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SPECIFIC PROTEIN EXPRESSION OF GOAT PREIMPLANTATION EMBRYO IN VITRO AS AN EARLY PREGNANCY SIGNAL Lanus Sumadiasa, I Wayan; Yuliani, Enny
Jurnal Veteriner Vol 9, No 2 (2008)
Publisher : Jurnal Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of the experiment was to investigate the intensity of signal secreted by embryos for their survival in the early pregnancy. Embryos at morulla and blastocyst stages generated by in vitro fertilization were frozen and then thawed for in vitro culture in tissue culture medium. The protein secreted by embryo were detected 48 hours after thawing for those frozen at morulla stage, and 24 hours and 72 hours after thawing for those frozen in the blastocyst stage. Results showed that preimplantation goat embryos secreted an average of 3,041 µg protein per ml medium for the embryo cultured in tissue culture medium (TCM) 199 + 0,1 % BSA and 1,992 µg protein per ml medium for embryo cultured in TCM without BSA. Non growing embryos secreted only a small amount protein, i.e. 0,434 µg protein per ml medium and 0,417 µg protein per ml medium respectively for embryo cultured TCM 199 with BSA and TCM without BSA. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed protein bands with many different molecular weight of 100, 95 , 55 , 43 , 28 and 18 KD. It appeared that the growing embryos secreted more proteins as compared to those non growing embryos.
Respon Tingkah Laku Birahi, Service Per Conception,Non Return Rate, Conception Rate pada Sapi Bali Dara dan Induk yang Disinkronisasi Birahi dengan Hormon Progesteron Mardiansyah, Mardiansyah; Yuliani, Enny; Prasetyo, Sugeng
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v2i1.24

Abstract

The aim of this study was to determine the differences ofestrous response, service per conception (S/C), nonreturn rate (NRR),and conceptionrate (CR) between Bali cattle heifersandcowssynchronized using intra-vaginalprogesterone hormone. Two groups of Bali cattle, 15 heifers and 15 cows, were used to examine the responsedifferences of estrous synchronization using progesterone hormone on reproductive performance, betweendifferent parity. Each group was given progesterone dose of 1 mg/kg body weight. The data observed wereestrousbehavior (estrousintensity, onset of estrous, estrous percentage), and value of S/C, NRR, and CR wereanalyzed by statistical analysis (t-test). The result shows the estrous intensity was significantly different (P<0.05)between the heifers and cows. The average estrousintensity score of the heifer was 2.87±0.35, while the cows was2.27±0.45. There was significantly different (P <0.05) in onset of estrous in the cows 41.5±5.18 hours, while inthe heifers was 50.67±3.5 hours. The estrouspercentage of the heifers and cows was the same, both of them was100%. There was no significant difference (P>0.05) in S/Cvalue showed by the heifers (1,31±0,45)and the cows(1,50±0,50). The average value of NRR, for heifers and cowswas 86.67% and 80.00%, respectively. The averagevalue of CRfor heifers and cowswas 86.67% and 80.00%, respectively.
Efektifitas Kinerja Pentoksifilin Terhadap Kualitas dan Integritas Membran Plasma Utuh pada Sperma Sapi Bali Hasil Pemisahan dengan Menggunakan Albumin Rodiah, Rodiah; Yuliani, Enny; Dradjat, Adji Santoso; Arman, Chairussyuhur
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 1 No 1 (2015): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v1i1.12

Abstract

The purpose of this research was  to determine the effect of Pentoxyfilline  in maintaining the quality (motility, viability, morphology and plasma membrane intact) of Bali bull spermatozoa separated with albumine.  A Completely Random Block Design was used in this study with two main factors (upper layer and bottom layer), diluents with four doses of pentoxyfilline (0, 15, 20  and 25 ?M) with six  replications.  Evaluation of spermatozoa was performed by characterizing motility, viability, morphology, plasma membrane intact. Result of the study showed that the effect of four doses  pentoxyfilline on the quality of Bali bull spermatozoa was non significant (p>0,05). However, the addition of  25?M pentoxyfilline was found to give the best effect compared to 15 ?M, 20 ?M and control. Result  of the study also indicated that spermatozoa separated in the upper layer was signifiqantly higher (p<0,01) than that of separated in the bottom layer with the average  motility, viability, morfology and  plasma membrane intact was 66,67,80,50, 6,71 and 65,61% respectively.
Identifikasi Komponen, Faktor dan Resiko Relatif yang Berpengaruh pada Hasil Inseminasi Buatan pada Sapi Bali Dradjat, Adji Santoso; Rodiah, Rodiah; Yuliani, Enny; Arman, Chairussyuhur
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v2i1.26

Abstract

The aims of the research were to identify components, factors and relative risk (Odd ratio) which may influencethe artificial insemination results. The study were performed in two villages those were Kuranji and Tanah Beak,by identification of components and factors which may contribute to successful artificial insemination results,then the flow chart of successful and obstacle were designed. Based on the flow chart survey was performed to thefarmers and inseminators. Parameters evaluated were factors may influence of the result of insemination such as;management, oestrus detection by farmers and insemination by inseminators. The data was tabulated and analisedusing epidemiology analysis of odd ratios (OR). The results showed that insemination results of Kuranji villagedo not have the risk to be not pregnant as the odd ratio was less than 1 (OR <1,0), with the range of 0,10 to 0,20.While artificial insemination in Tanah Beak village showed risk of cows to be infertile or non pregnant relativelyhigher with OR higher than 1 (OR> 1.0), with the range of 1,5 to 2,0. The results showed that the risk to beunsuccessful to produce pregnancy were caused by oestrus detection, feeding management, skill inseminator andexperience of farmers. Finally it can be concluded that oestrus detection by farmers, feeding management,insemination by inseminator and experience of farmers may contribut to the high risk fail to produce pregnancyusing artificial insemination in Tanah beak village.