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The Effect of Chitosan on Lipid Profile of Sprague Dawley Rat Blood Serum Martati, Erryana; Lestari, Lily Arsanti; Lestari, Lily Arsanti
Jurnal Teknologi Pertanian Vol 9, No 3 (2008)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Chitosan shares characteristic with dietary fiber which can interact with lipids and/or  cholesterol in the intestinal lumen, consequently, reduce their absorption.  The effect of chitosan concentration on the serum cholesterol levels (total cholesterol, HDL, LDL cholesterol and trigliceride) and digesta profile of Sprague Dawley rats were investigated. A two month old Sprague Dawley rats consisted of six groups with seven animals of each group were adapted for one week by giving standard diet (AIN-93). Then they were treated with hypercholesterol diet containing approximately 200 mg/kg cholesterol for one week.  At the end of this week, cholesterol level was measured. The parameter for hypercholesterolemic rats was the raising level of serum cholesterol as much as 130 mg/dl. The first three groups of rats were treated with standard diet (AIN-93) with 0%, 2.5%, and 5%  of chitosan.  The second three groups were treated with hypercholesterol diet with the same treatments as the first one. Blood was drawn from eyes (reorbital plexus) every week for analyzing cholesterol levels (total cholesterol, HDL, LDL cholesterol and triglicerides). At the end of the treatment, seven rats were killed after one day fasting then liver and caecum were collected for analyzing.Research showed that chitosan of 2.5% and 5% in the diet did not alter body and liver weight of the group with standard diet (AIN-93) treatment. Conversely, body wieght decreased and liver weight increased in the hypercholesterol diet treatments. Chitosan also increased digesta weight and cholesterol level either in standard or hypercholesterol diet treatments. Serum profiles showed that chitosan treatment of 2.5% or 5% decreased total cholesterol, LDL cholesterol and triglyceride but increased HDL cholesterol.Keyword: lipid profile, blood serum, chitosan, hypercholesterol
The Effect of Chitosan on Lipid Profile of Sprague Dawley Rat Blood Serum Martati, Erryana; Lestari, Lily Arsanti; Lestari, Lily Arsanti
Jurnal Teknologi Pertanian Vol 9, No 3 (2008)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Chitosan shares characteristic with dietary fiber which can interact with lipids and/or  cholesterol in the intestinal lumen, consequently, reduce their absorption.  The effect of chitosan concentration on the serum cholesterol levels (total cholesterol, HDL, LDL cholesterol and trigliceride) and digesta profile of Sprague Dawley rats were investigated. A two month old Sprague Dawley rats consisted of six groups with seven animals of each group were adapted for one week by giving standard diet (AIN-93). Then they were treated with hypercholesterol diet containing approximately 200 mg/kg cholesterol for one week.  At the end of this week, cholesterol level was measured. The parameter for hypercholesterolemic rats was the raising level of serum cholesterol as much as 130 mg/dl. The first three groups of rats were treated with standard diet (AIN-93) with 0%, 2.5%, and 5%  of chitosan.  The second three groups were treated with hypercholesterol diet with the same treatments as the first one. Blood was drawn from eyes (reorbital plexus) every week for analyzing cholesterol levels (total cholesterol, HDL, LDL cholesterol and triglicerides). At the end of the treatment, seven rats were killed after one day fasting then liver and caecum were collected for analyzing.Research showed that chitosan of 2.5% and 5% in the diet did not alter body and liver weight of the group with standard diet (AIN-93) treatment. Conversely, body wieght decreased and liver weight increased in the hypercholesterol diet treatments. Chitosan also increased digesta weight and cholesterol level either in standard or hypercholesterol diet treatments. Serum profiles showed that chitosan treatment of 2.5% or 5% decreased total cholesterol, LDL cholesterol and triglyceride but increased HDL cholesterol.Keyword: lipid profile, blood serum, chitosan, hypercholesterol
Pola konsumsi pangan dan tingkat ketahanan pangan rumah tangga di Kabupaten Kampar Provinsi Riau Saputri, Rahmadya; Lestari, Lily Arsanti; Susilo, Joko
Jurnal Gizi Klinik Indonesia Vol 12, No 3 (2016): Januari
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.823 KB) | DOI: 10.22146/ijcn.23110

Abstract

Background: Food consumption is a major factor to fulfill the nutritional needs of a person. Food consumption is influenced by several factors, such as the level of income, food availability, public awareness toward nutrition, and socio-cultural factors. Riau Province is one of the provinces that the food consumption is still relatively low and Kampar Regency occupies a very high position in the food insecurity index and hunger vulnerability index. There is 49.21% of the population that could potentially be food shortages. This is supported by the occurrence of nutritional problems in communities associated with the availability of food, low purchasing power and the inability to gain access to nutritious food.Objective: To identify the patterns of food consumption and family food security in Kampar regency in Riau province.Method: The study was an observational research with cross sectional design. Samples are a family in Kampar regency as many as 105 families selected by using cluster sampling conducted in August-September 2015. Statistical analysis was performed by chi-square test and logistic regression.Results: The statistical analysis shows that the majority of respondents had food consumption patterns with the category of unfulfilled as 64.77% and for the category of food security as 60.96% was in the category of food insecure. The results of chi-square statistical test showed that there was a significant correlation between food consumption patterns to family food security in Kampar regency Riau Province (p0,000). The results of the bivariate analysis it is known that there was a significant correlation between the variables of the number of family member, food access, food expenses, energy consumption and protein consumption to the level of family food security in Kampar regency of Riau province indicated by a value of p <0.05.Conclusion: There is a significant correlation between the patterns of food consumption toward family food security in Kampar regency Riau province. There is a significant correlation between the external variables and family food security level in Kampar regency Riau Province.
Peningkatan aktivitas fagositosis dan produksi nitrit oksida pada makrofag peritoneum tikus Sprague Dawley yang diberi Lactobacillus plantarum Mut7 dan ekstrak serat ubi jalar Lestari, Lily Arsanti; Soesatyo, Marsetyawan Heparis Nur Ekandaru; Iravati, Susi; Harmayani, Eni
Jurnal Gizi Klinik Indonesia Vol 9, No 2 (2012): Oktober
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.15381

Abstract

Background: Macrophages play an important role as part of the innate immune response in the gut and they represent one of the first lines of nonspecific defense against bacterial invasion. Previous studies indicated that probiotics and prebiotics may act as an immunomodulator agents. Nevertheless, research on the immunomodulatory effect of local materials has never been performed.Objective: To study the effects of Lactobacillus plantarum Mut7 and sweet potato fiber on the activity and Nitric Oxide (NO) production of peritoneal macrophages of Sprague Dawley rats.Method: Ninety six Sprague Dawley rats aged 8 weeks were divided into two groups; A (not infected with Salmonella typhimurium) and B (infected with S. typhimurium). Each group was divided into 4 subgroups and assigned to standard AIN-93M diet (KON), 109 CFU/ml of L. plantarum Mut7 (PRO), modified AIN-93M diet with sweet potato fiber (PRE), and both component (SIN). After 3 weeks of treatment, 6 rats of each subgroup were sacrificed and the peritoneal macrophages were isolated and analysed for its activity and NO production. The rest of the rats continued the treatments for another 2 weeks. At the end of the experiment, they were sacrificed and the peritoneal macrophage were isolated and analysed for its activity and NO production.Results: Oral administration of  L. plantarum Mut7, sweet potato fiber, or both improve phagocytic activity of peritoneal macrophage which was indicated by an increase in the percentage of macrophages that phagocyte latex particles (p<0.05) and an increase in the number of latex particles engulfed by macrophages either after 3 or 5 weeks of treatment (p<0.05). Oral administration of L. plantarum Mut7, sweet potato fiber, or both were unable to increase the nitric oxide production after 3 weeks of treatment (p>0.05), but after 5 weeks of treatment the production of NO was significantly increased (p<0.05).Conclusion: L. plantarum Mut7, sweet potato fiber, or both increase the non specific immune response as they could improve the activity and NO production of peritoneal macrophages.
Swamedikasi diabetes mellitus dengan daun ceplikan (Ruellia tuberosa L.): kajian kemanfaatan pada profil lipid serum secara praklinik Ristanti, Ety Yuni; Lestariana, Wiryatun; Lestari, Lily Arsanti
Jurnal Gizi Klinik Indonesia Vol 5, No 3 (2009): Maret
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.17569

Abstract

Background: Self-medication by using ceplikan leaves (Ruellia tuberosa L.) has been practiced in society for many times in the after-boiled form. It had been proved that liquid extract of ceplikan leaves (Ruellia tuberosa L.) was able to reduce blood glucose level on diabetic rat. It is also well known among diabetes mellitus patient that it has following effect on increasing triglyceride and reducing HDL-cholesterol level.Objective: This research was aimed to give scientific evidence on the society habit on self-medication by using ceplikan leaves (Ruellia tuberosa L.) by examining its oral administration on serum lipid profile pre-clinically.Method: Two months old Rattus novergicus within average weight of 150-200 g were divided into experimental and control groups. The control group consisted of 6 normal rats; while the experimental group consisted of 24 alloxan induced-diabetic rats. The experimental groups were divided into four groups i.e. non-treatment group and oral administrations of Ruellia tuberosa L. liquid extract with different concentrations that were 1.6 mg, 3.2 mg, and 6.4 mg, respectively. The feeds were given ad libitum. The oral administrations of Ruellia tuberosa L. liquid extract were given everyday for 30 days. In the end of the treatment, all rats were fastened and the blood was taken from the eye and being observed for its serum lipid profile. The data were analyzed statistically by using ANOVA and Tukey’s HSD mean difference tests.Results: The results showed that oral administration of 3.2 mg Ruellia tuberosa L. liquid extract was significantly reduced triglyceride and LDL-cholesterol level (p < 0,05) and increased HDL-cholesterol level (p < 0,05).Conclusion: The 3.2 mg of Ruellia tuberosa L. liquid extract was able to reduce triglyceride and LDL-cholesterol level and increase HDL-cholesterol level. Moreover, ceplikan leaves (Ruellia tuberose L.) could be taken as traditional drugs in a non-formal way with control on dietary intake and regular physical exercise.
Pengaruh pemberian kecambah kacang hijau (Phaseolus radiatus (L.)) terhadap kadar malondealdehid (MDA) plasma dan jaringan hati tikus Sprague Dawley yang diberi pakan lemak tinggi Novidiyanto, Novidiyanto; Farmawati, Arta; Lestari, Lily Arsanti
Jurnal Gizi Klinik Indonesia Vol 13, No 2 (2016): Oktober
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.22923

Abstract

Background: High-fat diet increases the levels of fat, especially cholesterol and triglycerides that cause hyperlipidemia. Polyunsaturated fatty acids are very easily oxidized by free radicals reactive oxygens species (ROS) called lipid peroxidation, to produce compounds that malondialdehyde (MDA), is toxic and can damage plasma membrane and liver tissue. Mung bean sprouts are known to contain antioxidants which act to inhibit lipid peroxidation process.Objective: Determine the effect of mung bean sprouts to the level of plasma MDA and liver tissue of rats with high-fat diets.Method: This research is an experimental study with an only post-test design with the control group. Thirty-five male Sprague-Dawley rats were divided into 5 groups. Group K1 received fed standard; K2 received high-fat diet without mung bean sprouts. Group P1 received high-fat diet and mung bean sprouts dose of  0.5 mL/gBW. Group P2 received high-fat diet and mung bean sprouts dose of 1 mL/gBW. Group P3 received high-fat diet and vitamin E doses of 23 IU. All group were treated for 28 days. Analysis of MDA plasma and liver tissue performed after treatment.Results: Group K1 has plasma MDA levels of 1.17 ± 0.14 nmol/mL Mean MDA plasma level in the group K1 Group of K1 Mean MDA plasma level in the group K2 (39.7±0.19 nmol/mL) was higher than the group of K1 (1,17±0,14 nmol/mL), group P1 (2.94±0.09 nmol/mL), group P2 (1.73±0.08 nmol/mL) and group P3 (1.53±0.07 nmol/mL). Group K1 has liver tissue MDA Levels of 1,64±0,11 nmol/g. Mean MDA liver tissue level in the group of K2 (4.90±0.29 nmol/g) was higher than the group of P1 (3.68±0.45 nmol/g), group P2 (2.27±0.10 nmol/g), and group P3 (2.01±0.11 nmol/g).Conclusion: Level of MDA plasma and liver tissue of rat in group P1 and P2 was lower than a level of MDA plasma and liver tissue of rat in group K1, but higher than the level of MDA plasma and liver tissue of rat in group P3. There was a very strong correlation between the level of MDA plasma and liver tissue of rat was given the mung bean sprouts and high-fat diet.
Pelatihan hygiene sanitasi dan poster berpengaruh terhadap pengetahuan, perilaku penjamah makanan, dan kelaiakan hygiene sanitasi di instalasi gizi RSUP Sanglah Denpasar Rapiasih, Ni Wayan; Prawiningdyah, Yeni; Lestari, Lily Arsanti
Jurnal Gizi Klinik Indonesia Vol 7, No 2 (2010): November
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.17738

Abstract

Background: The problem of food hygiene sanitation in hospital is closely related to the incidence of nosocomial infection which is estimated to be high considering that the condition of hospitals and health in general is still relatively not very good. A way to transmit nosocomial infection is through food. Staff hygiene is a factor requiring attention in order that the product of nutrition installation is of quality and safe to consume.Objective: To identify the effect of training and posting of food hygiene sanitation to knowledge, healthy behavior of food handlers in efforts to improve food safety and sanitation hygiene appropriateness.Method: This was a time series  quasi experiment with one group pre test and post test design. Samples consisted of 44 people of senior level education in charge of food service to patient in class I, II, and III. Every subject was given training using lecture, discussion and demonstration method for one day. Poster was posted a month after training. Data obtained consisted of sex, age, marital status, duration of occupation, place of work, knowledge, behavior, food safety in food serving utensil for inpatients and sanitation hygiene appropriateness. Assessment was made before, one month and two months after training. Data analysis used paired Sample t-test and chi-square.Results: There was signifcant improvement in knowledge and healthy behavior before and  after training plus poster showed with p<0.001. There was signifcant difference in sex and behavior before training with p=0.045 and a month after training with p<0.001. There was signifcant association between place of work and behavior a month after training with p=0.021. There was signifcant difference in total plate count one month and two months after training with p=0.049. In addition, there was an increased quality of sanitation hygiene appropriateness before and  after training plus poster showed.Conclusion: There was an increasing of knowledge, behavior of food handlers, sanitation hygiene appropriateness, before and after training plus poster. There  was an  improvement in quality food safety one and two months after training.
Pola konsumsi pangan dan tingkat ketahanan pangan rumah tangga di Kabupaten Kampar Provinsi Riau Saputri, Rahmadya; Lestari, Lily Arsanti; Susilo, Joko
Jurnal Gizi Klinik Indonesia Vol 12, No 3 (2016): Januari
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.23110

Abstract

Background: Food consumption is a major factor to fulfill the nutritional needs of a person. Food consumption is influenced by several factors, such as the level of income, food availability, public awareness toward nutrition, and socio-cultural factors. Riau Province is one of the provinces that the food consumption is still relatively low and Kampar Regency occupies a very high position in the food insecurity index and hunger vulnerability index. There is 49.21% of the population that could potentially be food shortages. This is supported by the occurrence of nutritional problems in communities associated with the availability of food, low purchasing power and the inability to gain access to nutritious food.Objective: To identify the patterns of food consumption and family food security in Kampar regency in Riau province.Method: The study was an observational research with cross sectional design. Samples are a family in Kampar regency as many as 105 families selected by using cluster sampling conducted in August-September 2015. Statistical analysis was performed by chi-square test and logistic regression.Results: The statistical analysis shows that the majority of respondents had food consumption patterns with the category of unfulfilled as 64.77% and for the category of food security as 60.96% was in the category of food insecure. The results of chi-square statistical test showed that there was a significant correlation between food consumption patterns to family food security in Kampar regency Riau Province (p0,000). The results of the bivariate analysis it is known that there was a significant correlation between the variables of the number of family member, food access, food expenses, energy consumption and protein consumption to the level of family food security in Kampar regency of Riau province indicated by a value of p <0.05.Conclusion: There is a significant correlation between the patterns of food consumption toward family food security in Kampar regency Riau province. There is a significant correlation between the external variables and family food security level in Kampar regency Riau Province.
Pengaruh ekstrak teh hijau (Camellia sinensis) terhadap kadar malondialdehid (MDA) plasma dan kekuatan genggam tangan lanjut usia Nofiartika, Fera; Susetyowati, Susetyowati; Pramantara, I Dewa Putu; Lestari, Lily Arsanti
Jurnal Gizi Klinik Indonesia Vol 13, No 2 (2016): Oktober
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.22833

Abstract

Background: Prevalence of Indonesian elderly has now reached 7%, which is considerably high. Physical performance is an important health status indicator in elderly. Declining of physical performance such as hand grip strength in elderly is related to oxidative stress. Furthermore, oxidative stress plays an important role on the decreasing of skeletal muscle function. Plasma malondialdehyde (MDA) is a lipid peroxidation residue that is often used as an oxidative stress biomarker. Meanwhile, tea is the most widely drunk beverage after water. Green tea contains antioxidants that can reduce the damages caused by oxidative stress. Objective: To assess the effect of green tea extract (GTE) on plasma malondialdehyde level and hand grip strength of elderly.Method: This was a double-blind experimental with pre-post test and control group. Fifty-seven elderly people from Balai Pelayanan Social Tresna Wredha (BPSTW) Abiyoso and Budi Luhur who met inclusion and exclusion criteria were divided into 2 groups. During 30 days, the first group was given 2 capsules of GTE a day (±168 mg catechin and 95 mg Epigallocatechin gallate (EGCG) and the second group was given a placebo. MDA was measured by thiobarbituric acid  (TBA)method and hand grip strength was measured by handgrip hand grip dynamometer. Results: There was a significant decreasing MDA (p<0,05) in the GTE group, but not in the placebo group. There was also increasing handgrip strength in the GTE group but not in the placebo group. Conclusion: Green tea extract can prevent increasing MDA and declining physical handgrip strength in elderly.
Efek F100 dan formula tepung tempe terhadap kadar serum Fe dan hemoglobin pada anak gizi kurang Iva, Tsalissavrina; Prawirohartono, Endy Paryanto; Lestari, Lily Arsanti
Jurnal Gizi Klinik Indonesia Vol 9, No 1 (2012): Juli
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.15373

Abstract

Background: Protein energy deficiency is a major public health problem in Indonesia. WHO has recommended F100 made from skim milk for undernourished patient. Alternative formulas have been developed using other nutritious foods that are cheaper, easily accessible and can be used for children with lactose intolerance such as tempe flour.Objective: To investigate the effect of F100 and tempe flour formula supplementation on serum Fe and hemoglobin (Hb) levels of undernourished child.Method: This was an experimental study with randomized controlled clinical trial design and purposive sampling method. Subjects of the study were undernourished patients aged 1-10 years hospitalized at Dr. Saiful Anwar Hospital Malang. Sample consisted of 30 patients divided into 2 groups; group 1 supplemented with F100 and group 2 with tempe flour formula. Hb level was assessed by cyanmethemoglobin and serum Fe level by colorimetric method. Dietary intake data was collected by visual comstock and food recall. Data were analyzed by chi square, unpaired t-test and double linear regression.Results: Statistical analysis showed that there were no significant differences in Hb (p=0.139) and serum Fe levels (p=0.313) between both groups after treatment. Intake data indicated that there was a significant disparity in protein (p=0.019) and Fe intake (p=0.006) between the two groups, whereas energy, fat and carbohydrate intake showed no significant differences. Lastly, the association between energy and nutrient intake with serum Fe and Hb levels was not significant and the correlation was weak (r<1).Conclusion: There were no significant differences in hemoglobin and serum Fe levels between F100 group and tempe flour formula group, but there was a significant difference in protein and Fe intake.