Muhammad Jalaluddin, Muhammad
Laboratorium Anatomi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

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STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak))

Jurnal Kedokteran Hewan Vol 10, No 1 (2016): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak

Studi Histokimia Sebaran Karbohidrat Usus Biawak Air (Varanus salvator)

P-ISSN 2337-3202
Publisher : Bogor Agricultural University

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Abstract

Penelitian ini bertujuan mengetahui sebaran karbohidrat pada usus biawak air dengan metode histokimia. Organ usus dari satu ekor biawak jantan dewasa dikoleksi melalui prosedur perfusi dan selanjutnya difiksasi dengan larutan paraformaldehid 4%. Usus biawak dibagi menjadi enam bagian, yaitu bagian I sampai VI dan selanjutnya diproses menjadi preparat histologi. Deteksi sebaran karbohidrat pada lapisan mukosa usus dilakukan dengan pewarnaan alcian blue (AB) pH 2,5 untuk karbohidrat asam dan periodic acid schiff (PAS) untuk karbohidrat netral. Hasil penelitian menunjukan bahwa sebaran karbohidrat asam ditemukan pada sel goblet pada keenam bagian usus dengan intensitas reaksi sedang (++) sampai kuat (+++), dan tidak ditemukan pada struktur usus lainnya. Sebaran karbohidrat netral ditemukan di seluruh permukaan jaringan usus dengan intensitas reaksi lemah (+) sedangkan intensitas reaksi sedang (++) sampai kuat (+++) ditemukan pada sel goblet. Jumlah sel goblet penghasil karbohidrat asam dan netral pada usus bagian I-IV lebih sedikit (+~++) dibandingkan usus bagian V-VI (+++). Berdasarkan hasil penelitian dapat disimpulkan bahwa sebaran karbohidrat asam dan netral ditemukan diseluruh bagian usus dengan intensitas pewarnaan yang bervariasi. Jumlah sel goblet yang terdeteksi menghasilkan kedua jenis karbohidrat tersebut lebih banyak ditemukan pada usus bagian kaudal.Kata kunci: jaringan usus, sel goblet, karbohidrat asam dan netral, Varanus salvator. (Histochemical Study of Intestinal Carbohydrates Distribution in the Water Monitor (Varanus salvator))The objective of this study was to elaborate the distribution of carbohydrate in intestine tissue of water monitor using histochemical method. Intestinal organ from an adult male water monitor was collected after perfused and subsequently fixed in paraformaldehyde 4%. Intestinal organ was divided to six regions e.g. I to VI and then processed to histological slides. The carbohydrate distribution on the mucosal surface of intestinal tissue was stained with alcian blue (AB) pH 2.5 for detect the acid carbohydrates and periodic acid Schiff (PAS) for the neutral carbohydrates. The results showed that the distribution of acid carbohydrates found in the goblet cells at the I to VI regions with vary intensity of staining reaction with good staining (++) to intense staining (+++), whereas other intestinal structures did not contain the acid carbohydrates. Furthermore, the distribution of neutral carbohydrates was found in the whole intestinal tissue with weak reaction (+), while good staining (++) to intense staining (+++) was appeared in the goblet cells. Additionally, the number of goblet cells containing acid and neutral carbohydrates at the I-IV region was fewer (+~++) than at V-VI region (+++). Conclusion of this study is the distribution of acid and neutral carbohydrates appeared in all regions of the intestinal tissue. The large number of goblet cells that secreting both of carbohydrate type was found at the caudal of intestinal tissue.Keywords: intestinal tissue, goblet cells, acid and neutral carbohydrates, Varanus salvator.

ANALISIS KESESUAIAN DAN KETERSEDIAAN LAHAN SERTA ARAHAN PENGEMBANGAN KOMODITAS PERTANIAN DI KABUPATEN KEPULAUAN MERANTI PROVINSI RIAU

Jurnal Ilmu Tanah dan Lingkungan Vol 14, No 2 (2012): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

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Abstract

Kabupaten Kepulauan Meranti merupakan kabupaten hasil pemekaran dari Kabupaten Bengkalis. Dasar hukum berdirinya Kabupaten Kepulauan Meranti adalah Undang-undang Nomor 12 Tahun 2009, tanggal 16 Januari 2009. Mengingat usia kabupaten ini yang relatif muda, arahan pengembangan komoditas pertanian belum tersedia secara lengkap. Banyak aspek lahan yang harus diperhatikan agar pemanfaatan atau penggunaan lahan untuk pengembangan komoditas wilayah lebih efektif dan berdaya guna. Tujuan penelitian ini adalah: (1) Mengetahui komoditas unggulan di Kabupaten Kepulauan Meranti, (2) Menganalisis kesesuaian lahan untuk pengembangan berbagai komoditas pertanian, (3) Mengkaji dan menganalisis ketersediaan lahan yang berpotensi pengembangan untuk berbagai komoditas pertanian, dan (4) Menyusun arahan pengembangan komoditas pertanian. Hasil penelitian menunjukkan luas lahan sesuai dan tersedia di Kabupaten Kepulauan Meranti untuk pengembangan komoditas unggulan sebesar 108,277 ha. Komoditas yang dapat dikembangkan tiap kecamatan adalah sebagai berikut: Kecamatan Merbau adalah sagu (tanaman perkebunan), ketela pohon (tanaman pangan), jambu biji (buah-buahan) dan cabe besar, cabe rawit dan ketimun (sayur-sayuran); Pulau Merbau adalah karet dan pinang (tanaman perkebunan); Rangsang adalah sawo dan nenas (buah-buahan). Rangsang Barat adalah kopi dan pinang (tanaman perkebunan), pisang, pepaya dan manggis (buah-buahan); Tebing Tinggi adalah ketela rambat dan jagung (tanaman pangan) dan cabe besar, cabe rawit, kacang panjang dan ketimum (sayur-sayuran); Tebing Tinggi Barat adalah sagu (tanaman perkebunan), ketela rambat (tanaman pangan), pepaya, sukun dan nenas (buah-buahan); dan Kecamatan Tebing Tinggi Timur komoditas yang dapat dikembangkan adalah sagu untuk tanaman perkebunan.

Sebaran Karbohidrat pada Kelenjar Ludah Biawak Air (Varanus salvator) (DISTRIBUTION OF CARBOHYDRATES IN THE SALIVARY GLANDS OF WATER MONITOR (VARANUS SALVATOR))

Jurnal Veteriner Vol 15, No 4 (2014)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to determine the carbohydrate content of salivary glands lingual,sublingual, and mandibular glands of water monitor (Varanus salvator). This studywas used the lingualglands, sublingual, and mandibularof an adult male water monitor. Salivary glands were fixed by soakingin a solution of 4% paraformaldehyde for four days. Then, the tissuewas processed into routine histologicalpreparations. Salivary gland tissue was stained with alcianblue staining (AB) pH 2.5 for detecting acidiccarbohydrates and was stained with periodic acid Schiff (PAS) for detecting neutral carbohydrates. Theresults showed that the lingual and sublingual glands showed a positive reaction to AB pH 2.5 stainingand PAS with the intensity varied from weak (+) to strong intensity (+++) scattered in the cytoplasm of thecell. In the cytoplasm of cells of the mandibular glands showed negative reaction (-) to the AB pH 2.5staining but reacted positively to PAS with the intensity varied from weak (+) to strong (+++). Based onthe result of the study it can be concluded that lingual and sublingual glands of Water monitor containacidic and neutral carbohydrates while mandibular glands only contain neutral carbohydrates.

Morfologi Anatomi dan Histologi Usus Biawak Air (Varanus salvator) THE ANATOMICAL AND HISTOLOGICAL MORPHOLOGY OF INTESTINAL WATER MONITOR (VARANUS SALVATOR)

Jurnal Veteriner Vol 16, No 2 (2015)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to determine the anatomical morphology and histology of the intestineof water monitor (Varanussalvator). Intestine organ from an adult male water monito was used in thisstudy. Perfusion was performed to flow the 4% paraformaldehyde fixative solution through the heart.Intestine was collected, then divided into six sections, followed with dipping them into 4% paraformaldehydesolution for four days, continued histological preparations and stained with haematoxylin eosin (HE). Theanatomical morphology observation was performed before the intestine was processed into histologicalpreparations by observing the outer shape of the intestine and measuring it’slength. Morphologicalobservation was made †after processing theintestinal tissue by observing each portion of the intestinalwall structure. The results showed that transverse fold were found on the outer and inner surfaces of theintestine section I-IV, while in the V-VI part of the intestine, the folds were not observed anymore. Theintestine wall consisted of four layers, such as mucosa, submucosa, tunica muscularis, and serosa. Thevilli on intestine section I-IV were relatively longer and higher, while the intestine vili in section V-VI wererelatively short. The entire mucosa in water monitor was coated with absorptive columnar epitheliumwith goblet cells. Based on the result study, it can be concluded that the water monitor has more efficientdigestive process which can be seen morphologically from the relatively short intestinal, has transversefolds on the surface of the small intestine, and does not have cecum. The intestinal wall consists of layersi.e : mucosa, submucosa, tunica muscularis, and serosa.

SURVEI PENGETAHUAN DAN SIKAP PEMILIK RUMAH MAKAN TERHADAP RANTAI PENGOLAHAN DAGING AYAM DI BANDA ACEH (Survey of Knowledge and Attitude of Restaurant Owners on Chicken Processing Chain in Banda Aceh)

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 2 (2017): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Abstract

ABSTRAKPenelitian ini bertujuan mengetahui pengetahuan dan sikap pemilik rumah makan terhadap rantai pengolahan daging ayam di Banda Aceh. Penelitian ini dilaksanakan pada bulan Januari 2017 di beberapa rumah makan di Banda Aceh. Penelitian dilakukan dalam bentuk survei lapangan dengan mewawancarai responden menggunakan kuesioner terstruktur. Responden dipilih secara proporsional terhadap pemilik rumah makan di Banda Aceh. Kriteria responden meliputi berumur ≥ 20 tahun, pemilik rumah makan yang menjual olahan daging ayam, rumah makan dengan kebutuhan daging ayamnya ≥ 20 ekor/hari dan pemilik rumah makan di lokasi penelitian. Data hasil penelitian dianalisis secara deskriptif. Hasil penelitian terhadap 45 responden menunjukkan bahwa pengetahuan pemilik rumah makan terhadap rantai pengolahan daging ayam tergolong baik, namun sikap pemilik rumah makan terhadap rantai pengolahan daging ayam tergolong kurang baik, karena 22,22% responden menggunakan alat potong (pisau) yang sama dengan sayuran, serta 48,89% responden tidak mencuci tangan sebelum mengolah daging ayam.Kata kunci : pengetahuan, sikap, pemilik rumah makan, daging ayam, rantai pengolahan.ABSTRACT This study was aims to determine the knowledge and attitude of restaurant owners on chicken meat processing chain in Banda Aceh. This study was conducted in January 2017 in several restaurants in Banda Aceh. This research was conducted in the form of a field survey by interviewing respondents using a structured questionnaire. Respondents were selected proportionally which is restaurant owners in Banda Aceh. The criteria of respondents were aged ≥ 20 years, restaurant owners who sell chicken, restaurants that needs ≥ 20 chicken / day and restaurant owners in the study site. Data were analyzed descriptively. The results of 45 respondent shows that the knowledge of restaurant owners on chicken meat processing chain is quite good, but the majority of owners attitude towards chicken meat processing chain relatively poor because 22,22% of respondents using the same knife with vegetables and 48,89% of respondents do not wash their hands before treating chicken meat.Keyword : knowledge, attitude, restaurant owners, chicken meat, processing chain.

Jumlah Koloni Bakteri Selulolitik pada Sekum Ayam Kampung (Gallus domesticus)

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Abstract

Penelitian ini bertujuan menghitung jumlah koloni bakteri selulolitik pada sekum ayam kampung. Sampel yang digunakan yaitu isi sekum ayam kampung yang diambil di Tempat Pemotongan Unggas (TPU) Lambaro Aceh Besar sebanyak 5 ekor ayam kampung. Sampel dilakukan pengenceran 10-2 sampai 10-5, kemudian diinokulasi ke dalam media BHM-CMC agar dengan metode tuang (pour plate). Selanjutnya, diinkubasi dengan suhu 37˚C selama 96 jam. Koloni bakteri selulolitik yang tumbuh kemudian dilakukan pengamatan morfologi koloni (warna, posisi, pinggiran, dan diameter koloni), diameter zona bening, penghitungan jumlah total bakteri, dan pewarnaan Gram. Hasil penelitian menunjukkan bahwa koloni bakteri berwarna putih, berada di dalam dan di permukaan agar, memiliki pinggiran rata, diameter koloni 2-3 mm dan diameter zona bening 18 mm dan 29 mm. Bakteri berbentuk basil bereaksi negatif terhadap pewarnaan Gram, sedangkan bakteri berbentuk kokus bereaksi positif dan negatif terhadap pewarnaan Gram. Jumlah total bakteri pada isi sekum ayam kampung yaitu 5.3 x 105 cfu/g.Kata kunci: Ayam kampung, bakteri selulolitik, sekum, Carboxy Methyl Cellulase.                The aim of this study was to count the total count of cellulolytic bacteria colony in caecum of native chicken. The sample used was caecum contents of native chicken which taken from the poultry slaughterplace at Lambaro Aceh Besar. Sample were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Then, incubated with temperature 37oC about 96 hours. A colony of cellulolytic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of the colony), diameter of the bacterial zone, counting a total of bacteria which was grown, and Gram stain. The results showed that there is white bacterial colony, located inside and surface agar, has flat periphery, diameters of colony 2-3 mm and diameters of bacterial zone 18 and 29 mm. Bacill-shaped bacteria react negative to Gram staining, whereas coccus-shaped bacteria react positive and negative to Gram staining. Number of total bacteria in the native chicken’s caecum was 5.3 x 105 cfu/g Keywords: Native chicken, cellulolytic bacteria, caecum, Carboxy Methyl Cellulose.

4. The Value of Erythrocytes, Hemoglobin, and Hematocrit of Mice (Mus musculus) Exposed to Cigarette Smoke and Given Red Watermelon Extract (Citrullus vulgaris)

Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Abstract

The aim of this research was to investigate the effect of red watermelon (Citrullus vulgaris) extract to the value of erythrocytes, hemoglobin, and hematocrit of mice (Mus muscullus) exposed to cigarette smoke. This study followed direct complete randomized design by using 12 males’ mice which divided into 4 groups. The treatment group is divided by the negative control group which given 0,5 ml of aquadest, the positive control group which exposed to cigarette smoke and 0,5 ml of aquadest. The treatment group I was exposed to cigarette smoke and given red watermelon extract dose 22 mg/Bw mice. The treatment group II was exposed to cigarette smoke and given red with watermelon extract dose 44 mg/Bw mice. The exposure to cigarette smoke and the given of red watermelon extract were conducted for 30 days. The blood taking was performed on day 31 in the Plexus retroorbital.  Furthermore, the number of erythrocytes, hemoglobin and hematocrit were calculated. The data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of red watermelon for 30 days showed a highly significant effect (P <0.01) to the number of erythrocytes, hemoglobin, and hematocrit of mice. It is concluded that free radicals contained in the cigarette can cause a hemoglobin desaturation, increased blood viscosity, oxidative stress and red watermelon extract can minimize the damage.

1. Immunohistochemical Study of the Immunoreactive Follicle Stimulating Hormone (ir-FSH) Cells Distribution in Pituitary Gland of Rat(Rattus norvegicus)

Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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The objective of this study was to identified the development of the immunoreactive follicle stimulating hormone (ir-FSH) cells in pituitary glands of rats (Rattus norvegicus)in different age using immunohistochemical (IHC) methods. The pituitary organ that used in this study was collected from eight white female rats aged 2, 4, 6, and >12 months. Pituitary glands were processed histologically and stained with IHC methods to detect the ir-FSH cells. The result showed that the ir-FSH cells were observed in pars distalis (PD), pars tuberalis (PT), and pars intermedia (PI) of adenohypophysis with different number of cells. Furthermore, ir-FSH cells were found abundantly (+++) in the PT and a few (+) in PD of rats aged two months. In rats aged 4 months those cells were distributed in slight number (+) in PT and abundant (+++) in PD. In addition, the cells were also found in a great quantities in rats whose ages were 6 months, (+++) in both PT and PD, but these cells decreased (++) in rats aged >12 months in PD and absent (-) in PT. In conclusion, the devalopment and distribution of ir-FSH cells were found in pars tuberalis and pars distalis adenohypophysis in the pituitary glands of rats aged 2 up to >12 months with different number and distribution patterns.

20. The Histological Structure of Tegal Duck (Anas javanicus) Spleen at Different Age

Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Abstract

The aim of the  study was to determine the histological structure of tegal duck (Anas javanicus) spleen according to ages variation. One month, 2 month and 3 months of tegal duck were used as the sample of this study. The spleen of each duck was analyzed by Hematoxylin-Eosin (HE) method, then observed under light microscope. The result of this study performed there were the differences of histological structure of the ducks spleen. The capsule thickness had increased along with increasing age, which was 16.0 ± 1.0 µm; 25.3 ± 2.5 µm; and 29.0 ± 1.0 µm. The boundary between the two pulp in the tegal duck 3 months  old more obvious than 1 month or 2 months old. The diameter of white pulp had increased in the oldest duck. It can be concluded that the development of spleen histological structure was more perfect with the  increasing age of tegal duck.