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UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL RUMPUT LAUT (Eucheuma sp) PADA BERBAGAI TINGKAT KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus Hafizah, Indria; Akib, Nur Illiyin; Fajrianto, Muhammad
MEDULA Vol 1, No 2 (2014)
Publisher : Halu Oleo University

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Abstract

Red algae seaweed Eucheuma sp containflavonoids compound which has activity as anantibacterial. Thisstudyaimstodeterminethe difference of seaweed methanol extract(Eucheuma sp) on inhibiting the growth ofEscherichia coli andStaphylococcus aureusbacteria. This research was conducted with post–test only design (one-shot case study) with a variable treatment of theseaweed methanolextract (Eucheuma sp) against S. aureus and E. coli.Extractswerethendividedinto 5 concentration of 75%, 50%, 25%, 5%, and non-extraction (squeezed). Analysis of data t odetermine the level of concentration differences seen from the ANOVA test (analysis of variance) followedby a posthoc test. The results of bivariat analysis showed the inhibition of E. coli bacteria there are difference in clear zone diameter which are significant various levels of each concentration, with a value of p = 0.000(p < 0.05) and in S. Aureus inhibition P = 0.073 (p > 0.05) which means there is no difference in the diameter of clear zone at various levels for each concentration. The conclusions of this research are differences extract concentration have effect on the inhibition of the growth of the bacterium Escherichia coli and the difference in concentration of the extract have no effect on the inhibition of growth of Staphylococcus aureus.Key words : Eucheuma sp, Escherechia coli, Staphylococcus aureus
Pengaruh Minuman Tradisional Kameko Terhadap kadar SGOT, SGPT, dan Jaringan Hati Mencit (Mus musculus) Hafizah, Indria; Sudayasa, I Putu; Uddu, Waode Sitti Asfiah; Imran, Muhammad; Yakin, Aynul
Pharmauho Vol 3, No 1 (2017): Pharmauho
Publisher : Pharmauho

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Abstract

Penelitian ini bertujuan untuk melihat pengaruh minuman tradisional kameko terhadap kadar alanine transaminase (ALT), aspartate transaminase (AST), dan kerusakan jaringan hati mencit (Mus musculus). Mencit yang digunakan sebanyak 18 ekor masing – masing perlakuan terdiri atas 9 ekor yang dibagi atas 2 kelompok perlakuan yaitu kelompok etanol 2% dan kameko. Kelompok etanol 2% dan kameko diberi perlakuan selama 14 hari. Marka biokimia yang dilacak adalah kadar ALT dan AST  dalam serum serta perubahan histologi jaringan hati. Hasil penelitian menunjukkan bahwa kadar AST kelompok etanol 2% (1632 U/l) lebih tinggi di bandingkan kelompok kameko (1154 U/l), sementara kadar ALT lebih tinggi pada kelompok kameko (1263 U/l )  dibanding etanol 2% (1015 U/l). Pemberian etanol 2 % dan kameko mengakibatkan perubahan struktur mikroskopis (nekrosis) jaringan hati mencit dengan rasio AST/ALT > 0,8. Kerusakan jaringan hati akibat toksisitas dari etanol.Kata kunci: kameko, alanine transaminease, aspartate aminase,nekrosis
Antibacterial Activity of Eel (Anguilla spp.) Mucus against Salmonella typhi Nurtamin, Tomy; Nurman, Resty Yulianita; Hafizah, Indria
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

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Abstract

BACKGROUND: Typhoid fever has become one global health problem. Typhoid fever is caused by a Gram-negative bacterium, Salmonella typhi. Eel (Anguilla spp.) is a fish which lives in the sea or in freshwater. Several previous studies have found that Anguilla spp. mucus has the ability as antibacterial against Gram-positive and negative. Although the antibacterial activity of Anguilla spp. against various pathogens had been reported, very little is known about its activity against Salmonella typhi. The aim of this study was to investigate antibacterial activity of Anguilla spp. mucus against Salmonella typhi bacteria.METHODS: Present study was an experimental in vitro test. Antibacterial activity assays were carried out by the disc diffusion method. Antibacterial activity was determined by the clear zone formed around the paper disc and minimum inhibitory concentration was determined by observing the lowest concentration which could inhibit the growth of Salmonella typhi.RESULTS: Result of the present study showed that the Anguilla spp. mucus has inhibitory effects against Salmonella typhi. Minimum inhibitory concentration from the Anguilla spp. mucus was 12.5%.CONCLUSION: Anguilla spp. mucus has antibacterial activity against the Salmonella typhi bacteria.KEYWORDS: eel fish mucus, Anguilla spp., antibacterial activity, Salmonella typhi
Bioaktifitas Antibakteri Ekstrak Etanol Teripang Pasir (Holothuria scabra) terhadap Pertumbuhan Klebsiella pneumoniae Secara In Vitro Ervitha, Fifit; Sulastrianah, Sulastrianah; Hafizah, Indria
Medula Vol 3, No 1 (2015)
Publisher : Medula

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Abstract

Holothuria scabra is  one of the biota that commonly found in Southeast Sulawesi waters and traditionally use as a source food and medicine by local tribe. They also harvesting this species for exported to several countrywith high demand.  Even this species have commmercially beneficience, but there is lack of publication about the bioactivity, especially H. Scabra derived from southeast Sulawesi. This study aims to determine secunder metabolite that contain in the extractand to examine antibacterial bioactivity of H. scabra ethanol extract againstKlebsiellapneumoniae by defining the Minimum Inhibitory Consentration. The method using an experimental with post test onlycontrol group design. The extraction is using maseration method and the extract then  diluted and divided intoten concentration 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,78%, 0,39%, and 0,19%. The secunder metabolite was determined by using TLC and the MIC was determined by using dilution method. All consentration.As the result, TLC test showing positif result for alcaloid and triterpenoid and the nutrient broth starting to show no turbidity in concetration 0,78%. The conclusion of this study is ethanol extract of H. Scabra contain alkaloid and triterpenoid as secunder metabolite and the MIC was in 0,78% concentration.Keywords: Holothuria scabra, Klebsiella pneumoniae,  MIC, alkaloid, terpenoid
Aktivitas Antibakteri Ekstrak Etanol Porifera (Spongia Officinalis ) terhadap Staphylococcus Aureus ATCC 25923 Hafizah, Indria; Muliati, Fine Farhani; Sulastrianah, Sulastrianah
Medula Vol 4, No 1 (2016)
Publisher : Medula

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Abstract

Staphylococcus aureus is the most  dangerous of all of the many comman staphylococcal bacteria. It stains gram positive and is non moving small round shaped or non motile cocci..Porifera (Spongia officinalis) are lowly organised less evelved animals and can be used as the medication resources. The aim of this study was to inveftigate antibacterial extract etanol  of Porifera (Spongia officinalis)against the growth of Staphylococcus aureus ATCC 25923.Present study was an experimental in vitro test. Antibacterial activity assays were carried out by the disc diffusion method. Antibacterial activity was determined by the clear zone formed around the papaer disc and minimum inhibitor concentration which could inhibit the growth of Staphylococcus aureus ATCC 25923.The results are supported by the presence of secondary metabolite by phytochemical test such as alkaloid, terpenoid, steroid, saponin and tanin.Extract ethanolof Spongia officinalis. Extract etanol Spongia officinalishas antibacterial activity againts Staphylococcus aureus ATCC 25923. Minimum inhibitory concentration from the ekstrak etanol Spongia officinalis was 10 ppm.Conclution ; Extract etanol Spongia officinalishas antibacterial activity againts Staphylococcus aureus ATCC 25923Keywords: Spongia officinalis, Staphylococcus aureus, Antibacterial activities, MIC (Minimal Inhibitory Consentration)
UJI DAYA HAMBAT EKSTRAK DAUN TAPAK KUDA (Ipomoea pes caprae (L) R. Br. ) TERHADAP Staphylococcus aureus Alminsyah, Alminsyah; Hafizah, Indria; Sulastrianah, Sulastrianah
Medula Vol 2, No 1 (2014)
Publisher : Medula

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Abstract

Ipomoea pes caprae (L) R. Br are commonly used by the Indonesian’s people to treat wounds, ulcers and as antioxidant.  Based  on  previous  research  it  was  contains  terpenoids,  saponins, alkaloids,  tannins  and flavonoids. The aim of this study is to determine inhibition capacity of I. pes caprae (L) R. Br leaf extract toward Staphylococcus aureus isolated from patients infected wounds at a concentration of 100 % , 50 % , 25 % , 12.5 %  ,  6.25  %  ,  3.125  %  and  1.56  %. This  research  was  conducted  with  post-test  design  with  a  control  only. Erythromycin is  used as a control. One gram of  extract dissolved  in  10  ml  of  chloroform. Inhibition  zone  is measured  by  paper  disc  diffusion  method  on  Mueller  Hinton agar. Bivariat  analysis  showed  that there  is statistically significant differences of diameters of inhibition zone from each concentration (p=0,00) including control (p=0,00). Conclusion of this study are I. pes caprae (L) R. Br leaf chloroform extract have capacity to inhibit S. aureus growth and the inhibition respond increase with concentration. Keywords : Ipomoea pes caprae (L) R. Br, Inhibition test, Erythromycin, Staphylococcus aureus
Uji Daya Hambat Ekstrak Etanol Bintang Laut Bertanduk (Protoreaster nodosus) terhadap Bakteri Streptococcus sp. dan Candida albicans Hafizah, Indria; Sulastrianah, Sulastrianah
Medula Vol 3, No 1 (2015)
Publisher : Medula

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Abstract

Starfish is a marine biota that have potency for used in human health. Protoreaster nodosus is species of starfish that commonly found in water of southeast Sulawesi and not widely used by local community. The aim of this study is to find out bioactive compound and to examine the antibacterial and antifungal bioactivity ethanol exract of Protoreaster nodosus. Thin Layer Chromatography (TLC) is used in bioactive compound assay and disk diffussion method is used to examine antibacterial and antifungal activity. Ethanol extract of Protoreaster nodosus is diluted into 2000 ppm, 4000 ppm, 8000 ppm, 16.000ppm and 32.000 ppm. As the result result of this study, ethanol extract of Protoreaster nodosus are positively containing alkaloid, steroid, saponin, flavonoid and tannin. The diameter of clear zone of disk containing extract in nutrient agar with Streptococcus sp are  4,4 mm, 5,4 mm, 6,7 mm, 9,4 mm dan 11,4 mm, consecutively. The diameter of clear zone of disk containing extract in potato dextrose agar with Candida albicans are 6,6 mm, 9,15 mm, 11,5 mm, 13,95 mm and 20,7 mm, consecutively. In the conclusion, ethanol extract of Protoreaster nodosus from Southeast Sulawesi containing alkaloid, steroid, saponin, flavonoid and tannin. The Antibacterial activity is strong in concentration of 32.000 ppm and the antifungal activity is strong in 32.000  ppm, 16.000 ppm, 8.000 ppm, 4000 ppm concentration. Keywords: Protoreaster nodosus, antibacterial activity, antifungal activity Streptococcus sp, Candida albicans, secondary metabolite
HUBUNGAN INTENSITAS KEBISINGAN DENGAN PENINGKATAN TEKANAN DARAH KARYAWAN BANDARA HALUOLEO KENDARI Hafizah, Indria; Zamrud, Muhamad; Sudarman, Sudarman
Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Vol 1, No 1 (2017): Seminar Nasional Riset Kuantitatif Terapan 2017
Publisher : Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017

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Abstract

Kebisingan dan  yang melebihi  nilai ambang batas pada kurun waktu yang cukup lama akan berakibat pada perubahan frekuensi jantung, dan perubahan tekanan darah. Penelitian ini bertujuan untuk mengetahui gambaran intensitas kebisingan, perbandingan tekanan darah karyawan, dan hubungan intensitas kebisingan dengan peningkatan tekanan darah. Desain penelitian ini adalah Observasional analitik dengan pendekatan metode  cross sectional. Sampel terdiri dari 30 orang yang dipilih dengan menggunakan metode purposive sampling berdasarkan kriteria inklusi dan ekslusi dan dianalisis dengan uji wilcoxon untuk melihat perbedaan tekanan darah dan uji korelasi rank spearman untuk melihat hubungan intensitas kebisingan dengan peningkatan tekanan darah.Hasil penelitian menunjukkan bahwa adaunit kerja yang  memiliki intensitas kebisingan melebihi 85 dB. Terdapat perbedaan tekanan darah sebelum dan sesudah kerja dimana sistolik (p value = 0.000) dan diastolik (p value = 0.003). Terdapat hubungan yang signifikan antara intensitas kebisingan dengan peningkatan tekanan darah sistolik (p value = 0.000, r = 0.699), namun tidak signifikan untuk tekanan darah diastolik (p value = 0.102, r = 0.369).Kesimpulan penelitian ini terdapat  perbedaan secara signifikan (tekanan darah sistolik dan  diastolik) antara sebelum  dan  sesudah  bekerja  (terpapar  bising).  Ada  hubungan  signifikan  antara  tingkat  bising  dengan  peningkatan  tekanan darah sistolik dan diastolik pekerja . Pekerja disarankan untuk  selalu menggunakan alat penutup telinga saat melakukan pekerjaannya.Kata kunci— Kebisingan, Sistolik, Diastolik, Tekanan Darah.