Articles

Found 23 Documents
Search

Polyadenylphospate Ribose Polymerase (PARP) Activity Between Type 2 Diabetes Mellitus Patients Yerizel, Eti; Manaf, Asman; Yanwirasti, Yanwirasti; Oenzil, Fadil
Journal of the Indonesian Medical Association Vol. 62 No. 12 December 2012
Publisher : Journal of the Indonesian Medical Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Introduction: Type 2 diabetes mellitus is a degenerative disease which caused health problem inIndonesia and the world. In type 2 diabetes, it has been hypothesized that there its activation ofPolyadenylphosphate Ribose Polymerase (PARP) resulting in inhibition of Gliseraldehyde Phospate Dehydrogenase (GAPDH), endothelial dysfunction later it with cause endotheleal dysfunctions wich is the taste mechanisme of type 2 DM complications. This study aimed to assess difference in PARP activity mean between diabetes mellitus group and control group.Methods: This is an observational study using cross sectional method. Seventy subjects areenrolled according to inclusion and exclusion criteria, and divided into two groups using blocked randomization HbA1c level is tested by hemoglobin variant testing technique system. ELISA is used to examine the activity of PARP. Later, the data will be analyzed using t-test in SPSS.Results: The mean activity of PARP in the type 2 diabetes group was 457±81.34 units/mL, meanwhile in the non-DM group 214±75.54 units /mL, with p<0.05.Conclusions: There are significant mean differences of PARP activity between type 2 diabetesgroup and control group. J Indon Med Assoc. 2012;62:467-70.Keywords: type 2 diabetes mellitus, PARP activity, HbA1C
Kontribusi peroksidasi lipid terhadap kerusakan sel hati tikus putih akibat keracunan Aflatoksin B1 Yanwirasti, Yanwirasti
Jurnal Anatomi Indonesia Vol 1, No 2 (2006)
Publisher : Jurnal Anatomi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The experiment used 96 white rats (Rattus norvegicus) with age around eight weeks old and weight 180-200grams, divided into four groups of 24 rats each, based on the dosages ofAflatoxin B1 given. Each group wasdivided further into three subgroups of eight rats based on the length of exposure time ofAflatoxin1.Four dosages ofAflatoxin1 were administered orallyeveryday into different groups, consisted of 0 μg, 10 μg,15 μg, and 20 μg, dissolved in 0.2ml propylene glycol. Three subgroups received the dosage for 12 weeks, 16weeks, and 20 weeks.At the end of the experiment, the rats were sacrificed,malondialdehyde were analyzedusing Uchiyama and Mihara method. Liver cell damages were examined using histological slices stained byhaematoxilin eosin. Data was analyzed using analysis of variance, and P<0.05 was considered to be significantlydifferent.Data analysis shows that : 1) there were significant differences between the effects of 12 weeks and 20weeks exposure, and between dosage of 10 μg and 20 μg on increasingmalondialdehyde of liver tissue andliver cell damage. This also showed that increasing exposure time and dosages of Aflatoxin1 increasing inmalondialdehyde of liver tissue and liver cell damage. 2) There were no significant differences between dosages10 μg and 15 μg, between 15 μg and 20 μg, between 12 weeks and 16 weeks, and between 16 and 20weeks exposures, on increasingmalondialdehyde of the liver tissue. 3) themoreAflatoxin1 was given themoreliver cells damage. It is concluded that aflatoxin1may cause liver cell damage as the result of liver peroxidationand many other factors as well.
Hubungan Pengetahuan dan Dukungan Keluarga dengan Kepatuhan Minum Obat Anti Tuberkulosis di Puskesmas Andalas Kota Padang Siswanto, Ivan Putra; Yanwirasti, Yanwirasti; Usman, Elly
Jurnal Kesehatan Andalas Vol 4, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.155 KB)

Abstract

Abstrak Tuberkulosis merupakan masalah kesehatan dunia terutama di negara yang dikelompokkan dalam high burden countries termasuk Indonesia. Pada tahun 2012, Indonesia berada di posisi empat dengan jumlah penderita TB terbanyak di dunia. Berdasarkan laporan tahunan Dinas Kesehatan Kota Padang tahun 2010, kesembuhan di Puskesmas Andalas sebesar 75,9%. Angka ini masih dibawah target pencapaian nasional sebesar 85%. Pada tahun 2011, angka kesembuhan di Puskesmas Andalas naik cukup signifikan yaitu mencapai 88,24% yang melebihi target nasional. Tujuan penelitian ini adalah menentukan hubungan antara pengetahuan pasien TB paru dan dukungan keluarga terhadap kepatuhan minum obat  anti-tuberkulosis di puskesmas Andalas Kota Padang. Desain penelitian yang digunakan cross sectional study  dengan jumlah subjek sebanyak 26 orang penderita TB paru yang melakukanpengobatan di Puskesmas Andalas. Data dikumpulkan melalui wawancara kepada responden menggunakan kuisioner yang kemudian di analisis melalui uji chi-square dengan derajat kepercayaan 95%. Pada penelitian didapatkan hubungan antara pengetahuan pasien TB paru (p=0,000) dan dukungan keluarga (p=0,04)  dengan kepatuhan minum obat anti-tuberkulosis di Puskesmas Andalas Kota Padang.Kata kunci: tuberkulosis paru, kepatuhan, pengetahuan, dukungan keluarga Abstract Tuberculosis is a global health problem, especially in countries that are grouped in high-burden countries,including Indonesia. In 2012, Indonesia was in fourth position with the highest number of TB patients in the world.Based on the annual report of Padang City Health Department in 2010, succeed rate in Andalas Health Centre inPadang was 75.9% and this was below natonal target (85%). In 2011, succeed rate in Andalas Health Centre inPadang increased significantly, 88.24% which exceed national target. The objective of this study was to determine the relationship between knowledge of pulmonary TB patients and family support to adherence of anti-tuberculosis drugs at the health center patients Andalas Padang. The design of this study was cross sectional study. There were 26 subjects of pulmonary TB patient on treatment in Andalas Health Centre which were taken by total sampling. Data were collected through interview using questionnaire and analyzed by chi square test with 95% confidence interval.The results showed an association between patients knowledge (p-value: 0.000) and family support (p-value: 0.04) with medication adherence in Andalas Health Center, Padang City.Keywords: pulmonary tuberculosis, adherence, knowledge, family support
Gambaran Pengetahuan dan Sikap Mahasiswa Fakultas Kedokteran Universitas Andalas tentang Rokok Yosantaraputra, Yosantaraputra; Yanwirasti, Yanwirasti; Abdiana, Abdiana
Jurnal Kesehatan Andalas Vol 3, No 3 (2014)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.707 KB)

Abstract

AbstrakRokok merupakan penyebab kematian sepuluh besar di dunia dimana jumlah kematian mencapai 500 juta orang per tahun. Dalam setiap enam detik terdapat satu kematian akibat rokok.Rokok mengandung lebih dari 4000 senyawa kimia, dimana 60 diantaranya bersifat karsinogenik. Lebih dari 85% penderita kanker paru adalah perokok. Terdapat juga beberapa kanker lain yang ada hubungannya dengan rokok. Salah satu penyakit yang dapat timbul akibat asap rokok adalah leukemia. Disamping itu rokok dapat meningkatkan resiko penyakit kardiovaskuler.Tujuan penelitian ini adalah untuk mengetahui pengetahuan dan sikap mahasiswa Fakultas Kedokteran Universitas Andalas tentang Rokok. Penelitian ini dilakukan dengan metode penelitian deskriptif dan pengambilan sampel dengan menggunakan teknik Simple Random Sampling dengan jumlah sampel sebanyak 273 orang. Dari hasil penelitian, menunjukkan bahwa tingkat pengetahuan responden terhadap rokok berada dalam kategori baik sebesar 0,4%, kategori sedang sebesar 64,5%, dan kategori kurang sebesar 35,1%. Hasil uji sikap responden terhadap rokok berada dalam kategori baik sebesar 90,1%, kategori sedang sebesar 9,5% dan kategori kurang sebesar 0,4%. Dari hasil penelitian ini dapat disimpulkan bahwa pengetahuan mahasiswa Fakultas Kedokteran Universitas Andalas berada pada kategori sedang dan sikap mahasiswa Fakultas Kedokteran Universitas Andalas berada pada kategori baik. Disarankan kepada mahasiswa Fakultas Kedokteran Universitas Andalas agar mencari tahu tentang nikotin dan akibatnya secara mandiri sesuai dengan sistem pembelajaran PBL, serta segera diberlakukannya peraturan bebas asap rokok di lingkungan FK Unand.Kata kunci: pengetahuan, sikap, mahasiswa, rokok.AbstractCigarettes are the top ten causes of death in the world where each year the total of mortality rate reached 500 million people. In every six seconds there will be found a death caused by smoking. Cigarette contains more than 4000 chemical compounds, in which 60of the mare carcinogenic. More than 85% of lung cancer patients are smokers. There are also some other cancers that has relationships withcigarettes. One of the diseases that mayariseas a result of cigarette smokers leukemia. Besides that, smoking may increase the risk of cardiovascular disease. The purpose of this study was to determine the knowledge and attitude of students of the Faculty of Medicine, University of Andalas about Cigarettes.This is a descriptive research method and the sample with drawal is done by using Simple Random Sampling technique witha sample size of 273 people. The results of the study show that respondent’s knowledge towards Cigarette is the good category 0,4%, the average category 64,5%, and the less category 35,1%. The experiment’s result on respondent’s attitude to wards Cigarette is the good category 90,1%, the average category 9,5% and the less category 0,4%. From these resultsit can be concluded that the knowledge of students of the Faculty of Medicine, University of Andalas in average category and attitude of students of the Facultyof Medicine, University of Andalas are ingood category.It is recommended to students of the Faculty of Medicine, University of Andalas in order tofind out about nicotine and consequently independently in accordance with the PBL learning systems, as well as the enactment ofsmoke-fre elegislation in the Faculty of Medicine, University ofAndalas.Keywords: knowledge, attitude, students, cigarettes
Occult Infection pada Anak Setelah 10 Tahun Mendapat Imunisasi Hepatitis B Asrawati, Asrawati; Jurnalis, Yusri Dianne; Yanwirasti, Yanwirasti
Sari Pediatri Vol 17, No 5 (2016)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp17.5.2016.343-348

Abstract

Latar belakang. Occult hepatitis B infection (OBI) adalah suatu keadaan ditemukannya deoxyribose-nucleic acid (DNA) virus Hepatitis B (VHB) pada pemeriksaan HBsAg negatif. Mutasi pada preS2/S menyebabkan sekresi HBsAg menurun sehingga kadar HBsAg dalam serum terlalu rendah yang menyebabkan HBsAg tidak terdeteksi. Virus hepatitis B cenderung mengalami mutasi sebab tidak memiliki sistem koreksi selama replikasi karena tidak adanya 3’,5’exonuclease untuk mengkoreksi kesalahan insersi nukleotida saat transkripsi balik.Tujuan. Mengetahui insiden occult infection setelah 10 tahun mendapat imunisasi hepatitis B.Metode. Penelitian observasional dengan menggunakan desain cross sectional study pada anak sekolah dasar di Kota Padang dengan jumlah subjek penelitian 40 anak.Hasil. Jenis kelamin perempuan lebih banyak daripada laki-laki dan status gizi baik lebih banyak dari status gizi kurang. Insiden occult infection ditemukan 10% pada anak setelah 10 tahun mendapat imunisasi hepatitis B. Tidak terdapat hubungan antara antiHBs dengan occult infection.Kesimpulan. Occult infection pada anak setelah 10 tahun mendapat imunisasi hepatitis B adalah 10 %.
Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau Syamsurizal, Syamsurizal; Yanwirasti, Yanwirasti; Manaf, Asman; Jamsari, Jamsari; Parwanto, ML Edy; Sardi, Arif
Universa Medicina Vol 33, No 3 (2014)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.205-212

Abstract

BACKGROUNDThe prevalence of type-2 diabetes mellitus (T2DM) has been increasing rapidly in developed and developing countries. Many new loci associated with T2DM have been uncovered by genetic and genome-wide association studies, eg. the transcription factor 7-like 2 (TCF7L2) gene variant rs7895340 has been associated with type-2 diabetes mellitus. The aim of this research was to find an association between polymorphisms of TCF7L2 gene variant rs7895340 with T2DM in ethnic Minangkabau.METHODSThis was a case-control study using a consecutive sampling technique among ethnic Minangkabau patients who came for treatment to the Metabolic Endocrinology Polyclinic at Dr. M. Jamil Hospital. Analysis was performed on 132 subjects (66 T2DM patients and 66 controls). DNA was isolated from venous blood samples and amplified using the amplification refractory mutation system - polymerase chain reaction (ARMS-PCR) method to detect single nucleotide polymorphisms (SNPs) of TCF7L2 gene variant rs7895340. To determine the significance of association of SNPs of rs7895340 with T2DM in ethnic Minangkabau, data analysis was performed using chi-square test.RESULTSThe ARMS - PCR for TCF7L2 gene variant rs7895340 found polymorphisms in 28 (42.42%) subjects with type 2 diabetes mellitus and in 15 (22.72%) non-diabetic subjects.There was a significant association between rs7895340 SNPs with the probability of T2DM in ethnic Minangkabau (OR=2.505, p=0.026). CONCLUSIONSSingle nucleotide polymorphism rs7895340 of TCF7L2 gene can be used as genetic marker of type-2 diabetes mellitus in ethnic Minangkabau.
The effect of ethanol extract of myrmecodia pendens to TGF-β1 expression and osteoblast cells after tooth extraction (the experimental research on cavia cobaya) Ismardianita, Efa; Nasrul, Ellyza; Yanwirasti, Yanwirasti; Hemiawati, Mieke
Journal of Dentomaxillofacial Science Vol 2, No 3 (2017)
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v2i3.647

Abstract

Objective: Myrmecodia pendens is often used as a traditional medicine to treat various diseases. A previous study to ethanol extract of sarang semut plant (Hypnophytum furmicarum jack) 4.65 mg was proven effective in fastening establishment of socket granulation tissue after tooth extraction, the Myrmecodia pendens 3% also fasten the healing process of soft tissue wound after tooth extraction. The aim of this study is to determine the effect of ethanol extract of 10%  Myrmecodia pendens to the expression of TGF-β1, and the number of osteoblasts after tooth extraction.Material and Methods: this study used 24 male marmoots divided into 2 groups. The entire groups had been extracted in the 1st left incisor mandibula, into the socket control group applied 0,1 ml of 0.5% CMC. The treatment group was given 0,1 ml ethanol extract of Myrmecodia pendens 10% dissolved with CMC 0.5%. On day 3, 7, 14 and 21, marmoots were decapitated and histopathologic preparations then observed the expression of TGF-β1, and the number of osteoblast cell. Data was analyzed by Kruskall-wallis test with probability p<0.05.Results: Based on the Kruskal-wallis test, there was a significant difference of the TGF-β1 expression and the number of osteoblast cell which had giving ethanol extract of Myrmecodia pendens between the control group and group treatment (p<0.05).Conclusion: the ethanol extract of Myrmecodia pendans can help the wound healing process after tooth extraction by increasing the expression of  TGF-β1, and osteoblast cells number.
Pengaruh Pemberian Ekstrak Air Buah Pepaya Muda (Carica papaya.L) terhadap Gambaran Histologi Ovarium Tikus Betina (Rattus norvegicus) Satria, Okti; Yanwirasti, Yanwirasti; Amir, Anri
Jurnal Ilmiah Universitas Batanghari Jambi Vol 18, No 3 (2018): Oktober
Publisher : Universitas Batanghari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.587 KB)

Abstract

Papaya seed (Carica papaya L.) as antifertility has been known since 1970 can cause the quantity and quality of sperm decreased. This antifertility potency is reinforced by the existence of research showing that inside papaya seeds contain antifertility compound that is saponin. In young papaya fruit (Carica papaya L.) also contains saponins, alkaloids, minerals, vitamins, and enzymes. The latex sap from the young papaya fruit has the same influence as oxytocin on the uterus of both pregnant and non-pregnant rats (Gouado et al., 2007) this study was conducted to prove that in the water extract of young papaya fruit has antifertility effect. If this study is meaningful, it may be used as a reference for subsequent research on efforts to utilize bio-natural resources as a medicinal substance.The purpose of this study is to know the influence of young papaya fruit extract on overview of ovarium histology of female rat.This research is a quantitative research using post-test design only control group design. This research is experimental with design. The research has been conducted from November to December 2017. This research was conducted at the Regional Center of Veterinary Investigation and Testing (RSVIT) II of Bukittinggi. The population in this study was female rats with a total number of female rats during the study of 32 rats.The results showed that Fresh Papaya Fruit Extract significantly influenced the number of primary follicles and follicles of rat Atresia (Rattus novergicus) (p = 0,000).Young Pepaya Fruit Water Extract can be considered as an efficient natural contraceptive and a few side effects.
Karakteristik Pasien Penyakit Jantung Rematik yang Dirawat Inap di RSUP Dr. M. Djamil Padang Hasnul, Marhamah; Najirman, Najirman; Yanwirasti, Yanwirasti
Jurnal Kesehatan Andalas Vol 4, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.719 KB)

Abstract

Abstrak Penyakit jantung rematik (PJR) adalah penyakit jantung sebagai akibat adanya gejala sisa (sekuele) dari demam rematik (DR) yang ditandai dengan terjadinya cacat katup jantung. Tujuan penelitian ini adalah melihat karakteristik pasien penyakit jantung rematik.yang dirawat. Metode yang digunakan adalah deskriptif observasional dengan desain cross sectional study dan pendekatan retrospektif untuk menilai karakteristik pasien PJR di RSUP Dr. M. Djamil Padang dari Januari 2009 - Desember 2012. Populasi dan sampel sebanyak 54 sampel. Hasil penelitian menunjukkan distribusi pasien terbanyak pada kelompok umur 11-20 tahun (50%), perempuan (57,41%), tingkat pendidikan SD/sederajat dan SLTP/sederajat masing-masing 25,93%, pelajar/mahasiswa (53,70%), dan berasal dari daerah rural (70,37%). Sebagian besar pasien mengeluhkan keluhan utama sesak napas (62,96%). Hasil pemeriksaan elektrokardiografi memperlihatkan LVH sebesar 35,19% dan AF sebesar 27,78%. Berdasarkan pemeriksaan foto toraks umumnya pasien telah mengalami kardiomegali (92,59%). Berdasarkan pemeriksaan ekokardiografi yang paling banyak ditemukan adalah kelainan katup regurgitasi mitral (30,40%), derajat kerusakankatup berat (36,80%), dan fraksi ejeksi normal (72,97%). Pada penelitian ini dapat disimpulkan bahwa terdapat variasi karakteristik dari penderita PJR yang ada di RSUP Dr. M. Djamil Padang. Kata kunci: penyakit jantung rematik, karakteristikAbstract Rheumatic heart disease is a cardiovascular disease caused by delayed sequele of rheumatic fever characterized by heart valve damage. The objective of this study was to observe the characteristics of RHD patients.This study is a descriptive observasional with cross-sectional study design and the retrospective by observing the characteristics of RHD patients in RSUP Dr. M. Djamil Padang from Januari 2009 – December 2012. The population and sample of this study was 54 patients. The result of this study showed that majority of the patients at the age group 11-20 years (50%), female (57.41%), elementary education degree/equal and junior high/equal of each 25.93%, students/university students (53.70%), and come from rural areas (70.37%). Most patients complained experiencedbreathlessness (62.96%). The result of electrocardiography showed LVH is the the highest (35.19%) and AF 27.78%. Based on chest X-ray examination majority of the patients showed a cardiomegaly (92.59%). Based on echocardiography examination, the most heart valve damage is mitral regurgitation (30.40%), the severity is severe (36.80%), and the fraction ejection is in normal limit (72.97%). The conclusion of this research, there are variation in the characteristics of RHD patients in RSUP Dr. M. Djamil Padang.Keywords: rheumatic heart disease, characteristics
Faktor-faktor yang Memengaruhi Insiden Miopia Pada Siswa Sekolah Menengah Atas di Kota Padang Rahimi, Martga Bella; Yanwirasti, Yanwirasti; Sayuti, Kemala
Jurnal Kesehatan Andalas Vol 4, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.823 KB)

Abstract

Abstrak Miopia merupakan kelainan refraktif yang paling banyak ditemukan di dunia. Hampir 90% miopia terjadi di negara berkembang. Berbagai faktor diduga berhubungan dengan insiden miopia seperti jenis kelamin, indeks masa tubuh (IMT), aktivitas dekat (dengan atau tanpa layar), aktivitas jauh (dengan atau tanpa layar), sosioekonomi, orangtua menderita miopia, serta riwayat kelahiran prematur atau berat badan lahir rendah (BBLR). Tujuan penelitian ini adalah menentukan hubungan antara faktor-faktor tersebut dan insiden miopia pada siswa Sekolah Menengah Atas(SMA) di Kota Padang. Penelitian dilakukan pada siswa SMA di Kota Padang yang berusia 15-17 tahun, dengan desain case-control study, population based dengan jumlah sampel 140 orang, yang terdiri dari 70 orang untuk kelompok kontrol dan 70 orang untuk kelompok kasus. Dikelompokkan setelah menguji ketajaman visus. Kemudian dilanjutkan dengan pengukuran tinggi badan, berat badan, serta pengisian kuesioner. Selanjutnya, data diolah dengan uji statistik chi-square. Aktivitas dekat dengan layar, IMT dan aktivitas jauh dengan layar merupakan faktor dominan yang memengaruhi insiden miopia pada siswa SMA di Kota Padang. Dibuktikan juga bahwa jenis kelamin, sosioekonomi, orangtua menderita miopia, riwayat lahir prematur ataupun BBLR tidak  memengaruhi insiden miopia. Kesimpulan penelitian ini adalah ada hubungan positif antara faktor risiko dengan insiden miopia.Kata kunci: miopia, faktor risiko, pelajar SMAAbstract Myopia is the most common refractive error in the world. Almost 90% of myopia patients are found in the developing countries. There are several factors suspected to be related to the incidence of myopia, such as gender, body mass index, close-range activities (with or without screen), distance activities (with or without screen), socioeconomic state, history of myopia in parents, and history of prematurity or low birth weight. The objective of this study was to determine the relation of those factors and the incidence of myopia on senior high school students in Padang city.This study was conducted on senior high school students in Padang city aged 15-17 years old. This study used population based case control design with 140 samples, consisted of 70 students for the control group and 70students for the case group. The process of data collection was conducted by initially classifying the respondents into case or control group using visual acuity test. It was followed by measuring body height and weight and filling the questionnaire.  Finally, those data were analyzed by using the chi square statistic test.The result of this study proved that the close-range activities with screen, were the dominant variable influencing myopia in senior high school students in Padang city, followed by BMI and distance activities with screen. It was also proved that none of thegender, socioeconomic state, history of myopia in parents, and history of prematurity or low birth weight have any influence toward myopia incidence. Conclusion of this study is that a positive correlation between suspected factors with myopia incidence. Keywords: myopia, risk factors, high school students