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Efek Kombinasi Ekstrak Daun Jati Belanda (Guazuma ulmifolia Lamk.) Dan Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) Terhadap Penurunan Kadar Glukosa Darah Dan Histopatologi Pankreas Mencit Diabetes Induksi Aloksan Yuliet, Yuliet
e-Journal Natural Science FMIPA UNTAD Vol 1, No 1 (2012)
Publisher : e-Journal Natural Science FMIPA UNTAD

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Abstract

Evaluation towards the effect of antidiabetic of combination water extract of mutamba leaves (Guazuma ulmifolia Lamk.) and ethanolic extract of temulawak was conducted alloxan induced diabetic mice. Effect of extracts on blood glucose levels of all mice was determined at various time interval at 1, 3, 7, 14, 21, and 28 days. On the first day after treatment in alloxan diabetic mice and the 28th days later, histopathologic examination and grading were carried out on Gomori stained and the number of β cells in islet of Langerhans of pancreas was counted to observe the regeneration of the pancreatic β cells. The test materials used were combination of water extract of mutamba leaves (JB) with the dose of 25 m/kg bw and ethanolic extract of temulawak rhizomes (TL) with the dose of 25 mg/kg bw; JB 50 & TL 50; JB 50 & TL 25; JB 25 & TL 12.5, with metformin and glibenclamide as references at the doses of 195 mg/kg bw and 0.65 mg/kg bw, respectively. In the alloxan induced method, giving the tested material in combination dose for 28 days decreased the blood glucose level in alloxan induced diabetic mice. The percentage of the blood glucose level difference increased up to the day 28 compared to the control group. The decrease of the blood glucose level significantly different compared to the control group was seen at the day 14, 21 and 28. Based on the analysis pancreas histopathology and the counting number of the β cells in islet of Langerhans of pancreas, it was shown that the combination of JB 25 & TL 12.5 gave the better antidiabetic activity and significantly different with the control group (p<0.05) compared to the other treatment.
FORMULASI KRIM ANTIOKSIDAN EKSTRAK ETANOL BAWANG HUTAN (ELEUTHERINE PALMIFOLIA L. MERR) Sharon, Nela; Anam, Syariful; Yuliet, Yuliet
Online Journal of Natural Science FMIPA Vol 2, No 3 (2013): Volume 2 Number 3 (December 2013)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Eleutherine palmifolia L. Merr having antioxidant activity, its because of contain flavonoid, fenolat and tanin. These compounds have effect in  preventing of free radical which cause premature aging. Research on ethanol extract of E. palmifolia L. Merr which formulated into antioxidant cream have been done by applying variation of TEA emulgator and stearic acid. That variation  were F1 (2% : 6%), F2 (3% : 12%) and F3 (4% : 18%). Determination of antioxidant activity  was done based on DPPH methode, and the Value is 98,210%. Furthermore, evaluation on  physical characteristics was done based on organoleptic test, homogenity, pH, viscosity and stability of cream preparation. Observation was done during 21 days. Obtained data were then analyze by student-t test in comparing of the value between fresh and 21 days after storage. the pH of cream F1, F2 and F3 were not showed significant decrease, while DPPH reduce percent of F1, F2 and F3 were showed significant decrease.  In contrast, viscosity of cream F3 was significant decrease. Antioxydant activity extract based on DPPH reduce percent value of F1, F2, F3 at the first day were 96,822%, 97,123% and 96,896%, respectively. Whereas that value  at 21nd days after storage  were 89,036%, 89,726% and 90,564%, respectively.  Our result showed that E. palmifolia L. Merr ethanol extract  can be formulated as a cream preparations. F2 physical properties was  fullfill standart quality, which it was used TEA emulgator and stearic acid proportion of 3% : 12%.  
Kajian Aktivitas Antioksidan Ekstrak Air dan Minyak Buah Tomat (Solanum lycopersicum L.) Yuliet, Yuliet
Media Eksakta Vol 7, No 1 (2011)
Publisher : Media Eksakta

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Abstract

Tomato (Solanum lycopersicum L.) represent an edible fruit which is easily available and known as a source of antioxidant. This research was aimed to test the antioxidant activity of tomato extract in olive oil and water. Antioxidant activity was determined using superoxide dismutase (SOD) and lipid peroxidation (LPO) activity test, ex vivo in Swiss Webster male mice. Result showed that olive oil tomato extract increased significantly (p<0.01) superoxide dismutase (SOD) activity and inhibited lipid peroxidation. The highest activity against superoxide dismutase (SOD) activity (0.85%) and inhibited lipid peroxidation in liver (74.67 %) was given by tomato extract in olive oil at 0.075 mL/kg bw (p<0.01). The highest inhibition of lipid peroxidation in kidney was given by tomato extract in water at dose of 150 mg/kg bw (20.27 %, p<0.01).
Uji Aktivitas Antiinflamasi Gel Ekstrak Buah Kaktus (Opuntia elatior Mill.) Pada Tikus (Rattus norvegicus L.) Yang Diinduksi Lamda Karagenan Sativa, Oryza; Yuliet, Yuliet; Sulastri, Evi
Online Journal of Natural Science FMIPA Vol 3, No 2 (2014): Volume 3 Number 2 (August 2014)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

This research is about anti inflammatory activity of cactus fruits (Opuntia elatior Mill.) extract gel in rats (Rattus norvegicus L.) at induced lamda carragenan in purpose to determine anti inflammatory activity, and concentration of cactus fruits extract in the gel that have an anti inflammatory activity which is equal to sodium diclofenac 1% activity, and to determine the effect of variations in the concentration of cactus fruit extract (Opuntia elatior Mill) on the physical stability of the gel. Extract was made by remaseration method using 70% ethanol. Physical stability test was conducted based on organoleptic, pH, viscosity and homogeneity of the gel. Test on Antiinflammatory activity were divided into 5 groups of treatment. Each rats induced by using lambda carrageenan. The first group of rats was given a gel without active ingredient as a negative control, groups of 2nd, 3rd and 4th rats were given gel cactus fruit extract in concentration of 5%, 10% and 15 % respectively, and 5th groups of rats were given diclofenac sodium gel as a positive control. Diameter and volume of inflammation were measured during 6 hours with 30 minute interval. Colected data were statistically analyzed using ANOVA. The results showed that concentration of cactus fruit extract in proportion of 5%, 10% and 15% in the gel preparation showed different conditions on organoleptic observations and homogeneity even though there was no different in the pH and viscosity during 14 days of storage. The results on anti-inflammatory activity testusing concentration of 5%, 10% and 15% have a recuperation effect on inflammation Gel contining 10% of extract has anti inflammatory activity which equal to 1% of sodium diclofenac activityi.e. the percentage of diameter and volume of  inflammation were 63,75% and 20,93%.
FORMULASI TABLET EFFERVESCENT JAHE (Z Officinale Roscoe) DENGAN VARIASI KONSENTRASI SUMBER ASAM DAN BASA Kholidah, Sitti; Yuliet, Yuliet; Khumaidi, Akhmad
Online Journal of Natural Science FMIPA Vol 3, No 3 (2014): Volume 3 Number 3 (December 2014)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Penelitian mengenai formulasi tablet effervescentsari jahe. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan variasi konsentrasi sumber asam dan basa terhadap mutu fisik tablet.  Metode yang digunakan yaitu metode granulasi basah. Empat variasi formula masing-masing dengan konsentrasi asam basa 50%, 60%, 65% dan 70%. Evaluasi mutu fisik granul meliputi ujikadar air, uji kandungan lembab, uji kompresibilitas, uji waktu alir dan sudut diam. Evaluasi tablet pada hari 1 dan 21 untuk mengetahui stabilitas mutu fisik tablet yang meliputi uji keseragaman bobot, uji kekerasan tablet, uji kerapuhan tablet dan uji waktu larut. Data yang diperoleh dibandingkan dengan Farmakope Indonesia dan literatur lain lalu dianalisis dengan uji oneway ANOVA dan uji t-student. Hasil yang diperoleh menunjukkan penggunaan variasi konsentrasi asam basa tidak berpengaruh terhadap stabilitas mutu fisik tablet yang meliputi keseragaman bobot, kekerasan, kerapuhan tetapi berpengaruh terhadap waktu larut  tablet effervescent jahe. Konsentrasi asam basa yang memenuhi mutu fisik tablet yang optimum yaitu formula 4 dengan konsentrasi asam basa 70% (asam sitrat 8,58%, asam tartrat 27,17% dan  natrium bikarbonat 34,45%).
FORMULASI TABLET ANTIOKSIDAN EKSTRAK BAWANG HUTAN (Eleutherine bulbosa Mill.Urb) Karmini, Karmini; Yuliet, Yuliet; Khumaidi, Akhmad
Online Journal of Natural Science FMIPA Vol 3, No 3 (2014): Volume 3 Number 3 (December 2014)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Telah dilakukan penelitian mengenai formulasi tablet antioksidan ekstrak bawang hutan (Eleutherine bulbosa mill.urb) dengan tujuan untuk mengetahui pengaruh penggunaan variasi konsentrasi ekstrak bawang hutan pada mutu fisik dan aktivitas antioksidan tablet, mengetahui konsentrasi ekstrak yang memenuhi mutu fisik yang paling optimum dan mengetahui aktivitas antioksidan (IC50) dari tiap formula. Ekstrak dibuat dengan metode maserasi menggunakan pelarut etanol 96%. Proses pembuatan tablet dengan metode granulasi basah. Dilakukan uji mutu fisik granul dan uji mutu fisik tablet. Uji aktivitas antioksidan dilakukan secara in-vitro menggunakan metode DPPH pada formula 1, formula 2, formula 3 dan kontrol negatif dengan penggunaan Vitamin C sebagai kontrol positif. Data hasil pengukuran dianalisis secara statistik menggunakan metode ANOVA. Hasil penelitian menunjukkan bahwa penggunaan variasi konsentrasi ekstrak bawang hutan dengan konsentrasi 3,57%, 7,14% dan 10,71% pada sediaan tablet memperoleh mutu fisik tablet yang baik pada penyimpanan selama ± 1 bulan. Hasil uji aktivitas antioksidan dengan IC50 yang diperoleh pada hari ke-1 dan hari ke-28 kontrol negatif, formula 1, formula 2, dan formula 3 berturut-turut adalah 2110 ppm dan 2210 ppm; 126,18 ppm dan 135,73 ppm; 91,00 ppm dan 95,45 ppm; 46,87 ppm dan 50,93 ppm; dibandingkan dengan IC50 Vitamin C sebesar 4,93 ppm. Formula 1 mempunyai mutu fisik tablet yang paling optimum. Aktivitas antioksidan dari tiap formula masuk dalam kategori dari sangat kuat sampai sedang.
EFEKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL DAUN SUMAMBU (Hyptis capitata Jacq.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus L.) YANG DIINDUKSI DENGAN KARAGENAN Audina, Mia; Yuliet, Yuliet; Khaerati, Khildah
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

One of the medical plants that can be used as an anti-inflamation treatment is sumambu leaf (Hyptis capitata Jacq.). Most of people in binangga village, Subdistric of central Parigi, Regency of Parigi Moutong, have been used this sumambu leaf for deducting inflammation, swelling, and painful. The aimed of this research is to know the effectiveness of the anti-inflamation of Sumambu leaf (Hyptis capitata Jacq.) towards white male mouse (Rattus novergicus L.) which has been given carrageenan induction. The extract was made by using a maseration method using 96% ethanol liquid. The test of the effectiveness of anti-inflamation was divided into your treatments which each treatment was consist of five mice which have been given negative control  0.5% CMC-Na suspension ;100 mg/KgBW, 200 mg/KgBW, 300 mg/KgBW of sumambu leaf extract. After 60 minutes, 0.1 ml carregeenan has been injected using subplantar method at the paw of the mice. The inflamation volume measured using  pletysmometer for 6 hours.  The data collection was statistically analyzed by ANOVA (Analysis of variance) one direction and continued to Duncan test. The results of this research  showed that the three doses of sumambu extract which are ES 100 mg/KgBW, 200 mg/KgBW, 300 mg/KgBWhave the effect of anti-inflamation. Moreover, it can be said that the result of this research showed that the dose that has a lower inflamation percent was ES 300 mg/KgBW. Keywords: Anti-inflammation, Hyptis capitata Jacq., Carrageenan, Inflamed Paw Volume.
Efek Akar Garcinia rostrata Hassk.ex Hook.f Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan (Mus musculus) Yang Diinduksi Aloksan Bahman, Devi Saputri; Yuliet, Yuliet; Ihwan, Ihwan
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Garcinia rostrata Hassk.ex Hook.f is a plant that empirically used for lowering blood glucose levels. This study aims to determine antidiabetic activity of the n-hexane, ethyl acetate and ethanol-water fractions of its root Garcinia rostrata Hassk.ex Hook.f extract as well as to compare their effects to Glibenclamide as the positive control in alloxan-induced mice. The mice divided into five groups. The first group was given Na.CMC 0.5% (as negative control) and the second one was given Glibenclamide 0.65 mg/kgBW (as positive control), while the other three was given of n-hexane, ethyl acetate and ethanol-water fractions, each at a dose of 130 mg/kgBW. Before given the treatment, the mice was induced by alloxan at a dose of 150 mg/kgBW, by intraperitonial route. At fourth day after induced, the mice its raising blood glucose level >200 mg/dL was given a treatment 25 days by oral route. The level of mice blood glucose was measured 4 times at day 4, 11, 18, and 25, the blood is taken from lateralis vein tail of mice.  Data obtained were statistically analyzed by ANOVA and continued by Duncan test. Based on those results, it can be concluded that the ethanol-water fraction at doses of 130 mg/kgBW has antidiabetic activity in hyperglycemic that is comparable to Glibenclamide. Whereas, ethyl acetate fraction, at dose of 130 mg/kgBW has greater activity in lowering blood glucose level than glibenclamide. Keywords: Garcinia rostrata Hassk.ex Hook.f,  Fraction,  n-hexane,  Ethyl acetate, Ethanol-water, Alloxan. 
In Vitro And in Vivo Antidiabetic Activity of Ethanol Extract and Fractions of Hibiscus surattensis L Leaves Yuliet, Yuliet; Sukandar, Elin Yulinah; Adnyana, I Ketut
Indonesian Journal of Pharmaceutical Science and Technology Suppl 1, No. 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.099 KB) | DOI: 10.15416/ijpst.v1i1.16120

Abstract

Hisbiscus surattensis L the local name “tamoenju” is utilized by ethnic Sumari (Central Sulawesi) as an antidiabetic herbal, but de nitive studies on ef cacy and safety are lacking. Therefore, this study was aimed to investigate the antidiabetic activity of ethanolic extract of the leaves and fractions using in vitro and in vivo models. Ethanol extract (EE) of the leaves of H. surattensis L was prepared by maceration. The ethanol extract was separated into three fractions by liquid/liquid extractions to yield n-hexane (HF), ethyl acetate (EAF) and water fraction (WF). Firstly, the extract and fractions were tested for its inhibitory activity on α-glucosidase in vitro. The results showed IC50 EE, HF and WF more than 5000 ppm while the IC50 fraction of EAF 3888.34 ppm. Then, oral glucose tolerance test (OGTT) of the extract/fractions were carried out in normal mice. On OGTT, the ethanol extract, EAF, and WF at doses of 150 and 300 mg/kg BW were able to inhibit increasing the level of glucose in the blood. The results showed that the extract and fraction of H. surattensis L leaves in vitro had no effect as α-glucosidase inhibitor but in vivo had the hypoglycemic effect especially on the fraction of ethyl acetate and the water fraction. Key words: α-glucosidase, antidiabetic, Hibiscus suratttensis, glucose tolerance.
Efek Akar Garcinia rostrata Hassk.ex Hook.f Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan (Mus musculus) Yang Diinduksi Aloksan Bahman, Devi Saputri; Yuliet, Yuliet; Ihwan, Ihwan
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.242 KB)

Abstract

Garcinia rostrata Hassk.ex Hook.f is a plant that empirically used for lowering blood glucose levels. This study was aimed to determine antidiabetic activity of the n-hexane, ethyl acetate and ethanol-water fractions of its root Grostrata Hassk.ex Hook.f extract as well as to compare their effects to Glibenclamide as the positive control in alloxan-induced mice. The mice divided into five groups. The first group was given Na.CMC 0.5% (as negative control) and the second one was given Glibenclamide 0.65 mg/kgBW (as positive control), while the other three was given of n-hexane, ethyl acetate and ethanol-water fractions, each at a dose of 130 mg/kgBW. Before given the treatment, the mice was induced by alloxan at a dose of 150 mg/kgBW, by intraperitonial route. At fourth day after induced, the mice its raising blood glucose level >200 mg/dL was given a treatment 25 days by oral route. The level of mice blood glucose was measured 4 times at day 4, 11, 18, and 25, the blood is taken from lateralis vein tail of mice.  The Data were analyzed by ANOVA and continued by Duncan test. Based on those results, it can be concluded that the ethanol-water fraction at doses of 130 mg/kgBW has antidiabetic activity in hyperglycemic that is comparable to Glibenclamide. Whereas, ethyl acetate fraction, at dose of 130 mg/kgBW has greater activity in lowering blood glucose level than glibenclamide.