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Maria Marina Herawati
Satya Wacana Christian University

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INDUKSI KALUS DAN PROLIFERASI ARTEMISIA CINA BERG EX POLJAKOV Herawati, Maria Marina; Purwantoro, Aziz; Sulistyaningsih, Endang; Pramono, Suwijiyo
Agric Vol 26 No 1 (2014)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.451 KB) | DOI: 10.24246/agric.2014.v26.i1.p45-51

Abstract

Penelitian ini bertujuan untuk mengevaluasi efek kondisi terang dan gelap pada induksi dan proliferasi kalus dari kalus Artemisia cina. Eksplan steril akan dikulturkan pada media MS dengan kandungan 2.4-D 1 mg/L dan diletakkan pada kondisi terang dan gelap. Hasil penelitian menunjukkan bahwa induksi kalus akan lebih efektif pada kondisi gelap, sedangkan proliferasi kalus lebih efektif pada kondisi terang. Tahap terbaik untuk proses regenerasi adalah hari ke-48 setelah transfer dari media induksi.
SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI in vitro EKSTRAK HEKSANA-PETROLEUM ETER Artemisia cina Berg. ex Poljakov Kristiani, Elizabeth B.E; Kasmiyati, Sri; Herawati, Maria Marina
Agric Vol 27 No 1 (2015)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.296 KB) | DOI: 10.24246/agric.2015.v27.i1.p30-37

Abstract

The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.