Gusti Ayu Yuniati Kencana
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SEROPREVALENSI INFEKSI VIRUS NEWCASTLE DISEASE DAN DETEKSI PARAMYXOVIRUS PADA ITIK DI PETERNAKAN DAN PASAR UNGGAS DI BALI Purwanda, I GBA; Mahardika, I Gusti Ngurah Kade; Yuniati Kencana, Gusti Ayu
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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Seroprevalence of Newcastle Disease Virus (NDV) infection and the presence of paramyxovirus on ducksat the farmland and the poultry market in Bali have not been known. The purpose of this study was todetermine the comparison of seroprevalence of NDV infection and the presence of paramyxovirus on duckat farmlands and poultry markets in Bali. Locations sampled were Gelgel, Tojan, Akah, Takmung, Tusanvillages in Klungkung Regency, as well as Mengwi, Mengwitani, Lukluk, Sangeh, and Blahkiuh villages ofBadung Regency. The poultry market samples were Galiran of Klungkung Regency, and Bringkit ofBadung Regency. Serum samples and cloacal-tracheal swabs were taken using stratified-random samplingfrom adult ducks of both markets and farmland that had  more than 500 individuals in a flock. Samplingwas carried out every month for 6 months. Antibody against NDV was detected with InhibitionHaemagglutination test (HI). Tracheal and cloacal swabs were propagated in fertile chicken eggs of 9-11days old. Paramyxovirus was detected by the haemagglutination (HA) test and Reverse TranscriptasePolymeraseChain Reaction (RT-PCR). The correlation between NDV seroprevalences at farmland andpoultry markets was analyzed using non-parametric test of Chi-square. The results showed that theseroprevalence of NDV on March until August 2012  reached 45% on farmlands in both regencies, while inthe markets were up to 32.6%. There was no correlation between NDV seroprevalence at farmlands andpoultry markets in the two regencies (r = 0.522, P> 0.05). The paramyxoviruses detected were APMV-5and APMV-8, while NDV was not found.
AMINO-TERMINUS OF POLYMERASE BASIC-2 OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM VARIOUS ANIMAL SPECIES IN INDONESIA Yuniati Kencana, Gusti Ayu; Asmara, Widya; Rangga Tabbu, Charles; Kade Mahardika, I Gusti Ngurah
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The information on pathogenicity and adaptation factors of avian influenza virus (AIV) in mammalsis very inportant in an effort to reduce the risk of avian influenza (AI) pandemic in the future. Polymerasegene complex appears to be the major factors for adaptation of AIV to certain animal species. A preliminarystudy on role of non-coding region (NCR) and amino-terminus of polymerase-basic 2 (PB2) is presented.Purified viral RNA of AIV isolated from chicken, duck, pig, and quail of Bali and Yogyakarta was reversetranscribed into cDNA and amplified using reverse transcriptase-polymerase chain reaction (RT-PCR)using PB-2 universal forward primer and specifically designed backward primer. The result showed thatall AIV?s H5N1 isolated from chicken, duck, quail, and pig, posed PB2 amino-terminus typical for IndonesianAIV H5N1. However, polymorphic amino acids of the protein fragment did not show any species specificmotive, with the exception of the pig isolate Sw/Tabanan/2006 which had specific substitution of D16E,H17Q, M40I, and H124Y.
THE STABILITY OF THE BALI’S RABIES VIRUS MOLECULAR MARKER FOR THE DEVELOPMENT OF DIAGNOSTIC METHOD Dimas Abiyoga, Putu; Yuniati Kencana, Gusti Ayu; Dibia, I Nyoman
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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The objectives of this study were to prove that the molecular marker of Bali?s rabies virus is still conserveand to develop a diagnostic method based on molecular marker. Thirty brain samples of dog that had beeninfected by a rabies virus from 2014 and 2015 were used for this research. The sequences of nucleotidewhich were obtained and the sequences of nucleotide accessed in GenBank were analyzed using  MEGA5.2 software. The result provided that the specific amino acid (isoleusin) at position 308 (open readingframe) as a molecular marker of Bali?s rabies virus was still conserve. Fragment of N gene amplified byReverse RT-PCR method with a specifically designed primers showed that every isolate of rabies virus hadit?s own typical band, and could distinguish Bali isolate from the others in Indonesia.