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Pengaruh Konsentrasi Besi dalam Larutan Hara terhadap Gejala Keracunan Besi dan Pertumbuhan Tanaman Padi

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

Iron (Fe) toxicity is a major constraint in rice production that decreases yield due to high level of soluble Fe. The aims of this experiement were to study the effect of particular Fe concentrations in nutrient solution on rice growth, and to determine Fe concentration in nutrient solution that caused light, moderate, and severe Fe toxiciy symptom. The experiment was conducted in a greenhouse, Bogor Agricultural University from May to July 2010. A randomized block design with two factors and three replications were used in this study. The first factor was Fe concentration in the medium solution (2, 50, 100, 200, 400, 600 ppm Fe), and second factor was rice genotypes (IR64, Margasari). The results showed the higher Fe concentration in the solution resulted in higher scores of iron toxicity symptoms, higher Fe levels in the plant, and caused stunted growth of rice plants. Levels of Fe in a solution of ≥ 200 ppm Fe inhibited plant growth. Iron toxicity symptom in Margasari was lower than the IR64 varieties, especially at concentrations of 200 and 400 ppm. According to the regression equation Y = 0.022X+ 1849, Fe concentration in the solution which caused light Fe toxicity symptom (score ≤ 3) was ≤ 52 ppm Fe, moderate (score = 5) was 143 ppm Fe, heavy (score = 7) was 234 ppm Fe, and severe (score ≥ 9) was ≥ 325 ppm Fe. Keywords: iron toxicity symptoms, iron concentration, rice

Analisis Marka RAPD yang Te rpaut dengan To leransi terhadap Naungan pada Kedelai

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of the research was to identify RAPD markers linked to QTL related to agronomic traits of soybean under low-light intensity condition.  The genetic material used in the QTL analysis based on RAPD Markers were  Ceneng, Godek, and  F6 RILs derived from hybridization between  Ceneng (tolerant parent) and Godek (sensitve parent). The results of molecular analysis showed that 9 primers were polymorphic and linked to the tolerant parent.  Primers produce 14 RAPD markers which were polymorphic and linked to the tolerant parent. The markers were distributed into a linkage group that containing seven markers. RAPD Markers (OPE15-800, OPM20-800) were linked to two QTL controlling number of productive node and seed weight, respectively.  The marker linked to the tolerant parent could be used as a marker assisted selection for high-yielding soybean lines under low-light intensity.    

Keragaman Karakter Komponen Hasil dan Hasil pada Genotipe Kedelai Hitam

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 3 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

The objective of this research was to determine the trait variance in black soybean lines in terms of yield and yield components. The materials used were F8 lines generated from hybridization of two local varieties, i.e. black seeded Ceneng and greenish yellow seeded Godek, and then selected under single seed descent method, except SC-39-1, SC-62-2 and GC-74-7 that were generated through bulk selection method. The results showed that the traits between plant genotypes were significantly different, except number of productive node and number of unfilled pod. Total variance was contributed more by number of filled pod, seed weight per plant and 100 seed weight. Seed weight per plant and 100 seed weight had high heritability with genetic variance and broad-sense heritability which were significantly different from zero. These characters are important for further selection to improve yields of black soybean lines.Keywords: black soybean lines, broad sense heritability, total variance

Teknik perbanyakan cepat sumberdaya genetik iles-iles untuk mendukung percepatan komersialisasi secara berkelanjutan

Jurnal Ilmu Pertanian Indonesia Vol 14, No 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Increasing demand on glucomannan as healthy diet in develop countries, promotes higher planting area of Amorphophallus. Iles-iles corm (Amorphophallus muellen) contains high percentage of glucomannan. In order to meet with increasing demand, providing mass planting materials is a great concern in Indonesia. Therefore, the study on skin corm and bulbil as propagation materials was conducted. In the first experiment, bulbil with different size and section, i.e., very small, small, medium and large, and half section and 1f4 section were used. In the second experiment, we evaluated the prospect of skin corm as propagul where different size of skin corm was used, i.e., 1 em x 1 em, 2 em x 2 em, and 4 em x 4 em. Results showed that both bulbil and skin corm could be utilized as prospectivepropagules of iles-iles. Propagation using bulbil was more superior as compared to skin corm. Bulbil of both whole and sectioned had high emergence of bud (> 90%), except very small sized ones. Very small bulbil (diameter less than 1 em) resulted in ca 605 of emergence. Skin corm sized 4 em x 4 em produced equal emergence to medium bulbil. In general, increasing size of skin corm increased success of emergent bud. Delaying emergence on small sized skin corm could be assessed by smaller nutrient reserved. Most unseccessemergence of small sized skin and very small bulbil was due to decay of the propagules. These experiments implied that both bulbils and skin corm could be used as mass propagules for iles-iles production. Keyword : Bulbi I, Amorphophallus muelleri, iles - iles, mass propagation, skin of corm.

Characterization of cDNA for PMT: a Partial Nicotine Biosynthesis-Related Gene Isolated from Indonesian Local Tobacco (Nicotiana tabacum cv. Sindoro1)

HAYATI Journal of Biosciences Vol 20, No 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Nicotine is the major alkaloid compound in cultivated tobacco (Nicotiana tabacum) that could potentially be converted into carcinogenic compound (nor-nicotine). The PMT gene encoding putrescine N-methyltransferase (PMT) is one of the two key genes that play a prominent role in nicotine biosynthesis. The aimed of this study was to isolate and characterize the cDNA sequence originated from Indonesian local tobacco cv. Sindoro1 (Ntpmt_Sindoro1). The results showed that the Ntpmt_Sindoro1 was 1124 bp in length. This cDNA fragment encodes for 374 amino acid residues. The predicted polypeptide from the cDNA is a hidrophilic protein, and has a predicted molecular weight of 40.95 kDa. The predicted amino acids sequence also showed high similarity to the PMT gene product Nicotiana sp. available in the GenBank data base. The amino acid sequences also exert conserved residues specifically exhibited only by PMT gene originated from N. tabacum. Clustering analysis revealed that Ntpmt_Sindoro1 belongs to the same clade as the PMT3 gene, a member of the N. tabacum PMT gene family. The Ntpmt_Sindoro1 cDNA sequence covering exon1-exon8 of the PMT gene fragment has been registered in the GenBank data base, under the accession number JX978277.

Evaluasi Ketenggangan Galur Padi Gogo terhadap Cekaman Aluminium dan Efisiensi Penggunaan Hara Kalium

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 30, No 2 (2002): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

 A study was conducted to reevaluate 15 upland lines previously selected in a field experiment and nutrient culture experiment or tolerance to aluminum toxicity and potassium efficiency in the plastic house of Center for Crop Improvement Studies, Tajur, Bogor. The experiment was conducted in pot culture using Red Yellow Podzolic soil from Jasinga, Bogor. Two level of AI stress (no AI stress and AI stress with pH 4.8, Al saturation 80%, and exchangeable Al 16.6 mei/IOOg) and two levels of potassium fertilizer (6 kg K2Olha and 60 K2Olha) were applied. The study showed that the upland rice lines performed poorer under AI-tress condition compared to under non stress condition in plant heigl at harvest, total number of  tiller, number of productive tiller, length of spikelet and grain weight/pot. The performance of the upland rice lines was also significantly different under different level of potassium fertilizer in total number off tiller and nun1ber of productive tiller. Lines that showed significant difference in its performance under AI-stress and non stress condition were consiidered as tolerant lines. The result showed that Lalantik Bamban, Sigundil,CT65 I 0-24-3- I, Hawara Bunar, Ketombol, and Grogol were classified as AI-tolerant and K-efficient lines. Key word: Upland rice, Aluminium, Potasium

Pemilihan Karakter Agronomi untuk Menyusun Indeks Seleksi pada 11 Populasi Kedelai Generasi F6

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 34, No 1 (2006): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

High-yielding variety is one of the targets in soybean breeding program. High-yielding variety could be developed through simultaneous selection. Simultaneous selection  using selection index was more efficient  than selection based on an individual trait only.  Objective of  the research was to select agronomic characters for construction selection index  on 11 soybean populations F6 generation based on correlation analysis, path analysis, and heritability.  The correlation analysis  showed that  number of branch,  number of total nod, number of filled pod, number of unfilled pod, number of total pod, and percentage of filled pod have positively and significantly correlated with  yield per plant.  Based on  path analysis, characters number of branch, number of unfilled pod, and number of filled pod have positive direct on yield per plant.   The result also showed that  number  of filled pod has maximum positive direct effect on yield per plant.  Generally, number of total nod, number of filled pod, number of total pod, and percentage of filled pod have higher heritability  than other characters.   Key words: Soybean, correlation, path analysis, heritability, selection index

Anther Culture Ability from Crossess Between Upland and New Plant Types of Rice

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 2 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Anther culture provides rapid route in obtaining pure lines in a single generation through producing green haploid plants that may be spontaneously doubled. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of eight F1s derived from crossess between upland and new plant types of rice and from their four parents through anther culture. Completely randomized design with 25 replications was used in this research. Treatments consisted of four parent lines/varieties i.e. P1 (Fatmawati and BP360E-MR-79-2), P2 (Fulan Telo Gawa and Fulan Telo Mihat) and eight F1s obtained from reciprocal crosses of P1 and P2. Callus induction medium was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1  kinetin + 10-3  M Putrescine, while regeneration medium was based on MS + 0.5 mg L-1  NAA + 2.0 mg L-1  kinetin + 10-3  M Putrescine. The result indicated that F1 derived from Fatmawati x Fulan Telo Gawa (5.00% green plants per total anther) and their reciprocal (3.80% green plants per total anther) crosses were the most responsive genotypes in rice anther culture (had high anther culture ability). The F1 genotypes were more effective to produce green and doubled haploid plants in rice anther culture than their parents. From this research, 161 double haploid plants (29.81%) from total acclimated green plantlets were obtained.  Keywords: anther culture, upland rice, new plant type of rice

Pendugaan Parameter Genetik dan Seleksi Galur Mutan Sorgum (Sorghum bicolor (L.) Moench) di Tanah Masam (Estimation of Genetic Parameters and Selection of Sorghum Mutant Lines under Acid Soil Stress Conditions)

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 3 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

  Sorghum is one of high value commodities for food and energy security due to its wide adaptation. The objectives of this research were to study some genetic parameters of sorghum agronomic traits grown under acid soil conditions and to select aluminum-tolerant sorghum mutant lines.  The selection was conducted in an augmented design with 61 lines at the field research station of B2TP-BPPT Lampung. Genetic materials used were mutant lines developed throught gamma irradiation.  The results showed that there was significant differences among the mutant lines for  some agronomic characters observed.  The heritability estimates for agronomic characters were classified as intermediate to high.  Selection based on seed weight and biomass production resulted three best sorghum mutan lines i.e ZH30-29-07, ZH30-30-07, and ZH30-35-07.  These lines showed the best yield and biomass production under acid soil condition.   Key words:  sorghum, acid soil, heritability, selection

Pengembangan Kriteria Seleksi pada Pisang (Musa sp.) Berdasarkan Analisis Lintas

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 33, No 3 (2005): Jurnal Agronomi Iindonesia
Publisher : Bogor Agricultural University

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Abstract

This research was aimed to develop selection criteria based on correlation and path coefficient analysis.  The experiment was carried out at CETROFS (Center for Tropical Fruit Studies) field station of Bogor Agricultural University, Tajur.  The result showed that plant height, pseudostem diameter, number of leaves, weeks to harvest,  finger number per plant, and mean of  finger weight were positively correlated with bunch weight. Pseudostem diameter,  number of leaves, finger number per plant have strong direct effect on bunch weight,  with path coefficient 0.321, 0.264, 0.297, and 0.722, subsequently.  However, the plant height showed strong indirect effect on bunch weight  through  pseudostem diameter. These characters  are useful as a selection criteria for developing new variety of banana  with higher bunch weight   Key words: path coefficient, selection criteria, banana