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Optimization of Production Xylanase from Marine Bacterium Bacillus safensis P20 on Sugarcane Baggase by Submerged Fermentation Rahmani, Nanik; Jabbar Robbani, Nadia Ulfa; Herawati Suparto, Irma; Yopi, Yopi
International Journal on Advanced Science, Engineering and Information Technology Vol 4, No 6 (2014)
Publisher : International Journal on Advanced Science, Engineering and Information Technology

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Abstract

Endo-1, 4-β-xylanase commonly called xylanase is an industrially important enzyme which degrades of lignocellulosic materials to sugar, alcohol and other useful product. The use of commercially xylan is too expensive for use at industrial scale production. For commercial applications, xylanases should ideally be produced from simple and inexpensive substrates. Indonesia has abundantly agro-residues such as sugar cane bagasse which is attractive to be used as carbon sources for the production of enzyme.  In this study, optimization of fermentation condition extracellular xylanasefrom marine bacterium, Bacillus safensisP20 has been conducted by using sugarcane bagasse as carbon source under sub merged fermentation (SMF). Maximum xylanase production was obtained at sugar cane bagasse concentration 1.5%, pH medium 7, and temperature fermentation 20oC, lactose as a carbone source and urea as a nitrogen source with activity 4.06 U/mL for 96 hours.
Bioethanol Production from Indica IR.64 Rice Straw Biomass by Direct Saccharification and Fermentation Betha Juanssilfero, Ario; Cameliawati Djohan, Apridah; Purnawan, Awan; Yopi, Yopi
International Journal on Advanced Science, Engineering and Information Technology Vol 5, No 1 (2015)
Publisher : International Journal on Advanced Science, Engineering and Information Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1068.124 KB) | DOI: 10.18517/ijaseit.5.1.467

Abstract

Lignocellulosic substances such as agricultural wastes are attractive feed stocks for bioethanol production. Indica IR.64 rice straw is one of abundant agricultural wastes in Indonesia and could be used to bioethanol production. It has several characteristics such as high content of cellulose and hemicelluloses that can be readily hydrolyzed into fermentable sugars. A simple process (the direct saccharification and fermentation process) to produce ethanol from rice straw was developed in order to establish an efficient bioethanol production. In this work, no harsh pre-treatment steps were applied and also use a simple one-vat reactor without the risk of losing liberated carbohydrate. The first step in using rice straw for bioethanol production is size reduction through milling and sieving process prior to enzymatic hydrolysis. Direct saccharification and fermentation (DSF) of Indica IR.64 rice straw was examined and compared with two type of control (systems devoid of yeast and enzyme). The experiment were carried out under anaerobic condition, where the cellulase crude enzyme and cellulosic substrates (rice straw) produced glucose from the cellulose and Saccharomyces cerevisiae directly assimilated the glucose to bioethanol. The faster rate of bioethanol production during DSF by Saccharomyces cerevisiae was obtained within the first 12h. The maximum ethanol concentration, ethanol yield, and theoretical ethanol yield of untreated rice straw were 0.25 g/L, 10 and 14.88%, respectively. Nevertheless, the direct saccharification and fermentation shows the potential for lower cost and higher efficiency for bioethanol production.
Substrates Preparation from Woody Tropical Waste Biomass for Biohydrogen Production Susilaningsih, Dwi; Harwati, Theresia; Anam, Khairul; Yopi, Yopi
Makara Journal of Technology Vol 12, No 1 (2008)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7454/mst.v12i1.140

Abstract

Addressing to the global warming problem, energy crisis and pollution, hydrogen production by micro-organisms using biotechnological approach should be considered, since it fulfils the recent society requirement to safely produce, renewable and environmental friendly energy. Hydrogen is one of the most promising green energy sources, because it is easily converted to electricity and cleanly combustible. There are three types of micro-organisms for hydrogen production, the first is cyanobacteria through the photosynthesis process, the second is anaerobic bacteria, which use organic substances as electron donor and energy and convert them to hydrogen, the third is photosynthetic bacteria, somewhat between photosynthetic and anaerobic bacteria, which are capable of converting the organic substances to hydrogen at a fairly high rate. We propose to use the abundant waste biomasses in Indonesia for hydrogen production by the microbial system. Our focus research is the production of hydrogen from waste biomasses by two-stage fermentation systems, which combine the conversion process of monomer biomasses to lactic acid by Lactobacillus sp. and the conversion process of lactic acid to hydrogen by photosynthetic bacteria. In this research, two kind substrates preparation were apply for woody waste biomass such as chemical hydrolysis and biological methods with several treatments. The results of the substrate preparation state showed that hydrolyses process of biomasses using strong acid are yielded total sugar about 70-90% of previous original content. Moreover, hydrolyses process using weak/diluted acid are yielded total sugar about 4-30% of original sugar. Furthermore, the biological treatments of degradation of woody waste biomasses are yielded total sugar about 0-10% (by single culture) and 10-50% (by consortium). Those hydrolysates substrates will use for fermentation two stages of lactate fermentation and conversion by photosynthetic bacteria in order to produce hydrogen gas.
SELEKSI DAN IDENTIFIKASI BAKTERI ENDOFIT POTENSIAL PENGHASIL ENZIM PROTEASE DARI TAMAN NASIONAL GUNUNG HALIMUN - (The Selection and Identification of Potential Endophyte Bacteria as Protease Enzyme Producer from Halimun Mount National Park) Melliawati, Ruth; Rohmattusolihat, Rohmattusolihat; Nuryati, Nuryati; Rahmani, Nanik; Yopi, Yopi
Biopropal Industri Vol 7, No 2 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Endophytic bacteria have an equal chance to bacteria that live outside the plant tissue as potential bacteria. The selection has done towards 326 bacterial endophyte isolates. This research aimed to find and identify proteolytic potential isolates. The proteolytic selection of endophytic bacteria had done using solid skim milk. The capability of endophytic bacteria to agglomerate milk was tested using liquid skim milk which incubated for 7 days at room temperature. Enzyme production of four selected isolates was made through fermentation in GYS medium. The results showed that 86 isolates have proteolytic potential. Isolate HL.29B.63 had highest protease enzymes activity (65.918 U/mL). Medium optimization was able to increase the enzyme activity into 89.94% (125.04 U/mL). The analysis used 16s rDNA showed that isolate HL.29B.63 was Bacillus amyloliquefacient subs. plantarum strain FZB42.Keywords: endophytic bacteria, fermentation, identification, protease, selection ABSTRAKBakteri endofit mempunyai peluang yang sama dengan bakteri yang hidup diluar jaringan tanaman sebagai bakteri potensial. Seleksi dilakukan terhadap 326 isolat bakteri endofit. Tujuan penelitian ini adalah mencari isolat yang berpotensi proteolitik dan mengidentifikasinya. Seleksi proteolitik terhadap bakteri endofitik menggunakan skim milk padat. Uji kemampuan bakteri endofitik dalam menggumpalkan susu menggunakan medium skim milk cair yang diinkubasi selama 7 hari pada suhu ruang. Produksi enzim terhadap empat isolat terseleksi dilakukan melalui fermentasi dalam medium GYS. Hasilnya menunjukkan bahwa 86 isolat mempunyai potensi proteolitik. Isolat HL.29B.63 mempunyai aktif enzim protease tertinggi (65,918 U/mL). Optimasi medium dapat meningkatkan aktivitas enzim sebesar 89,94% (125,04 U/mL). Analisis menggunakan 16s rDNA menunjukkan bahwa isolat HL.29B.63 adalah Bacillus amyloliquefaciens subs. plantarum strain FZB42.Kata kunci: bakteri endofit, fermentasi, identifikasi, protease, seleksi
Pertumbuhan Bakteri Laut Shewanella indica LBF-1-0076 dalam Naftalena dan Deteksi Gen Naftalena Dioksigenase - (The Growth of Marine Bacteria Shewanella indica LBF-1-0076 in Naphthalene and Naphthalene dioxygenase Gene Detection) Farini, Nuzul; Thontowi, Ahmad; Yetti, Elvi; Suryani, Suryani; Yopi, Yopi
Biopropal Industri Vol 8, No 1 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Crude oil exploitation which often occured offshore can cause water pollution in the sea since its contains naphthalene which is a hazardous compounds. This research used marine bacteria LBF-1-0076 that have ability in naphthalene degradation. This research aimed to study the parameter effect of naphthalene and cell concentration toward marine bacteria LBF-1-0076. This research also identified isolate LBF-1-0076 and detected the encode gene of naphthalene dioxygenase. Based on growth test result, the optimum naphthalene degradationby isolate LBF-1-0076 occured in 75 ppm naphthalene concentration with 15cell concentration. The result of 16S rDNA gene analysis showed that LBF-1-0076 was identified as Shewanella indica strain 0102 with identical value 99%. The result of naphthalene dioxygenase gene detection using Polymerase Chain Reaction (PCR) showed that the isolate contained naphthalene dioxygenase gene with size ±377 bp. Therefore, LBF-1-0076 potential as bioremediation agent to solve crude oil contamination in the sea.Keywords:   crude oil, marine bacteria, naphthalene, naphthalene dioxygenase, Shewanella indicaABSTRAKEksploitasi minyak bumi yang sering terjadi di laut mengakibatkan adanya pencemaran minyak di laut. Naftalena merupakan salah satu senyawa dominan berbahaya yang terkandung dalam minyak bumi dan dapat mengakibatkan pencemaran perairan. Penelitian ini menggunakan bakteri laut LBF-1-0076 yang memiliki kemampuan untuk mendegradasi naftalena. Tujuan dari penelitian ini adalah mempelajari pengaruh parameter konsentrasi naftalena dan konsentrasi sel terhadap bakteri laut pendegradasi naftalena LBF-1-0076. Penelitian ini juga bertujuan untuk mengidentifikasi isolat LBF-1-0076 dan mendeteksi gen pengkode naftalena dioksigenase. Berdasarkan hasil uji pertumbuhan, degradasi naftalena yang optimal oleh isolat LBF-1-0076 terjadi pada konsentrasi naftalena 75 ppm dengan konsentrasi sel 15. Hasil analisis gen 16S rDNA menunjukkan isolat LBF-1-0076 teridentifikasi sebagai Shewanella indica strain 0102 dengan nilai keidentikan 99%. Hasil deteksi gen naftalena dioksigenase dengan menggunakan Polymerase Chain Reaction (PCR) menunjukkan bahwa isolat tersebut mempunyai gen naftalena dioksigenase dengan ukuran ±377 bp. Oleh karena itu, isolat LBF-1-076 berpotensi sebagai agen bioremediasi untuk mengatasi masalah pencemaran minyak bumi di laut.Kata kunci: bakteri laut, minyak bumi, naftalena, naftalena dioksigenase, Shewanella indica
Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation Ratnakomala, Shanti; Yopi, Yopi; Suhartono, Maggy T; Meryandini, Anja; Prasetya, Bambang
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.031 KB) | DOI: 10.1234/96

Abstract

The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp.ID06-379. The study was focused on the influence of carbon, nitrogen,phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml),(NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28 C for 2 days incubation.
Pertumbuhan Optimal Bakteri Laut Pseudomonas aeruginosa LBF-1-0132 dalam Senyawa Piren Safitriani, Safitriani; Thontowi, Ahmad; Yetti, Elvi; Suryani, Suryani; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.628 KB) | DOI: 10.14203/jbi.v13i1.3100

Abstract

ABSTRACTPyrene is a high molecular weight chemical compound belongs to polycyclic aromatic hydrocarbon (PAHs) group that are difficult to degrade by environment. Biodegradation techniques using indigenous marine bacteria are used to be as an effort to reduce pollutants that are carsinogenic. The objectives of this research are to screen of 18 marine bacteria isolates qualitatively by sublimation method and quantitatively by growth test and to optimize degradation activity of marine bacteria isolates by pyrene concentration and cell concentration. Identification by 16S rDNA and phylogenetic tree analysis were conducted to determine the molecular basis of bacterial identity. The result of sublimation showed that 15 isolates were positive result for pyrene degradation and classified to 3 groups. The first group consisted of 5 isolates that can produce clear zone, while the second group are 5 isolates with isolate color changes. The third group have both of activities. Growth test showed that isolate LBF-1-0132 has high potency to degrade pyrene compound. Isolate LBF-1-0132 is capable of degrading pyrene compounds optimally at concentration of 600 ppm and optimum cell concentration of 20. Based on 16S rDNA gene analysis, isolate LBF-1-0132 is Pseudomonas aeruginosa with 98% identity.Keywords :pyrene, marine bacteria, optimization, 16S rDNA identification
Isolats Bakteri Indigenous Penghasil Milk-Clotting Protease untuk Fermentasi Keju Rahmani, Nanik; Sari, Yana Nurita; Palupi, Nurheni Sri; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.729 KB) | DOI: 10.14203/jbi.v9i2.166

Abstract

The aims of this research is to isolation of bacteria that potential to produce of milk clotting protease enzymes fromfermented food that will be used as a substitute for rennet in cheese making. There are five food fermentations suchas tauco, tempeh, red oncom, sticky tape, and pickled mustard greens that are used as a source for isolation of bacteriathat could produce milk clotting protease. The results obtained four isolates proteolytic bacteria from two fermentedfood samples, three isolates bacteria from tauco (TCN 1, TCN 2, TCN 3) and one isolate from pickledmustard greens (DSN 1). Based on 16S rDNA, these isolates were identified as Bacillus sp. Bacterial isolate TCN 1has a milk clotting activity of 29.17 U/mL, whereas bacteria isolates of TCN 2, TCN 3 and DSN 1 have activities of70 U/mL achieved at the 24 hours incubation, respectively. The proteolytic activities of bacteria isolates TCN 1,TCN 2, TCN 3 and DSN 1 at the 24 hours fermentation process were 0.0117 U/mL, 0.0021 U/mL, 0.0150 U/mL,and 0.200 U/mL, respectively. The ratio of milk clotting protease activity and the proteolytic activity for bacteriaisolates TCN 1, TCN 2, TCN 3 and DSN respectively were 5402, 175000, 7292, and 3333. This showed that theenzyme from bacterial isolates TCN 2 can be used as an alternative to rennin in cheese making.Keywords: milk clotting protease, cheese, calf rennet, fermentation food
Pemurnian Parsial dan Karakterisasi Amilase dari Bakteri Laut Arthrobacter arilaitensis LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1084.005 KB) | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it’s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
Karakterisasi Biodegradasi Senyawa Poliaromatik Dibenzothiophene Oleh Bakteri Laut Novosphingobium mathurense LBF-1-0061 Tanjung, Puspasari Noerwan; Yetti, Elvi; Thontowi, Ahmad; Suprihadi, Agung; Purwantisari, Susiana; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1056.931 KB) | DOI: 10.14203/jbi.v12i2.2894

Abstract

ABSTRACTDibenzothiophene is one of polycyclic aromatic hydrocarbon (PAH) compound containing sulfur element. This compound has toxicity, mutagenic and quiet persistent in environment. From sreening test, it was known that isolate LBF-1-0061 was potential to degrade dibenzothiophene. The objectives of this study are to study dibenzotiophene degrading capability by marine bacteria isolate LBF-1-0061 using screening test; analysis of dibenzothiophene residue by GC/MS and identifiy the isolate by molecular identification. The result of this research shown that LBF-1-0061 isolate could grow up to 100 ppm of dibenzotiophene. This isolate also presented degrading capability approximately 37.5% of dibenzotiophene in 14 days incubation. Based on partial 16S rRNA gene analysis, LBF-1-0061 was identified 99% as Novosphingobium mathurense strain SM117.Keywords: sea bacteria, biodegradation, dibenzotiofen, hydrocarbon aromatic polisiclic