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Morfologi dan morfometrik perkembangan awal filosoma lobster mutiara (Panulirus ornatus)

Aquahayati Vol 9, No 1 (2013)
Publisher : Aquahayati

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Abstract

Telur lobster mutiara yang baru menetas dinamakan larva atau filosoma. Pengetahuan tentang filosoma dibutuhkan untukmengembangkan dan mengelola pembenihan. Penelitian ini bertujuan untuk mengeksplorasi morfologi dan morfometrikperkembangan awal filosoma lobster mutiara (Panulirus ornatus). Filosoma yang baru menetas dipelihara tanpa diberipakan. Hari ke 1, 3, 5, dan 7 hari setelah menetas, filosoma diamati morfologi dan morfometriknya dengan menggunakanmikroskop binokuler dan mikrometer. Hasil penelitian menunjukkan bahwa secara morfologi, tubuh dari filosoma tahapawal berbentuk datar, tidak berpigmen, transparan sehingga dari punggung atau perut, kontraksi jantung, usus, midgutgland terlihat jelas. Tubuh filosoma terdiri atas tiga bagian, yaitu: kepala, dada, dan pleon. Kepala terdiri atas ususbucccale sebagai bagian dari mulut, jantung, sepasang mata, antena, antenulla, maxilliped 1 , 2, dan 3, serta organ dalampenting lainnya. Di dada, terdapat organ pencernaan (semacam usus) dan 3 pereiopods. Periopod 1 dan 2 adalah exopod,dorsal coxal spine, plumose natatory setae, coxa, basis, ischio-merus,corpus dan dactylus. Ukuran tahap I adalah: panjangtubuh 1,431,47 mm (±0,05 mm), panjang cephala adalah 0,760,78 mm (±0,02 mm), dan lebar cephala adalah0,710,72 mm (±0,03 mm). Panjang Thorax adalah 0,460,48 mm (±0,08 mm), lebar dada adalah 0,360,38 mm (± 0,03mm), panjang pleon adalah 0,270,29 mm (±0,05 mm) dan lebar pleon adalah 0,11 mm (± 0,01 mm). Keadaan morfologidan morfometrik filosoma sampai hari ke 7 tidak mengalami perubahan, suatu indikasi bahwa filosoma lobster mutiaramempunyai perkembangan yang sangat lambat dan tetap berada pada stadia pertama.Kata kunci: lobster mutiara, Panulirus ornatus, morfologi, morfometrik, filosoma/larva

Perubahan Jaringan Hati Ikan Pari Kembang ( DASYATISKUHLI) Akibat Merkuri (HG)

TORANI : Jurnal Ilmu Kelautan dan Perikanan Vol 24, No 1 (2014)
Publisher : TORANI : Jurnal Ilmu Kelautan dan Perikanan

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Abstract

Blue Spotted Ray (Dasyatis kuhlii) as the demersal organisms has a lot of accumulation of pollutants in the water that settles to the bottom. This study was conducted to look at the liver tissue alteration of Blue Spotted Ray caused by contamination of mercury (Hg). Performed on 5 tanks. Tank A as a control, tank B with concentration 0.025  ppm,  tank  C  with  concentration  of  0.05  ppm,  tank  D  with concentration  0.1  ppm  and  tanks  E  with concentration  0.2  ppm.  The  result  is  alteration  on  the  liver  tissue  in  the  form  of  atrophy,  cloudy  swelling, vacuolization degeneration and even necrosis or death of liver tissue already. The alteration can reduce and eveneliminate the function of the liver as a toxin absorbent and can lead to death.

PEUBAH KUALITAS AIR YANG MEMPENGARUHI PERTUMBUHAN RUMPUT LAUT (Gracilaria verrucosa) DI TAMBAK TANAH SULFAT MASAM KECAMATAN ANGKONA KABUPATEN LUWU TIMUR PROVINSI SULAWESI SELATAN

Jurnal Riset Akuakultur Vol 4, No 1 (2009): (April 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

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Abstract

Rumput laut (Gracilaria verrucosa) telah dibudidayakan di tambak tanah sulfat masam dengan kualitas dan kuantitas produksi yang relatif tinggi. Oleh karena itu, dilakukan penelitian yang bertujuan untuk mengetahui peubah kualitas air yang mempengaruhi laju pertumbuhan rumput laut di tambak tanah sulfat masam Kecamatan Angkona Kabupaten Luwu Timur Provinsi Sulawesi Selatan. Pemeliharaan rumput laut dilakukan di 30 petak tambak  terpilih selama 6 minggu. Bibit rumput laut dengan bobot 100 g basah ditebar dalam hapa berukuran 1,0 m x 1,0 m x 1,2 m. Peubah tidak bebas yang diamati adalah laju pertumbuhan relatif, sedangkan peubah bebas adalah peubah kualitas air yang meliputi: intensitas cahaya, salinitas, suhu, pH, karbondioksida, nitrat, amonium, fosfat, dan besi. Analisis regresi berganda digunakan untuk menentukan peubah bebas yang dapat digunakan untuk memprediksi peubah tidak bebas. Hasil penelitian menunjukkan bahwa laju pertumbuhan relatif rumput laut di tambak tanah sulfat masam berkisar antara 1,52% dan 3,63%/hari dengan rata-rata 2,88% ± 0,56%/hari. Di antara 9 peubah kualitas air yang diamati ternyata hanya 5 peubah kualitas air yaitu: nitrat, salinitas, amonium, besi, dan fosfat yang mempengaruhi pertumbuhan rumput laut secara nyata. Untuk meningkatkan pertumbuhan rumput laut di tambak tanah sulfat masam Kecamatan Angkona Kabupaten Luwu Timur dapat dilakukan dengan pemberian pupuk yang mengandung nitrogen untuk meningkatkan kandungan amonium dan nitrat serta pemberian pupuk yang mengandung fosfor untuk meningkatkan kandungan fosfat sampai pada nilai tertentu, melakukan remediasi untuk menurunkan kandungan besi serta memelihara rumput laut pada salinitas air yang lebih tinggi, tetapi tidak melebihi 30 ppt.Seaweed (Gracilaria verrucosa) has been cultivated in acid sulfate soil-affected ponds with relatively high quality and quantity of seaweed production. A research has been conducted to study water quality variables that influence the growth of seaweed in acid sulfate soil-affected ponds of Angkona Sub-district East Luwu Regency South Sulawesi Province. Cultivation of seaweed was done for six weeks in 30 selected brackishwater ponds. Seeds of seaweed with weight of 100 g were stocked in hapa sized 1.0 m x 1.0 m x 1.2 m. Dependent variable that was observed was specific growth rate, whereas independent variables were water quality variables including light intensity, salinity, temperature, pH, carbondioxide, nitrate, ammonium, phosphate, and iron. Analyses of multiple regressions were used to determine the independent variables which could be used to predict the dependent variable. Research result indicated that relative growth rate of seaweed in acid sulfate soils-affected brackishwater ponds ranged from 1.52% to 3.63%/day with 2.88% ± 0.56%/day in average. Among nine observed water quality variables, only five variables namely: nitrate, salinity, ammonium, phosphate and iron influence significantly on the growth of seaweed in acid sulfate soils-affected brackishwater ponds. The growth of seaweed in acid sulfate soils-affected brackishwater ponds of Angkona District East Luwu Regency, can be improved by using nitrogen-based fertilizers to increase ammonium and nitrate contents and also fertilizers which contain phosphorus to improve phosphate content to a certain level. Pond remediation to decrease iron content and also rearing seaweed at higher salinity (but less than 30 ppt) can also be alternatives to increase the growth of seaweed.

Effectivity of mulberry leaf extract on stimulating ekdisteroid hemolimph content and molting of mud crab (Scylla olivacea)

TORANI: Journal of Fisheries and Marine Science Vol 2, No 1 (2018)
Publisher : Marine Science and Fisheries Faculty, Hasanuddin University

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Abstract

This study aims to study the performance of phytoecdisteroids from mulberry leaves in molting stimulating of mangrove crabs. The research was carried out at the Research and Development Center for Aquaculture at the Faculty of Marine Sciences and Fisheries at Hasanuddin University, located in Bojo Village, Mallusetasi District, Barru Regency, South Sulawesi Province. Mangrove crabs (Scylla olivacea) weighing 45-55 g and carapace widths 60-65 mm were used as test animals. There were five doses of mulberry leaf extract applied through feed, namely: A) 1.1 mg / g feed, B) 1.9 mg / g feed, C) 2.7 mg / g feed, D) 3.5 mg / g feed. Crabs were kept individually in plastic boxes that are floated above the surface of the pond. During rearing, crabs are fed 3% dry fish per day which has been enriched with mulberry leaf extract. The parameters observed were ecdysteroid compounds contained in mulberry leaf extract, ecdysteroid content in hemolymph before and after application of mulberry leaf extract, and molting percentage. Identification of the ekdisteroid compounds contained in mulberry leaf extract was carried out using Thin Layer Chromatography (TLC) and Measurement of the content of ecdysteroids in crab hemolymh was carried out using Ultra Fast Liquid Chromatography (UFLC).  The results showed that the 3.5 mg dose of mulberry leaf extract / g feed gave the highest increase in ecdysteroid hemolymph, which was approximately 1760 ekdisteroid per mL hemolimph, while the dose of 1.1 mg / g feed only provided an increase of 100 ng ekdisteroid per mL hemolymph. The higher the dose of mulberry leaf extract in the feed, the higher the increase in the concentration of hemolymph ecdysteroids, but the high concentration of ecdystoid in the hemolymph does not guarantee molting.  The optimal dose of mulberry leaf extract in the feed stimulating molting was 2.4 mg / g of feed.Keywords: mulberry, phytoecdysteroid, molting, crab, aquaculture

Pemanfaatan tepung testis sapi sebagai hormon alami pada penjantanan ikan cupang, Betta splendens Regan, 1910 [Cow’s testicles flour as the natural hormone masculinization of Siamese fighting fish, Betta splendens Regan, 1910]

Jurnal Iktiologi Indonesia Vol 16, No 1 (2016): Februari 2016
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

Siamese fighting fish, Betta splendens male is a lovely color ornamental fish with unique shape fins that make it highly demand by the ornamental fish lovers. This study aims to perform sex reversal with masculinization fish production. The study was carried out in two stages i.e.: stage 1 by soaking the 4 days old fish larvae into a solution of cow testicles flour with different doses, stage 2 with different soaking time. Testicular dose tested consists of five levels i.e.: 0 mg L', 20 mg L-1, 40 mg L-1, 60 mg L-1, and 80 mg L-1. Time immersions tested were: 0 hours, 24 hours, 36 hours, 48 hours and 60 hours. The measured parameter was the percentage of male fish produced. The results showed the highest per-centtage of male fish obtained at a dose of 60 mg L-1 and a 24-hour soaking time with a percentage value respectively 88.5% and 87.5%. The study provided information that masculinization technology in a solution of cow testicles applicable for fish larvae. This technology is easy to do so that farmers can use cow's testicles flour for masculinization for their fish production. AbstrakIkan Cupang, Betta splendens jantan merupakan ikan hias yang memiliki keindahan warna tubuh serta keunikan bentuk sirip sehingga sangat diminati oleh pecinta ikan hias. Penelitian ini bertujuan melakukan pembalikan kelamin dengan menjantankan ikan cupang yang diproduksi. Penelitian dilakukan dalam dua tahap yaitu: tahap pertama dengan meren-dam larva ikan cupang berumur empat hari ke dalam larutan tepung testis sapi dengan dosis berbeda, dan tahap ke dua dengan lama perendaman berbeda. Dosis testis yang diuji terdiri atas lima tingkatan yaitu 0 mg L-1, 20 mg L-1, 40 mgL-1 60 mg L-1, dan 80 mg L-1. Lama perendaman yang diuji adalah: 0 jam, 24 jam, 36 jam, 48 jam, dan 60 jam. Parameter yang diukur adalah persentase ikan jantan yang dihasilkan. Hasil penelitian menunjukkan bahwa persentase ikan berke-lamin jantan tertinggi diperoleh pada dosis 60 mg L-1 dan lama waktu perendaman 24 jam dengan nilai persentasi ber-turut-turut 88,5% dan 87,5%. Hasil penelitian ini memberikan informasi bahwa teknologi penjantanan melalui perendaman dalam larutan testis sapi dapat dilakukan pada larva ikan cupang. Teknologi ini mudah dilakukan sehingga pembudidaya dapat menggunakan tepung testis sapi untuk menjantankan ikan cupang produksinya.

Acceleration of Blue Swimming Crab (Portunus pelagicus) Larval Development by Phytoecdysteroid

Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

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Abstract

Phytoecdysteroid is a mimic hormone like molt regulating hormone in arthropoda and crustacea.  This research aims to investigate the response of Portunus pelagicus larvae on various doses of vitomolt enrichment in predigested artificial diet.  Four doses of vitomolt (0; 4; 40; 400 mg/100 g artificial diet) were used in this research.  Larval development and survival rate were measured The results showed that the larvae development rate was increased in congruent with the dose of vitomolt.  However, highest survival rate was found on 4 mg vitomolt/100 g artificial diet (P>0.05).  Based on regression analysis, it is estimated the optimal dose of vitomolt for larval development rate and survival rate was about 23 mg/100 g of artificial diet. Vitomolt application in artificial diet could be used to accelerate the larval development rate in blue swimming crab. Keywords: Portunus pelagicus, phytoecdysteroid, artificial diet, survival, larval development

The Morphometric Character and Mitochondrial 16S rRNA Sequence of Portunus pelagicus

Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

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Abstract

Blue swimming crab (Portunus pelagicus) colors have variations from different habitats and they have different vitality. The purpose of this study was to analyze the diversity of the P. pelagicus using morphometric and 16SrRNA mitochondrial sequence.  There were three groups of blue swimming crab characterized by differences in color and density patterns of spots.  The result of Canonical discriminant analysis showed that there were morphometric differences among the three groups.  The main distinguishing morphometric characteristics are the ratio of carapace width and length as well as the ratio of the length and width of cheliped merus.  The third group of crab is also has similarity about 98-99% by the Portunus pelagicus 16S rRNA sequences available in gene banks.  These studies had concluded that differences in color and pattern of the spot density have also morphometric and genetic differences. At least there were two groups based on genetic variation of Portunus pelagicus from Barru Regency waters which may have a variety of different properties.  Further research is needed to study the adaptation abilities of the two groups of crab on the condition of aquaculture as a potential domestic animal.Keywords: Crab; Portunus pelagicus; Morphometric; 16S rRNA.

Quality of Blue swimming crab Portunus pelagicus Larvae from Domesticated Broodstock

Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

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Abstract

Blue swimming crab (Portunus pelagicus) is the important world fishery resource, but the crab larval rearing faces high mortality problem. The aim of the research was to compare survival and growth rate of larvae resulted from wild and domesticated broodstock blue swimming crab. Domesticated and wild broodstock were used to produce larvae. Domesticated broodstock was selected from repeated reared crab until third generation. Selection of the broodstock mainly based on the survival and growth rate. The wild broodstock was collected from Makassar Strait Sea. Larvae from the two sources of broodstock were reared in the conical tanks with density of 50 zoeas per liter. After reaching megalopa phase, then, the larvae were transfered into concrete tank completed with shelter and it were reared until crab phase. Crab phase was reared for 18 days, then these seed were released into the brackishwater pond for further domestication step. Larva from zoea to crab were fed with rotifer, artemia, fresh small shrimp and diet. The result showed that domesticated broodstock produced higher survival rate (P<0.05) larvae, faster larval stage changes (P<0.01) and uniform larval growth compared to the larvae from wild broodstock. It is clearly shown, the use of domesticated broodstock of blue swimming crab produced better larva quality and quantity than the wild broodstoock crab.Keywords : blue swimming crab, larvae, domestication, broodstock selection, faster growth