R. R. Noor, R. R.
Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan IPB Bogor 16680

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Polymorphism of stearoyl-CoA desaturase (SCD1) gene in Indonesian local cattle Hilmia, N.; Noor, R. R.; Sumantri, C.; Gurnadi, R. E.; Priyanto, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 1 (2017): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.456 KB) | DOI: 10.14710/jitaa.42.1.1-5

Abstract

Stearoyl-Coa desaturase (SCD1) gene is one of genes that involves in converting saturated fatty acids to unsaturated fatty acids. SNP at exon 5 in SCD1 gene that changes amino acid valine to alanine (V293A) has an influence to meat fatty acid composition. The aim of this research was to analyze SCD1 gene polymorphisms based on SNP V293A at exon 5 of three Indonesian local cattle. The identification of SCD1 gene polymorphisms was done by using 98, 20 and 7 DNA sample from Ciamis, Bali/Banteng, and Ongole Grade (PO) cattle, respectively. PCR_RFLP method with AciI enzim was carried out to identify SNP Val293Ala. Allelic frequencies and heterozygosity value were analyzed by using POPGENE32. The result showed that SCD1 gene at Ciamis local cattle and PO cattle were polymorphic. Their frequencies were 74.5% and 71.4% for T and 25.5% and 28.6% for C, respectively. There were three genotypes on Ciamis local cattle i.e TT, CT and CC with their frequencies were 52%, 44.9% and 3.10%, respectively. There were two genotypes on PO cattle i.e TT and CT with their frequencies were 42.9% and 57.1%, respectively. Meanwhile, SCD1 gene in Bali cattle was monomorphic. Heterozygosity value of SCD1 gene in Ciamis and PO cattle were 0.38 and 0.44, respectively. Their heterozigosities were categorized as medium. 
Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep Munyaneza, J. P.; Gunawan, A.; Noor, R. R.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.473 KB) | DOI: 10.14710/jitaa.44.3.243-251

Abstract

This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in lambs. This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT), 16 Javanese Thin-Tailed (JTT), 41 Composite Garut (CG), 35 Compass Agrinak (CA) and 36 Barbados Black Belly Cross (BC). A total of 61 rams as representative from five breed of sheep were selected for association study. Identification of BHMT single nucleotide polymorphisms was analyzed by PCR-RFLP method. Association of BHMT genotypes with fatty acid traits and cholesterol was performed by T-TEST. BHMT genotyping resulted into three genotypes (CC, CT and TT). Gene frequency of BHMT (g. 9947372 C>T) was in Hardy-Weinberg Equilibrium, excluding Javanese Fat-Tailed sheep. Association of BHMT genotypes with fatty acid traits resulted into a significant association (P<0.05) with C14:1, C17:1 and C18:0 fatty acids but not with cholesterol in sheep. SNP g. 9947372 (C>T) of BHMT gene might be a useful marker for selecting and producing sheep meat with desirable fatty acids. 
SINGLE NUCLEOTIDE POLYMORPHISMS OF LIPOPROTEIN LIPASE GENE AND ITS ASSOCIATION WITH MARBLING QUALITY IN LOCAL SHEEPS Hidayati, H.; Sumantri, C.; Noor, R. R.; Priyanto, R.; Rahayu, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 1 (2015): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.929 KB) | DOI: 10.14710/jitaa.40.1.1-10

Abstract

Lipoprotein lipase (LPL) is a key enzyme that plays in metabolism and transport lipoprotein andtherefore has an influence on blood triglyceride levels. LPL controls triacylglycerol partitioning betweenadipose tissue and muscle that increases fat storage or provides energy in the form of fatty acids formuscle growth. The research was aimed to explore Single Nucleotide Polymorphisms of LPL gene andto associate SNP with marbling quality. A total of 66 genomic DNAs consisted of sumatera thin-tail edsheep (50 heads) and garut sheep (16 heads) were used in this study. Polymerase Chain Reaction wasused to amplify genomic DNA and direct sequencing method was to identify polymorphism sequences.The sequences were analyzed with Bio Edit and MEGA 5.2. The BLAST sequence was obtained fromgene bank X.68308.1. The association between the genotype and marbling quality was analyze by oneway ANOVA and further between mean differences were tested using least sgnificant difference. Theresults showed that 3 novel SNPs i.e. insertion g.26>C; insertion g.27> G and c.192T>C on garut sheepand a SNP insertion g.26>C/G on sumatera thin-tail ed sheep. The diversity of LPL gene at c.192T>Cwas associated with heneicosanoic acid, whereas TT genotype (0.04%) was higher than CC (0.03%) andCT (0.02%).
Efektivitas Catatan Test Day untuk Evaluasi Genetik Produksi Susu pada Sapi Perah Indrijani, H.; Anang, A.; Noor, R. R.; Talib, C.
Zuriat Vol 14, No 1 (2003)
Publisher : Zuriat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Penelitian ini bertujuan untuk mempelajari efektivitas catatan Test day dengan model regresi tetap untuk evaluasi genetic produksi susu pada sapi perah berdasarkan laktasi satu dan dua. Data produksi susu berasal dari sapi perah Frisian Holstein periode laktasi satu dan dua dari tahun 1989–2000 di PT. Taurus Dairy Farm Sukabumi. Catatan Test day sebanyak 7503 buah berasal dari 456 ekor sapi betina, 40 pejantan dan 342 ekor induk. Model yang digunakan adalah Model Regresi Tetap, varian komponen diduga dengan Animal Model dengan Restricted Maximum Likelihood (REML) dan Nilai Pemuliaan dengan Best Linier Unbiased Prediction (BLUP). Hasil analisis menunjukkan hari test ke-35 (TD2) merupakan puncak produksi. Pengaruh musim terhadap produksi susu Test day mempunyai pola yang kurang jelas. Ragam genetik aditif pada laktasi dua menurun sejalan dengan penambahan catatan Test day sampai dengan Test day ke-3, setelah itu meningkat sampai akhir laktasi. Dugaan nilai heritabilitas mengikuti pola yang sama dengan ragam genetik. Evaluasi genetic berdasarkan laktasi satu penuh yang ditambah dengan satu catatan Test day pada laktasi dua sudah cukup untuk mengevaluasi seluruh ternak, sedangkan untuk mengevaluasi pejantan, evaluasi harus diperpanjang sampai 3 catatan Test day pada laktasi dua.
Profil Hormon Progesteron dan Gen Fekunditas terhadap Sifat Kembar Kambing PE Betina Calon Induk Mulyono, R. H.; Sumantri, C.; Noor, R. R.; Jakaria, Jakaria; Astuti, D. A.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol 6, No 1 (2018): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.6.1.19-26

Abstract

Etawah-grade goat is a graded up line between kacang and etawah goats, which well adapted to Indonesia’s humid tropical climate. The good reproductive trait of Etawah-grade does are inherited from kacang goat (prolific goat). This study used six heads of Etawah-grade does at 1–2 years old (I1 dental condition). IPB University. The body variables were withers height, hip height, body length, chest width, chest depth, thurl width, rump length, chest girth, and cannon circumference, whereas for head variables were acrocranion–prosthion, basion–prosthion, lower jaw length, head height, tuber facial left-right, nasion–rhinion, entorbitale left-right, euryon left-right, supraorbitale left-right. Body score and head score were obtained from Principal Component Analysis (PCA). PCR-RFLP technique was used to determine genotypes of fecundity genes BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1) and KISS1 (intron 1) with restriction enzymes AluI (AG|CT), SduI (GDGCH|C), BsrI (ACTGG|) and MwoI (GCCTAAG|TAGC), respectively. Progesterone leves were measured by EIA (enzyme immuno assay). The results showed that the association between crude fat consumption and progesterone profile was found in this study, but it was not related to twin births with certain genotypes of gene fecundity BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1) and KISS1 (intron 1). The progesterone profile during the pregnancy period did not correlate to their body size and head size.
Identifikasi Ukuran Tubuh dan Bentuk Tubuh Sapi Bali di Beberapa Pusat Pembibitan Melalui Pendekatan Analisis Komponen Utama Hikmawaty, Hikmawaty; Gunawan, A.; Noor, R. R.; Jakaria, Jakaria
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol 2, No 1 (2014): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.981 KB) | DOI: 10.29244/jipthp.2.1.231-237

Abstract

Bali cattle is indigenous livestock genetic resources which have distinctive features and ability to grow well in a variety of environments in Indonesia. The aim of this study was to determine the phenotypic profile of Bali cattle in some breeding centers which represented by BPTU Pulukan Bali, VBC Kab. Barru South Sulawesi and BPTHMT Serading NTB. Data were analyzed by using Principal Component Analysis (SAS) to determine the size identifier which was influenced by the environmental factors and genetic factors form  the basic information on the characteristics of Bali cattle. The parameters such as dimensions and body weight of Bali cattle due to the possibility of differences in genetic potential, location of origin, implementation of the mating system and environmental management including maintenance were observed. The result showed Bali cattle body size on BPTHMT Serading NTB  were smaller (P <0.5) compared to the body size of Bali cattle in BPTU Pulukan Bali and VBC Kab. Barru South Sulawesi while Bali cattle in BPTU Pulukan Bali and VBC Kab. Barru South Sulawesi were relatively similar. Chest girth measured on the body has the highest correlation with body weight in Bali cattle both in males and females in different breeding centers.The size score and the shape between Bali cattle breeding centers in the male and female Bali cattle showed its own characteristics due to their respective locations. Identifier the size of Bali cattle at NTB Serading BPTMHT showed relatively smaller than Bali Cattle in BPTU Pulukan Bali and VBC Kab. Barru SouthSulawesi. Identifier the same shape among all three populations showed the breeding centers have the samecharacteristic genetically.