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Developing of Tris Soy Milk Diluent for Frisian Holstein Bull Frozen Semen ARIFIANTINI, RADEN IIS; YUSUF, TUTY LASWARDI
HAYATI Journal of Biosciences Vol 17, No 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (33.15 KB) | DOI: 10.4308/hjb.17.2.91

Abstract

Commercial artificial insemination (AI) companies in the beginning of year 2000, introduced new generation of cryoprotectans with lecithin based diluents such as Biociphos (IMV, L’Aigle, France) and Andromed®  (Minitub, Germany). Since the commercial diluents were imported, they were often not readily available. This research aimed to develop Tris-soy modified diluent, and investigated its effects on the quality and fertility of Frisian Holstein (FH) bulls frozen semen. This research consists of two experiments. At first, we compared the FH Bull frozen semen quality in modified tris soy milk (TSM), Andromed® and Tris-eggyolk (TEY) diluents, the second  was the fertility trials of the frozen semen. Results of the experiment demostrated that post thawed sperm motility in the semen preserved with TEY (49.10%) or Andromed® (50.21%) was significantly higher (P = 0.037) than that preserved with TSM (41.53%). In contrast, the conception rates in cows inseminated with semen preserved with TSM, TEY, and Andromed® were 53.84% (7/13), 38.88% (5/13), and 38.46% (7/19), respectively. We are optimistic that TSM diluents will have similar qualities as TEY and Andromed® on preserving frozen semen by doing future intensive studies.
Diurnal Patterns of Testosterone and Cortisol Metabolites in Fecal of Javan Gibbons (Hylobates moloch) in Captivity ASTUTI, PUDJI; YUSUF, TUTY LASWARDI; HAYES, ERIC; MAHESHWARI, HERA; SJAHFIRDI, LUTHFIRALDA; SAJUTHI, DONDIN
HAYATI Journal of Biosciences Vol 13, No 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (78.12 KB) | DOI: 10.4308/hjb.13.2.69

Abstract

The aims of this research were to determine diurnal patterns of testosterone and cortisol metabolites to predict the testis functional status. In this study, fecal testosterone and cortisol were quantified in 77 samples from three male Hylobates moloch during a course of three months period. These data showed that the highest concentration of fecal testosterone occured at 18.00-06.00 (23.61 ng/g dried feces), then declined gradually. The lowest concentration was in the evening (5.54 ng/g dried feces). Our tests showed that there was a decrease in the mean testosterone concentration from 06.00-10.00 to 10.00-14.00 to 14.00-18.00. For cortisol, the highest concentration occured at 06.00-10.00 (597.84 ng/g dried feces), then decline gradually in the evening (225.73 ng/g dried feces). Key words: Hylobates moloch, feces, testosterone, cortisol, diurnal pattern
Sinkronisasi Estrus dan Inseminasi Buatan pada Rusa Timor Nalley, Wilmientje Marlene Mesang; Handarini, Ristika; Arifiantini, Raden Iis; Yusuf, Tuty Laswardi; Purwantara, Bambang; Semiadi, Gono
Jurnal Veteriner Vol 12, No 4 (2011)
Publisher : Jurnal Veteriner

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Abstract

The objective of this experiment was to examine the fertility of timor deer (Rusa timorensis) frozensemen by artificial insemination (AI) with intracervical technique after estrus synchronization withControlled Internal Drug Release, for Goat (CIDR-G®). Six adult, healthy, and cycling hinds aged 3 to 4years were used in this experiment. The percentage of estrus hind, onset and duration of estrus wereobserved. Pregnancy diagnosis was conducted using ultrasound scanner (USG) at day 120 afterinsemination. The response of estrus was 82.22 %, the onset of estrus was 25.33 hours after CIDR®withdrawal with the length of estrus was 28 hours. The pregnancy rate at first AI trial under sedation was16.7% (1/6), at second and third without sedation was 60% (3/5) and 100% (2/2) respectively. The durationof pregnancy was 248 to 285 days; with the average calving rate was 50%. The result concludes thatinsemination in hind without sedation was better than with sedation.
The Use of Direct Transfer Method on Embryo Cryopreservation in Dairy Cattle Supriatna, Iman; yusuf, Tuty Laswardi; Purwantara, Bambang; Moekti, Gozali; Hernomoadi, Lies Parede
Media Veteriner Vol 6, No 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiment was carried out to study the use of direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-fourmorula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0,8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.5 M sucrose were 80.0, 80.8, 90.9 and 81.80% respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0,8 M (90.0%) sucrose was significantly better compared to those without (22.2%) or with 0.2 M (36.3%) sucrose (p
Study on The Application of Human Chorionic Gonadotropin (hCG) in Dairy Cow Superovulated With Pregnant Mare Serum Gonadotropin - Monoclonal Antibody (PMSG-MoAb) Anti PMSG. Supriatna, Iman; yusuf, Tuty Laswardi; Purwantara, Bambang; Moekti, Gozali; Hernomoadi, Lies Parede
Media Veteriner Vol 5, No 2 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The researcb was planned to yield the biologic potential synergism of MoAb-bCG in increasing the embryo number. Twenty five dairy cows were divided into five groups. The Control Group was superovulated witb 2,500 IU PMSG intramuscularly (i.m.), the remaining of the Group II, III, IV and V consecutively after being superovulated by 2,500 IU PMSG were treated with MoAb intravenously (i.v.), hCG i.v., MoAb-hCG Lv. and MoAb i.v-hCG i.m. Data evaluation were analyzed using ANOV A and LSD test. The result showed that there were increasing in number of transferable embryo per donor produced (P0,05).
Morfologi Kelenjar Aksesori Kelamin Muncak (Muntiacus muntjak muntjak) Jantan Wahyuni, Sri; Manik, Lidya Elizabeth M.; Agungpriyono, Srihadi; Agil, Muhammad; Yusuf, Tuty Laswardi; Hamny, .; Adnyane, I Ketut Mudite
ACTA VETERINARIA INDONESIANA - Indonesian Veterinary Journal Vol 1, No 2 (2013): Juli 2013
Publisher : Bogor Agricultural University

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Abstract

Penelitian ini bertujuan untuk mempelajari morfologi kelenjar aksesori muncak jantan secara makroanatomi dan mikroanatomi. Seekor muncak jantan dewasa berumur 4-5 tahun dengan bobot badan 19 kg digunakan pada penelitian ini. Muncak terlebih dahulu di-exanguinasi untuk dikoleksi kelenjar aksesori kelaminnya. Untuk memperoleh gambaran mikroanatomi, sampel kelenjar aksesori diproses dengan teknik histologi dan diwarnai dengan pewarnaan hematoksilin-eosin (HE). Hasil pengamatan makroskopis menunjukkan bahwa kelenjar aksesori muncak jantan terdiri atas ampula, duktus deferens, kelenjar prostat, kelenjar vesikularis, dan kelenjar bulbouretralis. Karakteristik histologi kelenjar aksesori muncak adalah ditemukannya kelenjar prostat yang berbentuk pars diseminata dengan kelenjar-kelenjar sekretori tersebar di sekeliling lumen uretra pars pelvina dimana secara makroskopis kelenjar tersebut tidak dapat diamati. Tipe kelenjar sekresi pada ampula, kelenjar vesikularis, dan pars diseminata prostat adalah tubuloalveolar, sedangkan pada kelenjar bulbouretralis tipe tubular. Dapat disimpulkan bahwa morfologi kelenjar aksesori muncak jantan memperlihatkan kemiripan dengan kelenjar aksesori pada ruminansia kecil lainnya seperti kambing, domba, reeves muntjak, dan pampas deer.
Dinamika Ovarium Selama Siklus Estrus pada Domba Garut (OVARIAN DYNAMIC DURING THE ESTROUS CYCLE IN GARUT EWES) Gunawan, Satya; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus; Boediono, Arief; Herman, Rachmat; ., Amrozi
Jurnal Veteriner Vol 13, No 2 (2012)
Publisher : Jurnal Veteriner

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Abstract

Ovarian dynamics in the garut ewes had never been studied continuously by using ultrasonography.The aim of this study was to observe development of the follicles and corpus luteums in the estrous cyclein the garut ewes. Garut ewes (n=6) with body weight 30.00±4.05 kg which had normal estrous cycle wereused in this study. All ewes were synchronized by using CIDR-G implantation for 14 days. Ovulation of thedominant follicle, development of the follicle waves and corpus luteum were observed continuously everyday during the estrous cycle after CIDR-G removal. The number of small (2-3 mm in diameter), medium (4-5 mm in diameter) and large (>5 mm in diameter) follicles were aligned during the estrous cycle. Follicleand corpus luteum diameters were measured by using built in caliper in the ultrasound. The resultsshowed 1) the average length of estrous cycle was 19,2±0,8 days; 2) ovarian follicle growth occurred inthree waves during the estrous cycle; 3) the number of preovulatory follicles were 1-2 follicles; 4) theaverage maximum diameters of preovulatory follicle was 7.5±0.5 mm; 5) the average maximum diametersof corpus luteum was 7.3±0.4 mm. In conclusion, the estrous cycle in garut ewes was 18-20 days with 3follicular waves.
Morfometri, Morfologi Serta Abnormalitas Spermatozoa Owa Jawa (Hylobates moloch) Asal Ejakulat Astuti, Pudji; Yusuf, Tuty Laswardi; Sajuthi, Dondin; Hayes, Eric; Budipitojo, Teguh; Sjahfirji, Luthfiralda; Maheshwari, Hera
Jurnal Sain Veteriner Vol 22, No 2 (2004)
Publisher : Fakultas Kedokteran Hewan

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Abstract

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Kualitas Semen Cair Kambing Peranakan Etawah dalam Modifikasi Pengencer Tris dengan Trehalosa dan Rafinosa (THE QUALITY OF ETAWAH CROSSBREED BUCK LIQUID SEMEN IN MODIFIED TRIS DILUENTS WITH TREHALOSE AND RAFFINOSE) Ariantie, Oriza Savitri; Yusuf, Tuty Laswardi; Sajuthi, Dondin; Arifiantini, Raden Iis
Jurnal Veteriner Vol 15, No 1 (2014)
Publisher : Jurnal Veteriner

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Abstract

The aim of this study was to compare the efficacy of trehalose and raffinose supplementation in Trisegg yolk (TEY) and Tris soya (TS) diluents in optimizing the quality of Etawah Crossbreed liquid semen.Semen were collected from three sexually mature bucks using artificial vagina. Semen were then evaluatedand divided into six aliquot tubes. Each of them was diluted in TEY and TS supplemented with 50 mMtrehalose or raffinose, respectively. The liquid semen were then stored in refrigerator (5°C). The motility,viability and plasma membrane integrity (PMI) of the spermatozoa were evaluated every 12 hours untilsperm motility remained up to 50%. The results showed that sperm motility in TEY supplemented withtrehalose or raffinose remained up to 50% for 72-84 hours, compared to in TS which was for 48-60 hours(P<0,05). The best diluent was demonstrated by TEY supplemented with trehalose where the spermmotility was 52,82±3,21% up to 84 h compared to raffinose supplementation (52,78±4,41%) and control(51,78±4,86%) which was up to 72 hours, respectively. Meanwhile, the spermatozoa motility in TS diluentsupplemented with raffinose was 52,78±4,41% for up to 60 hours compared to supplemented with trehalose(53,33±3,54%) and control (51,11±4,86%) which was up to 48 hours. In all diluents, the viability ofspermatozoa was 6-9% higher than the percentage of sperm motility, whilst the percentage of PMI wassimilar to the percentage of sperm motility. In conclusion, TEY supplemented with trehalose was the bestdiluents for preservation of Etawah Crossbreed buck liquid semen, and when using TS diluent it isrecommended to add raffinose  rather than trehalose.
Penentuan Waktu Optimal Kawin Berdasarkan Ultrasonografi Ovarium dengan Gejala Klinis Estrus pada Kambing Peranakan Etawa (DETERMINATION OF OPTIMAL MATING TIME BASED ON OVARY ULTRASONOGRAPHY WITH ESTROUS CLINICAL SYMPTOMS IN ETAWA CROSSBREED GOAT) Satria, Yudi Eka; Yusuf, Tuty Laswardi; ., Amrozi
Jurnal Veteriner Vol 17, No 1 (2016)
Publisher : Jurnal Veteriner

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Abstract

The aim of this study was to determine the optimal mating time in Etawa Crossbred Goat. This studyused 10 ewes, 2-3 years old, with normal estrous cycle. Initiation of estrous using Controlled Internal DrugRelease Implants (CIDR) intra vaginal and released after 12 days. The development of follicles in theovaries wasobserved using transrectal Ultrasonography (USG) every six hours until reach for 66 hoursafter CIDR removal from the vagina. Clinical signs of standing heat and vulva signs were also observedwhile under USG observation. The follicles were divided into three groups based on their diameter:small(<3 mm), medium (4-4.9 mm) and large (>5 mm) which were observed during theestrous phase. Ovulationwas predicted when large follicles (dominant follicles) havenot been seen anymore. The resulta showedthat the average follicles number in ovary after 36 hours CIDR removal were 0-1 (0.8±0.4 mm) small, 0-1(0.9±0.6 mm) medium and 1-2 (1.7±0.8 mm) large, respectively. The large follicles (7-8 mm) wereseen after36 hours CIDR removal or 24 hours after standing heat. Forty two hours after CIDR removal, the largefollicles werenot being seen anymore. It means, the ovulation were predicted in 36-42 hours after CIDRremoval or 24-30 hours after standing heat. The relationship between follicular development and clinical vulva signs (redness, swelling, mucus) revealed that the highest vulva signs (+++) wereseen in 36-42 hoursafter CIDR removal. It was concluded that the ovulation time occurred in 36-42 hours after CIDR removal,with the high intensity of vulva changes. Therefore the optimal mating time in PE 24-36 hours of standingheat or 36-42 hours after CIDR removal.