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PENGARUH PENYUPLEMENAN Spirulina DALAM PAKAN TERHADAP HEMATOLOGIS IKAN NILEM (Osteochilus hasselti C.V.) EFFECT OF SUPPLEMENTED Spirulina IN DIET ON NILEM FISH (Osteochilus hasselti C.V.) HAEMATOLOGYS

Pembangunan Pedesaan Vol 6, No 2 (2006)
Publisher : Pembangunan Pedesaan

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Abstract

The aim of this research was to know the effect of supplemented Spirulina in diet on nilem fish haematologys and to obtain the best dose. Pre-treated fishes were acclimated to the laboratory environment for 10 days then allocated randomly into 4 treatment groups. Each group consisted of three aquaria containing 100 L of water equipped with recirculatory system, 12 fishes were allocated into each aquarium. Fish in group A served as control given pellet without Spirulina. Fishes in groups B, C, and D were given pellet supplemented with 2, 4, and 6 g.kg-1 Spirulina, respectively, given daily at 07.30 and 16.00 at 5% of body weight. The results showed that haematologys profile on the nilem fish was affected by Spirulina. This was indicated by increasing erythrocyte counts, total leucocyte, and haemoglobin level as well as haematocrite value. Supplement with 4 g.kg-1 Spirulina was the best dose.

Peran Hormon Kortisol dalam Osmoregulasi Ikan Sidat, Anguilla bicolor, pada Lingkungan Bersalinitas

Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

The osmoregulatory capacity of Anguilla bicolor in the sea water is influenced by hormonal activities.  Therefore, the aim of this study was to know the influence of cortisol on osmoregulation of the eel at the different levels of salinity medium. An experimental method with six treatments on randomized completely design was used in this study. The treatments were (1) fish acclimated at water salinity 15 ppt without hormone injection; (2) fish acclimated at water salinity of 30 ppt without hormone injection; (3) fish acclimated at water salinity of 15 ppt and injected with 4 µg cortisol/gr body weight; (4) fish acclimated at water salinity 15 ppt and injected with 8 µg cortisol/gr body weight; (5) fish aclimated at water salinity of 30 ppt and injected with 4 µg cortisol/g body weight; (6) fish acclimated at water salinity of 30 ppt and injected with 8 µg cortisol/g body weight. All of the treatments were replicated four times. Data were analyzed using One way ANOVA followed by Least Significant Difference. The results showed that the cortisol has significant effect (P<.05) on plasma osmolality only at the early of acclimation on medium 30 ppt after injection, especially at 6 and 12 hours after cortisol treatment, and there was no significant efffect of cortisol treatment (P>.05) if the acclimation increased. Osmoregulatory capacity on medium of 15 ppt and 30 ppt were influenced by cortisol treatment at 6 hours acclimation (P<.05), but only that aclimated in medium opf 5 ppt was influenced by cortisol treatment at 12 hours acclimation. Increased acclimation on medium of 15 ppt and 30 ppt was not influenced by cortisol treatment (P>.05). Water body content was not influenced by cortisol treatment (P>.05) at all medium acclimation. At six hour acclimation, treatment cortisol has significant difference (P<.05) on hematocrite only at medium 30 ppt, but cortisol treatment has significant difference (P<.05) at medium 15 ppt and 30 ppt in 12 hours acclimation. Increased acclimation has no significant difference (P>.05) on treatment cortisol. Cortisol injection has no significant difference (P>.05) on plasma glucose on all medium and only on 7 days aclimation, the plasma glucose has significant difference (P<.05) after injected by cortisol. It could be concluded that cortisol treatment has a role on eel osmoregulation at early acclimation. Haematocrite account was also influenced by cortisol injection, but only at the early acclimation. Water body content, blood glucose and total body energy were not influenced by cortisol.

Karakteristik Enzim Digesti, Protease dan Amilase, Ikan Gurami (Osphronemus gouramy Lac.) pada Fase Pertumbuhan

Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 2 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Suatu penelitian untuk mengetahui karakteristik enzim digesti, protease dan amilase pada ikan gurami, Osphronemus gouramy Lac., telah dilakukan dengan metode survey. Jumlah ikan yang digunakan untuk penelitian sebanyak 25 ekor yang dikelompokan menjadi tiga kelompok ukuran13,29, 35,86 dan 91,86 g/ekor. Hasil penelitian menunjukkan bahwa aktivitas protease digesti ikan gurami berbeda secara signifikan pada segmen usus dan pH buffer yang berbeda (P<.05). Aktivitas amilase digesti tidak berbeda secara signifikan diantara segmen usus yang diuji (P>.05), namun berbeda secara signifikan diantara ukuran ikan yang berbeda (P<.05), dan aktivitas amilase tertinggi dijumpai pada ikan dengan ukuran terkecil. Kesimpulan yang dapat diambil adalah bahwa aktivitas protease dan amilase dijumpai sepanjang saluran digestinya baik pada ikan yang berukuran kecil maupun besar. Aktivitas protease umumnya tinggi pada suasana asam dan netral pada usus depan dan tengah. Aktivitas protease ikan yang berukuran besar lebih rendah dari pada ikan yang berukuran lebih kecil. Aktivitas amilase tidak terdapat perbedaan diantara segmen usus yang diuji, namun aktivitas amilase tertinggi dijumpai pada ikan dengan berat rata-rata 13,29 g/ekor

Aktivitas Protease dan Komposisi Proksimat Tubuh Ikan Sidat (Anguilla bicolor Mcclelland) pada Kondisi Puasa dan Pemberian Pakan Kembali

Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Protease activity and body proximate composition of eel, Anguilla bicolor McClelland., under starvation and refeeding conditions with three treatments and four replications in randomized completely design has been evaluated. The result showed that the protease activity of eel was no significant different (P>.05) between treatment, but body proximate composition has significant differences (P<.05), especially in protein and lipid contents at starvation condition. It can be concluded that the eel protease activity did not change under starvation and refeeding condition, but the lipid content decreased at starvation condition.

Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture

Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

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Abstract

Eel population in nature reaches critical number, so that culture strategy is urgently needed to fulfil the high demand of this fish. A shortcut to get functional male, which proven difficult to be founded from natural catching, is masculinization. This research aimed to induce masculinization of tropical eel, Anguilla bicolor McClelland supplemented with various doses of 17α-methyltestosterone during a month culture in freshwater (0 ppt) or brackish water (10 ppt).  The eel was grouped and fed with supplementation of 17α-methyltestosterone at various doses, depending upon treatments, namely 0 mg Kg-1 diet (control), 40, 80 or 120 mg Kg-1 diet. Eels size were similar, at approximately 16,78 g ± 0,62 in weight and 25,38 cm ± 0,15 in length were either culture in freshwater or brackish during the experiment for eight weeks. Sex gonad, based on anatomical histological structures, Eye Index and Fin Index were measured after time culture treatment achieved, as well as body length, weight, eye diameter and the length of the pectoral fin were measured. Results showed that supplementation 17α-methyltestosterone 80 mg/Kg diet culture in brackish water has the highest number of male (90%).  This study proven that, the hormone was effective for masculinization in eels, It useful for masculinization in eels. Results proved that the 17α-methyltestosterone highly significant (P<0.01) effect on the Eye index (3.63 – 5.14) and Fin Index (3.03 – 4.08) of eels. This study concluded, that 17α-methyltestosterone more effective in improving the number of males in brackish water than in freshwater culture.

Reproductive characteristic of Anguilla bicolor McCleland , 1844 which inducted by administration of GnRh Analog

Jurnal Iktiologi Indonesia Vol 17, No 2 (2017): June 2017
Publisher : Masyarakat Iktiologi Indonesia (MII )

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Abstract

Eel, Anguilla bicolor McClelland was hard to mature in captivity, due to low levels of pituitary gonadotropin. In order to stimulate gonadal maturation in captivity, exogenous gonadotropin is needed. The purpose of this study was to assess the effect of GnRH-analog induction on gonadal maturation of tropical eel base on the value of the eye index, gonado-somatic index, and estradiol levels. The study was carried out experimentally with a completely randomized design with three treatments and eight replications. The treatments were the induction of hormone GnRH-analogues with doses of 0.0; 0.5 and 1.0 ml kg-1 body weight. Eel with an average total length of 67 cm and an average weight of 500 g were injected with different doses of GnRH-analog (0.0; 0.5 and 1.0 ml kg-1 body weight), kept in the fiberglass aquarium for two months. The eels were fed with discard fish daily in ad libitum at 16.00 pm. The observed variables were eye index, Gonadosomatic Index (GSI), and estradiol levels. The results showed that injection of three different doses of GnRH-analog did not affect on the increasing of GSI, eye index (P> 0.05), and estradiol (P <0.05). Induction of GnRH-analog of 0.0; 0.5 and 1.0 ml kg-1 body weight can not stimulate the gonadal maturity of tropical eel. AbstrakIkan sidat Anguilla bicolor McClelland sulit matang gonad dalam kondisi budi daya, karena rendahnya kadar gonado-tropin pituitari. Oleh karena itu untuk memacu pematangan gonad ikan sidat dalam kondisi budi daya perlu ditambah-kan gonadotropin secara eksogen. Tujuan penelitian ini adalah mengkaji pengaruh induksi GnRH-analog terhadap pe-matangan gonad ikan sidat berdasarkan perubahan nilai indeks mata, indeks kematangan gonad (IKG), dan kadar estradiol. Penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap dengan tiga perlakuan dan delapan ulangan. Perlakuan percobaan terdiri atas induksi hormon GnRH-analog dengan dosis 0,0; 0,5 dan 1,0 ml kg-1 bobot badan. Ikan sidat dengan rata-rata ukuran panjang 67 cm dan rata-rata bobot 500 g sebanyak 18 ekor yang telah diinduksi dengan berbagai dosis GnRH analog (0, 0,5 dan 1 ml kg-1 bobot badan) dipelihara dalam akuarium fiberglass selama dua bulan. Pakan berupa ikan rucah diberikan satu kali sehari pada pukul 16.00 secara ad libitum. Variabel yang diamati adalah indeks mata, IKG, dan kadar estradiol. Hasil penelitian menunjukkan bahwa induksi hormon GnRH-analog tidak berpengaruh terhadap peningkatan nilai indeks mata, nilai IKG (p>0,05), dan kadar estradiol ikan sidat (p<0,05). Induksi hormon GnRH-analog dengan dosis 0,5 dan 1 ml kg-1 bobot badan tidak efektif memacu pematangan gonad ikan sidat.

LEUKOCYTE DIFFERENTIAL OF ANGUILLID EEL, Anguilla bicolor McClelland, EXPOSED TO VARIED SALINITIES

Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

The anguillid eel is a catadromous eel capable of inhabiting freshwater growth habitat and seawater spawning habitat throughout their life cycle. At the juvenile to mature stage, they inhabit freshwater then migrate to marine water to spawn. Changes in salinity, which is one of the stressful environmental factors for the eel, affect their physiological condition by increasing the leukocytes number. This increase is an adaptation method to improve their immune system as a response to salinity change. This study intended to evaluate the leukocyte differential of anguillid eel (Anguilla bicolor McClelland) exposed to various salinities. This research applied a Completely Randomized Design. The treatment was three levels of saline media including 4 ppt, 15 ppt, and 30 ppt with five replicates. The independent variable was the different salinity, and the dependent variable was the leukocyte differential. The parameters measured consisted of the different percentage of neutrophils, lymphocytes, monocytes, and eosinophils in which the measurements administered after two months of the eel exposure. We analyzed the data with ANOVA at the confidence level of 95%. The results showed that exposure of salinity significantly affected the percentage of leukocyte differential (P < 0.05). The increase in salinity decreased the neutrophils and monocytes, but increased the lymphocytes, and showed no effect on eosinophils.

AKTIVITAS SUPEROKSIDA DISMUTASE TIKUS DIABETES YANG DIBERI EKSTRAK BATANG KAPULAGA DAN GLIBENKLAMID

Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Superoxide Dismutase (SOD) is an antioxidant enzyme which reduce anion superoxide radicals as well as known caused of diabetes. There are many natural additive was believed having capacity to repaired an antioxidant celluler status. Cardamom’s stem were reported containing flavonoid and vitamin C which have been proven as in vitro antioxidant. There was no data showing its in vivo potency. This study aims to knoe the SOD activity of diabetes rats which were given cardamom stem extract (CSE) and glibenclamide. The research carried out with the use of experimentally Randomized Design Complete (RAL) by administering treatment on diabetes rat without CSE and glibenclamide as a control, consist of 100 mg/kg bodymass CSE and 2 mg/kg bodymass glibenclamide. The experiment consists of 3 treatments with 7 repetitions, blood sampling carried out experiments as much as 3 times with intervals of 7 days once. The data was analyzed using a variety of analysis (ANOVA). The result showed that the SOD activity increased from 4261 Unit/mg protein to 6604,668 Unit/mg protein (P<0.01) in diabetes rats treatment by CSE for 14 days.

UKURAN ORGAN SISTEM REPRODUKSI ITIK JANTAN YANG DISUPLEMENTASI PROBIOTIK MEP+ BERBAGAI DOSIS SELAMA 30 HARI

Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Probiotics MEP+ can increase fowl weight and weft efficiency, therefore it is important to know probiotics MEP+ effect at different dosage toward reproduction aspect. This research aimed to examine duck reproduction organ size suplemented with probiotics MEP+ with different dosage within 30 days. This research used Completely Randomized Design (CRD) with 4 treatments with different dosages within 30 days which was without probiotic’s application or control (K), 0,75 ml/kg wefts (P1) dose, 1,5 ml/kg wefts (P2) dose, a n d 3 ml/kg wefts (P3) dose. Each treatment repeated 8 times. Total 40 ducks raised in floor dry cage system. At 31st day of treatment duck reproduction system organ was measured. Whole results show increase average data (±SD) for weight of both right and left testis, and liver weight with highly probiotics dosage it, however the analysis result statistic not significant (P>0,05) except weight of right left testis with duck weight or gonadosomatic indeks (GSI) were very significant (P<0,01) among all treatment at different dosages was compared control. The results is confirmed that probiotic's MEP+ treatment with different dosages within 30 days gave no effect towards duck reproduction system organ size except to gonadosomatic indeks (GSI) male duck.

PERUBAHAN KADAR PROTEIN DAN STATUS LIPOSTATIK IKAN SIDAT, Anguilla bicolor, STADIA SILVER YANG DIPELIHARA PADA SALINITAS YANG BERBEDA

Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Eel (Anguilla bicolor) is a catradomous fish, in the growth phase of life in freshwater and upon reaching adult will migrate to ocean waters to reproduce. Eel during the migration will use energy from body reserves to osmoregulation. Increased salinity will affect the osmotic pressure of the body of the fish so that the fish do active transport which requires energy. Energy that used for osmoregulation approximately 20–50% of the total energy from carbohydrates, fats and proteins. Carbohydrates are the first source of energy that is used and then will be replaced by fat and protein as energy after carbohydrate depleted. Thus the energy utilization during osmoregulation will affect the changes in the composition of protein and fat content of the body. Fat content will affect the value of lipostatic fish is one of the growth parameters. The purpose of this study was to evaluate changes in the levels of protein and lipostatic eel (Anguilla bicolor) is maintained in a range of salinity. This research method is experimental research design with CRD, treatment in the form of maintenance medium salinity; 4 ppt (control), 15 ppt, and 30 ppt. Each treatment was repeated 5 times. Test fish used were obtained from the Village Pesanggrahan Cilacap with range size between an average weight of 395–920 g and an average length of 59–81 cm. The variables measured were the levels of protein and lipostatic eel. Protein content measurement was conducted using kjeldahl and the fat content by soxhlet. Research results showed that the eels were exposed to salinity 4 ppt, 15 ppt and 30 ppt no effect on protein content and body lipostatic eel. It can be concluded that the eel is able to adapt well in the range between 4 ppt salinity to 30 ppt.