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PENGARUH MODEL PEMBELAJARAN Contextual Teaching And Learning (CTL) TERHADAP HASIL BELAJAR SISWA PADA POKOK BAHASAN SISTEM KOLOID Silaban, Saronom; Debora Simangunsong, Neeta Sri
JURNAL PENDIDIKAN KIMIA (JPKim) Vol 7, No 1 (2015): April 2015
Publisher : JURNAL PENDIDIKAN KIMIA (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v7i1.5507

Abstract

AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh model pembelajaran Contextual Teaching and Learning (CTL) terhadap hasil belajar siswa dalam pembelajaran kimia pada pokok bahasan sistem koloid. Penelitian ini telah dilakukan di SMA Methodist 1 Medan. Kelas sampel (kelas XI) diberikan perlakuan dengan model pembelajaran CTL. Kelas sampel telah memenuhi kriteria uji normalitas dan homogenitas. Evaluasi awal(pre-tes) siswa kelas sampel rata-rata M=16.25±6.25. Setelah perlakukan pembelajaran menggunakan model CTL, rata-rata pencapaian siswa (post-tes) meningkat menjadi M=76.39±6.09. Meningkatnya rata-rata post-tes siswa tersebut mengindikasikan bahwa pembelajaran menggunakan model CTL dapat meningkatkan hasil belajar siswa. Keberhasilan belajar ditandai dengan peningkatan hasil belajar siswa, dimana dalam perhitungan keberhasilan belajar berdasarkan %gain diperoleh rata-rata keberhasilan siswa sebesar 72% Hasil analisis uji beda atau uji t diperoleh thitung > ttabel (6,34> 1,6905) pada α = 0,05 yang berarti pembelajaran menggunakan CTL berpengaruh positif terhadap siswa dalam meningkatkan hasil belajarnya pada pokok bahasan sistem koloid.Kata Kunci: Pembelajaran CTL, sistem koloid, hasil belajar siswa
The Development of Chemical Practice Guidebook Colloid System-Based Integrated Contextual Character Values Manalu, Ester; Silaban, Saronom; Silaban, Ramlan; Hutabarat, Wesly
JURNAL PENDIDIKAN KIMIA (JPKim) Vol 8, No 2 (2016): Agustus 2016
Publisher : JURNAL PENDIDIKAN KIMIA (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v8i2.4429

Abstract

Abstract- This research is a development that aims to develop guiding chemistry lab contextually based integrated character values, knowing improving student learning outcomes in terms of cognitive and determine student learning outcomes in terms of affective and psychomotor using guiding chemistry laboratory that has been developed , The study was conducted in Cahaya Medan high school. Samples are two classes were taken by purposive sumpling using PPKBK experimental class I and class II experiment using PP available in school study instrument is a rubric validation, test and observation sheet. Research began on analytical chemistry lab at the school guidance, development, validation, revision and test laboratory guides that have been developed. The product of research is (1) based on the analysis of chemical PP book in colloidal system materials publisher grants, Pudak Scientific, Duta Nusantara and Laskar Aksara is not in accordance with BSNP, (2) based PPKBK BSNP integrated character values is more valid than the PP chemicals used in schools, 93) there is a difference in improving student learning outcomes in terms of cognitive, that learned to use the integrated PPKBK character values of 0.73, while that learned with chemicals used PP 0,63 school, (4) there are differences in student learning outcomes in terms of psychomotor aspect that learned to use PPKBK integrated character values at 91 and that learned with PP chemicals used school at 85, while in terms of affective aspects that learned to use PPKBK integrated character values of 92 and PP with chemicals schools at 81. Keywords: Development of practical guidance, based contextual character values, the colloid system
Codon Optimization and Chaperone Assisted Solubilization of Recombinant Human Prethrombin-2 Expressed in Escherichia coli SILABAN, SARONOM; MAKSUM, IMAN PERMANA; GHAFFAR, SHABARNI; HASAN, KHOMAINI; ENUS, SUTARYA; SUBROTO, TOTO; SOEMITRO, SOETIJOSO
Microbiology Indonesia Vol 8, No 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.8.4.5

Abstract

Prethrombin-2 (PT2) is a thrombin precursor, which plays a role in the conversion of fibrinogen into fibrin during blood clotting process. Previous study reported that the expression of human prothrombin-2 (rhPT2) in Escherichia coli formed inclusion bodies. The aim of this study was to establish a strategy to express a soluble rhPT2 in E. coli. This study was animed to design and codon optimize human prethrombin-2 gene as well as to optimize the expression condition using four strains of E. coli. The codon adaptation index (CAI) of the unoptimized hpt2 gene was 0.336, with 56.8% GC content. After optimization, the CAI of optimized hpt2 became 1.000 with 53.1% GC content. The optimized gene was successfully cloned into pTWIN1 expression vector. Expression analysis indicated that only E. coli ArcticExpress strain could successfully express a soluble recombinant rhPT2 protein, with only part of rhPT2 being expressed in insoluble form. However, the rest of the E. coli strains used in the experiments failed to express the rhPT2 in soluble form. We are deducing that the success in achieving soluble expression was not only due to the availability of chaperonins Cpn60/Cpn10, which played a crucial role in the protein folding in E. coli ArcticExpress strain, but also due to the codon optimization of hpt2 gene.
PENGEMBANGAN BAHAN AJAR INOVATIF RUMUS KIMIA DAN PERSAMAAN REAKSI BERBASIS MODEL PEMBELAJARAN PROBLEM BASE LEARNING (PBL) Silaban, Ramlan; Silaban, Saronom; Panggabean, Freddy T.M.; Ginting, Elsa
JURNAL PENDIDIKAN KIMIA (JPKim) Vol 7, No 1 (2015): April 2015
Publisher : JURNAL PENDIDIKAN KIMIA (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v7i1.5493

Abstract

Abstract The research aims to get an innovative chemistry teaching module on chemical formula and chemical equilibrium for X grade SMA  to improve student learning outcomes. This research conduct in some senior high school (SMA) in North Sumatera whose devide in two class, those  experiment and control class. This R&D type modifies from Borg&Gall theory, and some textbook contain this subject has been analyzing using BSNP criteria. The composed innovative teaching module trial on classroom by quasy-experiment methods. The experiment class taught using innovative teaching module and control class using textbook. The results showed that (1) Some chemistry textbook contain this subject which produced by some publisher has good quality but there are some sequences material and teaching strategy will be addition to completely them. (2) We have get a composing innovative teaching module on chemical formula and chemical equilibrium for X grade SMA on chemical formula and chemical equilibrium with combine PBL teaching model. This module have been standardization and fully criteria of BSNP. (3) There is a significant increasing of chemistry student learning outcomes using this innovative module on teaching classroom Keywords : development innovative teaching module; PBL model; learning outcomes
Aktivitas Antikanker Ekstrak Etanolbuah Ranti Hitam (Solanum blumei Nees ex Blume) Terhadap Sel Leukimia L1210 Simorangkir, Murniaty; Silaban, Saronom; Surbakti, Ribu; Barus, Tonel; Simanjuntak, Partomuan
Chimica et Natura Acta Vol 5, No 1 (2017)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v5.n1.12819

Abstract

Tanaman ranti hitam (Solanum blumei Nees ex Blumei), famili Solanaceae, ditemukan di daerah Dairi dan Karo, secara tradisional digunakan sebagai tanaman obat. Pengujian aktivitas antikanker dari ekstrak etanol buah ranti hitam terhadap sel leukemia L1210 telah dilakukan dengan metode hemositometri setelah diinkubasi 48 jam pada suhu 37°C di dalam inkubator 5% CO2. Hasil penelitian menunjukkan ekstrak etanol buah ranti hitam berpotensi sebagai antikanker terhadap sel leukemia L1210 dengan nilai IC50 sebesar 14,88 µg/mL (aktif).
Kajian Ekspresi Gen Pretrombin-2 Manusia Sintetik pada Escherichia coli Secara In Silico Untuk Produksi Trombin Sebagai Komponen Lem Fibrin Silaban, Saronom; Maksum, Iman Permana; Enus, Sutarya; Hasan, Khomaini; Subroto, Toto; Soemitro, Soetijoso
Jurnal Pendidikan Kimia (JPKim) Vol 8, No 1 (2016): April 2016
Publisher : Jurnal Pendidikan Kimia (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v8i1.4425

Abstract

Abstrak. Pretrombin-2 merupakan prekursor trombin dan dapat berperan sebagai komponen lem fibrin (LF). LF merupakan biomaterial perekat yang dapat diaplikasikan sebagai pengganti teknik jahitan pasca operasi. Biomaterial ini terdiri dari trombin, fibrinogen dan faktor XIII sebagai komponen utamanya. Dalam LF, trombin rekombinan berperan sebagai enzim yang mengubah fibrinogen menjadi fibrin untuk membentuk benang-benang fibrin sehingga luka tertutup. Kajian ini bertujuan untuk memprediksi ekspresi gen pretrombin-2 (PT2) manusia sintetik pada Escherichia coli secara in silico. Urutan asam amino pretrombin-2 manusia yang diperoleh dari data GeneBank dirubah ke dalam bentuk wild type urutan nukleotida menggunakan software OPTIMIZER sesuai dengan kodon preferensi E. coli K12, Selanjutnya dianalisis dengan Graphical Codon Usage Analyzer dan dioptimasi secara menual berdasarkan preferensi kodon E. coli dari data Codon Usage Database. Untuk memprediksi tingkat ekspresinya pada inang E. coli, dipelajari kajian ekspresi PT2 secara in silico menggunakan perangkat lunak dalam jaringan OptimumGeneTM. Hasil analisis secara in silico urutan nukleotida PT2 hasil optimasi dan wild type terhadap kodon preferensi E. coli menunjukkan bahwa, persentase rata-rata kandungan GC hasil optimasi sebesar 54,71% sedangkan wild type 52,56%, PT2 hasil optimasi memiliki 100% kodon dengan frekuensi tinggi, sedangkan wild type hanya 67%, dan PT2 hasil optimasi memiliki nilai Codon Adaptasi Index  satu, sedangkan wild type hanya 0,89. Dengan demikian dapat diprediksi bahwa gen pretrombin-2 manusia sintetik kemungkinan akan diekspresikan tinggi di inang E. coli. Kata kunci: pretrombin-2, trombin, kodon preferensi, kajian ekspresi, E. coli
Purification of Recombinant Human Pretrombin-2 in Escherichia coli for Thrombin Production as Fibrin Glue Components Silaban, Saronom; Maksum, Iman Permana; Hasan, Khomaini; Enus, Sutarya; Subroto, Toto; Soemitro, Soetijoso
Jurnal Pendidikan Kimia (JPKim) Vol 9, No 1 (2017): April 2017
Publisher : Jurnal Pendidikan Kimia (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v9i1.6201

Abstract

Abstract: Pretrombin-2 (PT2) is a thrombin precursor that plays a role in converting fibrinogen to fibrin for the process of wound recovery. This material can be applied instead of eye surgery suture technique. The intein-mediated refining system to purify the protein is attractive to be developed, since the protein is obtained by one purification step, capable of self-splicing, the protein can be diffused in to the N-terminal (PT2 cutting of the intein induced marker changes in pH and temperature) and on the C-terminal (cutting PT2 from the intein-induced marker of the thiol reagent). In this study, we purified the PT2 fusion of the expression of E. coli BL21 (DE3) Arctic Express in the intein-mediated chitin matrix column. PT2 was fused with a tag at its N-terminal position, containing the sequence of intein codes SspDnaB followed by chitin binder domain (CBD). Furthermore, the PT 2 fusion was expressed on the E. coli host, then purified in the chitin matrix column. The PT2 cutting process of the intein marker induced changes in the pH and temperature in the column. PT2 fusion was successfully purified in the intein-mediated chitin matrix. The PT2 fusion cut from the induced intein buffer marker at pH 6.5 and incubation at the temperature of 25 oC for 48 hours.Keywords: E. coli, expression, intein mediated purification, pH and temperature changes, PT2
PENGARUH PENGGUNAAN MEDIA ANIMASI KOMPUTER DAN MOTIVASI BELAJAR DALAM PEMBELAJARAN REAKSI REDOKS DAN ELEKTROKIMIA TERHADAP PRESTASI BELAJAR MAHASISWA Panggabean, Freddy T.M.; Silaban, Saronom
Jurnal Pendidikan Kimia (JPKim) Vol 7, No 1 (2015): April 2015
Publisher : Jurnal Pendidikan Kimia (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v7i1.5496

Abstract

Abstract This research aim to know how effect of computer animation instructional media and student motivation for student learning outcomes on teaching redox reaction. The quasy experiment methods conduct and achievement test using as instrument. Data was analysed using inferential statistic method. The results and hypothesis test shows: (1) there is a significant influence the use of computer animation media on learning redox reaction and electrochemical to achievement of students, in which chemistry learning achievement of students taught using computer animation media (84.38) is higher compared chemistry learning achievement of students who are taught without using the computer animation media (78.25), (2) there is a significant influence on learning motivation to achievement of students, in which chemistry learning achievement of students who have a high learning motivation (84.59) higher than chemistry learning achievement of students who have a low learning motivation (78.89), and (3) there is no significant influence of the interaction between the use of computer animation media and learning motivation of students to achievement of student. Kata kunci: Media Animasi, Motivasi, Prestasi Belajar, Kimia.
PENGARUH MODEL PEMBELAJARAN Contextual Teaching And Learning (CTL) TERHADAP HASIL BELAJAR SISWA PADA POKOK BAHASAN SISTEM KOLOID Silaban, Saronom; Debora Simangunsong, Neeta Sri
Jurnal Pendidikan Kimia (JPKim) Vol 7, No 1 (2015): April 2015
Publisher : Jurnal Pendidikan Kimia (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v7i1.5507

Abstract

Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh model pembelajaran Contextual Teaching and Learning (CTL) terhadap hasil belajar siswa dalam pembelajaran kimia pada pokok bahasan sistem koloid. Penelitian ini telah dilakukan di SMA Methodist 1 Medan. Kelas sampel (kelas XI) diberikan perlakuan dengan model pembelajaran CTL. Kelas sampel telah memenuhi kriteria uji normalitas dan homogenitas. Evaluasi awal(pre-tes) siswa kelas sampel rata-rata M=16.25±6.25. Setelah perlakukan pembelajaran menggunakan model CTL, rata-rata pencapaian siswa (post-tes) meningkat menjadi M=76.39±6.09. Meningkatnya rata-rata post-tes siswa tersebut mengindikasikan bahwa pembelajaran menggunakan model CTL dapat meningkatkan hasil belajar siswa. Keberhasilan belajar ditandai dengan peningkatan hasil belajar siswa, dimana dalam perhitungan keberhasilan belajar berdasarkan %gain diperoleh rata-rata keberhasilan siswa sebesar 72% Hasil analisis uji beda atau uji t diperoleh thitung > ttabel (6,34> 1,6905) pada α = 0,05 yang berarti pembelajaran menggunakan CTL berpengaruh positif terhadap siswa dalam meningkatkan hasil belajarnya pada pokok bahasan sistem koloid. Kata Kunci: Pembelajaran CTL, sistem koloid, hasil belajar siswa
The Development of Chemical Practice Guidebook Colloid System-Based Integrated Contextual Character Values Manalu, Ester; Silaban, Saronom; Silaban, Ramlan; Hutabarat, Wesly
Jurnal Pendidikan Kimia (JPKim) Vol 8, No 2 (2016): Agustus 2016
Publisher : Jurnal Pendidikan Kimia (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v8i2.4429

Abstract

Abstract- This research is a development that aims to develop guiding chemistry lab contextually based integrated character values, knowing improving student learning outcomes in terms of cognitive and determine student learning outcomes in terms of affective and psychomotor using guiding chemistry laboratory that has been developed , The study was conducted in Cahaya Medan high school. Samples are two classes were taken by purposive sumpling using PPKBK experimental class I and class II experiment using PP available in school study instrument is a rubric validation, test and observation sheet. Research began on analytical chemistry lab at the school guidance, development, validation, revision and test laboratory guides that have been developed. The product of research is (1) based on the analysis of chemical PP book in colloidal system materials publisher grants, Pudak Scientific, Duta Nusantara and Laskar Aksara is not in accordance with BSNP, (2) based PPKBK BSNP integrated character values is more valid than the PP chemicals used in schools, 93) there is a difference in improving student learning outcomes in terms of cognitive, that learned to use the integrated PPKBK character values of 0.73, while that learned with chemicals used PP 0,63 school, (4) there are differences in student learning outcomes in terms of psychomotor aspect that learned to use PPKBK integrated character values at 91 and that learned with PP chemicals used school at 85, while in terms of affective aspects that learned to use PPKBK integrated character values of 92 and PP with chemicals schools at 81. Keywords: Development of practical guidance, based contextual character values, the colloid system