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Isolasi Dan Karakterisasi Terpenoid Serta Uji Antioksidan Dari Ekstrak Kulit Batang Shorea singkawang Efdi, Mai; Syafrizayanti, Syafrizayanti; Sari, Dian Kumala
CHEMPUBLISH JOURNAL Vol 1 No 2 (2016): Chempublish Journal
Publisher : Universitas Jambi

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Abstract

Isolasi dan karakterisasi terpenoid dari fraksi etil asetat ekstrak kulit batang Shorea singkawang telah dilakukan. Senyawa ini diisolasi dengan metode maserasi, kromatografi kolom, dan rekristalisasi serta dikarakterisasi dengan metode spektroskopi. Terpenoid tersebut berupa padatan putih dengan titik leleh 140-142°C dan berdasarkan data GC menunjukkan adanya 1 puncak. Berdasarkan data spektroskopi UV dan IR, senyawa hasil isolasi dalam pelarut metanol menunjukkan serapan maksimum pada panjang gelombang 201 nm dan memiliki gugus –OH, -CH2, -CH3, dan geminal dimetil namun belum diketahui posisi dari gugus-gugus tersebut. Hasil uji antioksidan dengan metode DPPH menunjukkan ekstrak etil asetat memiliki potensi sebagai antioksidan dengan EC50 sebesar 0,075%. Kata kunci : Shorea singkawang, terpenoid, uji antioksidan  
A Triterpenoid Compound From The Leaves of Lantana camaraLinn. Kartika, Kartika MZ; Suryati, Suryati; Efdi, Mai
Indonesian Journal of Fundamental and Applied Chemistry Vol 3, No 1 (2018): February 2018
Publisher : Indonesian Journal of Fundamental and Applied Chemistry

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Abstract

A triterpenoid compound has been isolated from the ethyl acetate fraction of Lantana camaraLinn leaves. The isolation was performed by chromatography of gravitation column with a gradient elution system using hexane, ethyl acetate and methanol. Purification by recrystallization produces a white solid (20 mg) with a melting point of 288-289 °C. The results of elucidation of UV, IR, 1H-NMR, 13C-NMR, HMBC, HSQC, DEPT and COSY spectral data, it is known that the isolated triterpenoid compound is 22β-angeloyloxy-9-hydroxy-3-oxo-olean-12- en-28-oicacid with molecular formula C35H52O6. Keywords—Lantana camaraLinn, Chromatography of gravitation column, Triterpenoid, C35H52O6
KAJIAN BIOAKTIFITAS DAN ANALISA KANDUNGAN SENYAWA METABOLIT SEKUNDER DARI TUMBUHAN Hornstedtia scyphifera var. fusiformis DENGAN HPLC (High Performance Liquid Chrimatography) Santoni, Adlis; Efdi, Mai; Suhada, Akmel
Jurnal Zarah Vol 5 No 2 (2017): Jurnal Zarah
Publisher : Program Studi Pendidikan Kimia, Fakultas Keguruan dan Ilmu Pendidikan Universitas Maritim Raja Ali Haji

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Abstract

Hornstedtia scyphifera var. fusiformis merupakan tumbuhan endemik dari famili Zingiberaceae yang tersebar di Sumatera. Tumbuhan ini  mengandung beberapa metabolit sekunder seperti fenolik, flavonoid, saponin, triterpenoid, steroid dan alkaloid. Pengujian Bioaktifitas meliputi uji antioksidan dan antibakteri. Aktivitas antioksidan ditentukan dengan metoda DPPH. Sedangkan aktivitas antibakteri diuji dengan metode difusi cakram terhadap bakteri Staphylococcus aureus (bakteri gram positif) dan Escherichia coli (bakteri gram negatif). Hasil analisa aktitifitas antioksidan dari ketiga ekstrak, diperoleh bahwa ekstrak metanol menunjukkan aktifitas antioksidan yang paling aktif dengan IC50 = 51,8943 mg/L dibandingkan dua ekstrak lainnya. Ketiga esktrak Hornstedtia scyphifera var. fusiformis memberikan aktivitas antibakteri, selanjutnya esktrak n-heksan dan etil asetat pada konsentrasi 500 ppm memiliki zona hambat 10 mm terhadap bakteri Escherichia coli  dan Staphylococcus aureus memiliki zona hambat 10,3 mm. Untuk mengetahui jumlah senyawa metabolit sekunder  yang terkandung dalam  tumbuhan ini dilakukan analisa dengan HPLC.
The potency of secondary metabolites from traditional medicine plants as anti cancer agent Efdi, Mai
Majalah Kedokteran Andalas Vol 37 (2014): Supplement 2 | Published in December 2014
Publisher : Faculty of Medicine Andalas University

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Abstract

The seconday metabolites from traditional medicine plants have played an important role incancer treatment. Exploration of natural active compounds from medicinal plants for cancertreatment has attracted substantial attention worldwide. Several secondary metabolites thatwere isolated fom the plants, such as vincristne, vinblastine, campthothecins, have been usedhave been used clinically for over 40 years. Numerous techniques have been developedto obtain compounds for drug discovery including isolation from plants, synthetic chemistry,screening bioassays. As part of efforts to discover phytochemicals as anticancer agent, ourgroup were isolated several compunds mainly from Annonaceae and Melliaceae family thatshowed active againts human leukemia HL-60 cells.
Isolation of Flavonoid Compound and Antioxidant Activity of Salix tetrasperma Roxb. Leaves Januarti, Ria; Santoni, Adlis; Efdi, Mai
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 2 (2019): June 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Salix Tetrasperma Roxb. is a plant that found in Indonesia were used as traditional medicine such as diabetes and wound healing. In this study, a flavonoid compound of the ethyl acetate extract of Salix tetrasperma Roxb. leaves was isolated by chromatography technique and the antioxidant activity was determined by DPPH assay. The isolation led to obtain 5,7-dihydroxy-3'-methoxyflavone based on NMR spectra. The ethyl acetate extract exhibited the highest antioxidant activity with the IC50 is 65.89 µg/mL. This study shows that the Salix tetrasperma Roxb. has good potential as source of antioxidant agent.
Antibacterial Activity and Structure Elucidation of Salicin from Stem Bark of Salix tetrasperma ROXB. Ramadhani, Sari; Santoni, Adlis; Efdi, Mai
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 2 (2019): June 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Salix tetrasperma Roxb. (Family Salicacaeae) is a plant that used as traditional medicine for anti-inflammatory, analgesic, reduces fever, and itching medicine. In this study was carried out extraction, isolation, structure elucidation of salicin from Salix tetrasperma Roxb. stem bark and it’s antibacterial activity. The extraction method was used the maceration method by n-hexane, ethyl acetate, and methanol solvents. Isolation of compound from ethyl acetate extract of Salix tetrasperma Roxb. stem bark using chromatography methods and obtained white solid (15 mg). The structure was elucidated using spectroscopic analysis, including Ultraviolet (UV), Infrared (IR), Nuclear Magnetic Resonance (NMR) and comparative literature, identified as salicin compound with molecule formula C13H18O7. Antibacterial activity was evaluated against Escherichia coli and Staphylococcus aureus bacteria using disk diffusion method. This compound has a great an antibacterial activity against Staphylococcus aureus bacteria with clear zone diameter of 10.2 ± 0.3 mm. This shows that the Salix tetrasperma Roxb. stem bark has great potential as a source of antibacterial compound
KAJIAN BIOAKTIFITAS DAN ANALISA KANDUNGAN SENYAWA METABOLIT SEKUNDER DARI TUMBUHAN Hornstedtia scyphifera var. fusiformis DENGAN HPLC (High Performance Liquid Chrimatography) Santoni, Adlis; Efdi, Mai; Suhada, Akmel
Jurnal Zarah Vol 5 No 2 (2017): Jurnal Zarah
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Maritim Raja Ali Haji

Show Abstract | Original Source | Check in Google Scholar | Full PDF (418.33 KB)

Abstract

Hornstedtia scyphifera var. fusiformis merupakan tumbuhan endemik dari famili Zingiberaceae yang tersebar di Sumatera. Tumbuhan ini  mengandung beberapa metabolit sekunder seperti fenolik, flavonoid, saponin, triterpenoid, steroid dan alkaloid. Pengujian Bioaktifitas meliputi uji antioksidan dan antibakteri. Aktivitas antioksidan ditentukan dengan metoda DPPH. Sedangkan aktivitas antibakteri diuji dengan metode difusi cakram terhadap bakteri Staphylococcus aureus (bakteri gram positif) dan Escherichia coli (bakteri gram negatif). Hasil analisa aktitifitas antioksidan dari ketiga ekstrak, diperoleh bahwa ekstrak metanol menunjukkan aktifitas antioksidan yang paling aktif dengan IC50 = 51,8943 mg/L dibandingkan dua ekstrak lainnya. Ketiga esktrak Hornstedtia scyphifera var. fusiformis memberikan aktivitas antibakteri, selanjutnya esktrak n-heksan dan etil asetat pada konsentrasi 500 ppm memiliki zona hambat 10 mm terhadap bakteri Escherichia coli  dan Staphylococcus aureus memiliki zona hambat 10,3 mm. Untuk mengetahui jumlah senyawa metabolit sekunder  yang terkandung dalam  tumbuhan ini dilakukan analisa dengan HPLC.