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Niken Satuti Nur Handayani
Fakultas Biologi UGM

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Identifikasi Mutasi Gen β Globin Ekson 1 Pada Pembawa Thalassemia Satuti Nur Handayani, Niken; Tripramudya Onggo, Andika
Biogenesis Vol 2, No 1 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.633 KB)

Abstract

Thalassemia is an autosomal recessive genetic mutation disorder with symptoms similliar to anemia that causes deficiency synthesis of the globin chains (hemoglobin component inside erythrocytes). Thalassemia is classified based on secondary protein structure abnormalities in α globin protein or β globin protein. Based on data from Indonesian Thalassemia Foundation (YTI) in Indonesia, people with thalassemia, especialy β thalassemia is constantly increasing 8 to 10% per year, so it is very impotant to have a strategy that reduce the increasing number in the population. Genetic examination on the individual is very effective to detect quickly the person with thalassemia trait carrier, to reduce the number of thalassemia carrier population. Identification of 1st exon β globin gene mutations with sequencing method is one of the way to know the specific mutation in thalassemia carriers. Identification results can be used as a reference for the rapid detection of thalassemia trait carrier. This study aims to determine the type of mutation and location of nucleotide mutations in 1st exon β globin gene on carrier of thalassemia and the changes of amino acid translated by the mutant gene. The study was conducted by isolating the genome from the β thalassemia carrier blood, amplifying and sequencing the 1st exon β globin gene. The location of point mutation analysis and the changes of amino acid, was analyzed using computational method by comparative alignment using normal β globin gene as a comparison. The results of the research showed there is a point mutation in the 59th nucleotide caused by transition (T to C) and 147th nucleotide caused by transversion (G to C). The mutation type found on this study was a silent mutation because there is no change in the translated amino acid.Keywords: β globin, exon 1, mutation, thalassemia
Karakter Morfologis dan Molekular Macrobrachium spp. dari Sungai Opak Daerah Istimewa Yogyakarta T, Trijoko; Satuti Nur Handayani, Niken; Widianawati, Anita; Eprilurahman, Rury
Biogenesis Vol 3, No 1 (2015)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.686 KB)

Abstract

Several rivers flow across Daerah Istimewa Yogyakarta (DIY includes Opak River which it’s upstream located on Merapi Volcano area. The river has a lot of biological resources, includes prawns. Freshwater prawns (Macrobrachium spp.) has important role in maintaining the balance of freshwater ecosystems. The purpose of this research was to determine species diversity of Macrobrachium spp. and to understand the morphological and molecular characters of them. Phenetic similarity was analyzed using 74 morphological characters, ISSR1 and ISSR3 primers. The results were analyzed using a program MVSP 3.1 (Multi- Variate Statistical Package) to obtain a dendogram that showed both intra-and interspecies similarity of Macrobrachium spp. Six species of Genus Macrobrachium were identified, they are Macrobrachium pilimanus, M. sintangense, M. lanchesteri, M. cowlesi, M. latidactylus, and M. equidens. Both morphological and molecular analyses show two major clusters with the similarity of 55.6% for morphology and 21.52% for molecular. Based on morphological and molecular characters analysis, we concluded that phenetic similarity of Macrobrachium spp. in Opak River was affected by the morphological structure of the second pereiopod, habitat type, size and number of eggs, as well as the type of larval development.Keywords: characters, Macrobrachium, molecular, morphology, Opak River
Keragaman Nukleotida Gen Lcy-b (Lycopene beta cyclase) Kultivar Tomat Betavila F1, Fortuna F1 dan Tymoti F1 Thoifur Ibnu Fajar, Muhammad; P, Purnomo; Satuti Nur Handayani, Niken
Biogenesis: Jurnal Ilmiah Biologi Vol 4, No 2 (2016)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1053.428 KB) | DOI: 10.24252/bio.v4i2.2508

Abstract

Tomato has three lycopene gene, namely lycopene beta cyclase (Lcy-b), lycopene beta-cyclase kromoplas (Cyc-b) and lycopene epsilon cyclase (Lcy-e). Lcy-b gene can be used as determinant of phylogenetic relationship between tomato and chili. This research aims to know phylogenetic relationship among tomatoes cultivar, namely Betavila F1, Fortuna F1 and Tymoti F1, then phylogenetic relationship between these tomatoes cultivar and tomatoes sequences Kristin KC140137.1, Darsirius KC140135.1, Pennellii XM 015217853.1, Villosum KP313876.1 and outgroup Capsicum annuum GU085266.1. Besides that, this research also aims to know the difference of nucleotide among tomatoes cultivar and the others tomato sequences and the outgroup Capsicum annuum based of alignment result. PCR (Polymerase Chain Reaction) method and NCBI primer design used to isolate Lcy-b gene. Acquired sequences were analyzed by Mega 6 and Clustal X softwares. The result of phylogenetic relationship among tomatoes cultivar showed Betavila F1 cultivar closely related to Tymoti F1 cultivar and Fortuna F1 cultivar closely related to Tymoti F1 cultivar. Phylogenetic relationship between tomatoes cultivar and the other tomatoes sequences showed Fortuna F1 cultivar closely related to Darsirius cultivar and varieties Pennellii, Betavila F1 cultivar distantly related to the other tomatoes sequences, and Tymoti F1 cultivar closely related to Kristin cultivar. The difference of nucleotide were found at Pennellii tomato that is 1 nucleotide with transition mutation, found at Villosum tomato that are 12 nucleotides with transition and transversion mutation, and found at Capsicum annuum that are 16 nucleotides with transition and transversion mutation. Phylogenetic relationship using Lcy-b gene expected to be used as a transgenic strategy to modify carotenoid content that improves tomatoes nutrition
Analisis Pedigree dan Fenotip Pasangan Kembar: Studi Kasus Pada Keluarga Kembar di Kecamatan Laweyan, Surakarta Machwiyah, Yuyun; Satuti Nur Handayani, Niken
Biogenesis: Jurnal Ilmiah Biologi Vol 1, No 1 (2013)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1629.715 KB) | DOI: 10.24252/bio.v1i1.443

Abstract

The twin pregnancy is a kind of pregnancy with two or more fetuses. Basically, the twins can be grouped of two, they are fraternal twins (dizygotic) and identical twins (monozygotic). Fraternal twin is a kind of twins that comes from two ova, which each of them will be fertilized by the sperm. While, identical twin comes from an ovum, which fertilized by a sperm. This research is conducted in order to observe the inheritance pattern from the pedigree chart, and to observe similarity and dissimilarity phenotype between twin couple on families observed. Methods used for this research are data collection to construct pedigree, phenotype analysis to compare the similarity and dissimilarity phenotype between the twins that are observed, paraffin method to observe the form of hair from cross section and agglutination reaction to observe the blood group from the twins. Based on pedigree chart of twins in Laweyan, Surakarta, it seems that the identical twins is passedfrom generation to generation, and on the family observed the inheritance pattern of identical twins birth process is come from the women of identical twins. The lobe of ear from each twins there is a twin couple showing the different lobe. There is any similarity and dissimilarity relate to form of hair cross section from each couple. The blood group of each couple are showing the same result.Keywords: identical twins, inheritance pattern, pedigree chart
Karakter Morfologis dan Molekular Macrobrachium spp. dari Sungai Opak Daerah Istimewa Yogyakarta T, Trijoko; Satuti Nur Handayani, Niken; Widianawati, Anita; Eprilurahman, Rury
Biogenesis: Jurnal Ilmiah Biologi Vol 3, No 1 (2015)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.686 KB) | DOI: 10.24252/bio.v3i1.559

Abstract

Several rivers flow across Daerah Istimewa Yogyakarta (DIY includes Opak River which it’s upstream located on Merapi Volcano area. The river has a lot of biological resources, includes prawns. Freshwater prawns (Macrobrachium spp.) has important role in maintaining the balance of freshwater ecosystems. The purpose of this research was to determine species diversity of Macrobrachium spp. and to understand the morphological and molecular characters of them. Phenetic similarity was analyzed using 74 morphological characters, ISSR1 and ISSR3 primers. The results were analyzed using a program MVSP 3.1 (Multi- Variate Statistical Package) to obtain a dendogram that showed both intra-and interspecies similarity of Macrobrachium spp. Six species of Genus Macrobrachium were identified, they are Macrobrachium pilimanus, M. sintangense, M. lanchesteri, M. cowlesi, M. latidactylus, and M. equidens. Both morphological and molecular analyses show two major clusters with the similarity of 55.6% for morphology and 21.52% for molecular. Based on morphological and molecular characters analysis, we concluded that phenetic similarity of Macrobrachium spp. in Opak River was affected by the morphological structure of the second pereiopod, habitat type, size and number of eggs, as well as the type of larval development.Keywords: characters, Macrobrachium, molecular, morphology, Opak River
Identifikasi Mutasi Gen β Globin Ekson 1 Pada Pembawa Thalassemia Satuti Nur Handayani, Niken; Tripramudya Onggo, Andika
Biogenesis: Jurnal Ilmiah Biologi Vol 2, No 1 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.633 KB) | DOI: 10.24252/bio.v2i1.469

Abstract

Thalassemia is an autosomal recessive genetic mutation disorder with symptoms similliar to anemia that causes deficiency synthesis of the globin chains (hemoglobin component inside erythrocytes). Thalassemia is classified based on secondary protein structure abnormalities in α globin protein or β globin protein. Based on data from Indonesian Thalassemia Foundation (YTI) in Indonesia, people with thalassemia, especialy β thalassemia is constantly increasing 8 to 10% per year, so it is very impotant to have a strategy that reduce the increasing number in the population. Genetic examination on the individual is very effective to detect quickly the person with thalassemia trait carrier, to reduce the number of thalassemia carrier population. Identification of 1st exon β globin gene mutations with sequencing method is one of the way to know the specific mutation in thalassemia carriers. Identification results can be used as a reference for the rapid detection of thalassemia trait carrier. This study aims to determine the type of mutation and location of nucleotide mutations in 1st exon β globin gene on carrier of thalassemia and the changes of amino acid translated by the mutant gene. The study was conducted by isolating the genome from the β thalassemia carrier blood, amplifying and sequencing the 1st exon β globin gene. The location of point mutation analysis and the changes of amino acid, was analyzed using computational method by comparative alignment using normal β globin gene as a comparison. The results of the research showed there is a point mutation in the 59th nucleotide caused by transition (T to C) and 147th nucleotide caused by transversion (G to C). The mutation type found on this study was a silent mutation because there is no change in the translated amino acid.Keywords: β globin, exon 1, mutation, thalassemia
Karakterisasi Kromosom Stroberi (Fragaria vesca L. subsp. californica Cham. & Schltdl. cv. Californica) Hasil Poliploidisasi Khoiroh, Rosyidatul; Riza Aristya, Ganies; S, Sutikno; Satuti Nur Handayani, Niken
Biogenesis: Jurnal Ilmiah Biologi Vol 3, No 2 (2015)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.332 KB) | DOI: 10.24252/bio.v3i2.932

Abstract

Fruit consumption such as strawberries always increase in Indonesia and reaching about 210 tons in 2011. Strawberry (Fragaria vesca L. subsp. californica Cham. and Schltdl. cv. Californica) has been developed in Indonesia, especially in Banyuroto, Sawangan, the district of Magelang, Central Java. In order to improve the fruit productivity (quality and quantity), geneticcharacterization including chromosomes character of this fruit are needed to be investigate. Therefore, the research was conducted to determine the chromosomes number, chromosomes size chromosomes shape and karyotype of control and strawberries treated by colchicine as mutagenic agent. The method was squash and flow cytometry method. Whereas the cell suspension samplewere observed used flow cytometer instrument. The result of research shown that the chromosome number of strawberry Californica in normal and treatment strawberries was 2n=14. Thechromosomes shape of normal strawberry was 10 metacentric and 4 submetacentric. Whereas the chromosomes shape of treatment strawberries used colchicine was 10 metacentric and 4submetacentric. Flow cytometric analysis shown that control strawberries and treated strawberries has diploid ploidy level.Keywords: chromosomes, colchicine, flow cytometry, polyploidization, strawberry Californica
Karakter Morfologis dan Molekular Macrobrachium spp. dari Sungai Opak Daerah Istimewa Yogyakarta T, Trijoko; Satuti Nur Handayani, Niken; Widianawati, Anita; Eprilurahman, Rury
Biogenesis: Jurnal Ilmiah Biologi Vol 3, No 1 (2015)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i1.558

Abstract

Several rivers flow across Daerah Istimewa Yogyakarta (DIY includes Opak River which it’s upstream located on Merapi Volcano area. The river has a lot of biological resources, includes prawns. Freshwater prawns (Macrobrachium spp.) has important role in maintaining the balance of freshwater ecosystems. The purpose of this research was to determine species diversity of Macrobrachium spp. and to understand the morphological and molecular characters of them. Phenetic similarity was analyzed using 74 morphological characters, ISSR1 and ISSR3 primers. The results were analyzed using a program MVSP 3.1 (Multi- Variate Statistical Package) to obtain a dendogram that showed both intra-and interspecies similarity of Macrobrachium spp. Six species of Genus Macrobrachium were identified, they are Macrobrachium pilimanus, M. sintangense, M. lanchesteri, M. cowlesi, M. latidactylus, and M. equidens. Both morphological and molecular analyses show two major clusters with the similarity of 55.6% for morphology and 21.52% for molecular. Based on morphological and molecular characters analysis, we concluded that phenetic similarity of Macrobrachium spp. in Opak River was affected by the morphological structure of the second pereiopod, habitat type, size and number of eggs, as well as the type of larval development.Keywords: characters, Macrobrachium, molecular, morphology, Opak River
Keragaman Nukleotida Gen Lcy-b (Lycopene beta cyclase) Kultivar Tomat Betavila F1, Fortuna F1 dan Tymoti F1 Thoifur Ibnu Fajar, Muhammad; P, Purnomo; Satuti Nur Handayani, Niken
Biogenesis: Jurnal Ilmiah Biologi Vol 4, No 1 (2016)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v4i1.2506

Abstract

Tomato has three lycopene gene, namely lycopene beta cyclase (Lcy-b), lycopene beta-cyclase kromoplas (Cyc-b) and lycopene epsilon cyclase (Lcy-e). Lcy-b gene can be used as determinant of phylogenetic relationship between tomato and chili. This research aims to know phylogenetic relationship among tomatoes cultivar, namely Betavila F1, Fortuna F1 and Tymoti F1, then phylogenetic relationship between these tomatoes cultivar and tomatoes sequences Kristin KC140137.1, Darsirius KC140135.1, Pennellii XM 015217853.1, Villosum KP313876.1 and outgroup Capsicum annuum GU085266.1. Besides that, this research also aims to know the difference of nucleotide among tomatoes cultivar and the others tomato sequences and the outgroup Capsicum annuum based of alignment result. PCR (Polymerase Chain Reaction) method and NCBI primer design used to isolate Lcy-b gene. Acquired sequences were analyzed by Mega 6 and Clustal X softwares. The result of phylogenetic relationship among tomatoes cultivar showed Betavila F1 cultivar closely related to Tymoti F1 cultivar and Fortuna F1 cultivar closely related to Tymoti F1 cultivar. Phylogenetic relationship between tomatoes cultivar and the other tomatoes sequences showed Fortuna F1 cultivar closely related to Darsirius cultivar and varieties Pennellii, Betavila F1 cultivar distantly related to the other tomatoes sequences, and Tymoti F1 cultivar closely related to Kristin cultivar. The difference of nucleotide were found at Pennellii tomato that is 1 nucleotide with transition mutation, found at Villosum tomato that are 12 nucleotides with transition and transversion mutation, and found at Capsicum annuum that are 16 nucleotides with transition and transversion mutation. Phylogenetic relationship using Lcy-b gene expected to be used as a transgenic strategy to modify carotenoid content that improves tomatoes nutrition.