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PEMANFAATAN AIR KELAPA (Cocos nucifera) UNTUK MASKULINISASI IKAN GUPPY (Poecilia reticulata) Dwinanti, Sefti Heza; Putra, M. Hanggara; Sasanti, Ade Dwi
Jurnal Akuakultur Rawa Indonesia Vol 6, No 2 (2018): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

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Abstract

ABSTRACT               Masculinization is a method of  sex reversal technique that can direct sexuality from female to male. The purpose of this study was to determine the effects of coconut water on masculinization in guppy by immersion administration. The study used a completely randomized design (CRD) with three treatments and three replications. After 12 days post spawning, female guppies were immersed for 8 hours.The concentrations were : 0%(P0), 20% (P1), and 40% (P2). The parameters were observed including percentage of male, survival rate and water quality (thermal, pH and kalium concentration). Baby guppies were reared until sex can be determined which were 42 days of culture. The result showed that the percentage of male guppy fish was 54.44% (P0), 77.78% (P1) and 90.00% (P2). The survival rate of  larval during maintenance was 100% for all treatments. Water quality during maintenance was 27.0-28.40C for temperature and 6.38-7.20 for pH. To sum up, this experiment proved that coconut water can be used for masculinization by immersed pregnant female broadstock and it produced 90% male when immersed with 40% of coconut water. Keywords : Coconut water, guppy (Poecilia reticulata), masculinization
TOKSISITAS DAN IMUNOGENISITAS PRODUK EKSTRASELULER Streptococcus agalactiae TIPE NON-HEMOLITIK PADA IKAN NILA (Oreochromis niloticus ) Dwinanti, Sefti Heza; ., Sukenda; Yuhana, Munti; M Lusiastuti, Angela
Jurnal Akuakultur Rawa Indonesia Vol 2, No 1 (2014): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

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Abstract

ABSTRACT The aim of this research was to analyse the toxicity and immunogenicity of ECP of non-hemolytic S. agalactiae and to evaluate ECP as a material vaccine for prevention of S. agalactiae. Toxicity to tilapia was detected in the ECP. There was a chronic  mortality pattern which was shown after post ECP injection. By intraperitoneal injection into young tilapia (± 20 g), the median lethal dose were calculated at 633,9 µg/kg bodyweight for isolate 3 and 685,4 µg/kg bodyweight for isolate 5. Cytopathic effects of ECP to tilapia’s lymphocytes were observed during 0, 30, 60 and 180 minutes. A mixture of ECP caused nuclear granulation and cytoplasmic streaming after 60 minutes. Using immunodiffusion analysis, precipitate line was demonstrated against tilapia anti-ECP sera, and there was bio-specific type anti sera against for each isolate. The ECP vaccine effective to protect tilapia from S. agalactiae was injected intraperitoneally (IP) at 2 x 105 CFU/ml. Tilapia vaccinated with ECP from isolat 3 which protein  283 µg/kg bodyweight had a RPS value 60%.  Tilapia vaccinated with ECP from isolat 5 which protein 408 µg/kg  had a RPS value 68%.   Keywords:  toxicity, immunogenicity, extracellular products, Streptococcus agalactiae, tilapia
MODIFIKASI MEDIA SPIRULINA PLATENSIS SEBAGAI UPAYA PEMANFAATAN AIR LIMBAH BUDIDAYA IKAN LELE Widyantoro, Hendro; Wijayanti, Marini; Dwinanti, Sefti Heza
Jurnal Akuakultur Rawa Indonesia Vol 6, No 2 (2018): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

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Abstract

ABSTRACTThe purpose of this study was to determine the best fertilizer compotition that was made from technical fertilizer and waste water of cat fish farming. This experimental was conducted at Aquaculture Laboratory, Faculty of Agriculture, Sriwijaya University. It used complete randomized design (CRD) method which had five treatments and three replications, the waste water from catfish pond (P0), waste water from catfish pond + 25% technical fertilizer (P1), waste water from catfish pond + 50% technical fertilizers (P2), the waste water from catfish pond + 75% technical fertilizers (P3), the waste water from catfish pond + 100% technical fertilizers (P4). The results showed that the best maximum density of S. platensis was P3 about 3.98 g l-1, and growth rate was equal to 2.80% day-1. The most significant parameter of water quality was ammonia which decreased until 82.78%. Besides, it was more beneficial than others based on Return Cost Ratio (R/C ratio) and Benefit Cost Ratio (B/C ratio) which were 3.66 and 2.66 respectively. Keywords: Spirulina platensis, waste water pond of catfish, modified  media