Found 6 Documents

Promoting Dolichoderus thoracicus as an Agent to Disperse Trichoderma sp., a Fungus that Controls the Black Pod Disease, Central Sulawesi, Indonesia Anggraeni, Tjandra; Umrah, U.; Esyanti, Rizkita R.; Aryantha, I Nyoman P.
Journal of Mathematical and Fundamental Sciences Vol 46, No 1 (2014)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.186 KB) | DOI: 10.5614/


In this study, we propose to use Dolichoderus thoracicus to act as a double agent; not only as an agent to control cocoa plant pests, but also as an agent for distributing Trichoderma sp., a fungus that suppresses the development of the black pod disease caused by Phytophthora palmivora. In the experiments, D. thoracicus was more attracted to honey-soil media (M5) and coconut waste pulp-soil media (M6). However, 10% sucrose-potato-soil media (M3) was the best media for growing Trichoderma sp. Morphological study showed that spores attached to many parts of D. thoracicus. The efficiency of D. thoracicus in distributing the spores compared to the conventional method of using a sprayer was measured. The results showed that the growth of P. palmivora was suppressed by 83.33%, which is not significantly different from when Trichoderma was dispersed through spraying (87% suppressed). It was concluded that D. thoracicus can act as a double agent and can be used to disperse Trichoderma sp.
Perilaku dan Penentuan Oviposisi dari Parasitoid Telur Hadronotus leptocorisae (Hymenoptera) pada Telur Hama Bulir Padi Leptocorisa acuta (Hemiptera) Anggraeni, Tjandra; Jamili, Aisah; Umrah, Umrah
Biocelebes Vol 4, No 2 (2010)
Publisher : Biocelebes

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.757 KB)


The research of behavior and host determination for the oviposition of egg parasitoid of Hadronotus leptocorisae on egg of the rice seed pests (Leptocorisa acuta) was carried out. H. leptocorisae collected from rice field were inserted into the tube containing of eggs of L. acuta which were divided into three group based on their ages; young eggs (0-1 days); mature eggs (2-3 days) and old eggs (4-5 days). The results showed that female H. leptocorisae prefer to oviposition on young eggs. Descriptive observation indicated that before eggs were laid, female H. leptocorisae inspect L. acuta eggs by rubbing of L. acuta eggs with its antenna and ovipositor. Furthermore, oviposition of H. leptocorisae eggs was begun by holing of L. acuta eggs and was followed by putting its eggs by its ovipositor. That behavior is its strategies to survive. The results of this study can contribute to answer pest L. acuta problems which are often found in rice fields. By the appropriate monitoring of L. acuta eggs in rice field, the disposal time of H. leptocorisae could be done effectively and efficiently.Key words: Hadronous leptocorisae; Leptocorisa acuta; egg parasitoid.
Infeksi Subletal Bacillus thuringiensis pada Helicoverpa armigera: Mungkinkah Menyebabkan Resurgensi? Anggraeni, Tjandra; Putra, Ramadhani Eka; Ahmad, Intan
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 1 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.10016


Research on the effect of B. thuringiensis sublethal infection to the chance of H. armigera to resurgence has been conducted. Third instar insects were used and the concentration applied were 0 ppm, 150 ppm, 300 ppm, 450 ppm and 600 ppm. The result showed that along with the increase of B. thuringiensis infection, the number and the viability of the eggs production declined. In addition, the weight difference between the 3rd and the 4th instar became higher however between the 4th and the 5th became lower, moreover, the weight of female imago decreased, the life longevity of male and female imago were shorter and longer, respectively, along with the increase of B. thuringiensis infection. The possibility for resurgence will be discussed.Key words: B. thuringiensis, H. armigera, sublethal, resurgence
Tanggapan Helicoverpa armigera terhadap Infeksi Subletal Nuclear Polyhedrosis Virus dan Dampaknya terhadap Laju Reproduksi Pawana, Gita; Anggraeni, Tjandra; H.D., Anna Laksanawati
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12387


The study on responses of Helicoverpa armigera Hbn. to sublethal HaNPV (H. armigera nuclear polyhedrosis virus) infection was carried out in the laboratory. The reproduction rate was scrutinized as their impact of HaNPVs exposures. Fifth instar of H. armigera larval was orally infected on sublethal serial dilution of HaNPV (1,800; 180; 18; 1.8; 0.18; 0.018 ppm from stock concentration of 1.1 X 10^9 PIB per ml suspension). A significantly female pupal weight forms the response of H. armigera to sublethal HaNPV infection on the concentration tested. However larval period, male pupal weight, pupal period, oviposition period, life span of imago, fecundity, fertility and survival did not significantly form the response of H. armigera to sublethal HaNPV infection. All treatment showed lower reproduction rate compared to the untreated, especially reproduction rate based on either debilitating effect and mortality or just based on debilitating effect.
Gonadal Maturity Induction using Karamunting (Melastoma malabatrhicum) Ethanol Extract on White Shrimp Female (Litopenaeus vannamei) Ridwan, Ahmad; Awaludin, Awaludin; Anggraeni, Tjandra
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Common problem that happened in white shrimp culturing is insufficiency of mature female to provide the seed simultaneously. There are several methods to accelerate gonadal maturity infemale white shrimp: eye ablation, environmental manipulation, and providing high cholesterol fresh feed. Till now, high cholesterol feed is not very common method. Karamunting or Malabar Melastome (Melasthoma malabatrhicum) is known to have a high cholesterol content, more specifically, lanosterol based on GCMS test. Lanosterol is cholesterol that contained in plants, which assumed as precursor hormone for gonad maturity process in female of white shrimp. The aim of this experiment is to determine whether Karamunting ethanol extract can stimulate and accelerate gonad development in female of white shrimp. This experiment consists of several steps: (1) Karamunting (Melastoma malabathricum) extraction, (2) GCMS test for Karamunting, (3) extract injection to female of white shrimp (Litopenaues vannamel) for 15 days in 3 days interval with dosage variable 0 (control), ), 10 mg/kg BW (P1), 7,5 mg/kg BW (P2), 5 mg/kg BW (P3), 2 mg/kg BW (P4) dan 1 mg/kg BW (P5), (4) The measuring parameters are Karamunting content, morphological gonad development observation and hepatopancreas somatic index (HSI) measurement, (5) Data analysis. Data shows that karamunting ethanol extract with P1 and P2 dosage can result in morphological gonad development response (GML II), meanwhile with P3, P4 and P5 dosage there is no changes observed. All variables didn’t affect the level of HSI (P>0.05). Based on the result, it can be concluded that Karamunting ethanol extract indicates an acceleration of gonad maturity process in white shrimp female.Keywords : Gonad maturity; Hepatopancreas Somatic Index ; L.  vannamei; M. malabatrhicum
Uji Hemaglutinasi Assay pada Hemolimf Spodoptera exigua yang diberi Perlakuan Botani Pestisida Suryani, A.Irma; Anggraeni, Tjandra
bionature Vol 20, No 1 (2019): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v20i1.9758


Abstract. The larval stage of the armyworm (Spodoptera exigua), has a high and polyphagous feeding activity so that these insects have the potential to be pests in agriculture. The use of botanical pesticides in this case Mirabilis jalapa, is expected to control agricultural pests so that research on the immune system of S. exigua larvae is carried out by observing lectins that play a role in analyzing foreign molecules that enter the insect's body. The results were obtained, the higher the M. jalapa concentration, at the concentration of M. jalapa 0.4% and 0.8%, the lower the hemolymf agglutination titers. This is thought to decrease the binding capacity of lectins to foreign molecules. So that M. jalapa has the potential to be used as an environmentally friendly botanical pesticide.Keywords: agricultural pests, botanical pesticides, lectin agglutination.