Articles

Effect of Mamordica charantia L. Powder on Antioxidant Superoxide Dismutase in Liver and Kidney of Diabetic Rats WRESDIYATI, TUTIK; SINULINGGA, TEGUH SURANTA; ZULFANEDI, YOLI
HAYATI Journal of Biosciences Vol 17, No 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

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Abstract

 The status of antioxidant superoxide dismutase (SOD) was reported decreased in the liver tissues of diabetic experimental Macaca fascicularis. This study observed effect of Mamordica charantia on the status of SOD in the liver and kidney of diabetic experimental rats. The SOD was localized using immunohistochemical technique.  Male Wistar rats of negative control and diabetes mellitus (DM) group treated with 5 and 10% of M. charantia powder for 28 days. The DM condition was achieved by alloxan (110 mg/kg BW) induction. Charantia powder increased the status of antioxidant SOD in the liver and kidney of diabetic experimental rats. Aplication of M. charantia powder 10% gave better results than that of 5%. The results suggested that M. charantia powder can increase the status of antioxidant in the oxidative stress condition, such as diabetes mellitus.
The Utilization of Seaweed as a Source of Dietary Fiber to Decrease the Serum Cholesterol in Rats ASTAWAN, MADE; WRESDIYATI, TUTIK; HARTANTA, ANZS BUDY
HAYATI Journal of Biosciences Vol 12, No 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The cholesterol lowering effect of seaweed (Eucheuma cottonii) powder as a source of dietary fiber was evaluated in hypercholesterolemic rats. Four groups of five male Sprague Dawley hypercholesterolemic rats were fed a 0% cholesterol-0% seaweed powder (negative control); 1% cholesterol-5% seaweed powder; 1% cholesterol-10% seaweed powder; and 1% cholesterol-0% seaweed powder (positive control) for 35 days. Seaweed powder contained feed did not affect the growth of rats but significantly lowered the serum total cholesterol, LDL cholesterol, triglyceride, and atherogenic index. The lowest serum cholesterol was found in the hypercholesterolemic rats fed with 1% cholesterol-10% seaweed powder. The values of total-cholesterol, LDL-cholesterol, and triglyceride were 67.7, 33.0, and 47.3 mg/dl, respectively.
The Immunohistochemical Profile of Superoxide Dismutase (SOD) in the Liver Tissue of Hypercholesterolemic Rats WRESDIYATI, TUTIK; ASTAWAN, MADE; HASTANTI, LUSIA YUNI
HAYATI Journal of Biosciences Vol 13, No 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

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Abstract

This study was conducted to observe intracellular antioxidant cooper,zinc-superoxide dismutase (Cu,Zn-SOD) in liver tissue of rats under hypercholesterolemic condition by using immunohistochemical technique. A total of twenty male Wistar rats were used for this study. Those rats were divided into two groups; (i) control group and (ii) hypercholesterolemic group, which were fed died containing 1% cholesterol for eight weeks. Rat livers were taken at the end of treatment, and processed by using paraffin embedding standard method. The tissues were stained immunohistochemically to Cu,Zn-SOD. Observation of Cu,Zn-SOD content in the tissue was performed qualitatively in the cytoplasm and quantitatively in the nucleus of hepatocytes based on colour intensity of enzyme reaction product. The profile of antioxidant-Cu,Zn-SOD decreased (P < 0.05) in the hypercholesterolemic group compared to the control group. Key words: Superoxide dismutase (SOD), hypercholesterolemia, liver, rat
Antioxidant Activity Of Ginger (Zingiber Officinale) Oleoresin On The Profile Of Superoxide Dismutase (Sod) In The Kidney Of Rats Under Stress Condition Wresdiyati, Tutik; Astawan, Made; Muchtadi, Deddy; Nurdiana, Yana
Jurnal Teknologi Dan Industri Pangan Vol 18, No 2 (2007): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Stress condition has beeb reported to decrease intracellular antioxidant-superoxide dismutase(SOD) in the liver and kidneyof rats. This study was conducted to evaluate the antioxidant activies of ginger oleoresin on the profile of superoxide dismutase(SOD) in the kidney of rats under stress condition. The stress condition was achieved by five days of fasting together with swimming for 5 min/day. Ginger oleoresin was orally administrated in a dose of 60 mg/KgBW/day for seven days. Drinking water was provided ad libitum to all groups. The treatment of ginger oleoresin significantly decreased malondialdehyde (MDA) levels and increased SOD activity, as well as immunohistochemicall, increased the content of copper, zinc-SOD (Cu, Zn-SOD) in the kidney tissues compared to that of untreated group. The antioxidant content in ginger oleoresin such as shogaol, zingeron, and gingerol, etc. were shownto have activities in the kidney tissues of rats under stress condition that is increasunf the profile of SOD. Ginger oleoresin treatment in combination both before and after stress gave the best result. Key words : antioxidant, ginger oleoresin, kidney, stress, superoxide dismutase (SOD)
Antibacterial Activity of Water lily Seed Extract Toward Diarrhea-causing Pathogenic Bacteria Fitrial, Yuspihana; Astawan, Made; Soekarto, Soewarno S; Wiryawan, Komang G; Wresdiyati, Tutik; Khairina, Rita
Jurnal Teknologi Dan Industri Pangan Vol 19, No 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The objectives of this study was to observe antibacterial activities of water lily seed, against diarrhea-causing pathogenic bacteria as well as lactic acid bacteria; phytochemistry components in water lily seed and to evaluate each component’s activities against pathogen bacteria. Extraction of antibacterial components in the seed was done by fractional extraction methods using solvent based on its polar level, i.e. hexane, ethyl acetate and ethanol. The activities of each extract was tested by using diarrhea-causing bacteria, Enteropathogenic Escherichia coli K.1.1 (EPEC K1.1) and S. typhimurium with agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated with plate counting. Qualitative phytochemical tests were performed on all extracts. Fractionation was performed on extract with the largest antimicrobial activity by using thin-layer chromatography. The activities of each fraction were tested qualitatively by bio-autography method on thin layer chromatographic plates. The water lily seed had an antibacterial activity against EPEC K.1.1 and Salmonella typhimurium, especially in ethyl acetate extract. Ethanol extract had the same, yet lower activity. Ethyl acetate and ethanol extract of the seed did not show inhibition against the growth of lactic acid bacteria (Lactobacillus sp) and Bifidobacterium bifidum. The MIC and MBC values of the ethyl acetate extract on EPEC K1.1 were 0.89 (mg/mL) and 1.33 (mg/mL), respectively, while similar values of that on S. Typhimurium were 1.11 (mg/mL) and 1.33 (mg/mL¬), respectively. Phytochemistry components within ethyl acetate extract were alkaloids, flavonoids, tannins, glycosides, saponins, and triterpenoids. All fractions in the ethyl acetate extract had antimicrobial activities against EPEC K.1.1 and S. Typhimurium. These fractions were thought to inhibit the growth of the test-microbes by synergic action of each component.Key words: water lily seed, antibacterial, ethyl acetate extract
Anti Inflammation Activity of Ginger (Zingiber officinale) Oleoresin on Kidney of Rats Under Stress Condition Wresdiyati, Tutik; Astawan, Made; Adnyane, I Ketut Mudite
Jurnal Teknologi Dan Industri Pangan Vol 14, No 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The present study was conducted to observe the role of antioxidant content of ginger oleoresin (Zingiber officinale) on inflammation in the kidney of rats under stress condition, as an anti inflammation. A total of sixty male Wistar rats were used for this study. They were divided into twelve groups; (1) control group, without treatment of both stress and oleoresin, (2) stress group, was treated by stress only, without oleoresin treatment, while (3) and (4) are groups that were treated by stress and then fed by standard feed for three and seven days, without oleoresin. Group (5) to group (12) were treated by stress and then followed by treatment of oleoresin for three and seven days. The doses of oleoresin were 20, 40, 60, and 80 mg/kgBW/day. Stress condition was done by 5 days fasting and swimming for 5 minutes/day, while drinking water was provided ad libitum. The highest number of inflammatory cells in the kidney of rats was observed in the stress group. The treatment of oleoresin after fasting stress showed decreasing of the number of inflammatory cells in the tissues. The decreasing rate was higher in the higher dose of oleoresin. The treatment groups that showed the number of inflammatory cells not significantly different from that of control group are treated groups receiving oleoresin 60 mg/kgBW/day for seven days, and 80 mg/kgBW/day for three and seven days, respectively. These results showed that ginger oleoresin has anti inflammatory effect in the kidney of rats kept under stress condition. Key words : Oleoresin, ginger, inflammation, kidney, rat, stress.
KARAKTERISTIK KONSENTRAT PROTEIN TERIPANG PASIR (Holothuria scabra J.) DENGAN BAHAN PENGEKSTRAK ASETON Karnila, Rahman; Astawan, Made; Wresdiyati, Tutik
Jurnal Perikanan Dan kelautan Vol 16, No 02 (2011)
Publisher : Jurnal Perikanan Dan kelautan

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Abstract

Sea cucumber (Holothuria scabra J.) is a fishery that has a nutrientcontent and high economic value. This study aims to know: (1) nutrient content(proximate) sea cucumber fresh meat, (2) the yield of protein concentratemanufacture of sea cucumbers, (3) nutrient content of sea cucumber proteinconcentrate, and (4) total amino acid content of protein concentrates of seacucumber.Penelitian in five stages of the experiment: (1) preparation of meat freshsea cucumbers, (2) analysis of nutrient content (proximate) sea cucumber freshmeat, (3) the analysis of protein concentrate yield of seacucumbers, (4) analysis ofnutrient content (proximate) protein concentrate sea cucumbers, and (5) analysisof total amino acid content of sea cucumber protein concentrate. The resultsshowed nutrient content (proximate) sand sea cucumber fresh meat that is 87.03%water (bb): 76.64% protein (bk); 4.16% fat (bk), 14.34% ash (bk), and 4 , 93%carbohydrate (bk). The average yield produced in the manufacture of seacucumber protein concentrate was 9.87%. While the nutrient content (proximate)sea cucumber protein concentrate that is 8.97% water, 66.07% protein, 0.89% fat;11.53% ash, and 12.54% carbohydrate. The highest amino acid content in seacucumber protein concentrate is a proline (5.17%) and glutamic acid (3.23%).
Comparative Microanatomy of The Local Goat and Sheep Pancreas Islets With a Special Reference to The Distribution and Relative Frequency of Glucagon Producing Cells Adnyane, I Ketut Mudite; Novelina, Savitri; Sari, Dwi Kesuma; Wresdiyati, Tutik; Agungpriyono, Srihadi
Media Veteriner Vol 8, No 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.
Morphology and Histomorphometry of Testis And Epididymis of Kacang Goat (Capra sp.) and Local Sheep (Ovis sp.) Noviana, Citra; Boediono, Arief; Wresdiyati, Tutik
Media Veteriner Vol 7, No 2 (2000): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini bertujuan untuk mempelajari morfologi dan histomorfometri testis dan epididymis kambing kacang (Capra sp.) dan domba lokal (Ovis sp.) dengan umur 1-1,setahun. Secara makroskopik, keliling, berat dan volume testis kambing kacang lebih kecil (P
Disease Problems of Rhino Captivation Suzanna, Erna; Wresdiyati, Tutik
MEDIA KONSERVASI Vol 3, No 3 (1991): Media Konservasi
Publisher : MEDIA KONSERVASI

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Abstract

Perhaps, the Javan Rhino (Rhmocerossondaicus) is the most endangered species among large animals in the world. Only two population of ~t are known in the world: one in Indonesia and another in l&acute;ietnam. None exists in the zoos. The Javan Rh~no&acute;sc aptivityis an alternative conse~vatione ffort to ensure their existence In the world. But it is imperative to give a correct immobilization, stable preparation such as in the natural forest. and also disease monitoring. Medication and preventive medicine for the Javan Rhino are vely important. Some diseases which have been encountered in the zoos are a.0.: helminthiasis. endocarditls, coliform d~arrheah. epat~tsb iliari.abscesses and slun lacerations can result in septicaemia. The sources of diseases In captivity can be some kinds of foods and water, animal keepers, equipment, contaminated place, rats and mice, birds and insects.