ROSSA YUNITA
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111, Indonesia Telp. (0251) 8337975

Published : 11 Documents
Articles

Found 11 Documents
Search

Evaluation and Selection of Mutative Artemisia (Artemisia annua L.) According to the Altitude Variants ENDANG GATI LESTARI, ENDANG GATI LESTARI ENDANG GATI LESTARI; SYUKUR, MUHAMAD; PURNAMANINGSIH, RAGAPADMI; YUNITA, ROSSA; FIRDAUS, ROHIM
HAYATI Journal of Biosciences Vol 18, No 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (46.74 KB)

Abstract

Induction of genetic variant of Artemisia annua L. was conducted through the application of gamma ray irradiation in 2007-2008. The aim was to obtain a plant with high artemisine content > 0.5% and late flowering period of about > 7 month after planting. Tweleve selected genotypes were subsequently examined to gain genetic stability on altitude of 1500, 950, and 540 m asl. The results showed that the plants had shorter flowering age in Cicurug (540 m asl) than that of  in Pacet (950 m asl) and Gunung Putri (1540 m asl). Genotype 8 had the latest age of flowering in the three locations than the other genotypes, however, the growth and biomass were the lowest. Vegetative growth of Artemisia in Pacet and Gunung Putri was better than those in Cicurug. Genotype of 15 in Cicurug and 5A genotype in Gunung Putri and Pacet had higher wet and dry weight than that of two other associates. Based on plant biomass, 5 genotypes from Gunung Putri and Pacet i.e. 1D, 3, 5A, 14, and 15 genotypes were selected, as well as 5 genotypes i.e. 1D, 3, 4, 5A, and 15 genotypes from Cicurug. Analisys on artemisin content successfully obtained 5 selected somaclone lines i.e. 1B, 2, 4, 14, and 3 somaclones.
MULTIPLIKASI TUNAS MELINJO (GNETUM GNEMON) SECARA INVITRO Yunita, Rossa
Jurnal Sagu Vol 3, No 01 (2004)
Publisher : Jurnal Sagu

Show Abstract | Original Source | Check in Google Scholar | Full PDF (2752.879 KB)

Abstract

An experiment has been done at Laboratorium TLs.suc Culture of "Balai Penelitian Bioteknologi danGenctik Pertanian", Bogor, starting on July 2001 until June 2(M)2. The objective of the research is to studymultiplication of melinjo bud (Gnetum gnemon) by using in vitro technique. The experiment used factorialcomplete randomized design witli 19 replications. The thidiazuron were used as treatments at the concentra-tion of 0; 0,1 and 03 Ppm in combination with three kinds of medium i.e. MS, WPM and Anderson. The resultshowed that treatment of melinjo bud thidiazuron 0,3 ppra in MS medium could increase number of budmultiplication.
REGENERASI TUNAS DARI KALUS YANG TELAH DIBERI PERLAKUAN IRADIASI PADA PADI VARIETAS FATMAWATI [Shoot Regeneration of the Fatmawati Rice Variant Radiated Callie] Yunita, Rossa; Lestari, Endang G; Dewi, Iswari S
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Original Source | Check in Google Scholar | Full PDF (154.311 KB)

Abstract

Gamma ray mutative induction for increasing genetic variation has been applied for plant prime variety engineering. The materials are derrived from seed organ, shoot and calli. Calli is a group of actively dividing cell and have not been organized to form plant. The benefit of using calli explant is that the gamma ray could directly shot to DNA in the nuclear cell in such a way that there is higher opportunity for genetic change to occur. The problema of using calli explant are the difficulties in regenerating the calli into shoots, due to the deformation as a result of radiation process. Therefore, this research is aimed at obtaining the appropriate media formulation for shoot regeneration from Fatmawati-rice calli which has been irradiated with gamma ray. The reseach was conducted in BB-Biogen laboratory consisting of three experiments, such as : (1) calli iradiation with the dosage of 0; 5; 10; 15; 20; 25; 30; 35; 40; 45; 50; 55 and 60 Gy, (2) shoot regeneration at the MS + BA (0, 1, dan 3 mg/l) + IAA (0 dan 0,8 mg/l) media, and BA (0, 1, and 3 mg/l) + zeatin ( 0; 0,1; 0,2 and 0,3 mg/l) + IAA 0,8 mg/l and (3) Shoot induction at MS + IBA (0, 1, 2 and 3 mg/l) media. The result shows that the range of LD50 was obtained at the dosage of 30 Gy, the most apropriate media for shoot regeneration is MS + BA 3 mg/l + IAA 0,8 + zeatin 0,1 mg/l and media for root induction is IBA 1 mg/l.
Pengaruh Iradiasi Gamma dan Ethyl Methan Sulfonate Terhadap Pembentukan Embriosomatik Kedelai (Glycine max L.) Purnamaningsih, Ragapadmi; Mariska, Ika; Lestari, Endang Gati; Hutami, Sri; Yunita, Rossa
Jurnal Aplikasi Isotop Radiasi Vol 10, No 1 (2014): Juni 2014
Publisher : BATAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (24.638 KB)

Abstract

Kedelai merupakan salah satu sumber protein dan lemak nabati yang penting. Perubahan iklim global berpengaruh terhadap produktivitas kedelai, sehingga diperlukan kultivar-kultivar baru yang mempunyai sifatunggul tertentu agar produktivitas kedelai dapat ditingkatkan. Teknik in vitro dengan mutasi dan keragaman somaklonal merupakan meoda alternatif untuk memperoleh varietas baru apabila material genetik sebagai bahan seleksi tidak tersedia. Induksi mutasi dapat dilakukanpada populasi sel embriogenik dengan menggunakan iradiasi sinar gamma atau senyawa kimia, antara lain Ethyl Methan Sulfonate (EMS). Kedua metoda tersebut telah banyak digunakan untuk meningkatkan keragaman genetik tanaman dan telah dihasilkan galur-galurbaru dengan sifat unggul. Salah satu masalah penting yang harus dikuasai dalam penerapan teknologi tersebut adalah meregenerasikan sel somatik hasil mutasi dan keragaman somaklonal agar dapat ditumbuhkan menjadi planlet (tunas in vitro). Beberapa faktor yangmempengaruhi regenerasi tanaman adalah jenis bahan tanaman, genotipe, komposisi media, dll. Perlakuan keragaman somaklonal dan mutasi yang diberikan dapat menyebabkan kerusakan pada sel sehingga diperlukan modifikasi pada metoda regenerasi yang sudah diketahui agar populasi sel yang hidup setelah perlakuan mutasi dapat tumbuh menjadi tunastunas mutan. Tujuan penelitian adalah untuk mendapatkan planlet mutan hasil perlakuan mutasi dengan iradiasi gamma dan EMS. Varietas kedelai yang digunakan adalah Wilis, Burangrang, Baluran dan aksesi No. B 3592. Eksplan yang digunakan adalah embriozigotik muda berasal dari polong yang berumur 12-20 hari setelah penyerbukan. Induksi kalusembriogenik dilakukan dengan menggunakan media MS + vitamin Gamborg (B5) dengan penambahan 2,4-D 20 mg/l dan sukrosa 3%. Kalus yang didapatkan diberi perlakuan mutasi menggunakan sinar gamma pada dosis 400 rad atau direndam dalam larutan EMS (0.1 %, 0.3%, dan 0.5 %) selama 1, 2 dan 3 jam. Selanjutnya kalus dipindahkan pada media untuk menginduksi pembentukan benih somatik. Hasil penelitian menunjukkan bahwa pembentukan kalus dipengaruhi oleh genotipe tanaman. Pembentukan kalus tertinggi dihasilkan dari Baluran (93.40%) dan terendah Burangrang (75.90%). Perlakuan iradiasigamma menurunkan pembentukan struktur torpedo, dimana struktur torpedo tertinggi diperoleh dari Burangrang (25.4-26.3/eksplan). Aksesi B 3592 mempunyai kemampuan membentuk struktur torpedo paling tinggi pada semua perlakuan EMS yang digunakan. Perendaman kalus dalam larutan EMS 0.5% selama 1, 2, dan 3 jam menurunkan regenerasinya membentuk struktur torpedo pada semua genotipe. Perlakuan EMSmenyebabkan kerusakan sel yang lebih besar dibandingkan dengan iradiasi sinar gamma, ditunjukkan dengan persentase pembentukan struktur torpedo setelah perlakuan EMS (0-15/eksplan) lebih kecil dibanding dengan iradiasi sinar gamma (10.3-26.3/eksplan).Kata kunci : Glycine max, iradiasi sinar gamma, Ethyl Methan Sulfonate, embriogenesis somatik  ABSTRACTThe Effect of Gamma Irradiation and Ethyl Methan Sulfonate on Somatic Embryo Formation of Soybean (Glycine max L.). Soybean is a source of protein and vegetable oil. Global climate change affect the productivity of soybean, so that new cultivars that have superior characteristic can be produced. In vitro techniques through somaclonal variation andmutation is one alternative for obtaining new varieties when genetic material as the material selection is not available. Mutation induction can be performed on embryogenic cell populations using gamma irradiation or chemical compounds, such as Ethyl Methane Sulfonate (EMS). Both of these methods have been widely used to increase the geneticdiversity of plants and have produced new clones with superior characteristic. The main component that must be controlled in the implementation of these technologies is somatic cells regeneration after mutation treatment in order to get in vitro shoots. Regenerationmethods which are successfully applied to certain varieties, often is not successfully for other varieties of the same species. Some factors that influence it, are such as explants source, genotype, medium composition, genotype, medium composition, etc. Somaclonal variation and mutation treatment can cause cell damage that is sometimes necessary need modifications of the regeneration method that has been produced before. The aim of the experiment was to get cell population and planlet mutation with gamma iradiadion and Ethyl Methan Sulfonate (EMS). Young embryozygotic was used as explant came from young pod that was harvested at 12-20 days after fertilization of Wilis, Burangrang and Baluran varieties and accession No B 3592. Embryogenic callus induction was done by using MS media with vitamin B5 added with 20 mg/l of 2,4-D and 3% sucrose. The callus were irradiated by gamma rays 400 rad or dilutein EMS solution with 0.1%, 0.3% and 0.5% concentration for 1, 2, and 3 hours. After mutation treatment, the callus were sub culture for seed somatic induction. The results showed that callus formation was influenced by plant genotype. All genotipe were able produced callus, where the highest percentage callus production was Baluran (93.40 %) and the lowest of that was Burangrang (75.90 %). Gamma irradiation reduces formation of torpedo structure. The highest torpedo structure after gamma irradiation was obtained from Burangrang (25.4-26.3/eksplan). Accession B 3592 had the ability to form torpedo structure highest among all treatments EMS used. Callus immersion in a solution of 0.5% EMS for 1, 2, and 3 hoursdecreased callus regeneration to formed torpedo structure in all  genotypes. EMS treatment causes greater cell damage than the gamma-ray, indicated by the percentage of the torpedo structure formation after EMS treatment (0-15/eksplan) which was smaller than the gammaray irradiation (10.3-26.3/explant). Key words : Glycine max, irradiation gamma rays, Ethyl Methan Sulfonate, somatic embryogenesis
KOMUNIKASI PENDEK Perbanyakan Tanaman Artemisia annua secara In Vitro Yunita, Rossa; Lestari, Endang G.
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Original Source | Check in Google Scholar | Full PDF (174.426 KB)

Abstract

Artemisinin, an anti-malarial medicineisolated from the annual wormwood Artemisia annua,has a marked activity against chloroquine-resistant andchloroquine-sensitive strains of Plasmodium falciparum.This compound is useful for treatment of cerebral malaria.An in vitro propagation system for A. annua has been developed.Shoots were induced by culturing seeds of A.annua on a MS medium containing BAP (0, 0.1, 0.3, 0.5mg/l). Shoots were also formed on each seedling culturedon the same medium. Root formations were obtained fromshoots that were subcultured on a MS medium containingIBA (0, 1.0, 1.5, 2 mg/l). The results showed that MS mediumsupplemented with BAP 0.3 mg/l was the best medium forinduction and multiplication of the shoots, while the MSmedium supplemented with IBA (1 mg/l) was good for rootformations.
Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro Ariyanti, Febrina; Tumilisar, Christiani; Yunita, Rossa
Bioma Vol 10 No 1 (2014): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Original Source | Check in Google Scholar | Full PDF (714.873 KB)

Abstract

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin
INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) Yunita, Rossa; Lestari, Endang Gati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Original Source | Check in Google Scholar | Full PDF (856.858 KB)

Abstract

In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction.
Pengaruh Iradiasi Gamma dan Ethyl Methan Sulfonate Terhadap Pembentukan Embriosomatik Kedelai (Glycine max L.) Purnamaningsih, Ragapadmi; Mariska, Ika; Lestari, E.G.; Hutami, Sri; Yunita, Rossa
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 10, No 1 (2014): Juni 2014
Publisher : BATAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (170.167 KB)

Abstract

Kedelai merupakan salah satu sumber protein dan lemak nabati yang penting. Perubahan iklim global berpengaruh terhadap produktivitas kedelai, sehingga diperlukan kultivar-kultivar baru yang mempunyai sifat unggul tertentu agar produktivitas kedelai dapat ditingkatkan. Teknik in vitro dengan mutasi dan keragaman somaklonal merupakan meoda alternatif untuk memperoleh varietas baru apabila material genetik sebagai bahan seleksi tidak tersedia. Induksi mutasi dapat dilakukan pada populasi sel embriogenik dengan menggunakan iradiasi sinar gamma atau senyawa kimia, antara lain Ethyl Methan Sulfonate (EMS). Kedua metoda tersebut telah banyak digunakan untuk meningkatkan keragaman genetik tanaman dan telah dihasilkan galur-galur baru dengan sifat unggul. Salah satu masalah penting yang harus dikuasai dalam penerapan teknologi tersebut adalah meregenerasikan sel somatik hasil mutasi dan keragaman somaklonal agar dapat ditumbuhkan menjadi planlet (tunas in vitro). Beberapa faktor yang mempengaruhi regenerasi tanaman adalah jenis bahan tanaman, genotipe, komposisi media, dll. Perlakuan keragaman somaklonal dan mutasi yang diberikan dapat menyebabkan kerusakan pada sel sehingga diperlukan modifikasi pada metoda regenerasi yang sudah diketahui agar populasi sel yang hidup setelah perlakuan mutasi dapat tumbuh menjadi tunas-tunas mutan. Tujuan penelitian adalah untuk mendapatkan planlet mutan hasil perlakuan mutasi dengan iradiasi gamma dan EMS. Varietas kedelai yang digunakan adalah Wilis, Burangrang, Baluran dan aksesi No. B 3592. Eksplan yang digunakan adalah embriozigotik muda berasal dari polong yang berumur 12-20 hari setelah penyerbukan. Induksi kalus embriogenik dilakukan dengan menggunakan media MS + vitamin Gamborg (B5) dengan penambahan 2,4-D 20 mg/l dan sukrosa 3%. Kalus yang didapatkan diberi perlakuan mutasi menggunakan sinar gamma pada dosis 400 rad atau direndam dalam larutan EMS (0.1 %, 0.3 %, dan 0.5 %) selama 1, 2 dan 3 jam. Selanjutnya kalus dipindahkan pada media untuk menginduksi pembentukan benih somatik. Hasil penelitian menunjukkan bahwa pembentukan kalus dipengaruhi oleh genotipe tanaman. Pembentukan kalus tertinggi dihasilkan dari Baluran (93.40%) dan terendah Burangrang (75.90%). Perlakuan iradiasi gamma menurunkan pembentukan struktur torpedo, dimana struktur torpedo tertinggi diperoleh dari Burangrang (25.4-26.3/eksplan). Aksesi B 3592 mempunyai kemampuan membentuk struktur torpedo paling tinggi pada semua perlakuan EMS yang digunakan. Perendaman kalus dalam larutan EMS 0.5% selama 1, 2, dan 3 jam menurunkan regenerasinya membentuk struktur torpedo pada semua genotipe. Perlakuan EMS menyebabkan kerusakan sel yang lebih besar dibandingkan dengan iradiasi sinar gamma, ditunjukkan dengan persentase pembentukan struktur torpedo setelah perlakuan EMS (0-15/eksplan) lebih kecil dibanding dengan iradiasi sinar gamma (10.3-26.3/eksplan). Kata kunci :   Glycine max, iradiasi sinar gamma, Ethyl Methan Sulfonate, embriogenesis somatik
INDUKSI MUTASI DENGAN KOLKISIN DAN SELEKSI IN VITRO TEBU TOLERAN KEKERINGAN MENGGUNAKAN POLYETHYLENE GLYCOL / Induced Mutation using Colchicine and In vitro Selection using Polyethylene glycol for Drought-Tolerant Sugarcane Hartati, RR. Sri; Suhesti, Sri; Yunita, Rossa; Syafaruddin, Syafaruddin
Jurnal Penelitian Tanaman Industri Vol 24, No 2 (2018): Desember, 2018
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Original Source | Check in Google Scholar | Full PDF (19933.079 KB)

Abstract

Creating of varieties can be done through mutation breeding at the cellular level, combined with in vitro selection. This research was conducted at the UPBUP from January until December 2017 to find out colchicine concentration and treatment duration which effectively produced tolerant mutant through in vitro drought selection using polyethylene glycol (PEG). The study consists of two stages. The first was mutation induction on sugarcane calli using colchicine, which was arranged factorially with a completely randomized environment design. The first factor was varieties (BL, PS 862, and PSJT 941), the second was colchicine concentration (0,01,0,0 and 0,05%), and the third was colchicine duration treatment (1 and 3 days). Observations were made on the percentage of callus survival. The second stage was in vitro selection of droughts using a PEG 6000, which was arranged factorially with a complete randomized design. The first factor was the concentration of colchicine (0, 0.01, 0.03, and 0.05%), the second was the colchicine duration treatment (1 and 3 days), and the third was PEG concentration (0, 10 and 20%). Selection was done for 4 weeks. Percentage of live callus, regenerated callus, number and height of shoots were observed as a selected criteria. Colchicine treatment in the 0.01 - 0.05% for 3 days on PS 862 and 0.01 - 0.03% for 3 days on PSJT 941 callus resulted mutant passing in vitro drought selection at 10% PEG concentration level. Mutant selection will be continued through in vivo. The optimum mutation treatment for BL has not been obtained.Keywords: chemical mutagen, colchicine, mutation, selection agent, PEG 6000 AbstrakPerakitan varietas tebu toleran kekeringan dapat dilakukan melalui pemuliaan mutasi pada tingkat sel, dikombinasikan dengan seleksi in vitro. Penelitian dilakukan di Laboratorium Unit Pengelola Benih Unggul Pertanian (UPBUP), Bogor, mulai Januari sampai Desember 2017 dengan tujuan mengetahui konsentrasi dan lama perlakuan mutagen kimia kolkisin, yang dapat menghasilkan mutan tebu yang lolos seleksi kekeringan secara in vitro menggunakan agen penyeleksi polyethylen glycol (PEG). Penelitian terdiri dari 2 tahap. Tahap pertama adalah induksi mutasi pada kalus tebu menggunakan mutagen kimia kolkisin. Penelitian disusun secara faktorial dengan rancangan lingkungan Acak Lengkap. Faktor pertama varietas tebu (BL, PS 862, dan PSJT 941), faktor kedua konsentrasi kolkisin (0, 0,01, 0,03, dan 0,05%), dan faktor ketiga lama perlakuan kolkisin (1 dan 3 hari). Pengamatan dilakukan terhadap persentase kalus hidup. Tahap kedua adalah seleksi kekeringan secara in vitro menggunakan PEG 6000. Penelitian disusun secara faktorial dengan rancangan Acak Lengkap. Faktor pertama konsentrasi kolkisin (0; 0,01; 0,03; dan 0,05%), faktor kedua lama perlakuan kolkisin (1 dan 3 hari), dan faktor ketiga konsentrasi PEG (0; 10; dan 20%). Seleksi dilakukan selama 4 minggu. Persentase kalus hidup, kalus yang berregerenerasi, jumlah dan tinggi tunas, diamati sebagai kriteria kalus mutan lolos seleksi. Perlakuan kolkisin pada kisaran konsentrasi 0,01 – 0,05% selama 3 hari pada kalus PS 862 dan 0,01 – 0,03% selama 3 hari pada PSJT 941 dapat menginduksi kalus mutan yang lolos seleksi kekeringan in vitro pada tingkat konsentrasi PEG 10%. Seleksi mutan akan dilanjutkan secara in vivo. Perlakuan mutasi yang optimum untuk BL belum diperoleh.Kata kunci: mutagen kimia, kolkisin, mutasi, agen penyeleksi, PEG 6000
PEMBENTUKAN PLANLET MUTAN TEBU TOLERAN NATRIUM KLORIDA DENGAN MUTASI DAN SELEKSI IN VITRO / Mutant Plantet Formation Of Sugarcane Tolerant Sodium Chloride With In Vitro Selection and Mutation Yunita, Rossa; Hartati, RR. Sri; Suhesti, Sri; Syafaruddin, Syafaruddin
Jurnal Penelitian Tanaman Industri Vol 25, No 1 (2019): Juni, 2019
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Original Source | Check in Google Scholar | Full PDF (507.223 KB)

Abstract

The needs of sugarcane continue to increase is one the solution demand. However, the available land is sub-optimal land such as saline land. For this reason, salinity tolerant varieties are needed. To assemble sugarcane varieties that are tolerant of salinity stress can use the induction mutation technology using gamma rays combined with in vitro selection in vitro selection using NaCl. The purpose of this study was to obtain sugarcane mutant planlets which were tolerant to the salinity results of induction mutations and in vitro selection. The plant material used in this study were PS862 and PSJT941 sugarcane varieties. The environmental design used in this study was a completely randomized design. This study consisted of four main stages of activity namely (1) mutation induction using gamma ray irradiation (5, 10, 15 20, 25.30 and 35 Gy) and in vitro selection on media containing NaCl; (2) bud regeneration in MS medium + BA 3 mg / l + Zeatin 0.3 mg / l + Proline 100 mg / l for sugarcane callus PS862 and MS varieties + BA 3 mg / l + Zeatin 0.1 mg / l + Proline 100 mg / l for sugarcane callus PSJT941 and (3) root induction on MS + IBA 1 mg / l. The results of this study were 122 mutant plantlets originating from the PS862 variety and 66 mutant planlets originating from PSJT941 which were tolerant to NaCl salt stress. The mutants obtained were salinity tolerant because they were able to grow on media containing NaCl. To produce a population that is salinity tolerant, it is necessary to test it in a greenhouse and in the field that is gripped by salinity.Keywords : Salt stress, iradiation, PS862, PSJT941, Saccharum sp AbstrakKebutuhan komoditas tebu terus meningkat, untuk memenuhinya dapat dilakukan dengan cara ekstensifikasi. Namun demikian, lahan yang tersedia adalah lahan sub optimal seperti lahan salin, untuk itu diperlukan varietas toleran salinitas.  Untuk merakit varietas tebu yang toleran terhadap cekaman salinitas dapat mengunakan teknologi mutasi induksidengan menggunakan sinar gamma yang dikombinasikan dengan seleksi in vitro, Sedangkan untuk menyeleksi kalus secara in vitro digunakan NaCl. Tujuan penelitian ini adalah untuk mendapatkan   planlet-planlet mutan tebu toleran salinitas hasil mutasi induksi dan seleksi in vitro. Bahan tanaman yang digunakan pada penelitian ini adalah kalus tebu varietas PS862 dan PSJT941. Rancangan lingkungan yang digunakan pada penelitian ini adalah rancangan acak lengkap.  Penelitian ini yang  terdiri  atas empat tahap  kegiatan utama yaitu (1) induksi mutasi dengan menggunakan iradisi sinar gamma  (5, 10, 15 20, 25,30 dan 35 Gy) dan seleksi in vitro pada media yang mengandung NaCl;  (2) regenerasi tunas pada media MS + BA 3 mg/l + Zeatin 0,3 mg/l + Prolin 100 mg/l untuk kalus tebu varietas PS862  dan MS + BA 3 mg/l + Zeatin 0,1 mg/l + Prolin 100 mg/l untuk kalus tebu PSJT941dan (3) induksi akar pada media MS + IBA 1 mg/l. Hasil yang diperoleh dari penelitian ini adalah  122  planlet mutan  yang berasal dari varietas PS862 dan 66 planlet mutan yang berasal dari PSJT941 yang toleran cekaman garam NaCl. Mutan yang diperoleh memiliki sifat toleran salinitas karena mampu tumbuh pada media yang mengadung NaCl.  Untuk menghasilkan populasi yang toleran salinitas perlu dilakukan pengujian di rumah kaca dan dilapang yang tercekam salinitas.Kata Kunci :  Cekaman garam, iradiasi, PS862, PSJT941, Saccharum sp