of bowling pins that fall, mention the color of the falling pin, then the results of research that has been done from 3 learners who have problems in reading show good results with already able to mention 3 letters. Keywords: Learning Difficulties, Dyslexia, Courage game of courage.