Muhamad Yunus
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor 16111 Phone (62-251) 8337975

Published : 6 Documents
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Evaluasi Daya Gabung dan Heterosis Lima Galur Jagung Manis (Zea mays var. saccharata) Hasil Persilangan Dialel

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

  The objective of this research was to estimate general combining ability (GCA), specific combining ability (SCA), and heterosis of five sweet corn inbreds in order to be selected to develop hybrid varieties. Five inbred lines as parent were Mr12/SC/BC4-6-1B-1, Mr14/SC/BC4-6-1B-1, Mr4/SC/BC4-2-1B-1, Mr11/SC/BC4-2-1B-1, and Mr12/SC/BC3-3-1B-1. The experiment was conducted from April to June 2010 using a Randomized Complete Block Design with two replications at Indonesian Cereal Research Institute (ICERI) experimental station, Maros, South Sulawesi. Analyses of GCA and SCA were based on the Griffing’s fixed model of Diallel Design Method I. Heterosis values were predicted based on the average values of their parents whereas heterobeltiosis were predicted based on the average values of the highest parents. Cross B x D (Mr14/SC/BC4-6-1B-1 x  Mr11/SC/BC4-2-1B-1) had  the highest SCA for yield 990.67. Cross A x B (Mr12/SC/BC4-6-1B-1 x Mr14/SC/BC4-6-1B-1) had the highest SCA for ear diameter 0.36. Cross A x D (Mr12/SC/BC4-6-1B-1 x Mr11/SC/BC4-2-1B-1) had the highest heterosis and heterobeltiosis for yield, while Cross A x D (Mr12/SC/BC4-6-1B-1 x Mr11/SC/BC4-2-1B-1) had the highest heterosis and heterobeltiosis for ear  length. Cross A x B (Mr12/SC/BC4-6-1B-1 x Mr14/SC/BC4-6-1B-1) and E x A (Mr12/SC/BC3-3-1B-1 xMr12/SC/BC4-6-1B-1) had the highest heterosis and heterobeltiosis for ear diameter.   Keywords: combining ability, diallel crossing, heterosis, sweet corn 

Phylogenetic and Maturity Analyses of Sixty Soybean Genotypes Used for DNA Marker Development of Early Maturity Quantitative Trait Loci in Soybean

Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
Publisher : Jurnal AgroBiogen

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Abstract

The Indonesian soybean productivity is still very low with the national average of 1.3 t/ha. One means to improve national soybean productivity is by manipulating harvest index by cultivating very early maturing soybean cultivars. Development of early maturing soybean cultivars can be expedited by using marker-aided selection. The objective of this study was to select parental lines having contrasted maturity traits and selected parents must be genetically distance. The parents then were used to develop F2 populations for detecting early maturity QTL in soybean. Maturity tests of 60 soybean genotypes were conducted at two locations, Cikeumeuh (Bogor) and Pacet (Cianjur) using a randomized block design with three replications. Genomic DNA of the 60 genotypes were analyzed using 18 SSR markers and genetic relationship was constructed using the Unweighted Pair-Group Method Arithmatic through Numerical Taxonomy and Multivariate System program version 2.1-pc. Results showed that the 60 genotypes demonstrated normal distribution in both locations for days to R1 (32-48d), days to R3 (35-55d), days to R7 (75-92d), and days to R8 (78-99d). Four early maturing genotypes and three late genotypes were obtained. Total SSR alleles observed were 237 with average allele per locus of 12.6 (3-29), and average PIC value of 0.78 (0.55-0.89). Genetic similarity among genotypes ranges from 74.8-95%. At similarity level 77% divided the genotypes into six clusters (the four selected early maturing genotypes located in clusters III and IV, while the three late genotypes located in cluster II). Based on maturity data, pubescent color, and phygenetic analysis seven parents were selected (four early maturing genotypes B1430, B2973, B3611, B4433 and three late genotypes B1635, B1658, and B3570). Twelve F2 populations were developed with the aid of SSR markers Satt300 dan Satt516. Two of the populations will be used to develop DNA markers for earliness in soybean.

PENGARUH KEMITRAAN TERHADAP KEUNTUNGAN USAHA PENGGEMUKAN DOMBA DI KABUPATEN BOGOR

Forum Agribisnis Vol 4, No 1 (2014): FA Vol 4 No 1 Maret 2014
Publisher : Forum Agribisnis

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Abstract

Bojong Jengkol Village Ciampea Bogor is the production center of male sheep. To overcome thecapital, management, and marketing problem in male sheep fattening, having a partnership with CV. Mitra Tani Farm is one of the solution for the farmer, though not all of the farmers in the village participate in the partnership.This study aims to analyse the implementation of the partnership, to describe the farming performance with descriptive analysis, and to comparethe male sheep fatteningprofitof farmers who have a partnership with Mitra Tani Farm to that ofthose who do not. The results show that the non-partnership farmer obtainesthe highest ratio of profit to cash cost per SDD per period. On the other hand, in all scale, the ratio of profit to total cost per SDD per period is negative, which can be concluded that the fattening business is disadvantaging. However, the ratio of profit to total costof the partnership  farmers better than that of non-partnership farmer since they have the smallest loss.

Phylogenetic and Maturity Analyses of Sixty Soybean Genotypes Used for DNA Marker Development of Early Maturity Quantitative Trait Loci in Soybean

Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Abstract

Phylogenetic and Maturity Analyses of Sixty SoybeanGenotypes Used for DNA Marker Development of EarlyMaturity Quantitative Trait Loci in Soybean. I MadeTasma, Dani Satyawan, Ahmad Warsun, MuhamadYunus, and Budi Santosa. The Indonesian soybeanproductivity is still very low with the national average of 1.3t/ha. One means to improve national soybean productivity isby manipulating harvest index by cultivating very earlymaturing soybean cultivars. Development of early maturingsoybean cultivars can be expedited by using marker-aidedselection. The objective of this study was to select parentallines having contrasted maturity traits and selected parentsmust be genetically distance. The parents then were used todevelop F2 populations for detecting early maturity QTL insoybean. Maturity tests of 60 soybean genotypes wereconducted at two locations, Cikeumeuh (Bogor) and Pacet(Cianjur) using a randomized block design with threereplications. Genomic DNA of the 60 genotypes wereanalyzed using 18 SSR markers and genetic relationship wasconstructed using the Unweighted Pair-Group MethodArithmatic through Numerical Taxonomy and MultivariateSystem program version 2.1-pc. Results showed that the 60genotypes demonstrated normal distribution in bothlocations for days to R1 (32-48d), days to R3 (35-55d), days toR7 (75-92d), and days to R8 (78-99d). Four early maturinggenotypes and three late genotypes were obtained. TotalSSR alleles observed were 237 with average allele per locusof 12.6 (3-29), and average PIC value of 0.78 (0.55-0.89).Genetic similarity among genotypes ranges from 74.8-95%.At similarity level 77% divided the genotypes into six clusters(the four selected early maturing genotypes located inclusters III and IV, while the three late genotypes located incluster II). Based on maturity data, pubescent color, andphygenetic analysis seven parents were selected (four earlymaturing genotypes B1430, B2973, B3611, B4433 and threelate genotypes B1635, B1658, and B3570). Twelve F2populations were developed with the aid of SSR markersSatt300 dan Satt516. Two of the populations will be used todevelop DNA markers for earliness in soybean.

Pengaruh Perlakuan Quenching-Tempering Terhadap Kekuatan Impak Pada Baja Karbon Sedang

JURNAL TEKNIK MESIN Vol 2, No 1 (2016): Oktober
Publisher : JURNAL TEKNIK MESIN

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Abstract

Penelitian ini membahas tentang pengaruh perlakuan panas terhadap kekuatan impak logam baja karbon sedang. Proses perlakuan panas yang diterapkan pada penelitian ini yaitu proses quenching dan tempering, dimana pada proses quenching pemanasan terhadap spesimen dilakukan hingga mencapai temperatur austenit yaitu 910°C yang kemudian ditahan selama 30 menit. Setelah itu spesimen tersebut didinginkan secara cepat dengan menggunakan media oil quenching. Selanjutnya, spesimen dipanaskan kembali (tempering) hingga mencapai temperatur 300°C, 400°C, 500°C, 600°C. Yang kemudian pada masing-masing temperatur tempering tersebut ditahan selama 60 menit. Setelah itu spesimen tersebut didinginkan pada temperatur ruangan.       Pada spesimen-spesimen yang telah mengalami proses perlakuan panas tersebut dilakukan pengujian impak. Kekuatan impak rata-rata baja karbon yang tidak mengalami proses perlakuan panas adalah 0,166 J/mm2. Pada baja yang hanya mengalami proses quenching kekuatan impak rata-rata yang dimiliki adalah 0,142 J/mm2. Pada baja karbon sedang yang mengalami proses tempering 300°C adalah 0,422 J/mm2. Pada tempering 400°C adalah 0,525 J/mm2. Pada tempering 500°C adalah 0.604 J/mm2. Pada tempering 600°C adalah 1,249 J/mm2 dengan masing-masing waktu penahanan selama 60 menit. Dari hasil pengujian tersebut, baja karbon yang memiliki kekuatan impak tertinggi memiliki sifat mekanik ulet, sedangkan baja karbon yang memiliki kekuatan impak terrendah memiliki sifat mekanik getas.

Mapping of Resistance Genes to Brown Planthopper in Untup Rajab, an Indonesian Local Rice Variety

Jurnal AgroBiogen Vol 14, No 2 (2018): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Abstract

Brown planthopper (BPH) is a major rice pest in Indonesia. The most economical and effective approach to control the insect pest is by using resistant varieties. Exploring for resistance genes is, therefore, a prerequisite for effective breeding program for BPH resistance. This study aimed to map BPH resistance genes in Untup Rajab, an Indonesian local rice variety. Genetic map was constructed using an F2 population from a cross between TN-1 and Untup Rajab, and SNP markers from RiceLD SNP Chip. Phenotyping was performed using bulk seedling test on F2:3 seedlings against two BPH populations, i.e. X1 and S1. Four QTLswere identified on chromosomes 5, 6, 8, and 11 with PVE values of 7.63%, 9.40%, 17.66%, and 3.05%, respectively. Relatively normal distribution of resistance phenotype and the relatively low PVE values indicate that Untup Rajab has a quantitative resistance to BPH with two different resistance loci identified for each BPH test population. The QTL on chromosome 8 overlaps with OsHI-LOX gene, which is associated with resistance to BPH, and adjacent to another QTL for resistance to green leafhopper. The QTL on chromosome 6 was found near OsPLDα4 and OsPLDα5 genes which are related to BPH resistance. Meanwhile, the QTL intervals on chromosome 5 and 11 did not overlap with any known BPH QTLs or genes, which make them attractive candidates for novel BPH resistance gene discovery.