Teni Ernawati
Research Center for Chemistry - Indonesian Institute of Sciences (LIPI), Kawasan Puspiptek Serpong, Tangerang Selatan 15314

Published : 12 Documents
Articles

Found 12 Documents
Search

Molecular Docking Simulation of Cinchona Alkaloids Derivatives as Anti-cancer Agent Ernawati, Teni
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 1, No. 2 (2019)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463 KB) | DOI: 10.24198/ijpst.v1i2.20203

Abstract

One of the triggers of breast cancer is the estrogen hormone. Estrogen α receptors play a role in breast development and growth. Estrogen receptors as molecular targets have been widely reported. Cinchona alkaloids have been known to function as anti-malarial, but recent studies have shown that cinchona alkaloids have other potentially such as anti-cancer, anti-tumor, anti-microbial, anti-HBV, anti-inflammatory, anti-oxidant, anti-obesity. Cinchona alkaloids have been developed as anti-cancer agent. They have comparable reactivity and selectivity of cinchona alkaloids functional to anti-cancer agent. In this study, molecular modeling has been performed on forty-four cinchona alkaloid derivatives. These cinchona alkaloids derived compounds have been evaluated as anti-cancer agent. Herein, we observed molecular interactions between selectivity of cinchona alkaloids with estrogen α receptors (ER-α). The docking simulation showed 3 cinchona alkaloids derivatives (cinchonine 2-chlorobenzoate, cinchonidine benzoate and cinchonine 2-(4-hydroxyphenyl) acetate) have lower free energy Gibs and and low kinetic inhibition compared to tamoxifen (as standard commercial) and there are 14 compounds that have relatively the same activity as tamoxifen from 44 alkaloid chincona derivatives.Keywords: cinchona alkaloids derivatives, quinine, quinidine, cinchonine, cinchonidine, anti-cancer, molecular docking
Cinnamic Acid Derivatives as α-Glucosidase Inhibitor Agents Ernawati, Teni; Radji, Maksum; Hanafi, Muhammad; Mun’im, Abdul; Yanuar, Arry
Indonesian Journal of Chemistry Vol 17, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.582 KB) | DOI: 10.22146/ijc.23572

Abstract

This paper reviews biological activity of some cinnamic acid derivative compounds which are isolated from natural materials and synthesized from the chemical compounds as an agent of α-glucosidase inhibitors for the antidiabetic drug. Aegeline, anhydroaegeline and aeglinoside B are natural products isolated compounds that have potential as an α-glucosidase inhibitor. Meanwhile, α-glucosidase inhibitor class of derivatives of cinnamic acid synthesized compounds are p-methoxy cinnamic acid and p-methoxyethyl cinnamate. Chemically, cinnamic acid has three main functional groups: first is the substitution of the phenyl group, second is the additive reaction into the α-β unsaturated, and third is the chemical reaction with carboxylic acid functional groups. The synthesis and modification of the structure of cinnamic acid are very influential in inhibitory activity against α-glucosidase.
Sintesis N-Oktilsinamamid dan Aktivitasnya terhadap Sitotoksik Sel Kanker Leukemia P388 Ernawati, Teni; Nurhalimah, Neneng; Minarti, Minarti
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 3, No. 2, November 2017
Publisher : Department of Chemistry Faculty of Sciences and Technology UIN Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.15408/jkv.v0i0.5843

Abstract

Sintesis senyawa N-Oktilsinnamamid yang diturunkan dari senyawa metil trans-sinamat dengan menggunakan katalis basa telah dilakukan. Senyawa metil sinamat terlebih dahulu dikonversi menjadi asam sinamat melalui reaksi hidrolisis dengan basa menghasilkan asam sinamat. Selanjutnya asam sinamat diamidasi dengan menggunakan oktilamin dan 1,3 disikloheksilkarboodiimide (DCC) dan 4-dimetilaminopiridin (DMAP) sebagai katalis. Dari penelitian ini diperoleh rendemen hasil sintesis asam sinamat, sintesis N-Oktilsinnamami yang cukup baik. Hasil reaksi diidentifikasi dengan menggunakan 1H-NMR, 13C-NMR dan LC-MS. Uji sitotoksik senyawa N-Oktilsinamamid terhadap sel leukemia P388 diperoleh nilai IC50=6.71 µg/mL. Synthesis of N-Octylcinnamamide compound derived from methyl trans-cinnamate using abase catalyst has been done. First, The compound of methyl trans-cinnamate was converted into cinnamic acid by hydrolysis reaction with an alkaline condition. Furthermore cinnamic acid was amidated by using octylamine and 1,3 dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) as a catalyst. The results of this study were obtained synthesis of cinnamic and synthesis of N-Octylcinnamamide are good enough. Identification of this product was using by 1H-NMR, 13C-NMR and LC-MS.  Cytotoxic test of N-Octylcinnamamide against P388 leukemia cell was obtained IC50=6.71µg/mL.
PENAPISAN VIRTUAL SENYAWA TURUNAN METIL SINAMAT PADA ENZIM SIKLOOKSIGENASE-2 (COX-2 Ernawati, Teni
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 14, No 2 (2012)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.69 KB) | DOI: 10.14203/jkti.v14i2.350

Abstract

Inflammation is the response of living tissue to vascular injury. Non-Steroid Anti-Inflammatory Drugs(NSAIDs) could control pain caused by inflammation. Therapeutic effects of NSAIDs are related to themechanism of the cyclooxygenase-1 (COX-1) enzyme and the cyclooxygenase-2 (COX-2) enzyme in inhibiting theproduction of prostaglandins. In this paper, we report the virtual screening of methyl cinnamate derivativecompounds against the COX-2. All methyl cinnamate derivative compounds were molecular docking simulatedusing the Protein-Ligand ANT System (PLANTS) software with ZINC03814717 as the reference compound. Fromthe results of virtual screening, it was obtained that derivative compounds of methyl cinnamate were predicted asactive COX-2 inhibitors because they have ChemPLP score better than that of the reference compoundsZINC03814717. The compound of acid 3- (4-benzoylphenyl) propionate was predicted as having the best score ofChemPLP.Keywords: Non-Steroid Anti-Inflammatory (NSAID), virtual screening, enzyme cyclooxygenase-2 (COX-2), methyl cinnamate derivative
Synthesis Methyl Nitrophenyl Acrylate and Cytotoxic Activity Test against P388 Leukemia Cells Ernawati, Teni; Khoirunni’mah, Zulfa
Indonesian Journal of Chemistry Vol 15, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.357 KB) | DOI: 10.22146/ijc.21226

Abstract

Synthesis of methyl nitrophenyl acrylate via modification of methyl trans-cinnamate had been done to improve its biological activity. The reaction of methyl trans-cinnamate with nitrating agent gave methyl 3-(2-nitrophenyl)acrylate and methyl 3-(4-nitrophenyl)acrylate with an ortho/para ratio of 1:8. Its structure was confirmed with 1H-NMR, 13C-NMR, FTIR, GC-MS. Biological activity of methyl 3-(4-nitrophenyl)acrylate and methyl 3-(2-nitrophenyl)acrylate assays was performed on Cancer cells against P388 Murine Leukemia with IC50= 7.98 μg/mL, IC50 = 27.78 μg/mL.
PENGARUH KOMPOSISI HERBAL DALAM MINUMAN TEH HIJAU GAMBUNG HERBAL TERHADAP HAMBATAN α-GLUKOSIDASE Megawati, Megawati; Mulyani, Hani; Minarti, Minarti; Ernawati, Teni; Darmawan, Akhmad
Jurnal Ilmiah As-Syifaa Vol 10, No 2 (2018): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.017 KB)

Abstract

The aim of this study is to make a formula of green tea that is enriched by other herbs. Green tea drinks are made using the basic ingredients of gambung (THG) green tea enriched with sapodilla leaves (Manilkara zapota (Aw) bay leaves (Syzygium polyanthum (Am), Artocarpus communis (Un) breadfruit leaves, and cinnamon leaves (Cinnamomum verum (K)). The composition of gambung herbal green tea is made by reference to the antidiabetic activity of each herb. All the samples were tested for inhibition of α-glucosidase enzymes and toxicity using the lethality test method for brine shrimp. All samples showed good inhibition of the α-glucosidase enzyme barriers of gamboeng green tea (THG) 82.05%, sapodilla leaves (Aw) 97.81%, breadfruit leaves (Un) 73.34%, bay leaves (Am) 93.80%, cinnamon leaves (K) 91.24 %. The inhibition value of α-glucoside enzymes in the formulation of gamboeng herbal green tea drinks: 96.203% (GKUn) is higher when compared to the GKAm formula (94.78%) and GKAw (62.487%).
STANDARDISASI EKSTRAK PEGAGAN, CENTELLA ASIATICA SEBAGAI OBAT HERBAL TERSTANDAR HEPATOPROTEKTOR Lotulung, Puspa Dewi N; Handayani, Sri; Ernawati, Teni; Yuliani, Tri; Artanti, Nina; Mozef, Tjandrawati
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.939 KB) | DOI: 10.14203/jkti.v17i2.34

Abstract

Herbal medicinal products would be affected by the quality of raw materials. In turn, the quality of raw material will also be influenced by various factors such as soil conditions, cultivation, post-harvest processing, and the processing of raw materials into crude drug or extract. Therefore, in order to make good herbal medicines, it is necessary to make standardization of herbal extracts that produced herbal medicines that have the same quality and functions of effectiveness in each process. From preliminary studies that have been done, Centella asiatica is one of the potential plants as a source of hepatoprotective compounds. Test in vivo and in vitro against Centella asiatica extracts have shown very good results. Ethyl acetate extract with 17.5 mg/kg of doses body weight and butanol 228.8 mg/kgof doses body weight has been applied for in vivo test using mice induced by CCl4; theydemonstrated hepatoprotective effects. Ethyl acetate extracts were able to reduce levels of the enzyme alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by 56 % and 44 % respectively while butanol extract can reduce the enzymes AST levels by 3%. Standardizationof Centella asiatica extract performed in this study was the characterization of the extract in the form of non-specific and specific parameters corresponding to the reference of PPOMN (Ministry of health Republic of Indonesia, 2000) such as levels of drying shrinkage, ash content, total plate count microbial contamination, levels of water-soluble compounds, levels of compounds that are soluble in ethanol, phytochemical test, total phenolic content, total flavonoid content and the determination of Pb and Cd weight.The results showed that non-specific parameters for the ethanol extract of Centella asiatica were requirements based on Herbal Pharmacopoeia in 2008 which includes parameters such as determination of shrinkage on drying ≤ 10%, ash content ≤ 16.6% and negative microbial contamination. Specific parameters for the ethanol extract of Centella asiatica have met the requirements of Herbal pharmacopeia in 2008.Keywords: Centellaasiatica, hepatoprotective, standardized herbal medicine, specificparameters, and non-specific parameters
Synthesis, Characterization, Anticancer and In Silico ADME Properties of Caproic Acid Derivatives against P388 Cancer Cell Lines Widiyarti, Galuh; Sundowo, Andini; Megawati, Megawati; Ernawati, Teni
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 1, No. 2 (2019)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.563 KB) | DOI: 10.24198/ijpst.v1i2.20192

Abstract

The in-silico properties of absorption, distribution, metabolism, and excretion (ADME) and drugs likeness of caproic acid ester derivativeswere evaluated. The esterification of caproic acid from palm kernel oil with sitronelol and geraniol from citronella oil has been carried out using base (NaOH) catalyst. The cytotoxicity of ester compounds were also tested against P388 murine leukemia cancer cell lines using MTT method. Analysis of the ester productswere carried outusing spectroscopic (IR, MS and 1H-NMR) methods, which were confirmed that the desired compounds were successfully synthesized. Caproic acid ester derivatives showed higher anticancer activity compared to the parent caproic acid for P388 cancer cell lines. Citronellyl caproate with IC50 4.15x10-2µM has the strongeractivity than geranyl caproate with IC50 11x10-2 µM.  ADME studies for the synthesized compounds revealed that the ester compounds have fulfill the Lipinski rule of five and has no carcinogenicity effect to rat or mouse.Key words: caproic acid, ester, P388, MTT, ADME
Sintesis Fenil Sinamat dan 4-Fenilkroman-2-on dan Uji Sitotoksisitas Terhadap Sel Kanker Serviks HeLa ERNAWATI, TENI; FAIRUSI, DILA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.433 KB)

Abstract

Asam sinamat merupakan turunan dari metil sinamat, yang termasuk dalam jalur turunan asam shikimat. Asam sinamat dan analog alaminya dikenal dalam pengobatan kanker selama beberapa abad. Sintesis fenil sinamat dan 4-fenilkroman-2-on telah dilakukan dari metil sinamat dengan menggunakan katalis asam. Senyawa metil sinamat terlebih dahulu dikonversi menjadi asam sinamat melalui reaksi hidrolisis dengan basa. Selanjutnya asam sinamat diklorinasi menggunakan tionil klorida dan fenol dan menghasilkan fenil sinamat. Reaksi esterifikasi dengan fenol menggunakan katalis asam p-toluen sulfonat menghasilkan senyawa 4-fenilkroman-2-on. Dari peneitian ini diperoleh rendemen hasil sintesis asam sinamat, fenil sinamat dan 4-fenilkroman-2-on masing-masing adalah 83,6%, 14,71% dan 16,18%. Uji sitotoksisitas senyawa fenil sinamat dan 4-fenilkroman-2-on menggunakan metode brine shrimp lethality test (BSLT) diperoleh nilai LC50 masing-masing sebesar 223,87 dan 112,72 ppm. Hasil uji fenil sinamat dan 4-fenilkroman-2-on terhadap sel kanker serviks HeLa (ATCC CCL2) dengan metode MTT didapatkan persentase inhibisi masing-masing adalah di atas 50%.
Sintesis Analog 3-0-Benzylstentorin sebagai Prekursor untuk Kerangka Dasar Blepharismin ERNAWATI, TENI; USUKI, YOSHINOSUKE; IIO, HIDEO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 10 No 1 (2012): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Metode sintesis prekursor untuk kerangka dasar dan struktural analog blepharismin telah dijelaskan. Senyawa tersebut diidentifikasi sebagai obat yang potensial untuk pengobatan terapi antivirus dan photodynamic. Analog benzil stentorin sebagai kerangka dasar blepharismin secara efektif disintesis melalui reaksi antara salah satu gugus hidroksil senyawa biantrakuinon dengan p-metoksi benzyl klorida dengan adanya kalium karbonat sebagai basa. Dimerisasi senyawa turunan bromo antrakuinon menghasilkan produk dimmer sebesar 55% sedangkan untuk pembentukan senyawa analog 3-0-benzylstentorin diperoleh rendemen hasil 70%.