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The Correlation between Calcium Serum and Calcium Urine Level with the Blood Pressure in Preeclampsia

Indonesian Journal of Obstetrics and Gynecology Vol. 36. No 1. January 2012
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

AbstractObjective: To analyze the differences of calcium serum and calcium urine level in preeclampsia and normal pregnancy, and to analyze the correlation between calcium serum and calcium urine level with blood pressure.Method: This study is a cross sectional study with 44 women with preeclampsia and 45 women with normal pregnancies, that meet our inclusion criteria. The samples were obtained from Dr. Hasan Sadikin Hospital and six satellite hospitals from June to September 2011. The comparison of mean calcium serum and calcium urine level of the preeclampsia group was calculated using Mann-Whitney test, and the correlation between calcium serum and calcium urine level and preeclampsia were calculated using Rank Spearman correlation test.Result: The result of the characteristic test in two groups of study shows that both groups are homogenic and comparable. The mean of calcium serum level in women with preeclampsia (7.97 mg/dl) is lower than in normal pregnancy (8.82 mg/dl) with p<0.0001 and the mean of calcium urine level in women with preeclampsia (1.725 mg/dl) is lower than normal pregnancy (2.809 mg/dl) with p<0.0001. There is a negative correlation between calcium serum level (rs = –0,62; p<0,001) and calcium urine level (rs= –0,68; p<0,001) with systolic pressure in preeclampsia. There is also a negative correlation between calcium serum level (rs = –0,65; p<0,001) and calcium urine level (rs = –0,68; p<0,001) with diastolic pressure in preeclampsia.Conclusion: There is a negative correlation between calcium serum level and calcium urine level with systolic and diastolic pressure in preeclampsia, meaning that the lower the calcium serum and calcium urine level is, the higher systolic and diastolic pressure.[Indones J Obstet Gynecol 2012; 36-1:3-7]Keywords: blood pressure, calcium blood level, calcium urine level, preeclampsia

Isolation and Characterization Cytotoxic Compounds Caffeine and Chlorogenic Acid Seeds of Lampung Coffee Robusta

Jurnal Medika Planta Vol 1, No 4 (2011)
Publisher : Jurnal Medika Planta

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Abstract

Therapeutic use of natural compounds as the active ingredient is a breakthrough anticancer ingredient in pharmaceuticals and medicine. Caffeine and chlorogenic acid have isolated from coffee, there are cytotoxic and contain high antioxidants.Objective: to determine cytotoxic and antioxidants effect of caffeine and chlorogenic acid in coffee rubusta Lampung. Methods: Research have carried out by several steps including: separation and purification via extraction and fermentation of robusta coffee beans, maceration using methanol solvent, fractionation using n-hexane and water, the purification is done by using various combinations of chromatographic techniques using normal phase and reversed-phase column on an open and press column, the purity of the active compound analysis by performed the method of thin layer chromatography (TLC) and reversed and normal phase high performance liquid chromatography method (HPLC / HPLC), toxicity tests carried out on brine shrimp (A. salina), and the antioxidant test using the method trapping of free radicals 1.1 Diphenyl-2-picrylhidrazyl (DPPH). Result: Isolates of caffeine has cytotoxic activity against larvae of shrimp with a value of 100% mortality at 1000 ppm, while the smallest 60% mortality at concentrations of 10 ppm with IC50 values for caffeine obtained at 21.41 ppm. Whereas chlorogenic acid has a cytotoxic activity with a value of 100% mortality at concentrations of 100, 300, and 1000 ppm and the smallest 70% mortality at concentrations of 10 ppm with IC50 values of 5.86 ppm. Conclusion: Both compounds have a cytotoxic activity with LC50 values of less than 1000 ppm, and chlorogenic acid had higher antioxidant activity compared to caffeine.

Polimorfisme C1167T Gen Reseptor Tipe II Transforming Growth Factor-â, Kadar Soluble Endoglin, dan Vascular Cell Adhesion Molecule-1 pada Preeklamsia

Majalah Kedokteran Bandung Vol 42, No 3 (2010)
Publisher : Fakultas Kedokteran, Universitas Padjadjaran

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Abstract

Transforming growth factor-â (TGF-â) diduga berperan pada preeklamsia. Reseptor TGF-â tipe II (TâR-II) dihasilkan dari transkripsi gen TGF-â receptor type II (TGFBR2). Polimorfisme gen TGFBR2 pada basa C1167T dapat menyebabkan hipoksia yang menginduksi iskemia serta meningkatkan produksi solubel endoglin (sEng) dan vascular cell adhesion molecule-1 (VCAM-1). Tujuan penelitian ini untuk mengetahui korelasi polimorfisme gen TGFBR2 pada basa C1167T dengan kadar sEng dan VCAM-1 ibu preeklamsia. Subjek adalah ibu preeklamsia usia kehamilan 28–42 minggu dan kehamilan normal sebagai kontrol, masing-masing 120 orang. Penelitian dilakukan di Rumah Sakit Hasan Sadikin, Bandung, September 2008–Mei 2009. Sampel berupa darah vena, pemeriksaan polimorfisme dilakukan dengan DNA Wizard® genomic DNA purification, kadar sEng dan VCAM-1 dengan imunoesai. Hasil penelitian menunjukkan polimorfisme CT pada kelompok preeklamsia 92 (76,7%) dan kontrol 70 (58,3%) {p<0,001; OR (95%CI): 2,35 (1,30–4,26)}. Kadar sEng (ng/mL) 12,46 berbanding 10,29 pada kelompok kontrol {p<0,001; OR (95%CI): 3,71 (2,11–6,57)}. Kadar VCAM-1 berbeda bermakna, yaitu 1.218,43 berbanding 705,59 {(p<0,001; OR (95%CI): 7,56 (4,11–14,0)}. Disimpulkan terdapat perbedaan proporsi dan korelasi polimorfisme C1167T gen TGFBR2, kadar sEng, dan VCAM-1 antara preeklamsia dan kehamilan normal. [MKB. 2010;42(3):115-22].Kata kunci: Polimorfisme gen TGFBR2, preeklamsia, sEng, VCAM-1C1167T Type II Transforming Growth Factor-â Receptor Gene Polymorphism, Soluble Endoglin and Vascular Cell Adhesion Molecule-1Levels in PreeclampsiaTransforming growth factor-â (TGF-â) plays a role in preeclampsia. TGF-â receptor type II (TâR-II) is produced from the transcription of the type II TGF-â receptor gene (TGFBR2). Polymorphism of TGFBR2 gene on the base C1167T could cause hipoxia that induces ischaemia and product soluble endoglin (sEng) and vascular cell adhesion molecule-1 (VCAM-1). The aim was to find out the association of C1167T type II TGF-â receptor gene polymorphism with sEng and VCAM-1 levels in preeclampsia. The study was done at Hasan Sadikin Hospital, Bandung, September 2008–May 2009. Indicates that C1167T polymorphism events were found in the preeclampsia that were 92(76.7%) of 120 cases and 70 (58.3%) control of 120 normal pregnancies with the difference in the appearance polymorphism which means p<0.001 OR (95%CI):2,35 (1.30–4.26). There was a difference between sEng (ng/μL) 12.46 for preeclampsia and 10.29 for the control group p<0.001 OR (95%CI): 3.71 (2.11–6.57). There was also a difference between VCAM-1 (ng/μL) 1,218.43 for the preeclampsia and 705.59 for the control group {p<0.001 OR (95%CI): 7.56 (4.11–14.0)}. There was a result that in preeclamptic patient having polymorphism sEng level was 14.19 ng/mL and VCAM-1 level is 961,85 ng/mL. It is concluded that there are difference proportion and association of C1167T type II TGF-â receptor gene polymorphism with sEng and VCAM-1 levels between preeclampsia and normal pregnancy patients. [MKB. 2010;42(3):115-22].Key words: Preeclampsia, sEng, TGFBR2 gene polymorphism, VCAM-1

Uji Fungsional dan Karakteristik Sel Punca Hematopoetik Hasil Isolasi dari Darah Tali Pusat Manusia Menggunakan Metode Modifikasi Unpad- Aster

Majalah Kedokteran Bandung Vol 43, No 4 (2011)
Publisher : Fakultas Kedokteran, Universitas Padjadjaran

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Abstract

Metode isolasi sel-sel mononuklear/mononuclear cells (MNCs) dari darah tali pusat (DTP) manusia secara konvensional menghasilkan tingkat kontaminasi sel eritrosit yang sangat tinggi. Oleh karena itu, tujuan penelitian ini adalah menilai perbedaan viabilitas dan kontaminasi sel eritrosit dalam populasi MNC DTP pada modifikasimetode isolasi yang kami kembangkan. Penelitian ini juga bertujuan untuk menguji fungsi dan karakteristik populasi MNCs dari DTP manusia sebagai dasar pembangunan bank darah tali pusat di Indonesia. Penelitian dilakukan di Departemen Obstetri dan Ginekologi RS Dr. Hasan Sadikin Bandung periode Januari–Oktober 2010. Isolasi MNCs dengan metode modifikasi (dinamakan modifikasi Unpad-Aster) yang menghasilkan 5,1x106 sel/mL memiliki tingkat kontaminasi sel eritrosit yang lebih rendah dibandingkan dengan metode konvensional. Morfologi sel yang dibiakkan dalam medium unrestricted somatic stem cells (USSCs) tampak seperti sel-sel yang adheren (menempel di dasar), berbentuk sel spindle, dengan cluster of differentiation-90 (CD-90) (antigen leukosit) dan cluster of differentiation-105 (CD-105) yang positif serta dapat berdiferensiasi menjadi sel neuron dan adiposit; sedangkan morfologi untuk cord blood-derived multipotent progenitor cells (CB-MPCs) tampak seperti sel-sel fibroblas dengan cluster of differentiation-45 (CD-45) (antigen hematopoetik) yang positif serta dapat berdiferensiasi menjadi sel neuron. Disimpulkan bahwa metode modifikasi Unpad-Aster memberikan tingkat kontaminasi eritrosit yang lebih rendah dibandingkan dengan metode konvensional. Sel mononuklear yang berasal dari darah tali pusat ini dapat berdiferensiasi menjadi sel-sel neuron dan adiposit. [MKB. 2011;43(4):171–7].Kata kunci: Darah tali pusat (DTP), diferensiasi, karakterisasi, modifikasi Unpad-Aster, sel mononuklearFunctional Test and Characteristic of Hematopoietic Stem Cells Derived from Human Umbilical Cord Blood Using Unpad-Aster’s Modified MethodThe conventional method of mononuclear cells (MNCs) isolation from human umbilical cord blood (UCB) yielded high erythrocyte contamination level. Therefore, the aim of this study was to assess the differences of cell viability and erythrocyte contamination on the population of UCB MNCs in our modified isolation method. This study was also aimed to test the function and characteristic of human MNCs derived from UCB as the basis for the development of UCB banking in Indonesia. The study was conducted in Department of Obstetry and Ginecology >RS Dr. Hasan Sadikin Bandung in period of January–October 2010. The modified isolation method (namely Unpad Aster’s modification) yielded 5.1x106 MNC cell/mL has lower erythrocyte contamination level than conventional method. The morphology of MNCs cultured in unrestricted somatic stem cells (USSCs) medium looked like adhered cells (attached at the surface of culture flask), spindle-shaped cells with positive luster of ifferentiation-90 (CD-90) (leukocyte antigen) and cluster of differentiation-105 (CD-105) and could differentiate into neuronal cells and adipocytes. While the morphology of cord blood-derived multipotent progenitor cells (CB-MPCs) looked like fibroblast cells with positive cluster of differentiation-45 (CD-45) (antigen hematopoietic) and could differentiate into neuronal cells. In conclusions, the Unpad-Aster’s modified isolation method gives lower level of erythrocyte contamination compared with conventional method. Mononuclear cells derived from UCB could differentiate into neuronal cells and adipocytes. [MKB. 2011;43(4):171–7].Key words: Characteristic, differentiation, mononuclear cells (MNCs), umbilical cord blood, Unpad-Aster modification

KORELASI KUNJUNGAN ANTENATAL CARE BURUH PABRIK DENGAN HASIL LUARAN BAYI DI KABUPATEN KUDUS

JURNAL ILMU KEPERAWATAN DAN KEBIDANAN Vol 4, No 2 (2013): JURNAL ILMU KEPERAWATAN DAN KEBIDANAN
Publisher : STIKES Muhammadiyah Kudus

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Abstract

ABSTRAK Latar Belakang : Hasil luaran bayi merupakan hasil dari kehamilan yang dapat berupa hasil luaran menguntungkan dan tidak menguntungkan. Kunjungan Antenatal care (ANC) yang adekuat dan berkualitas dipercaya dapat mencegah terjadinya hasil luaran yang tidak menguntungkan. Tujuan : penelitian ini adalah untuk menganalisis kunjungan ANC  buruh pabrik dengan hasil luaran bayi yang tidak menguntungkan. Penelitian ini dilakukan di Kabupaten Kudus dari bulan Februari-April 2013 dengan rancangan penelitian potong lintang dan didapatkan 92 responden. Metode :Analisis dilakukan secara bivariabel dengan menggunakan uji Rank Spearman, Hasil penelitian : menunjukkan ada korelasi antara kunjungan ANC dengan hasil luaran bayi, untuk jumlah kunjungan (r=0,206., p=0,049) dan waktu kunjungan (r=-0,455., p=0,000). Simpulan : dalam penelitian ini adalah jumlah dan waktu kunjungan ANC berkorelasi dengan hasil luaran bayi, semakin banyak jumlah kunjungan ANC semakin baik hasil luaran bayi dan semakin terlambat waktu kunjungan ANC semakin jelek hasil luaran bayi. Kata kunci : Buruh pabrik rokok, hasil luaran bayi, Kunjungan ANC.

Peran Siklooksigenase dalam Pertumbuhan Kanker Leher Rahim

Majalah Kedokteran Bandung Vol 42, No 4 (2010)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Ekspresi dan penghambat selektif siklooksigenase-2 (COX-2), seperti selekoksib yang telah dipakai luas sebagai antiinflamasi, diketahui berperan pada kanker dengan menghambat proliferasi dan pertumbuhan tumor serta meningkatkan apoptosis. Tujuan penelitian ini untuk mengetahui pengaruh penghambat COX-2 selektif dan peran COX-2 pada pertumbuhan tumor. Metode penelitian adalah uji eksperimental dengan pretest-posttest control group design yang dilakukan di Rumah Sakit Hasan Sadikin Bandung, November 2007–Oktober 2008. Dua puluh pasien diberi penghambat selektif COX-2 serta kemoradiasi dan 21 pasien mendapat kemoradiasi saja. Dilakukan pemeriksaan COX-2, Ki-67, kaspase-3 secara imunohistokimia, serta ukuran serviks dengan USG transabdominal, praradiasi dan pascaradiasi. Data dianalisis dengan tes Wilcoxon dan korelasi Pearson. Penghambat COX-2 selektif mengakibatkan penurunan sangat bermakna tingkat ekspresi COX-2, yaitu 10% pada kelompok kontrol dan 42% pada kelompok perlakuan (p=0,001), serta ekspresi Ki-67 sebagai penanda proliferasi sebanyak 48% dan -3% pada kelompok kontrol (p=0,007). Tingkat ekspresi kaspase-3 sebagai penanda apoptosis meningkat dengan pemberian penghambat COX-2 selektif sebesar -59% dan 16% pada kelompok kontrol (p<0,001). Penghambat COX-2 selektif juga menyebabkan bertambahnya pengecilan tumor, yaitu: 88% dibandingkan dengan 83% pada kelompok kontrol (p<0,001). Simpulan, COX-2 berperan dalam kanker leher rahim dan penghambat COX-2 selektif menurunkan proliferasi dan meningkatkan apoptosis, sehingga terjadi pengecilan tumor. [MKB. 2010;42(4):169–74].Kata kunci: Apoptosis, COX-2, kanker leher rahim, penghambat COX2 selektif, pertumbuhan tumorRole of Cyclooxygenase on Cervical Cancer GrowthCyclooxygenase-2 (COX-2) expression and selective COX-2 inhibitor such as celecoxib, which widely used as antiinflamatory drug, is known to have role in cancer by reducing proliferation and growth of tumor cells, and increasing apoptosis. The research aims were to investigate the effect of selective COX-2 inhibitor and role of COX-2 on tumor growth. This was an experimental study with pretest-posttest control group design. This study was done at Hasan Sadikin Hospital Bandung, November 2007–October 2008. Twenty patients received selective COX-2 inhibitor and chemoradiation, whereas 21 patients were treated by chemoradiation only, as control group. COX-2, Ki-67, and caspase-3 expression was analyzed by immunohistochemistry. Cervical size was measured by transabdominal ultrasonograpy. All variables obtained before and after external chemoradiation. Data were analyzed using Wilcoxon and Pearsons correlation test. Selective COX-2 inhibitor significantly reduced COX-2 expression, 10% in control group and 42% in treated group (p=0.001) as well as Ki-67 expression as proliferation marker, -3% in the control group and 48% in the treated group (p=0.007). Caspase-3 expression as marker of apoptosis was increased after selective COX-2 inhibitor treatment, 59% whereas only 16% in the control group (p<0.001). In addition, selective COX-2 inhibitor enhanced tumor reduction, 88%versus 83% in control group (p<0.001). In conclusion, COX-2 plays role in uterine cervical cancer and selective COX-2 inhibitor reduced proliferation and increased apoptosis which leads to reduction in tumor size. [MKB. 2010;42(4):169–74].Key words: Apoptosis, cervical cancer, COX-2, proliferation, selective inhibitor COX2, tumor growth DOI: http://dx.doi.org/10.15395/mkb.v42n4.31

A multivariate analysis of risk factors influencing the cesarean section rate in two university hospitals in Leiden (the Netherlands) and Bandung (Indonesia)

Medical Journal of Indonesia Vol 7, No 1 (1998): January-March
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

[no abstract available]

Soluble Endoglin Serum Level is Higher in Preeclampsia Compared to Molar and Normal Pregnancy

Indonesian Journal of Obstetrics and Gynecology Vol. 2. No. 2. April 2014
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Objective: To analyze th e differences of maternal serum soluble endoglin level in pr eeclampsia, molar pregnan cy, and normal pregnancy, and to analyze th e correlation between maternal serum soluble endoglin level with gestational age.Method: This is a cros s-sectional compara tive analytic study involving 18 preeclamps ia cases, 18 molar pr egnan cies, and 18 normal pregnancies. The sample were obtained from Dr. Hasan Sad ikin hospital and six satellite hospitals from January until Mar ch 2013. The comparison of mean seng se rum level of th e preeclampsia,molar pregnancy, and normal pregnancy gro up was calculated using Kruskal Wallis, and the corr elation we re calculated using Rank Spearman.Result: The mean level of seng se rum in pr eeclamp sia gro up was higher (168.79 ngjml) than in molar pr egnan cy (43.47 ngjml) and normal pregnancy (32.38 ngjml). Ther e is no significant difference of se rum seng level be tween molar and norm al pregnan cy, with p value of 0.393 (p>0.05). There is significant differ ences of seng serum level between pr eeclampsia gro up and molar pr egnancy (p=O.OOO), but no significant differ ences between molar and normal pregnancy, p value=0.393 (p >0.05). Ther e is positive correlati on between seng se rum level of norm al pregnancy with gestational age (rs=0 .647 ; p<0.001).Conclusion: Mat ern al se rum seng level in preeclampsia is higher than th e level of which in molar pre gnan cy and norm al pregnancy.Keywords: molar pregnancy, norm al pr egnancy, pr eeclamp sia, soluble endoglin level

EVALUASI PENGARUH LAMANYA PEMBERIAN ASI SAJA TERHADAP PERTUMBUHAN ANAK Suatu Studi di Kecamatan Ledo, Kabupaten Bengkayang, Provinsi Kalimantan Barat

Majalah Kedokteran Bandung Vol 41, No 1 (2009)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Air susu ibu (ASI) merupakan makanan yang kaya akan gizi dan sangat penting untuk pertumbuhan anak. Terganggunya pertumbuhan anak diawali dengan kekurangan gizi yang dapat diatasi dengan memberikan ASI saja sejak lahir. Tujuan penelitian ini untuk mengetahui pengaruh lamanya pemberian ASI saja pada pertumbuhan anak. Faktor yang dilihat adalah berat badan dan tinggi badan anak. Penelitian ini merupakan penelitian kohort retrospektif yaitu mencari efek yang muncul pada balita usia antara 12 sampai 36 bulan yang ketika bayinya menggunakan ASI saja di wilayah Kecamatan Ledo. Jumlah subjek yang diteliti sebanyak 101 anak. Sampel diambil dengan teknik cluster berdasarkan kriteria inklusi. Analisis statistik memakai analisis varians, uji Mann-Whitney, Kruskal-Wallis, dan korelasi regresi. Hasil penelitian ini menunjukkan bahwa lamanya pemberian ASI saja mempengaruhi pertumbuhan anak berdasarkan persen terhadap median Berat Badan (BB)/Usia (U) dan Berat Badan (BB)/Tinggi Badan (TB) (p<0,001); tidak tampak pengaruh lamanya pemberian ASI dengan pertumbuhan anak berdasarkan persen terhadap median TB/U baku rujukan WHO-NCHS (p>0,05). Kesimpulan pada penelitian ini adalah lamanya pemberian ASI saja berpengaruh positif terhadap pertumbuhan yang diukur berdasarkan persen terhadap median BB/U dan BB/TBbaku rujukan WHO-NCHS.Kata kunci: Lamanya pemberian ASI, pertumbuhan anak, antropometriEVALUATION THE IMPACT BREASTFEEDING PERIOD OF TIME TO THE CHILD GROWTH: A Study In Ledo Subdistrict, Bengkayang Regency, West Kalimantan Province Breastfeeding is rich food nutrient and it is very important for the child growth. Neonatal growth disorders, it is initially revealed from the beginning by a lack of nutrient. The lack of nutrient can be minimized by giving breastfeeding since the first living without any additional food. The objective of this study was to know the impact of length duration influenced of breastfeeding to the child growth that was seen from the childs body weight and body height. This study designed as a retrospective cohort looking for an effect of weight and height growth of subject child age between 12 to 36 months used to have only breastfeeding in Ledo. The study carried out to 101 children. The samples collected by cluster technique, the inclusion criteria had been determined. Statistical analysis used, varians, Mann-Whitney, Kruskal-Wallis, and analysis regression.The result of study, it was shown that the duration of breastfeeding affect of child growth significantly different; the percentage of median body weight to age vs body weight to body height referred to WHO-NCHS standard (p<0.001); but there were no any correlation on the percentage of median body height to age referred to WHO-NCHS standard (>0.05). Conclusion: duration of breastfeeding has positive effect to the growth which is measured based on the percentage of median body weight/age and body weight/body height referred to WHO-NCHS standard.Key words: Duration of breastfeeding, child growth, anthropometry DOI: http://dx.doi.org/10.15395/mkb.v41n1.181

Perbedaan antara Faktor Intrinsik dan Ekstrinsik pada Pasien Infeksi Nosokomial di Bagian Bedah dan Medikal RSUP. Dr. Hasan Sadikin Bandung

Majalah Kedokteran Bandung Vol 50, No 4 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Infeksi nosokomial merupakan satu masalah komplikasi di rumah sakit dan menjadi permasalahan penting bagi kesehatan publik di dunia. Kecenderungan pasien menderita infeksi nosokomial (HAIs) ditentukan oleh faktor intrinsik dan faktor ekstrinsik. Metode penelitian yang digunakan adalah potong lintang. Terdapat 287 pasien yang mengalami infeksi nosokomial yang disebabkan oleh Klebsiella pneumoniae di Bagian Bedah dan Medikal Rumah Sakit Dr. Hasan Sadikin Bandung (RSUP) selama periode Januari sampai Juni tahun 2015 yang telah memenuhi kriteria inklusi dan tidak termasuk eksklusi. Hasil penelitian menunjukkan perbedaan antara faktor intrinsik dan ekstrinsik pada pasien infeksi nosokomial yang disebabkan oleh klebsiella pneumoniae di bagian Bedah dan Medikal RSUP Dr. Hasan Sadikin Bandung dengan nilai p<0,05. Terdapat perbedaan kejadian resistensi terhadap karbapenem pada kasus infeksi nosokomial selain kadar Hb adalah tindakan medis untuk tindakan medis sedang mempunyai risiko 2,06 kali (IK 95%; 1,0–4,28 ), pada tindakan medis berat 3,03 kali (IK 95%; 1,21–7,61) bila dibanding dengan tindakan medis ringan. Terdapat perbedaan dengan ketidaksembuhan pada kasus infeksi nosokomial adalah kasus rawat medikal, leukosit >16.600, tindakan medis berat, dan keadaan kulit terbuka dengan OR masing masing 2,89; 2,09; 5,05; dan 1,88. Saran, untuk memberikan pelayanan yang prima dengan memperhatikan faktor intrinsik pasien baik usia, jenis kelamin, keadaan luka kulit dan status gizi, juga memperhatikan faktor ektrinsik berupa lamanya masa rawat, tempat pengambilan sampel, dan tindakan medik yang dilakukan. Kata kunci: Faktor intrinsik dan ekstrinsik, infeksi Klebsiella pneumoniae, kasus bedah dan medikal, nosokomialinfeksiDifference between Intrinsic and Extrinsic Factors of  Nosocomial Infection Patients in The Surgery and Medical Ward of Dr. Hasan Sadikin General Hospital BandungNosocomial infection or Hospital-Acquired Infection (HAI) occurs as a complication during hospitalization in hospitals and becomes an important global public health problem. The tendency of patients suffering from nosocomial infectionis determined by intrinsic and extrinsic factors. This was a cross-sectional study on 287 patients with nosocomial infection caused by Klebsiella pneumoniae at the Surgical and Medical wards of Dr. Hasan Sadikin General Hospital during the period January to June 2015 who met the inclusion and exclusion criteria. Results showed the difference in intrinsic and extrinsic factors in patients with nosocomial infections caused by Klebsiella (p<0.05). There was a difference in the resistance towards Carbanepem in nosocomial infections. Factors influencing this were Hb level and medical actions. Patients with intermediate medical procedures had 2.06 times higher risk (CI 95%; 1.0–4.28 ) while in those with complicated medical procedures, the risk was 3.03 times higher (CI 95%; 1.21–7.61) when compared to those receiving simple medical procedures. A difference was also seen in the failure to recover in nosocomial infection between the medical inpatient cases (leucocyte of >16,600), complicated medical procedure, and open-skin condition with ORs of 2.89; 2.09; 5.05; and 1.88, respectively. It is suggested to provide excelent services by paying atttention to the intrinsic factors of patients, i.e. age, gender, skin wound status, and nutrition status and the extrinsic factors, i.e. length of stay, sampling sites, and medical procedures performed.Key words: Intrinsic and extrinsic factors , Klebsiella pneumoniae infection, nosocomial infection, surgical andmedical cases