Komang Gede Wiryawan
Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, IPB

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Isolasi dan Identifikasi Bakteri Asam Laktat dari Cairan Rumen Sapi Bali sebagai Kandidat Biopreservatif ISOLATION AND IDENTIFICATION OF ACID LACTIC BACTERIA FROM BALI CATTLE’S GASTRIC FLUID AS A POTENTIAL CANDIDATE OF BIOPRESERVATIVE Suardana, I Wayan; Suarsana, I Nyoman; Sujaya, I Nengah; Wiryawan, Komang Gede
Jurnal Veteriner Vol 8, No 4 (2007)
Publisher : Jurnal Veteriner

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Abstract

A study was conducted to isolate and identify of lactic acid bacteria originated from gastric fluid of bali cattle, and to determine their potential as the candidates of biopreservative. Lactic acid bacteria were isolated by culturing the gastric fluid of bali cattle in de Mann, Rogosa, Sharpe (MRS) medium; screening the bacteria, and identification of bacteria species by Analytical Profile Index (API) 50 CHL Kit. The results showed that, the new species of lactic acid bacteria were isolated and identified as Lactococcus lactis spp lactis 1 (SR21 isolate) and Lactobacillus brevis 1 (SR54 isolate) that have broad spectrum antimicrobial activities. It is clear from this study that a potential lactic acid bacteria producing antimicrobial agent can be isolated from the gastric fluid of bali cattle.
Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics Jannah, Siti Nur; Dinoto, Achmad; Wiryawan, Komang Gede; Rusmana, Iman
Media Peternakan - Journal of Animal Science and Technology Vol 37, No 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (771.819 KB) | DOI: 10.5398/medpet.v37i3.7969

Abstract

The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria  were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high  phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken. Key words: Cemani chicken, gastrointestinal tract, lactic acid bacteria, probiotic
Efek Ekstrak Daun Murbei Terfermentasi sebagai Komponen Pakan terhadap Performa Mencit Syahrir, Syahriani; Wiryawan, Komang Gede; Parakkasi, Aminuddin; Winugroho, Winugroho; Lini, Christina
Jurnal Sain Peternakan Indonesia Vol 5, No 2 (2010)
Publisher : Universitas Bengkulu

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ABSTRAK Kandungan nutien dan produksi daun murbei cukup tinggi sehingga berpotensi menjadi sumber pakan ternak. Namun, daun murbei mengandung senyawa aktif 1-deoxynojirimycin (DNJ), yang dapat menghambat hidrolisis disakarida menjadi monosakarida. Penghambatan tersebut dapat meningkatkan fermentabilitas pakan berserat dalam system rumen. Tujuan penelitian ini adalah untuk mengkaji dampak senyawa DNJ yang terkandung dalam ekstrak daun murbei (EDM) dalam sistem pasca rumen. Penelitian menggunakan mencit sebagai hewan model percernaan pasca rumen. Penelitian terdiri atas 6 perlakuan dan 4 ulangan. Perlakuan terdiri atas: P0 = Ransum semi purified (kontrol), P1 = P0 + padatan residu fermentasi in vitro, P2 = P1 + EDM dengan kadar DNJ ransum sebesar 0,03%, P3 = P1 + padatan residu fermentasi in vitro yang diberi substrat EDM dengan kadar DNJ ransum sebesar 0,03%, P4 = P1 + EDM dengan kadar DNJ ransum sebesar 0,06%, dan P5 = P1 + padatan residu fermentasi in vitro yang diberi substrat EDM dengan kadar DNJ ransum sebesar 0,06%. Hasil penelitian menginformasikan bahwa penambahan ekstrak daun murbei dalam pakan mengakibatkan penurunan bobot badan, konsumsi dan kecernaan pakan serta kadar glukosa darah mencit. EDM tidak terdegradsi sempurna dalam sistem rumen, sehingga masih berdampak negatif pada sistem pasca rumen. Namun, fermentasi EDM dapat mengurangi dampak negatif senyawa DNJ dalam sistem pasca rumen.Kata kunci: Ekstrak daun murbei, 1-deoxynojirimycin, residu fermentasi in vitro, mencit.
Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics Jannah, Siti Nur; Dinoto, Achmad; Wiryawan, Komang Gede; Rusmana, Iman
Media Peternakan - Journal of Animal Science and Technology Vol 37, No 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (771.819 KB) | DOI: 10.5398/medpet.2014.37.3.182

Abstract

The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria  were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high  phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken.Key words: Cemani chicken, gastrointestinal tract, lactic acid bacteria, probiotic
Detoksifikasi Mikotoksin Melalui Optimalisasi Fungsi Rumen dengan Pemberian Ragi (MYCOTOXIN DETOXIFICATION THROUGH OPTIMIZATION THE RUMEN FUNCTION BY YEAST) Pantaya, Dadik; Wiryawan, Komang Gede; Amirroenas, Dwierra Evvyernie; ., Suryahadi
Jurnal Veteriner Vol 17, No 1 (2016)
Publisher : Jurnal Veteriner

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Abstract

Mycotoxins are toxic metabolites produced by some fungal species commonly found in food and feed,particularly in cereals. In intensive production systems, dairy cattle are commonly fed with cereal-richdiets and, consequently, are more exposed to micotoxins. Besides, such diet is often associated with ahigher risk of rumen acidosis which can also affect the performance and the helath of animal. In addition,the efficacy of microbial detoxification can be reduced during acidosis. For instance, some authors observeda decrease in the number of protozoa that are responsible for the degradation of some mycotoxins. Anotherconsequence of acidosis is the potential modification of ruminal absorption of mycotoxins, which until nowhas received scarce attention. Yeast Saccharomyces cerevisiae, probiotic additives have been shown toreduce the post-feeding drop in rumen pH and to increase the number of ruminal protozoa. This effect canbe positive in reducing the absorption and toxicity of mycotoxins in ruminantia.
Efektivitas Minyak Ikan Lemuru Terproteksi Terhadap Populasi Mikrob Rumen dan Fermentasinya pada Kerbau dan Sapi (THE EFFECT OF PROTECTED LEMURU FISH OIL ON RUMEN MICROBES AND ITS FERMENTATION IN BUFFALOES AND CATTLE) ., Yurleni; Priyanto, Rudy; Gurnadi, Eddy; Wiryawan, Komang Gede
Jurnal Veteriner Vol 14, No 3 (2013)
Publisher : Jurnal Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The objective of this experiment was to examine the effect of protected lemuru fish oil on rumenmicrobes and its fermentation  in buffalo and cattle fattened in feedlot.  Six  male buffaloes and eight malecattle aged between 1,5-2 years old with initial live weight of 218,66±16,28 kg and 217,37±15,44 kg,respectively, were used in this study. They were fattened in feedlot using 35% forage and 65% concentratediet for 2,5 months. The protected lemuru fish oil was in the form of dried carboxylate salt mixture (DCM)and given in the form of concentrate mixture,  45 g DCM/kg concentrate. The feeding treatments includedPO (forage+concentrate) dan P1 (forage+concentrate+DCM). At the end of feeding trial, the animals wereslaughtered and the rumen liquor were collected to analyze rumen microbes and its fermentation.  Thedata were  analyzed using a completely randomized design with 2x2 factorial models, feeding trial (PO and P1) as the first factor  and  animals as second factor (buffaloes and cattle). The results showed that nointeraction was found between the two factors on  rumen pH, NH3 concentration, partial VFA, and rumenmicrobes. There were interaction effect of  diets treatment and animal species on total VFA. Total VFAconcentration in buffaloes  with DCM supplementation was significantly higher (P<0,05) than cattle  withor without DCM supplementation. DCM supplementation decreased rumen pH and increased total VFA.The buffaloes had significantly higher total VFA, proteolitic bacteria, and butiric acid,  but  had lowerrumen pH and protozoa population compared to those of cattle. It is concluded that DCM supplementationcan increase the effectivity of   protected fish oil concentration and proportion of VFA as well as rumenmicrobial population.
The Effect of Different Energy Level on The Ration to The Mating Acceleration of Local Sheep Khotijah, Lilis; Maulidina, A..; Satoto, Kukuh Budi; Wiryawan, Komang Gede
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol 16, No 3 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (954.17 KB) | DOI: 10.29244/jintp.16.3.52-55

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The aim of this research was to find the appropriate ration energy levels to determine the puberty time of ewes.  The study used 12 offspring female sheep obtained from crossing Jonggol ewes of Animal Science Teaching and Research Unit (JASTRU) Faculty of Animal Science IPB with Garut male sheep.   The animal age was approximately 2-3 months with the average body weight 9.79 ± 1.97 kg.   The treatments were consisted of 3 rations which have different TDN level, namely: T1= 65% TDN, T2=70% TDN and T3= 75% TDN.  The experimental design used was a completely randomized design with three treatments and four replications.   The measured parameters were feed intake, average daily gain, and the first matting age and body weight.   The data obtained were analyzed using Analysis of Variance.   The results indicated that the ration energy levels did not significantly affected on the feed intake, average daily gain, as well as the first mating age and body weight.  The age of first mating was 180-203 days with body weight at 18-22 kg. It is concluded that the TDN level between 65% -75% has similar response to accelerate the mating of local sheep.
THE ADDITION MANGOSTEEN PERICARP MEAL AND VITAMIN E IN THE DIET ON DIGESTIVE ORGANS, ACCESSORY ORGANS, REPRODUCTIVE ORGANS, AND CARCASS OF LAYING HENS Mutia, Rita; Rusli, Ridho Kurniawan; Wiryawan, Komang Gede; Toharmat, Toto; Jakaria, Jakaria
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.21059/buletinpeternak.v41i3.17311

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This research aimed to study long-term addition natural herbal: mangosteen pericarp meal and non-herbal: vitamin E in the diet on digestive organs, accessory organs, reproductive organs, and carcass of laying hens. A total of 160 laying hens of Lohman strains (24-wk-old) the observation was conducted for 11 weeks. Designs used are a completely randomized design with four treatments and four replications (10 birds each). The treatments consisted of R0 (control diet), R1 (R0 + 1 g MPM/kg ration), R2 (R0 + 2 g MPM/kg ration) and R3 (R0 + 200 mg VE/kg ration). The variabel observation were digestive organs (percentage of crop, heart, gizzard, spleen, kidney, small intestine), accesory organs (percentage of liver and bile), reproductive organs, body weight and carcass of laying hens. The results showed that all of treatments (RO, R1, R2, and R3)  did not affect (P>0.05) internal organs (percentage of crop, heart, gizzard, spleen, kidney, small intestine), accesory organs (percentage of liver and bile), reproductive organs, body weight and carcass of laying hens. In conclusion, long-term addition of mangosteen pericarp meal 1-2 g/kg ration and vitamin E 200 mg/kg in the diet no adversed effect on digestive organs, accesory organs, reproductive organs, body weight and carcass of laying hens.
PENGARUH SUPLEMENTASI TEPUNG KULIT MANGGIS DAN VITAMIN E DI DALAM RANSUM AYAM RAS PETELUR STRAIN LOHMANN TERHADAP KUALITAS FISIK TELUR YANG DISIMPAN PADA WAKTU DAN SUHU YANG BERBEDA Mutia, Rita; Rusli, Ridho Kurniawan; Wiryawan, Komang Gede; Toharmat, Toto; Jakaria, Jakaria
Buletin Peternakan Vol 41, No 1 (2017): BULETIN PETERNAKAN VOL. 41 (1) FEBRUARI 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.21059/buletinpeternak.v41i1.22453

Abstract

Penelitian ini bertujuan untuk mengkaji pengaruh suplementasi tepung kulit manggis (TKM) dan Vitamin E (VE) di dalam ransum ayam ras petelur terhadap kualitas fisik telur selama penyimpanan pada waktu dan suhu yang berbeda. Ayam petelur diberi perlakuan pakan yaitu : R0 (ransum kontrol), R1 (R0 +1 g TKM/Kg ransum), R2 (R0 + 2 g TKM/Kg ransum) dan R3 (R0 + 200 mg VE/Kg ransum). Rancangan yang digunakan adalah rancangan acak lengkap pola faktorial 4 x 3 x 2 dengan 4 ulangan. Faktor pertama yaitu ransum, faktor kedua yaitu: waktu penyimpanan (1, 2 dan 3 minggu) dan faktor ketiga yaitu suhu penyimpanan pada refrigerator (4,13oC dan 22,50%) dan suhu ruang (27, 47oC dan 76,17%). Variabel yang diamati adalah: berat telur, persentase kerabang telur, persentase kuning telur, persentase putih telur, tebal kerabang, warna kuning telur dan Haught unit (HU). Hasil penelitian menunjukkan bahwa interaksi antara ransum, waktu dan suhu selama penyimpanan sangat nyata (P<0,01) menurunkan warna kuning telur. Waktu dan suhu penyimpanan sangat nyata (P<0,01) menurunkan HU dan meningkatkan persentase kuning telur. Interaksi waktu dan suhu penyimpanan nyata (P<0,05) menurunkan persentase putih telur. Tebal kerabang nyata (P<0,05) meningkat dipengaruhi oleh ransum dan sangat nyata (P<0,01) meningkat dipengaruhi oleh waktu penyimpanan. Berat telur dan persentase kerabang telur tidak dipengaruhi oleh ransum, waktu dan suhu penyimpanan. Kesimpulan penelitian adalah suplementasi tepung kulit manggis dan Vitamin E di dalam ransum ayam ras petelur strain Lohmann secara umum tidak mempengaruhi kualitas fisik telur (kecuali warna kuning telur dan tebal kerabang) yang disimpan pada waktu dan suhu yang berbeda. Kualitas fisik telur lebih utama dipengaruhi oleh waktu dan suhu penyimpanan yang berbeda. Suhu dan kelembaban terbaik untuk penyimpanan telur adalah 4,13oC dan 22,50% pada refrigerator, pada kondisi ini telur dapat disimpan selama 21 hari.