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Safe Food Handling Knowledge, Attitude and Practice of Food Handlers in Hospital Kitchen Lestantyo, Daru; Husodo, Adi Heru; Iravati, Susi; Shaluhiyah, Zahroh
International Journal of Public Health Science (IJPHS) Vol 6, No 4: December, 2017
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v6i4.10778

Abstract

Hospital food hygiene plays an important role to patient's health. Studies proved that improper practices and lack of knowledge are contributing factors of foodborne diseases. This study was conducted to determine knowledge, attitude and practice of hospital safe food handling in Semarang, Central Java. A cross sectional study was conducted in two general hospitals' kitchen. Data were collected from 60 food handlers through validated questionnaire. Respondents were grouped into two categories: a. Teaching Hospital, and b.Non-teaching Hospital. Data were collected in February-April 2017. Most of food handlers (80%) were graduated from vocational senior high school (SMK). Respondent's age were 28-37 years (36.7%) in average. Most of respondent‟s work experience (85%) was less than 15 years. The result showed that more than 80% of food handlers have good knowledge in safe food handling procedures, good attitude (66%) and good practices (90%). There was no significant difference of knowledge, attitude and practices between two groups. Results strongly emphasize the need of food hygiene training of hospital food handlers. It also suggest that non food handlers such as nurse or kitchen supervisor should involved in the training. Some aspects of food handlers' safety behavior need to be emphasized.
Angka kuman di ruang rawat inap RSUD Dr. M. Haulussy Ambon Provinsi Maluku Ningsih, Tina Amnah; Iravati, Susi; Nuryastuti, Titik
Berita Kedokteran Masyarakat (BKM) Vol 32, No 6 (2016)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.3 KB) | DOI: 10.22146/bkm.8763

Abstract

The number of bacteria in the inpatient rooms of RSUD Dr. M. Haulussy Ambon HospitalPurpose The purpose of this study was to determine the total number of germs and identify factors related to the number of germs in the inpatient wards of RSUD dr. M. Haulussy Ambon Hospital.MethodsThis observational study used a cross-sectional design. The space objects used for samples were internal wards, surgical wards, and neurological wards of RSUD. Dr. M. Haulussy Ambon.ResultsThe temperature had a significant relationship with the number of morning air germs. Lighting had a significant relationship with the numbers of germs in the afternoon air and on the afternoon floor.ConclusionsThis study showed that the number of germs in the air, on the walls, and floors in the hospital wards of Dr. M. Haulussy Ambon did not fulfil the requirements according to Kepmenkes no. 1204/Menkes/SK/X/2004. It is necessary to improve the quality of temperature and lighting in order to reduce the number of germs in hospital wards.
ANGKA KUMAN UDARA DAN LANTAI RUANG RAWAT INAP RUMAH SAKIT PKU MUHAMMADIYAH YOGYAKARTA Wulandari, Windi; Sutomo, Adi Heru; Iravati, Susi
Jurnal Berkala Kesehatan Vol 1, No 1 (2015): JURNAL BERKALA KESEHATAN
Publisher : Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.637 KB) | DOI: 10.20527/jbk.v1i1.655

Abstract

                                                              Abstract One of the objectives of health development is a healthy environment including hospital environment. The quality of hospital environment becomes one of that needs to be considered, because of there are some ways of transmission of germ that causing infection could occured through droplet, airborne or direct contact. The spreading of nosocomial infections in hospitals may occur at existing facilities in hospital such as theatre or surgery room, emergency room, outpatient installation, and patient room. To determine the factors those are related to the number of bacteria in the air and floor of inpatient room of PKU Muhammadiyah Hospital Yogyakarta. The study was an observational analytic cross-sectional study design. The populations in this study were all of inpatient rooms at PKU Muhammadiyah Hospital of Yogyakarta. The samples of the observation were all five wards. Data were collected using a check list, measuring temperature, humidity, lighting, number of bacteria in air and floor. Data were analyzed using Pearson correlation test to examine the relationship between variables and using Anova to see the differences. The results showed a significant relationship exists between the number of patients with air bacteria rate (p = 0.037, r = 0.900), there is no correlation between the number of waiters and the number of air bacteria amount (p = 0.505, r = 0.400), there is correlation between the number of visitors and the number of air bacteria (p = 0.037, r = 0.900), there is no correlation between the number of air bacteria of sanitary room (p = 1.000, r = 0.000), there is no correlation betwen the number of patients with floor bacteria rate (p = 0.283, r = -0.602), there is no correlation between the number of waiters with the number of floor bacteria rate (p = 0.420, r = -0.474), there is correlation between the number of visitors to the number of floor bacteria (p = 0.032, r = -0.910), there is no correlation between sanitation floor space with a number of bacteria (p = 0.991, r = -0.007), there is no difference between the number of bacteria by the day (p = 0.82, F = 2.121), there is no difference in the number of floor bacteria by day (p = 0.226). There is a significant correlation between the number of patients and the number of visitors to the average air bacteria rate per week in patient room. The number of patients and the number of visitors are the most powerful variable correlation contributed to the number of bacteria in the air in patient room PKU Muhammadiyah hospital Yogyakarta. Keywords: rate of air bacteria, floor bacteria numbers, inpatient wards, sanitation, hospitals
SUSU KUDA SUMBAWA KHAS INDONESIA BAHAN KOSMETIK ANTIBAKTERI JERAWAT (Staphylococcus epidermidis) Setyowati, Erna Prawita; Laili, Fauzia Nur; Iravati, Susi
Majalah Obat Tradisional Vol 19, No 2 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/mot-TradMedJ19iss2pp74-79

Abstract

Susu kuda Sumbawa merupakan salah satu susu murni hasil perahan kuda liar yang diternak oleh petani organik di pulau Sumbawa, Nusa Tenggara Barat, Indonesia. Masyarakat ramai mengklaim susu ini mempunyai banyak khasiat. Pada penelitian ini susu kuda Sumbawa dibuktikan efektif sebagai antibakteri terhadap Staphylococcus epidermidis (salah satu bakteri penyebab jerawat). Uji aktivitas antibakteri dilakukan dengan metode spread plate  dan dihitung dengan standard plate count. Presentase jumlah koloni bakteri uji yang mati setelah kontak dengan susu kuda Sumbawa selama 5 menit (dari kontrol asam) pada pengenceran 25% sebesar 99,96%, pengenceran 12,5% sebesar 99,99%, pengenceran 6,25% sebesar 99,67% dan pengenceran  3,125 % sebesar 99,73 %. Hal ini menunjukkan bahwa susu kuda Sumbawa mempunyai aktivitas antibakteri yang cukup baik sampai konsentrasi 3.125%.
Detection of eae, bfpA, espA Genes on Diarrhoeagenic Strains of Escherichia coli Isolates Harti, Agnes Sri; Iravati, Susi; Asmara, Widya
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

The Enteropathogenic Escherichia coli (EPEC) is one of pathogenic strain of diarrheagenic E. coli group in children andinfant that occurs in developing countries. The significant virulence factors in pathogenic EPEC are eaeA (E. coli attachingeffacing), bfpA (bundle-forming pilus A) and espA (encoding secreted protein A) genes. The use of DNA probes to detect thevirulence genes in E. coli in Indonesia is not common yet. In this experiment the gene fragments of eae, bfpA, and espA were usedas probes to detect the EPEC among E. coli isolates from stool specimensin of diarrheic children attending Public Health Centersin Yogyakarta. The DNA samples were isolated from 49 diarrheagenic E. coli isolates. The DNA probes of eae, bfpA and espAwere obtained by amplification of DNA fragment of EPEC O126 using PCR technique. Furthermore, those probes were used toidentify the presence of those genes among E. coli isolates using hybridization technique. The results showed that 42 (85.7%)isolates were espA+, 25 isolates (51%) were eaeA+ (EPEC strains). Therefore among 25 isolates of EPEC, 20 isolates (80 %)among EPEC were bfpA+ (typical EPEC strains).Keywords : DNA probe, eae, bfpA, espA, EPEC.
PENURUNAN TOKSISITAS LEACHATE (AIR LINDI) DARI TPAS PUTRI CEMPO MOJOSONGO SURAKARTA DENGAN PAC (POLY ALUMINUM CHLORIDE) Astuti, Dwi; Sarto, Sarto; Iravati, Susi
Jurnal Manusia dan Lingkungan (Journal of People and Environment) Vol 17, No 1 (2010)
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

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Abstract

Tujuan penelitian ini adalah menetapkan persen penurunan toksisitas sesudah diperlakukan dengan PAC. Rancangan penelitian ini adalah eksperimen murni dengan pretest-posttest with control group design. Populasi dalam penelitian ini adalah air lindi yang berasal dari TPAS Putri Cempo M<rjosongo Surakarta. Sampel penelitian berupa air lindi sebanyak 250 liter yang diambil dari bagian outlet, cara pengambilan sampel dengan metode quota sampling. Hasil menunjukkan bahwa toksisitas air lindi terhadap ikan uji berdasarkan LC50 24-96 jam sebagai berikut: (1) tanpa perlakuan PAC: 25,06% (24 jam); 21,07% (48 jam); 17,49% (72 jam); dan 14,97% (96 jam). (2) Dengan perlakuan PAC: 89,44% (24 jam); 63,73% (48 jam); 49,99% (72 jam); dan 40,96% (96 jam). Sehingga persentase penurunan toksisitasnya adalah: 64,38% (24 jam), 42,66% (48 jam), 32,50% (72 jam), dan 25,99% (96 jam). 
Detection of eae, bfpA, espA Genes on Diarrhoeagenic Strains of Escherichia coli Isolates Harti, Agnes Sri; Iravati, Susi; Asmara, Widya
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.7563

Abstract

The Enteropathogenic Escherichia coli (EPEC) is one of pathogenic strain of diarrheagenic E. coli group in children andinfant that occurs in developing countries. The significant virulence factors in pathogenic EPEC are eaeA (E. coli attachingeffacing), bfpA (bundle-forming pilus A) and espA (encoding secreted protein A) genes. The use of DNA probes to detect thevirulence genes in E. coli in Indonesia is not common yet. In this experiment the gene fragments of eae, bfpA, and espA were usedas probes to detect the EPEC among E. coli isolates from stool specimensin of diarrheic children attending Public Health Centersin Yogyakarta. The DNA samples were isolated from 49 diarrheagenic E. coli isolates. The DNA probes of eae, bfpA and espAwere obtained by amplification of DNA fragment of EPEC O126 using PCR technique. Furthermore, those probes were used toidentify the presence of those genes among E. coli isolates using hybridization technique. The results showed that 42 (85.7%)isolates were espA+, 25 isolates (51%) were eaeA+ (EPEC strains). Therefore among 25 isolates of EPEC, 20 isolates (80 %)among EPEC were bfpA+ (typical EPEC strains).Keywords : DNA probe, eae, bfpA, espA, EPEC.
Scientific Aspects of Water Extract Katola (Arcangelisia flava L. Merr) as Antidiarrhea Agent in Mina South Sulawesi Larisu, Muhammad Akhram; ., Sudarsono; Iravati, Susi; Nurrochmad, Arief
INDONESIAN JOURNAL OF PHARMACY Vol 21 No 4, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.881 KB) | DOI: 10.14499/indonesianjpharm0iss0pp283-289

Abstract

Water  extract  of  katola  (Arcangelisia  flava  L.  Merr)  stem  is  traditionally used in communities Muna Distric for the treatment of many diseases, including anti  diarrhea.  According  to  the  information  of  Muna  Distric  Health  Office, diarrhea  is  one  of  the  10  groups  diseases  in  this  district.  The  objective  of  this study was conducted to determine the potential antimicrobial, acute toxicity and identified  of  the  water  extract  of  bioactive  compounds  and  alkaloid  relative concentration content.  Infection of microbial was induced by oral administration of  Shigella  flexneri  ATCC  12022  1  x  108 CFU/mL.  Boiling  water  of  katola  dose 12  mg/kg,  24  mg/kg  and  48  mg/kg  body  weight  and  the  antibiotic  ampicillin 24  mg/kg  administered  2  times  daily  for  5  days.  The  number  of  S.  flexneri  in feces were determined every day. The toxicity of water extract of  katola stem  is determined  based  on  the  value  of  LD50  acute  oral  toxicity  testing  followed  by guidelines  OECD  423. The  bioactive compounds was determined by bioautografi method  and  the  concentration  alkaloid  relative  content  was  calculated  as berberine  hydrochloride.  The  results  showed  that boling  water extract of  katola stem  did  not  cause  toxic  symtoms,  with  LD50  >  31.5  g/kg  body  weight (unclassified)  or  104  times  the  dose  therapy  of  human.  In  vitro,  the  Minimum Inhibition  Concentration  (MIC)  and  Minimum  Bactericidal  Concentration (MBC)value  of  the  boiling  water  were  1.2  and  2.4%,  respectively.  In  vivo,  the boiling  water  dose  48  mg/kg  body  weight  given  twice  a  day  have  an  effect  on eradication the S. flexneri 100% by day 5. Bioactive compounds of antimicrobial in  the  water  extract  is  alkaloid  berberine  HCl.    A  cocentration  of  berberine hydrochloride  in  water  exract  stem  and  leaves  dosage  use  of  the  public  were 16.7% and 0.75% respectively.Key words: Katola, toxicity, anti diarrhea, antimicroba, Arcangelisia flava L. Merr 
Faktor iklim dan kondisi fisik lingkungan rumah dengan kejadian demam berdarah dengue di beberapa zone season: studi kasus di Bantul, Yogyakarta Ayumi, Farid; Iravati, Susi; Umniyati, Sitti Rahmah
Berita Kedokteran Masyarakat (BKM) Vol 32, No 12 (2016)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.876 KB) | DOI: 10.22146/bkm.8790

Abstract

Climate factors and conditions of physical environment house for the dengue fever incidence in several zone season in: a case study from Bantul, YogyakartaPurposeThis research aimed to determine the relationship between climate (rainfall, air humidity, and temperature) and physical conditions of house environment with incidence of dengue fever in some seasonal zones in Yogyakarta.MethodsThe design of this study was an ecological study by time to observe the trend of dengue incidence in Yogyakarta within the period of 2010-2014.ResultsResults showed not all ZOMs were related to the incidence of dengue fever in Yogyakarta. Environmental conditions of the physical house that have a correlation with the dengue fever were breeding place outside, while the use of gauze ventilation and the existence of mosquito larvae in-house, were not correlated to an occurrence of dengue fever.ConclusionRainfall, air humidity, and temperature have a correlation with the incident of dengue fever in some ZOM area. The condition of the physical environment of the house in the form of breeding places outside of the house is associated with the occurrence of dengue fever. 
SUSU KUDA SUMBAWA KHAS INDONESIA BAHAN KOSMETIK ANTIBAKTERI JERAWAT (Staphylococcus epidermidis) Laili, Fauzia Nur; Setyowati, Erna Prawita; Iravati, Susi
Majalah Obat Tradisional Vol 19, No 2 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tradmedj.8144

Abstract

Susu kuda Sumbawa merupakan salah satu susu murni hasil perahan kuda liar yang diternak oleh petani organik di pulau Sumbawa, Nusa Tenggara Barat, Indonesia. Masyarakat ramai mengklaim susu ini mempunyai banyak khasiat. Pada penelitian ini susu kuda Sumbawa dibuktikan efektif sebagai antibakteri terhadap Staphylococcus epidermidis (salah satu bakteri penyebab jerawat). Uji aktivitas antibakteri dilakukan dengan metode spread plate  dan dihitung dengan standard plate count. Presentase jumlah koloni bakteri uji yang mati setelah kontak dengan susu kuda Sumbawa selama 5 menit (dari kontrol asam) pada pengenceran 25% sebesar 99,96%, pengenceran 12,5% sebesar 99,99%, pengenceran 6,25% sebesar 99,67% dan pengenceran  3,125 % sebesar 99,73 %. Hal ini menunjukkan bahwa susu kuda Sumbawa mempunyai aktivitas antibakteri yang cukup baik sampai konsentrasi 3.125%.