Ervia Yudiati
Jurusan Kelautan Fakultas Perikanan dan Ilmu Kelautan UNDIP

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Profil Pigmen Polar dan Non Polar Mikroalga Laut Spirulina sp. dan Potensinya sebagai Pewarna Alami (Profile of Polar and Non-Polar Pigment from Marine Microalgae Spirulina sp. and Their Potential as Natural Coloring) Sedjati, Sri; Yudiati, Ervia; Suryono, Suryono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 3 (2012): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Penelitian pigmen Spirulina sp. dilakukan untuk menentukan kandungan pigmen polar dan non polar dari Spirulina sp. dengan metode Spektroskopi UV-Vis. Pigmen polar diekstraksi dengan menggunakan larutan buffer fosfat (NaOH-KH2PO4) pH 7, sedangkan pigmen non polar diekstraksi dengan menggunakan aseton murni. Hasil penelitian menunjukkan bahwa pigmen polar berwarna biru dan kadarnya adalah sebesar 42,272±0,05 mg/g (berat kering), pigmen ini terdiri dari fikosianin (29,159±0,29 mg/g), allofikosianin (9,363±0,20 mg/g), dan fikoeritrin (3,750±0,09 mg/g). Warna pigmen non polar adalah hijau terang dan kadarnya sebesar 4,498±0,06 mg/g (berat kering), pigmen ini terdiri dari klorofil a (3,349±0,03 mg/g) dan karotenoids (1,158±0,03 mg/g). Ekstrak pigmen polar Spirulina sp. kadarnya lebih tinggi dibanding pigmen non polarnya. Pigmen fikosianin berpotensi dikembangkan sebagai bahan aditif pewarna biru alami, karena: kadarnya paling tinggi, menghasilkan warna biru cerah dan cemerlang, bersifat nutrisi fungsional dan tidak beracun.Kata kunci: Spirulina sp., polar, non polar, pigmen, alamiResearch on pigments from Spirulina sp. powder has been done in order to determine polar and non polar pigments content by UV-Vis Spectroscopy method. Polar pigments were extracted using phosphate buffer (NaOH-KH2PO4) pH 7, while non polar pigments were extracted using pure aseton. The result showed that colour of polar pigment was dark blue and its content was 42,272±0,05 mg/g (dry weight), these pigments consist of phycocyanin (29,159±0,29 mg/g), allophycocyanin (9,363±0,20 mg/g), and phycoerythrin (3,750±0,09 mg/g). The colour of non polar pigment was light green and its content was 4,498±0,06 mg/g (dry weight), these pigments consist of chlorophyll a (3,349±0,03 mg/g) and carotenoids (1,158±0,03 mg/g). Due to the high content of pigments from Spirulina sp, showing a bright blue colour, having functional nutrition, and non toxic, the polar pigment phycocyanin have been found suitable for use as additif natural blue colorant.Key word: Spirulina sp., polar, non polar, pigment, natural
Dampak Pemaparan Logam Berat Kadmium pada Salinitas yang Berbeda terhadap Mortalitas dan Kerusakan Jaringan Insang Juvenile Udang Vaname (Litopeneus vannamei) Yudiati, Ervia; Sedjati, Sri; Enggar, Ipanna; Hasibuan, Irpan
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 14, No 4 (2009): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Uji toksisitas akut dilakukan pada juvenil udang vaname Litopenaeus vannamei dengan pemaparan jangka pendek (96 jam) berbagai konsentrasi logam kadmium. Uji statis digunakan sebagai teknik uji toksisitas. Tujuan dari studi ini adalah untuk menganalisis mortalitas dan kerusakan jaringan insang udang vaname yang didedah logam kadmium pada salinitas yang berbeda. Hasil peneltian menunjukkan toleransi udang vanamae terhadap logam kadmium menurun sejalan dengan penurunan tingkat salinitas. Tingkat toleransi yang rendah dan kerusakan jaringan terberat terjadi pada udang yang didedah pada salinitas 10 ppt (LC50 - 96 jam : 1,66 ppt Cd) diikuti berturut-turut salinitas 20 ppt (LC50 - 96 jam : 2,54 ppt Cd), 30 ppt (LC50 - 96 jam : 4,41 ppt Cd) dan  40 ppt (LC50 - 96 jam : 5,16 ppt Cd). Kata kunci : Kadmium, Mortalitas, Insang, Litopenaeus vannamei, salinitas Acute toxicity test was conducted on Litopenaeus vannamei on short term exposure (96 hours) to various concentration of cadmium at different salinity. A static test was applied as an acute toxicity test technique. The objective of this study was to assesst the mortality rate and the damage of gill tissue of L. vannamei exposed to cadmium at different salinity. Tolerance to these metal was decreased progressively according to the salinity level. The less tolerant salinity and the heaviest damage gill tissue was found at lowest salinity/10 ppt (96 h LC50 : 1.66 ppt Cd) and followed by 20 ppt (96 h LC50 : 2.54 ppt), 30ppt (96 h LC50 : 4.41 ppt), and 40 ppt (96 h LC50 : 5.16 ppt), respectively. Key  words : Cadmium, Mortality, Gill, Litopenaeus vannamei, salinity.
Pengaruh Aplikasi Probiotik Terhadap Laju Sintasan dan Pertumbuhan Tokolan Udang Vanamei (Litopeneus vannamei), Populasi Bakteri Vibrio, serta Kandungan Amoniak dan Bahan Organik Media Budidaya Yudiati, Ervia; Arifin, Zaenal; Riniatsih, Ita
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 15, No 3 (2010): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Peningkatan kadar amoniak dan bahan organik pada media budidaya udang vaname (Litopenaeus vannamei) merupakan salah satu faktor yang mempengaruhi keberhasilan budidaya. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian probiotik pengoksidasi amoniak dan bahan organik terhadap laju sintasan hidup dan pertumbuhan tokolan udang vaname, populasi bakteri vibrio serta kandungan amoniak dan bahan organic media budidaya. Penelitian dilakukan menggunakan metode eksperimental laboratories dengan empat perlakuan yaitu perlakuan pemberian 1 ppm  probiotik A (Nitrosomonas eutorpha MPN-8.2, dan Nitrobacter winogradskyi MPN-2), pemberian 0,1 ppm probiotik B (Paracoccus pantotrophus SB 3056 1,5x109cfu/g dan Bacillus megaterium SB 3112 2,5x109cfu/g), pemberian campuran probiotik A dan probiotik B dengan dosis masing masing 1 ppm  dan 0,1 ppm  serta control (tanpa probiotik). Tiap perlakuan diulang sebanyak tiga kali. Kandungan amoniak dan bahan organik dalam air diukur  pada hari ke 0, 15 dan 30. Hasil penelitian menunjukkan bahwa perlakuan pemberian probiotik A, B dan campuran probiotik A dan B berpengaruh : meningkatkan sintasan hidup tokolan udang vanamei yaitu berturut-turut (96%+3,05), (96,66+2,08)%, dan (97,33+1,25)% sedangkan kontrol (90,66 +3,30)%. (2) menurunkan populasi bakteri pathogen Vibrio, serta (3)menurunkan kadar amoniak dan bahan organik.Kata kunci: Litopenaeus vannamei, probiotik, amoniak, total bahan organic, sintasan The enhancement of ammonia and total organic matter level in water medium of vannamei’s (Litopenaeus vannamei) grow out  is one of the major problem.The aims of this research is to find out the effect of survival rate and growth in  vaneme’s juveniles by probiotic addition  This research was done by experimental laboratory with four treatments i.e.  A = juveniles + 1 ppm (Nitrosomonas eutorpha MPN-8.2, and  Nitrobacter  winogradskyi MPN-2 ), B = juveniles + 0,1 ppm (Paracoccus pantotrophus SB 3056 1.5x109cfu/g and Bacillus megaterium SB 3112 2.5x109cfu/g), C = juveniles + 1 ppm (Nitrosomonas eutorpha and Nitrobacter  winogradskyi) + 0,1 ppm (Paracoccus pantotrophus and Bacillus megaterium) and K= juveniles without probiotic addition, respectively. These treatments were replicated in three times. The measurements was regularly done by considering the culture condition. The result shows that those probiotic treatments give the better survival rate i.e. A(96%+3.05), B=(96,66+2.08)%,  C=(97,33+1.25)%  when  caompared  to  the  controle (90.66+3.30)%.  Furthermore,  the pathogenic Vibrio population of probiotic treatments were low when compared to the control. The level of total ammonia and organic matter in control was higher than the probiotic treatments. Key words: Litopenaeus vannamei, probiotics, ammonia, total organic matter
Pengaruh Pengurangan Konsentrasi Nutrien Fosfat dan Nitrat Terhadap Kandungan Lipid Total Nannochloropsis oculata Widianingsih, Widianingsih; Hartati, Retno; Endrawati, H.; Yudiati, Ervia; Iriani, Valentina R.
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 1 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Fosfat dan nitrat mempunyai peranan penting dalam pertumbuhan dan kandungan nutrisi Nannochloropsis oculata. Kandungan lipid total dalam Nannochloropsis oculata sangat dipengaruhi oleh nutrien yang terkandung dalam media kultur. Penelitian ini bertujuan untuk menganalisis pengaruh  perbedaan komposisi nutrien fosfat dan nitrat  terhadap kandungan lipid total  mikroalga Nannochloropsis oculata. Anova satu arah dengan 4 perlakuan dan 3 ulangan diterapkan pada penelitian ini.  Pelakuan perbedaan komposisi fosfat dan nitrat yaitu Kontrol (K, fosfat dalam NaH2PO4 20 g dan nitrat dalam NaNO3 100 g),  fosfat dan nitrat 75 % dari kontrol (A), fosfat dan nitrat 50 % dari kontrol (B), serta fosfat dan nitrat 25 % dari kontrol (C).  Pemanenan mikroalga untuk analisa  total  lipid  dilakukan  pada  fase  eksponensial  dan  stasioner.  Duaratus  limapuluh  mililiter  media bersalinitas 35 ‰ digunakan dalam penelitian ini dengan sistem aerasi dan pencahayaan 3000 lux yang kontinyu. Hasil penelitian menunjukkan bahwa perbedaan komposisi nutrien (fosfat dan nitrat) pada media pemeliharaan  berpengaruh  terhadap  kandungan  lipid  total  pada  fase  stasioner  namun tidak pada  fase eksponensial. Kandungan total lipid terbesar 67,7 % dw ditemukan pada N. oculata yang dikultur pada media dengan fosfat dan nitrat 25 % dari kontrol dan yang terkecil 39,3 %-dw pada N. oculata yang dikultur pada nutrien Kontrol.  Pembatasan nutrien pada media pemeliharaan dapat meningkatkan kandungan lipid total pada kultur  N. oculata Kata kunci: Nannochloropsis oculata, Total Lipid, fosfat, nitrat Phosphate and nitrate play  important role on growth and nutrition value of Nannochloropsis oculata.  Total lipid content of microalgae Nannochloropsis oculata is influenced by nutrient content in the culture medium. This research was aimed to examine the effect of different depletion phosphate and nitrate composition on the total lipid of  Nannochloropsis oculata.  Anova One Way with 4 treatments and 3 replicates has been applied in this research. The treatment of differences phosphate and Nitrate composition as follows Nutrient control (Conway medium having  Phospate in NaH2PO4 20 g and Nitrate in NaNO3 100 g), Nutrient A (phosphate and nitrate 75 % nutrient control); Nutrient B (phosphate and nitrate 50 % nutrient control), Nutrient C (phosphate and nitrate 25% nutrient control) The volume of culture medium was 250 mL with salinity 35‰, continuous aeration and illumination 3000 lux. The present work revealed that the nutrient composition on culture medium affected the total lipid content of N. oculata  at the stationary and but not in exponential phase. The highest total lipid content (67,7%-dw)  was found in N. oculata cultured in media with the lowest phosphate and nitrate concentration, in the contrary the lowest total lipid content (39,3%-dw) was happened in Control medium.  Nutrient limitation in medium culture was able to increase total lipid content in the culture of  N. oculata. Key words: Nannochloropsis oculata; Total  Lipid, phosphate, nitrate
Aktivitas Antioksidan dan Toksisitas Ekstrak Metanol dan Pigmen Kasar Spirulina sp. Yudiati, Ervia; Sejati, Sri; Sunarsih, Sunarsih; Agustian, Rani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 4 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Penelitian ini bertujuan untuk mengukur aktivitas antioksidan dan menguji toksisitas ketiga ekstrak. Penelitian dilakukan secara laboratoris. Ekstrak Kasar Spirulina sp didapatkan melalui proses maserasi dengan pelarut methanol. Ekstrak pigmen kasar diperoleh melalui partisi dengan pelarut methanol/aseton serta eter.  Aktivitas antioksidan dilakukan dengan mengukur IC50 dengan metode spektrofotometri dengan DPPH (1,1-diphenyl-2-picrylhydrazyl) sedangkan kadar toksisitas dilakukan dengan menghitung LC50 dengan uji BSLT (Brine Shrimp Lethality Test). Kromatografi Kolom Terbuka dilakukan untuk mengisolasi pigmen sedangkan identifikasi pigmen dilakukan dengan teknik Kromatografi Lapis Tipis dan metode spektroskopi. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa ekstrak kasar metanol, ekstrak pigmen kasar methanol/aseton dan eter mempunyai nilai IC50 323,7; 51 dan 34,85 ppm. Nilai LC50 dari ekstrak methanol r, ekstrak kasar pigmen methanol/aseton dan eter berturut-turut adalah 113,20; 65,22 ppm dan 34,11 ppm. Hasil isolasi pigmen dan identifikasi pigmen menunjukkan bahwa pigmen mengandung β-karoten dan klorofil α. Hasil uji fitokimia menunjukkan bahwa ketiga ekstrak positif mengandung senyawa golongan flavonoid dan sterol. Ekstrak pigmen kasar Spirulina sp. selain mempunyai aktivitas antioksidan yang tinggi juga mempunyai toksisitas yang tinggi terhadap nauplii Artemia sp. Kata kunci: Spirulina sp,ekstrak metanol, ekstrak kasar pigmen, IC50, LC50.  The aims of this research were to determine the antioxidant activities and level of toxicity of those three extracts. Spirulina sp crude extract has obtained by maseration technique with methanol solution while crude pigment extract has collected by partition technique with methanol/acetone and ether solution. The laboratory experiments of antioxidant activities (IC50) was determine by DPPH (1,1-diphenyl-2-picrylhydrazyl) and spectrophotometrical methods while the level of toxicity was done by BSLT (Brine Shrimp Lethality Test). The pigment was isolated using the Couloumb Chromatography Methods while pigment identification was done by Thin Layer Chromatography Methods and confirmed spectrophotometrically. The data was analized descriptively. The results showed that IC50 of methanol extract, crude pigment methanol/acetone and ether were 323.7; 51 and 34.85 ppm, respectively. Furthermore, the toxicity level LC50 of methanol extract, crude pigment methanol/acetone and ether were 113.20; 65.22 and 34.11 ppm respectively. β-carotene and chlorophyll α was identified and isolated. Those three extract contains flavonoid and sterol. It is concluded that crude pigment extract of Spirulina sp. has a high antioxidant activity as well as toxicity to the nauplii of Artemia sp. Key words: Spirulina sp.,methanol extract, crude pigment extract, IC50, LC50
Kandungan Klorofil-a pada Diatome Epipelik di Sedimen Ekosistem Mangrove Wibowo, Edi; Yudiati, Ervia; Suryono, Suryono; Retnowati, Tika
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 4 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Diatom Epipelik memiliki arti penting dalam sedimen mangrove karena merupakan komponen utama mikroalga yang mengandung klorofil, sehingga klorofil dalam sedimen akan mempengaruhi produktivitas primernya. Nilai kandungan klorofil -a dalam sedimen di kedalaman 2 cm berkisar antara 0,125 - 0,405 μg/cm3 menunjukkan bahwa pada kedalaman 2 cm mempunyai kandungan klorofil -a tertinggi bila dibandingkan pada kedalaman sampling 4 cm ataupun 6 cm. Untuk parameter fisika - kimia oseanografi, kondisi lingkungan memiliki kecenderungan yang normal.Kata kunci : klorofil -a, sedimen mangrove, produktivitas primer.The result showed that Epipelic Diatom have an important role in mangrove sediment, since they are known as major component of microalgae which containt chlorophy. These chlorophyll influence the level of primary productivity, within the sediment. The highest chloropyll a content within sediment was found in those taken from 2 cm deep range, compared with those taken from 4 cm and 6 cm deep.Key words : Chlorophyll -a, mangrove sediment, primary productivity.
Teknik Setting Spora Gracilaria gigas Sebagai Penyedia Benih Unggul dalam Budidaya Rumput Laut Yudiati, Ervia; Susilo, Endang Sri; Suryono, Chrisna A
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 1 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Rumput laut Gracilaria gigas dapat dikembangkan melalui cara generatif dengan cara menumbuhkan spora hingga menjadi thalus dengan teknik setting spora. Tujuan dari penelitian ini adalah mencari media yangtepat untuk tumbuhnya spora hingga menjadi thallus muda. Rancangan yang digunakan adalah rancangan acak lengkap dengan perlakuan media tempat melekatnya spora hingga menjadi thallus muda adalah talirafia, tali nilon, tali ijuk dan tali kapas. Hasil yang didapat menunjukan bahwa media yang paling banyak ditumbuhi oleh thalus muda adalah media dati tali rafia dengan kepadatan pertumbuhan 84 ind/cm2 sedangkan yang paling sedikit adalah media dari tali kapas dengan kepadatan pertumbuhan 24 ind/cm2.Kata kunci : Gracilaria gigas, setting spora, thallus, mediaSeaweed of Gracilaria gigas has developed by generative method with the concept to growing spores to be young thallus on the substrates. The aim of the research is to find the substrate which has comfortable sporesstick on to be young thallus. Randomized design was used in these experiment with four kind of rope (raffia, nylon, palm fiber and cotton) as a substrates. The highest number of young thallus was grew on raffia rope as substrate and the lowest was on cotton rope as a substrate.Key words : Gracilaria gigas, spora setting, thallus, media
Uji Toksisitas Ekstrak Kloroform Cangkang dan Duri Landak Laut (Diadema setosum) Terhadap Mortalitas Nauplius Artemia sp Aprilia, Hilda Ayu; Pringgenies, Delianis; Yudiati, Ervia
Journal of Marine Research Vol 1, No 1 (2012): Journal of Marine Research
Publisher : Journal of Marine Research

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Eggshells and spines of sea urchins (Diadema setosum) have toxins that can lead to certain biota surrounding death. Presumably the sea urchin has toxic compounds that can affect the surrounding environment. Because it needed a way to determine the toxic properties of sea urchin. Bioactive compounds contained in a previous study showing shells and spines of certain species of sea urchin pigment is coated by a black liquid that is stable. This fluid can be used as a mesh and skin coloring. It can also be used as fertilizer. This study aims to determine the lethal toxicity (LC50-24 h) of the chloroform extract of sea urchin of mortality Nauplius Artemia sp. The method used in this study is the experimental laboratory. Chloroform extract of the eggshells and sea urchin spines were tested using animal toxicity tests Nauplius Artemia sp.The results showed that the lethal toxicity (LC50-24 h) of the Nauplius Artemia sp in the chloroform extract of sea urchin eggshells at 133.58 ppm. As for the chloroform extract of sea urchin spines obtained results for 168.178 ppm. Chloroform extracts of both eggshells and sea urchin spines have potential activity as anti-microbial bio
KAJIAN HUBUNGAN FOSFAT AIR DAN FOSFAT SEDIMEN TERHADAP PERTUMBUHAN LAMUN Thalassia hemprichii DI PERAIRAN TELUK AWUR DAN PULAU PANJANG JEPARA Setiawan, Dedi; Riniatsih, Ita; Yudiati, Ervia
Journal of Marine Research Vol 2, No 2 (2013) : Journal of Marine Research
Publisher : Journal of Marine Research

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Seagrass growth is limited by the supply of nutrients such as nitrogen and phosphate particulates that serve as energy for photosynthesis. The magnitude of the role of phosphate in the metabolism and growth of seagrass Thalassia hemprichii important to serve as a study to determine the relationship of nutrients to the growing rate of seagrass in Teluk Awur and Pulau Panjang Jepara. The purpose of this study was to determine the concentration of phosphate in the water and sediment in the waters of the Teluk Awur and Pulau Panjang and its relation to seagrass leaf growth rate of T. hemprichii. The study was conducted in April - July 2012 includes literature studies, site surveys, field data collection and analysis of samples. Analysis of the relationship phosphate concentration on the rate of growth of seagrass T. hemprichii using bivariate correlation analysis. The results showed that the average growth rate - the average seagrass highest type T. hemprichii found in Teluk Awur on 8 week (0.81 cm/day). Meanwhile, the lowest growth rate found in Teluk Awur at week 2 (0.14 cm/day). The content of phosphate concentration of water in the Teluk Awur ranged from 0.009 to 0.028 mg/L and in Pulau Panjang ranged from 0.012 to 0.025 mg/L. The concentration of phosphate sediments in the Teluk Awur ranged from 20.870 to 23.250 mg/kg and Pulau Panjang 25.650 to 27.190 mg/kg. Relations water phosphate concentration and phosphate sediments to seagrass leaf growth in Teluk Awur are not considered closely related ,instead phosphate water to the growing rate of seagrass in Pulau Panjang is low and phosphate sediments to seagrass growth as very closely.
TRANSPLANTASI LAMUN Thalassia hemprichii DENGAN METODE JANGKAR DI PERAIRAN TELUK AWUR DAN BANDENGAN, JEPARA Wulandari, Dwi; Riniatsih, Ita; Yudiati, Ervia
Journal of Marine Research Vol 2, No 2 (2013) : Journal of Marine Research
Publisher : Journal of Marine Research

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The seagrass bed is a coastal ecosystem which have an important role for the coastal environment. The seagrass beds are vulnerable with changes of water environmental conditions. The decrease of seagrass beds area in the world are the result from environmental stresses both of natural and impact of human activities. Transplantation is one way of to rehabilitate the condition of seagrass beds were damaged. The purpose of this research was to determine the survival rate and the rate of growth of transplanted seagrass Thalassia hemprichii with anchor method at Teluk Awur and Bandengan Waters Jepara. The method used in this research was field experimental method. Determination of sites using purposive random sampling method. The research was conducted at two stations are Teluk Awur Water as Station I and Bandengan Water as Station II. Each station divided into 3 plots of observation. The environmental parameters were taken are: salinity, temperature, current velocity, depth, nitrate, phosphate, dissolved oxygen, organic matter, composition and grain size of the substrate. The results showed that the survival rate of seagrass transplants at Teluk Awur water was higher when compared to the survival rate of seagrass transplants at Bandengan water. The survival rate of seagrass transplants at Teluk Awur water have a range of of 38,89% to 41,67%, while the survival rate of seagrass transplants at Bandengan water have a range of 20,97% to 23,15%. The average range growth rate of seagrass transplants at Teluk Awur water was 0,13 cm/day to 0,16 cm/day, while the average range growth rate of seagrass transplants at Bandengan water was 0.16 cm/day up to 0.17 cm/day.