I Wayan Masa Tenaya, I Wayan Masa
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Profil Hormon Pertumbuhan Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali (THE GROWTH HORMONE PROFILE OF BALI CATTLE’S IN THREE TYPES OF PRESERVATION AREA IN BALI PROVINCE) Milfa, Sri; Suwiti, Ni Ketut; Tenaya, I Wayan Masa
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine of Udayana University

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Abstract

The objective of this study was to determine the growth hormone profile of bali cattle’s whichwas reared in three different types of preservation areas in Bali Province namely gardens, fields andcultivated lands. The sample of this study was serum taken from 78 bali cattles which spread inthose three areas. The growth hormone concentration was tested from the serum using ELISA(Enzyme Linked Immunosorbent Assay). The results showed that the highest concentration of growth hormone of bali cattle’s in gardens was 3225,19 pg/ml and the lowest was 931,17 pg/mlwith mean was 1311,38±128,4. In fields area, the highest concentration of growth hormone was1852,91 pg/ml and the lowest was 906,22 pg/ml with mean was 1185,89±41,52, and in thecultivated land, the highest concentration of growth hormone was 5044,08 pg/ml and the lowestwas 1046,83 pg/ml with mean was 1927,64±246,64. This study concluded that the profile ofgrowth hormone concentration of bali cattle’s reared in gardens, fields, and cultivated land weredifferent. The highest growth hormone concentration was found in cultivated land(1927,64±246.64) and the lowest was in fields (1185,89±41.52).
Respons Imun Mencit yang Diimunisasi dengan Cysticercus cellulosae (IMMUNE RESPONSE TO TAENIA SOLIUM CYSTICERCOSIS IN MICE) Swacita, Ida Bagus Ngurah; Damriyasa, I Made; Dharmawan, Nyoman Sadra; Astawa, Nyoman Mantik; Apsari, Ida Ayu Pasti; Tenaya, I Wayan Masa
Jurnal Veteriner Vol 16, No 2 (2015)
Publisher : Jurnal Veteriner

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Abstract

Cysticercosis is a zoonotic parasitic disease which is still problem in Indonesia. The purpose of thisstudy was to investigate immune responses of mice that had been immunized using Taenia solium larval(Cysticercus cellulosae) antigens originated from infected pigs. Three kinds of the C.cellulosaeantigens,secretory and whole antigens were used to immunize three different groups Balb/c mice consisted of 15animals. The serum samples before and after immunization were tested with ELISA test. The resultsshowed that the third antigens induced highly significant titre (P<0,01)compared to unimmunized animals.However no significant different (P>0,05) were found when the third antigens were compared. It wasconcluded that immunization with the three kinds of C. cellulosae antigens can cause immunity in mice.
Produksi dan Karakterisasi Antibodi Monoklonal Anti-Cysticercus cellulosae (PRODUCTION AND CHRACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CYSTICERCUS CELLULOSAE) Swacita, Ida Bagus Ngurah; Damriyasa, I Made; Dharmawan, Nyoman Sadra; Astawa, Nyoman Mantik; Apsari, Ida Ayu Pasti; Oka, Ida Bagus Made; Tenaya, I Wayan Masa
Jurnal Veteriner Vol 16, No 3 (2015)
Publisher : Jurnal Veteriner

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Abstract

The purpose of this study is to make a monoclonal antibody against- Cysticercus cellulosae and itscharacterization. Samples antigen prepared from T. solium larvae (C. cellulosae) was then used to immunizeBalb/c. The immune response of mice assessed by ELISA test, then the lymphocytes of mice used for theproduction of monoclonal antibodies (MoAb). Origin lymphocytes of mice that produce antibodies againstC. cellulosae antigen, fused with myeloma cells (NS1). Results fusion of two cells produces hybrid cellscalled hybridomas; cells are then screened by ELISA test. Hybridoma cells that produce only MoAb, usedto produce large quantities in vitro. Characterization of MoAb against-C.cellulosae was tested by usingELISA and Western blotting. Mice were immunized with C.cellulosae antigen showed an immune responseproducing antibodies to C.cellulosae. Based on the results of fusion, produced a total of 51 hybridoma cellclones and after being screened, only three clones of hybridoma cells that produced MoAb against–C.cellulosae. MoAb produced, named after the hole where the growth of the ELISA micro plate, the BE6,BE7, and EE9. Characteristics of this MoAb capable of tracking cellulosae of fluid larvae and recognizeantigen protein bands with molecular weight 78kDa.