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SCREENING BAKTERI AMILOLITIK DAN SELULOLITIK DARI LIMBAH SAGU (Screening of Amylolytic and Cellulolytic Bacteria From Sago waste) Yanti, Nur Arfa; Munir, Asmawati
Jurnal BioWallacea Vol 1, No 1 (2014)
Publisher : Jurnal BioWallacea

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Abstract

Screening of indigenous bacteria from sago waste based on amylolytic and cellulolytic activity was done to obtain bacterial isolate having double activity, i.e. could to hydrolize of starch (amylolytic) and cellulose (cellulolytic). Screening amylolytic and cellulolytic bacteria was done based on amylolytic and cellulolytic activity on agar media. Determination of amylolytic activity on starch agar media was based on the presence of clear zone around the bacterial colony upon flooding with lugol’s iodine solution. Cellulolytic activity was determine based on the presence of clear zone around the bacterial colony on Carboxy methyl cellulose (CMC) agar upon flooding with congo red solution. Presence of a clear zone around the colony indicated starch and cellulose hydrolysis. The diameters of clear zone produced on CMC and starch agar were measured and used as an indication of the amylolytic and cellulolytic activities of the bacteria. The results of the screening based on amylolytic and cellulolytic activity showed that a number of 21 bacterial isolates that having both activities. LCA2 was the bacterial isolate with the highest amylolytic and cellulolytic activity as revealed by the size of clearing zone on both types of agar plates. The diameters of clear zone on starch and CMC agar were 4,98   and 3,65 cm2, respectively. Therefore, LCA2 isolate was bacterial isolate that potent for biconvertion sago hampas into value-added products. Keywords : Bacteria, Amylolytic, Cellulolytic, Sago waste.
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian; Muhiddin, Nurhayani H.
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB.Keywords : Production, PHB, Amylolytic bacteria, Sago starch.
Production of Poly--hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.322 KB)

Abstract

A new bacterial strain that produces amylase and poly-a-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics  and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by  Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.
Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.322 KB)

Abstract

A new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.
Acceleration of Paraquat Biodegradation by Isolated Soil Bacteria Martani, Erni; Yanti, Nur Arfa; Margino, Sebastian; Noegrohati, Sri
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 1 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.10105

Abstract

Herbicides residues were reported to have impact on the ecosystem. It was thought that acceleration of paraquat degradation would minimize these impacts. Paraquat was persistent in peat soil due to low pH. This study was done to investigate the role of bacterial isolates on the acceleration of paraquat degradation, especially in peat soil. The bacteria were isolated from several kinds of Indonesian soils using enrichment culture technique in a modified N-free medium. The medium was added with paraquat at gradually increase-concentration from 10, 20, and 40 ppm (w/w). Examinations in paraquat degradation were done in two levels. The first was in a synthetic medium (N-free medium); the second was in soil extract medium. Two kinds of peat were used to make the soil extract media, i.e. fibric and saphric peat soils.Several bacterial isolates were able to degrade paraquat in N-free medium. However, the degradation mode was different with those in peat soil extract media. None of them degraded paraquat in fibric and saphric soil extract media. It was suggested that the environmental limiting factors were responsible to the failure of paraquat degradation. Two selected isolates were able to degrade paraquat when the pH value of the extract medium was enhanced to around 5.5. Bacterial isolate of SM1, which was isolated from acid sulfate soil of Central Kalimantan, was the best isolate which was able to degrade paraquat in synthetic medium and peat soil extract media, especially in fibric extract medium. It degraded around 30%of paraquat within 15 day. Experiments are being done to enhance paraquat degradation by inoculation of mixed cultures of selected bacterial isolates.Key words: paraquat, degradation, bacterial isolates
PENGARUH PENAMBAHAN GULA DAN NITROGEN PADA PRODUKSI NATA DE COCO Yanti, Nur Arfa; Ahmad, Sitti Wirdhana; Tryaswaty, Desty; Nurhana, A.
Jurnal BioWallacea Vol 4, No 1 (2017): Biosains & Technology in Wallacea
Publisher : Jurnal BioWallacea

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Abstract

ABSTRAK Produk nata de coco merupakan makanan fungsional kaya serat yang dihasilkan oleh bakteri Acetobacter xylinum secara fermentasi menggunakan media air kelapa. Biosintesis nata de coco membutuhkan sumber karbon dan nitrogen. Penelitian ini bertujuan untuk mengetahui  konsentrasi gula dan sumber nitrogen, ZA yang terbaik untuk memproduksi nata de coco. Produksi nata de coco dilakukan dengan menambahkan gula dengan perlakuan konsentrasi 2; 3,5; 5 dan 7,5% (b/v), dan ZA  dengan perlakuan konsentrasi 0,2; 0,35; 0,5 dan 0,75% (b/v). Parameter yang diamati adalah ketebalan nata de coco yang diukur menggunakan jangka sorong dan rendemen nata. Hasil penelitian menunjukkan bahwa penambahan gula dan ZA pada media fermentasi berpengaruh terhadap produksi nata de coco. Konsentrasi gula 5% dan ZA 0,5%  yang terbaik menghasilkan nata de coco.  Kata Kunci : Nata de coco, Gula, Nitrogen, ZA.   ABSTRACT Nata de coco is a functional food  rich of fiber produce by Acetobacter xylinum bacteria by fermented using coconut water media. The biosynthesis of nata de coco requires a source of carbon and nitrogen. This study aims to determine is the best concentration of sugar and nitrogen source, ZA  for producing nata de coco. The production of nata de coco was done by adding sugar with the treatment of concentration 2; 3.5; 5 and 7.5% (w / v), and ZA with a treatment concentration of 0.2; 0.35; 0.5 and 0.75% (w/v). The observed parameters include the thickness of nata de coco which was measured using calipers and nata yield. The results showed that the addition of sugar and ZA in the fermentation media affect the production of nata de coco. The  concentration of sugar 5%  and ZA 0.5% is the best  to produce nata de coco . Keywords : Nata de coco, sugar, Nitrogen, ZA
Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.322 KB) | DOI: 10.22146/ijbiotech.7804

Abstract

A new bacterial strain that produces amylase and poly-a-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics  and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by  Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.
SCREENING BAKTERI AMILOLITIK DAN SELULOLITIK DARI LIMBAH SAGU (Screening of Amylolytic and Cellulolytic Bacteria From Sago waste) Yanti, Nur Arfa
REZ PUBLICA Vol 1, No 1 (2015): March - May
Publisher : Universitas Halu Oleo

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Abstract

ABSTRACTScreening of indigenous bacteria from sago waste based on amylolytic and cellulolytic activitywas done to obtain bacterial isolate having double activity, i.e. could to hydrolize of starch(amylolytic) and cellulose (cellulolytic). Screening amylolytic and cellulolytic bacteria wasdone based on amylolytic and cellulolytic activity on agar media. Determination of amylolyticactivity on starch agar media was based on the presence of clear zone around the bacterialcolony upon flooding with lugol’s iodine solution. Cellulolytic activity was determine based onthe presence of clear zone around the bacterial colony on Carboxy methyl cellulose (CMC)agar upon flooding with congo red solution. Presence of a clear zone around the colonyindicated starch and cellulose hydrolysis. The diameters of clear zone produced on CMC andstarch agar were measured and used as an indication of the amylolytic and cellulolyticactivities of the bacteria. The results of the screening based on amylolytic and cellulolyticactivity showed that a number of 21 bacterial isolates that having both activities. LCA2 was thebacterial isolate with the highest amylolytic and cellulolytic activity as revealed by the size ofclearing zone on both types of agar plates. The diameters of clear zone on starch and CMCagar were 4,98 and 3,65 cm2, respectively. Therefore, LCA2 isolate was bacterial isolate thatpotent for biconvertion sago hampas into value-added products
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian; Muhiddin, Nurhayani H.
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.882 KB) | DOI: 10.22146/ijbiotech.7877

Abstract

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB. Keywords : Production, PHB, Amylolytic bacteria, Sago starch.
Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.322 KB) | DOI: 10.22146/ijbiotech.7559

Abstract

A new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.