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Variabilitas Genetik dan Fenotipik Karakter Pertumbuhan dan Komponen Pertumbuhan Benih Hasil Perbanyakan Vegetatif Klon-klon Teh yang Diperoleh melalui Persilangan Buatan Khomaeni, Heri Syahrian; Rahadi, Vitria Puspitasari; Ruhaendi, Endi; Santoso, Budi
Jurnal Agro Vol 2, No 1 (2015)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.345 KB) | DOI: 10.15575/158

Abstract

Perbanyakan tanaman teh dengan menggunakan setek satu daun saat ini merupakan cara yang umum untuk memenuhi kebutuhan bahan tanaman dalam jumlah yang banyak dengan waktu yang singkat. Salah satu syarat dalam perakitan klon teh unggul baru adalah kemudahan klon tersebut untuk diperbanyak secara vegetatif. Hal ini dikarenakan klon yang mudah diperbanyak secara vegetatif lebih disukai oleh para pekebun. Salah satu pengujian yang harus dilakukan dalam proses pemuliaan tanaman teh adalah uji perbanyakan vegetatif pada kandidat klon unggul baru. Penelitian ini bertujuan untuk mengetahui variabilitas kemampuan pertumbuhan dan beberapa komponen pertumbuhan benih setek dalam uji perbanyakan vegetatif. Penelitian dilaksanakan di Persemaian Pemuliaan Kebun Percobaan Pusat Penelitian Teh dan Kina Gambung. Percobaan menggunakan Rancangan Acak Kelompok (RAK) dengan 19 klon sebagai perlakuan yang diulang tiga kali, setiap perlakuan ditanam 52 benih setek. Hasil penelitian menunjukkan bahwa hampir seluruh variabilitas genetik pada karakter yang diamati tergolong dalam kategori luas, kecuali untuk karakter panjang akar, dan berat kering akar. Variabilitas fenotipik menunjukkan bahwa hampir seluruh karakter yang diamati tergolong dalam kategori luas, kecuali berat kering akar. Currently, propagation of tea plant using one leaf is a common way to fulfil the need of plant material in large quantity also short in time. One of requirements in assembling new superior tea clone is the easy of clone to be propagated vegetatively. Clone which is easy to propagate in vegetative way is apt more by the gardeners. One required test on tea plant breeding process is vegetative propagation test of new superior clone candidates. This research aimed to determine variability of growth ability and several growth components of cutting seed in vegetative propagation. The research was carried out in Breeding Nursery, Experimental Field of Tea and Cinchona Research Institute Gambung. The trial was set in Randomized Block Design (RBD) with 19 clones as the treatment which was repeated three times, each treatment consisted of 52 cutting seeds. The result showed that almost all genetic variability of characters observed were included to wide category, except for root length and root dry weight characters. Phenotypic variability showed that nearly all characters observed were comprised to wide category, except root dry weight.
Correlations between morphological traits and catechin content on tea (Camellia sinensis (L.) O. Kuntze) Syahrian, Heri; Carsono, Nono; Rostini, Neni; Rahadi, Vitria Puspitasari; Sriyadi, Bambang
Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.981 KB) | DOI: 10.22302/pptk.jur.jptk.v18i1.64

Abstract

Catechin content in tea plant is a major character in tea breeding program for a healthy beverage. A tea breeding program for high catechin content maybe will performed by indirect selecting a trait or traits that signi­ficantly correlated with high catechin content. This research was performed to identify mor­phological traits which maybe significantly correlated with high catechin content in tea plant. A randomized complete block design with three replications was used in this experiment using 10 selected clones. The experiment was performed in Gambung Experimental Station ot the Research Institute for Tea and Cinchona, Gambung, West Java. Catechin content, tri­choma density of young leaves, weight of p+2, size of mother leaves, chlorophyll content of mother leaf, stomatal density of mother leaf, mother leaf thickness, and mother leaf angle, were measured. The results indicated that stomatal density and mother leaf angle were significantly correlated. Based on path analysis indirect selection for high catechin content could be performed by selecting stomatal density considering to trichoma density, mother leaf angle and chlorophyll content. In addition, indirect selection for high catechin content could be performed by selecting other leaf angle independently without considering other morphological traits.
The growth test of tea clones (Camellia sinensis) from cross pollination first generation clones Rahadi, Vitria Puspitasari; Syahrian, Heri; Sriyadi, Bambang
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.399 KB) | DOI: 10.22302/pptk.jur.jptk.v19i2.118

Abstract

Efforts to improve the productivity of tea needed new high yielding clones were obtained from the cross and easily propagated vegetatively. Preliminary testing of the clones  needs to be done to determine the growth of cuttings and rooting properties of tea clones from hybridization which are selected by potential production. This study used a Randomized Complate Block Design with three replications. The treatments consist of 14 clones from crosses and GMB 7 as clone comparison. The data is analyzed by using ANOVA and Scott Knott test with the level of 5%. The observations are intended to look at the growth of roots and shoots by dismantling seedling in polybag predetermined randomly at each observation. The results showed that the clones tested had good growth with a low mortality rate, and easily propagated vegetatively. The S / R ratio also showed shoot growth more faster than the growth of root. This character is advantageous because the most appropriate selection criteria for planting in the field.
Kadar pati akar dan sitokinin endogen pada tanaman teh menghasilkan sebagai dasar penentuan pemangkasan dan aplikasi zat pengatur tumbuh Anjarsari, Intan Ratna Dewi; Hamdani, Jajang Sauman; Victor Zar, Cucu Suherman; Nurmala, Tati; Sahrian, Heri; Rahadi, Vitria Puspitasari
Kultivasi Vol 17, No 2 (2018)
Publisher : Fakultas Pertanian UNPAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.569 KB) | DOI: 10.24198/kltv.v17i2.16786

Abstract

ABSTRAK Pemangkasan pada tanaman teh dilakukan salah satunya untuk menginisiasi tumbuhnya banyak tunas sebagai bakal pembentukan pucuk peko. Pemangkasan mengubah luas daun, kapasitas fotosintesis perdu, mempengaruhi keseimbangan metabolisme antara organ di atas dan di bawah tanah dengan  mengurangi  jumlah tumbuh tunas yang berfungsi sebagai sumber dan pengguna untuk nutrisi dan hormon. Sampai saat ini pertumbuhan tunas sebagai bakal daun setelah pemangkasan terjadi secara alami tanpa penambahan zat pengatur tumbuh (ZPT). Pada dasarnya rekayasa fisiologis dengan menggunakan ZPT sitokinin dapat menjadi pilihan untuk lebih memacu pertumbuhan cabang lateral dan tunas serta memecahkan dormansi pucuk. Tujuan penelitian pendahuluan ini  adalah untuk mengetahui   kadar pati akar, kadar sitokinin endogen, serta status hara tanah  guna menentukan waktu pemangkasan yang tepat dan dasar untuk dilakukan aplikasi zat pengatur tumbuh setelah dipangkas. Penelitian selanjutnya adalah penggunaan sitokinin BAP pada berbgai dosis pada tanaman teh yang sudah dipangkas.  Penelitian pendahuluan dilakukan pada bulan Agustus  hingga Oktober 2017 di kebun percobaan Pusat Penelitian Teh dan Kina (PPTK) Gambung  pada ketinggian 1250 m di atas permukaan laut (dpl). Metode pengambilan sampel daun, akar, dan tanah di lapangan dilakukan secara komposit untuk setiap ulangan selanjutnya dilakukan analisis pati akar, sitokinin endogen serta hara tanah. Hasil uji kualitatif pati akar menggunakan iodium mengindikasikan bahwa tanaman teh siap untuk dipangkas terlihat dari sampel akar yang ditetesi iodium menunjukkan warna hitam. Hasil analisis laboratorium menunjukkan bahwa kadar pai akar berada pada kisaran 6.99 % hingga 9,16% dan sitokinin endogen ada pad akisaran 0,0016% hingga0,0019%.  Penentuan kadar pati akar, kondisi lingkungan serta status hara sebelum pemangkas diperlukan agar meminimalisasi tingkat kematian perdu teh serta analisis sitokinin endogen diperlukan untuk lebih  mengoptimalkan dosis sitokinin yang akan diberikanKata Kunci : pemangkasan, sitokinin endogen, kadar pati akar. ABSTRACT  Pruning on tea plants is  perfomed initiating growth of shoots to be pecco stadia. Pruning changes the leaf area, the capacity of photosynthetic tea bush, affecting the metabolic balance between upper and underground organs by reducing the growing number of buds that function as sources and sinks for nutrients and hormones. Until now the growth of shoots as leaf will after pruning occurs naturally without the addition of plant growth regulating substances (PGR). Essentially physiological engineering using  cytokinins can be an option to increase the growth of lateral branches and buds as well as break the shoot dormancy. The preliminary study was conducted from August to October 2017 at experimental field of Gambung Tea and Quinine Research Center (PPTK) at an altitude of 1250 m above sea level (asl). Preliminary method used in the form of analysis of root starch, endogenous cytokinin and soil nutrients to  determined the proper pruning time and the basis for the application of  plant growth regulator substances after pruning. The results of a qualitative test of root content using iodine indicated that the tea plant was ready to be pruned visible from the root samples that iodized spots showed black. The result of  laboratory test  showed that root starch content was in the range of 6.99 to 9.16. and cytokinin endogen  preliminary analysis showed that the levels are in the range of 0.0016 up to 0.0019. Determination of root starch, environmental conditions and nutrient status before pruning is necessary in order to minimize mortality rate of tea bush as well as analysis of endogenous cytokinin is needed to further optimize the dose of cytokinin to be given. Keywords : cytokinins, pruning,  root starch content
Diseases Classification for Tea Plant Using Concatenated Convolution Neural Network Krisnandi, Dikdik; Pardede, Hilman F.; Yuwana, R. Sandra; Zilvan, Vicky; Heryana, Ana; Fauziah, Fani; Rahadi, Vitria Puspitasari
CommIT (Communication and Information Technology) Journal Vol 13, No 2 (2019): CommIT Vol. 13 No. 2 Tahun 2019 (In Press)
Publisher : Bina Nusantara University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21512/commit.v13i2.5886

Abstract

Plant diseases can cause a significant decrease in tea crop production. Early disease detection can help to minimize the loss. For tea plants, experts can identify the diseases by visual inspection on the leaves. However, providing experts to deal with disease identification may be very costly. The machine learning technology can be implemented to provide automatic plant disease detection. Currently, deep learning is state-of-the-art for object identification in computer vision. In this study, the researchers propose the Convolutional Neural Network (CNN) for tea disease detections. The researchers focus on the implementation of concatenated CNN, namely GoogleNet, Xception, and Inception-ResNet-v2, for this task. About 4727 images of tea leaves are collected, comprising of three types of diseases that commonly occur in Indonesia and a healthy class. The experimental results confirm the effectiveness of concatenated CNN for tea disease detections. The accuracy of 89.64% is achieved.
Kadar pati akar dan sitokinin endogen pada tanaman teh menghasilkan sebagai dasar penentuan pemangkasan dan aplikasi zat pengatur tumbuh Anjarsari, Intan Ratna Dewi; Hamdani, Jajang Sauman; Victor Zar, Cucu Suherman; Nurmala, Tati; Sahrian, Heri; Rahadi, Vitria Puspitasari
Kultivasi Vol 17, No 2 (2018)
Publisher : Fakultas Pertanian UNPAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kltv.v17i2.16786

Abstract

ABSTRAK Pemangkasan pada tanaman teh dilakukan salah satunya untuk menginisiasi tumbuhnya banyak tunas sebagai bakal pembentukan pucuk peko. Pemangkasan mengubah luas daun, kapasitas fotosintesis perdu, mempengaruhi keseimbangan metabolisme antara organ di atas dan di bawah tanah dengan  mengurangi  jumlah tumbuh tunas yang berfungsi sebagai sumber dan pengguna untuk nutrisi dan hormon. Sampai saat ini pertumbuhan tunas sebagai bakal daun setelah pemangkasan terjadi secara alami tanpa penambahan zat pengatur tumbuh (ZPT). Pada dasarnya rekayasa fisiologis dengan menggunakan ZPT sitokinin dapat menjadi pilihan untuk lebih memacu pertumbuhan cabang lateral dan tunas serta memecahkan dormansi pucuk. Tujuan penelitian pendahuluan ini  adalah untuk mengetahui   kadar pati akar, kadar sitokinin endogen, serta status hara tanah  guna menentukan waktu pemangkasan yang tepat dan dasar untuk dilakukan aplikasi zat pengatur tumbuh setelah dipangkas. Penelitian selanjutnya adalah penggunaan sitokinin BAP pada berbgai dosis pada tanaman teh yang sudah dipangkas.  Penelitian pendahuluan dilakukan pada bulan Agustus  hingga Oktober 2017 di kebun percobaan Pusat Penelitian Teh dan Kina (PPTK) Gambung  pada ketinggian 1250 m di atas permukaan laut (dpl). Metode pengambilan sampel daun, akar, dan tanah di lapangan dilakukan secara komposit untuk setiap ulangan selanjutnya dilakukan analisis pati akar, sitokinin endogen serta hara tanah. Hasil uji kualitatif pati akar menggunakan iodium mengindikasikan bahwa tanaman teh siap untuk dipangkas terlihat dari sampel akar yang ditetesi iodium menunjukkan warna hitam. Hasil analisis laboratorium menunjukkan bahwa kadar pai akar berada pada kisaran 6.99 % hingga 9,16% dan sitokinin endogen ada pad akisaran 0,0016% hingga0,0019%.  Penentuan kadar pati akar, kondisi lingkungan serta status hara sebelum pemangkas diperlukan agar meminimalisasi tingkat kematian perdu teh serta analisis sitokinin endogen diperlukan untuk lebih  mengoptimalkan dosis sitokinin yang akan diberikanKata Kunci : pemangkasan, sitokinin endogen, kadar pati akar. ABSTRACT  Pruning on tea plants is  perfomed initiating growth of shoots to be pecco stadia. Pruning changes the leaf area, the capacity of photosynthetic tea bush, affecting the metabolic balance between upper and underground organs by reducing the growing number of buds that function as sources and sinks for nutrients and hormones. Until now the growth of shoots as leaf will after pruning occurs naturally without the addition of plant growth regulating substances (PGR). Essentially physiological engineering using  cytokinins can be an option to increase the growth of lateral branches and buds as well as break the shoot dormancy. The preliminary study was conducted from August to October 2017 at experimental field of Gambung Tea and Quinine Research Center (PPTK) at an altitude of 1250 m above sea level (asl). Preliminary method used in the form of analysis of root starch, endogenous cytokinin and soil nutrients to  determined the proper pruning time and the basis for the application of  plant growth regulator substances after pruning. The results of a qualitative test of root content using iodine indicated that the tea plant was ready to be pruned visible from the root samples that iodized spots showed black. The result of  laboratory test  showed that root starch content was in the range of 6.99 to 9.16. and cytokinin endogen  preliminary analysis showed that the levels are in the range of 0.0016 up to 0.0019. Determination of root starch, environmental conditions and nutrient status before pruning is necessary in order to minimize mortality rate of tea bush as well as analysis of endogenous cytokinin is needed to further optimize the dose of cytokinin to be given. Keywords : cytokinins, pruning,  root starch content