I Gede Putu Wirawan
Central Laboratory for Genetic Resources and Molecular Biology, Udayana University, Bali, Indonesia

Published : 27 Documents
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Organogenic Regeneration of Transgenic Plant by Agrobacteriummediated DNA transformation of Citrus Wirawan, I Gede Putu; Subandiyah, Siti; Ngurah Suprapta, Dewa; Arya, Nyoman; Supartana, Putu; Sudana, Made
Journal of Bioscience and Biotechnology Vol. 1, No. 1 Januari 2007
Publisher : Journal of Bioscience and Biotechnology

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Internodal stem segment from citrus seedling were cultured and inoculatedwith Agrobacterium tumefaciens harboring binary Ti plasmid vector that containedthe genes for detecable marker ß-glucuronidase (GUS) and the selectable markerNptII. The result of this study show that shoots can be regenerated in media with 100?g/ml kanamycin and about 10 % of them were GUS+ shoots. Some of the GUS+shoots were confirmed by polymerase chain reaction (PCR) analysis for GUS genedetection in transformed shoots.
ANTIOXIDANT CAPACITY OF FRANGIPANI (Plumeria alba) POWDER EXTRACT Wrasiati, Luh Putu; Wirawan, I Gede Putu; Agus Bagiada, Nyoman; Mantik Astawa, I Nyoman
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 2 Mei 2011
Publisher : Udayana University

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This research aimed to identify the antioxidant capacity, vitamin C (ascorbic acid), and total phenolic compounds of frangipani flower powder. The powder was extracted using ethanol, methanol, acetic acid, and water (aquadest). Antioxidant capacity of each extract were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging method, vitamin C were determined using 2,4 Dinitrophenylhydrazine, and total phenolic compounds were determined using Folin Ciocalteu reagent. All of parameters were measured by spectrofotometer. The result shows that the highest value of antioxidant capacity was ethanolic extract (18.19%) and the lowest value was acetic acid extract (12.74%). The highest value of vitamin C was aqueous extract (3.49 mg/100g) and the lowest value was acetic acid extract (3.02 mg/100g). The highest value of total phenolic content was aqueous extract (25.49 mg GAE/g) and the lowest value was acetic acid extract (22.74 mg GAE/g). In conclusion, the higher antioxidant capacity was not always followed by the higher of vitamin C and total phenolic compounds.
POTENTIAL EFFECT OF MACRO ALGA Caulerpa sp. AND Gracilaria sp. EXTRACT LOWERING MALONDIALDEHYDE LEVEL OF WISTAR RATS FED HIGH CHOLESTEROL DIET Julyasih, Ketut Srie Marhaeni; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2017.v05.i01.p06

Abstract

Seaweed has potential nutrient content such as carotenoids, vitamins, fatty acids, carbohydrates, minerals, and other essential substances. Carotenoids have important biological functions as an antioxidant, and immunostimulatory which can prevent the disease, anti-inflammatory, anti-stress, anti-aging, and protect the skin from the harmful effects of ultraviolet radiation. Seaweed generally consumed as a vegetable by people in Bali, known as the local name Bulung Boni (Caulerpa spp.) and Bulung Sangu (Gracilaria spp.).. So far there has been no report or results of research on the effects of extract ethanol of Bulung Boni (Caulerpa sp.) and Bulung Sangu (Gracilaria sp.) as an antioxidant that can prevent lipid peroxidation which can be seen in decreased level of MDA in liver tissue or blood plasma. Therefore it is necessary to determine of plasmaMDA level of Wistar rat after fed high cholesterol diet treated with extract ethanol of Caulerpa sp. and Gracillaria sp. This experimental study used completely randomized design. Research using total of 24 Wistar rats divided into six sample groups of equal size, all fed with a diet high in cholesterol especially in negative control. The study consisted of negative control group (standard diet), positive control group (high cholesterol diet), high-cholesterol diet with Caulerpa sp. extract dose of 20 mg and 60 mg/100 g, high cholesterol diet with Gracilaria sp. extract dose of 20 mg and 60 mg/100 g body weight rat per day.The study resulted that rats fed high cholesterol diet with treated extract ethanol Caulerpa sp. and Gracilaria sp. with a dose of 20 mg and 60 mg per 100 g body weight rat / day had plasma MDA level significantly lower (p <0.05) compared with rats fed high cholesterol diet without treated with extract of Caulerpa sp. and Gracilaria sp.
IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI IN RHIZOSPHERE SOIL OF SEVERAL GRASS SPECIES AND CACAO (Theobroma cacao L.) BASED ON ITS SPORE MORPHOLOGICAL CHARACTERISTICS Sintya Dewi, Ni Kadek; Susrama, I Gede Ketut; Sritamin, Made; Adnyana, Made; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Arbuscular mycorrhizal fungi live in a symbiotic mutualism with plant roots and helps plant absorbnutrients and also able to live in various places. The study aimed to identify arbuscular mycorrhizal fungiin rizhosphere of several grass and cacao through microscopic method based on morphologicalcharacteristics and was conducted from December 2013 to March 2014. The methods used in this studywere spore isolation using wet sieving and root staining techniques. The results showed that arbuscularmycorrhizal fungi spores found in Imperata cylindrica L. were spores of Glomus (Glomales: Glomeaceae)with its vesicular and hyphae structure. The presence of spores and structures in Paspalum notatum wereAcaulospora (Glomales: Acaulosporaceae) and Gigaspora with its arbuscules, vesicules and hyphae structure.Spores and structures found in the Pennisetum purpureum were belong to genus of Glomus with hyphaeand arbuscular structure. Spores and structures of mycorrhizal fungi in Cyperus rotundus are spores of thegenus of Gigaspora (Glomales: Gigasporineae) and spores of Glomus with internal hyphae structure. Whilespores and mycorrhizal structures in Cacao were found two types of spore belong to genus of Glomus withhyphae and vesicular structures.
Isolasi dan Identifikasi Agrobacterium tumefaciens dari Tanaman Wortel (Daucus carota L.) MANALU, YOLANDA HASSIAN; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens from  Carrots (Daucus carota L.) Agrobacterium tumefaciens is a plant pathogenic bacterium that is widely used as a vector for inserting foreign genes into a plant chromosome to produce a transgenic plant. This bacterium cause a disease namely crown gall in dicotyledonous plants. This study is aim to isolate A. tumefaciens from local carrot (Daucus carota L.) by using some method such as selection media, characterization of the bacteria, and confirm by Koch’s postulates. The result of this study determined that carrot’s root taken from Bedugul area which showed crown gall symptom, an A. tumefaciens due to its characteristics e.g. shape of colonies, color, and growth of the bacteria in AB minimal medium that was a specific for A. tumefaciens.  By using the Koch’s postulates test showed that the isolated caused convex gall on the surface of carrot slice. DNA of isolated bacterium successfully electroforeted through agarose gel electrophoresis.  These results showed that the bacteria associated with carrot was A. tumefaciens. Keywords : A. tumefaciens, AB medium, Carrot, LB medium, Crown gall
Isolasi dan Identifikasi Agrobacterium Tumefaciens pada Tanaman Mawar (Rosa sp.). SILITONGA, NADIAH; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens At Plant roses (Rosa sp.). Agrobacterium tumefaciens caused crown gall tumor in many of dicotyledonous plants. The purpose of this study tried to isolate and identify the A. tumefaciens from rose plant. Various techniques were used such as selection media, colonies shape and color, Kochs postulate test, DNA isolation and agarose gel elektroforesis. The results of this study showed that A. tumefaciens can be isolated through culturing in LB medium and selection AB medium that was specific for A. tumefaciens. The isolate caused crown gall tumor on carrot slice 3 weeks after inoculation by using a modified Koch’s postulate test. The characteristics of colony formed in this research are round shaped, cream coloured with pink tint, smooth edge, and convex elevation. DNA isolation and its running in agarose gel electroforesis showed positive result.   Key words : Agrobacterium tumefaciens, AB medium and Agarose gel electroforesis.
Uji Efektivitas Beberapa Konsentrasi Ekstrak Daun Kirinyuh (Chromolaena odorata L.) Terhadap Perkembangan Ulat Krop Kubis (Crocidolomia pavonana F.) WIJAYA, I NYOMAN; WIRAWAN, I GEDE PUTU; ADIARTAYASA, WAYAN
Agrotrop : Journal on Agriculture Science Vol 8 No 1 (2018)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2018.v08.i01.p02

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Effectiveness Test of Several Concentrations of Kirinyuh Leaf Extract (Chromolaena odorata L.) Toward Development of Cabbage Crop Caterpillar (Crocidolomia pavonana F.). This research was conducted at the Laboratory of Plant Pests and Diseases of the Faculty of Agriculture, Udayana University from April 2018 to August 2018 to test the extract of kirinyuh leaves (Chromolaena odorata) on the development of biology of cabbage crop caterpillar (Crocidolomia pavonana). The completely randomized design (CRD) was used in this study, with four types of treatment of leaf extract concentration of 10%, 20%, 30%, 40%, and control. Each treatment was repeated 10 times. Observations were completed every day of the larval stage until the larvae do not carry out any activity anymore (dead), or until they become imago. The results showed that (1) the leaf extract of C. odorata plant which was used as research material had the potential to suppress the biological development of C. pavonanadi larvae in the laboratory, (2) the application of leaf extract concentration of 10% and 20% had an influence on the development of larvae which had developed slow compared to concentrations of 30% and 40%, where larvae experience rapid development of only 9 observations and (3) the most effective test leaf extract treatment was the treatment of leaf extracts of C. odorata concentrations of 40% and 30% followed by leaf extract concentrations of 20% and 10%.
Isolasi dan Identifikasi Fungi Mikoriza Arbuskular (Fma) secara Mikroskopis pada Rhizosfer Tanaman Jeruk (Citrus sp.) di Desa Kerta, Kecamatan Payangan, Kabupaten Gianyar SUAMBA, I WAYAN; WIRAWAN, I GEDE PUTU; ADIARTAYASA, WAYAN
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Isolation and Identification of Arbuscular Mycorrhizal Fungi (AMF) by Microscopic (AMF) in Rhizosphere of Citrus Plant (Citrus sp.) in Kerta Village, Payangan District, Gianyar Regency Arbuscular Mycorrhizal Fungi (AMF) is a group of organisms from fungi that describes a form of mutualism between symbiotic fungi and plant roots. AMF has great potential as a biological fertilizer because the microorganism which has a very important role in facilitating the absorption of nutrients in the soil to increase plant growth, in addition AMF also function as a biological barrier against pathogen that infect the roots, increasing the availability of water for plants to grow and improve hormone boosters. The purpose of the study was to determine the types of AMF that exist in the rhizosphere of citrus plant and determine the presence of infection by the AMF in the citrus plant roots in the  village of Kerta. The results of the isolation and identification of AMF spores in the rhizosphere of citrus plants in the village of Kerta found 14 different types of AMF spores. This study found 7 type of Glomus, 5 types of Gigaspora and 2 types of Acaulospora. Those types were determined by using the characteristic of their spores. Observations on root finding that the AMF  infection in the form vesicles and arbuscular structures. Keywords : Arbuscular Mycorrhizal Fungi, Glomus, Gigaspora, Acaulospora, Vesicles and Arbuscular.
Identifikasi Fungi Mikoriza Arbuskular Secara Mikroskopis pada Rhizosfer Tanaman Alang-Alang (Imperata Cylindrica L.) di Desa Sanur Kaja NAINGGOLAN, ROMAULI THERESIA; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Identification of Mikoriza Arbuskular Fungi in Microscopic the Rhizosphere of Reed (Imperatacylindric L.) in Sanur Kaja Village.   Mycorrhiza is a form of symbiosis between the fungus with a high level of plant (vascular plants, Tracheophyta), especially on the rooting. There is also a fungus with the other symbiotes, but the term mycorrhiza are typically for symbiotic plant roots which infect by fungus. This research aims to know the diversity of the genus or species of  mycorrhiza fungi arbuskular on the rhizosfer plant reeds in the village of Sanur Kaja, and to know whether or not there was colonization of mycorrhiza fungi arbuskular on the net root crops the reeds in the village of Sanur Kaja. The results of this research show that the symbiotes spores in the rhizosfer plant reeds is the genus Glomus multicaule spores, Glomus ambisporum, Acaulospora foveata, Gigaspora gigantae. Analysis on the plant roots mycorrhiza colonization reed showed a spherical structure called vesikular, while arbuskular is a structure on the hypha branches that resemble haustorium. (forming the pattern dikotom).   Key words: Acaulospora sp, Gigaspora sp, Glomus sp.
Identifikasi Mikoriza Abuskula Secara Mikroskopis pada Rhizosfer Beberapa Jenis Rumput-rumputan dan Tanaman Kakao (Theobroma cacao L.) DEWI, NI KADEK SINTYA; WIRAWAN, I GEDE PUTU; SRITAMIN, MADE
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Identification of Arbuscular Mycorrhiza through Microscopis Methode in Rhizosfer of Several Grass and Cacao ( Theobroma cacao L.) Arbuscular mycorrhiza fungi that live in a symbiotic mutualism with plant roots and helps the absorption of plant nutrients, and also able to live in various places. The study aimed to identifiy arbuscular mycorrhiza through microscopic methode in rhizosfer of several grass and cacao was conducted from December 2013 to March 2014. The method used in this study were spore isolation by wet sieving and root staining techniques. The results showed that arbuscular mycorrhiza spores and structures found in Imperata cylindrica L. are 2 types spores of genus Glomus (Glomales: Glomeaceae) with vesicular and hyphae structures. The presence of spores and structures in Paspalum notatum are Acaulospora (Glomales: Acaulosporaceae) and genus Gigaspora (Glomales: Gigasporineae) with arbuscular, vesicular and hyphae structure. Spores and structures of mycorrhiza found in the Pennisetum purpureum are 3 types of the genus Glomus with hyphae and arbuscular structure. Spores and structures mycorrhiza in the Cyperus rotundus are 1 type spore of the genus Gigaspora and 2 types of spores Glomus with internal hyphae structure. While spores and mycorrhiza structures in the cacao are 2 types spore of the genus Glomus with hyphae and vesicular structures.   Keywords: Glomus, Gigaspora, Acaulospora, Arbuscular Mycorrhiza Structure