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Bola Benih Sebagai Kiat Baru dalam Rehabilitasi Hutan dan Lahan Kritis di Kecamatan Tinggimoncong, Sulawesi Selatan Restu, Muh.; Millang, Syamsuddin; Paembonan, Samuel; Bachtiar, Budirman; Gusmiaty, Gusmiaty
Biocelebes Vol 6, No 2 (2012)
Publisher : Biocelebes

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Abstract

Rehabilitate forest and farm critical must special attention and need of the new method that more practical, quick, and cheap. Method of seed ball is its alternative method. with consideration referred, then this research bent on for (1) know seed amount every seed ball that give percentage grows and plants growth sengon the best (2) know size of seed ball diameter the best to percentage grow and plants growth sengon; (3) know appropriate type of farm closing to percentage grow and plants growth sengon; (4) know interaction influence between seed amount every ball, level of seed ball, type of the best farm closing to percentage grow and plants growth sengon at the site. This research is executed start month September 2012 up to final December 2012 in district Tinggimoncong Gowa Regency. this research uses random design complete pattern factorial that consisted of 3 factor that is seed amounts every seed ball as first factor with 4 levels that is 5 seeds, 7 seeds, 9 seeds, and 11 seeds every seed ball, second that is large size factor its diameter of seed ball that consisted of 4 levels that is 2.5 cm, 4 cm, 5.5 cm, and 7 cm, third factor that is farm closing that consisted of 4 levels that is opened (grass), coppice, beams, and cleaned (disc with diameter 20 cm). The three of this treatment factor are combined until got counted 4 x 4 x 4 = 64 treatment units. Every treatment unit consisted of 3 seed balls and repeated 3 times until required 64 x 3 x 3 = 576 seed balls. Variable that perceived is time starts germinate and percentage germinates every week during a month first, plants growth (high and leaf amount) every month after second month for 2 month. Research result indicates that treatment of land cover has an effect on reality to germination percentage, leaf amount, and high plants as for seedball diameter, seed amount and interaction between land cover and seedball diameter have an effect on reality to percentage of germination and leaf amount. Treatment of grass land cover gives influence is done well by germination percentage, leaf amount, and high plants. Seedball diameter 7,0 cm and seed amount 11 seeds give influence is done well by germination percentage. Combination of grass land cover treatment and diameter of seed ball 7,0 cm give best influence. Key words: Formulation, biological control agent, Aspergillus sp., and tablet.
KARAKTERISASI MORFOLOGI SUMBER BENIH TEGAKAN PINUS TERIDENTIFIKASI DI HUTAN PENDIDIKAN UNIVERSITAS HASANUDDIN Restu, Muh.; Gusmiaty, Gusmiaty
Biocelebes Vol 9, No 1 (2015)
Publisher : Biocelebes

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Abstract

This study aims  to investigate genetic diversity of identified pine stands at Unhas Extention Forest based on morphological markers.  The methods used are observation and measurement of the variables leaves, stems and plant height.  An analysis of data used the NTSYSpc program (Numerical Taxonomy and Multivariate System) version 2.0.The results showed that there are some morphological similarities such as  the shape of the leaves (needles), the shape of the curve of the leaf tip (tapered), the shape of the curve of the leaf base(tapering), the shape of the leaf margins (flat), the color of top surface of the leaf (dark green), the color of lower surface of leaf (dark green), the state of the upper surface of the leaf (smooth), the state of the lower leaf surface (rough), bark texture (grooved), rod shape (square), and the shape of the canopy (cones) while the other variables showed a difference. The genetic diversity of individual spine based on morphological observations is low.   Keywords  :  Morfological, Genetics,  Diversity, Pine
POLYMORPHISM OF SIMPLE SEQUENCE REPEAT REGIONS OF SULAWESI EBONY (DIOSPHYROS CELEBICA BAKH.) IN EXPERIMENTAL FOREST OF HASANUDDIN UNIVERSITY PROVENANCE Larekeng, Siti Halimah; Restu, Muh.; Gusmiaty, Gusmiaty; Rismawati, Rismawati
AgroTech Journal Vol 1, No 1 (2016): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

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Abstract

Polymerase Chain Reaction (PCR)-based molecular techniques have been used to detect the polymorphism in plants. The utilization of molecular markers plays essential role in germplasm characterization and plant breeding since the information of DNA marker technology can be exchanged between laboratories and should have standard method to be reproducible. The molecular aspect has been commonly linked to DNA isolation protocol and polymorphic molecular marker, thus can be used for molecular research recommendation purposes. The objectives of this study were to evaluate the capability of microsatellite marker of Ebenaceae Family for amplifying Ebony DNA, and to determine the appropriate PCR annealing temperatures. The DNA isolation of Ebony leaves from Experimental Forest of Hasanuddin University Provenance was carried out using Genomic DNA Mini Kit (Plant) Geneaid protocol. Nine of seventeen selected primers from the Genus Diospyros were able to amplify Ebony DNA. Amplification products produced polymorphic bands with different annealing temperatures (ranged from 53 to 56°C). These nine polymorphic primers will be recommended to use for future studies in genetic diversity as well as pollen dispersal pattern analyses.
RESPON KINETIN DAN TIPE EKSPLAN JABON MERAH (Antocephalus macrophyllus (Roxb.) Havil) SECARA IN VITRO Putriana, Putriana; Gusmiaty, Gusmiaty; M, Restu; Musriati, Musriati; N, Aida
BIOMA : JURNAL BIOLOGI MAKASSAR Vol 4, No 1 (2019)
Publisher : Departemen Biologi, Fakultas MIPA, UNHAS

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Keberhasilan kultur jaringan dipengaruhi oleh jenis atau tipe eksplan dan penambahan zat pengatur tumbuh. Kinetin (6-furfurylaminopurine) merupakan zat pengatur tumbuh golongan sitokinin yang banyak digunakan dalam kultur jaringan. Penelitian ini bertujuan mengetahui pengaruh penambahan kinetin dan tipe eksplan terhadap pembiakan in vitro jabon merah. Hasil penelitian menunjukkan bahwa kinetin 7 ppm memberikan pengaruh terbaik terhadap pertumbuhan daun dan akar. Tipe eksplan bagian pucuk merupakan bagian terbaik digunakan untuk pembiakan in vitro. Keyword : Antocephalus macrophyllus (Roxb.) Havil, kinetin, in vitro, tipe eksplan
Identifikasi dan Karakterisasi Mikoriza pada Tegakan Nyatoh (Palaquium sp.) Kurnia, Kurnia; Gusmiaty, Gusmiaty; Larekeng, Siti Halimah
PERENNIAL Vol 15, No 1 (2019)
Publisher : Forestry Faculty of Universitas Hasanuddin

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This research aimed to identify the mycorrhiza types that associated in roots and soil on  Palaquium sp. plant. This  research has implemented on December 2017 until February 2018. This research procedures made by two stages includes collecting soil and root samples on Palaquium sp plant on  Ko'mara Community Forest, Takalar District. Therefore, the second step is isolation, identification, and observing colonization of mycorrhiza spores in the Integrated Laboratory and Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Hasanuddin University, Makassar. The results showed types of spore that found were Glomus, Gigaspora and Acaulospora. Glomus spore has highest relative abundance in root samples whereas on soil samples were Glomus, Gigaspora, and Schleroderma with Glomus spores has highest relative abundance in. The colonization percentage on site was intermediate.