Ida Bagus Oka Winaya
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Deteksi Toxoplasma gondii pada Mencit yang Diinfeksi Inokulat Jantung dan Otak Ayam Buras Apsari, Ida Ayu Pasti; Winaya, Ida Bagus Oka; Swacita, Ida Bagus Ngurah
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : Buletin Veteriner Udayana

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Abstract

Toxoplasma gondii adalah parasit intraseluler obligat menyebabkan penyakittoksoplasmosis yang sudah tersebar di seluruh dunia. Mencit sangat peka terhadapToxoplasma gondii, sehingga dipilih sebagai hewan model untuk toksoplasmosis. Mencitsebagai hewan model dilakukan penelitian dengan menginfeksikan inokulat jantung danotak ayam buras. Tujuan penelitian untuk mendeteksi Toxoplasma gondii pada mencitsecara serologis dengan metode ELISA dan secara histopatologis dengan metodemikroskopis dan pengecatan Haematoxylin Eosin preparat histologi. Sejumlah 18 inokulatjantung dan 18 inokulat otak ayam buras berasal dari sembilan kabupaten di Bali,diinokulasi ke 72 ekor mencit. Hasil penelitian diperoleh 9 inokulat jantung positif pada 10ekor mencit dan 2 inokulat otak positif pada 3 ekor mencit, dengan titer antibodi serum 512– 1024 EU. Deteksi secara histopatologis pada organ hati, jantung, otak, ginjal dan paruterjadi degenerasi, peradangan dan perdarahan interstitialis. Tidak ditemukan sista padasemua organ mencit yang diperiksa.
Kadar Perasan Kunyit yang Efektif Memperbaiki Kerusakan Hati Mencit yang Dipicu Karbon Tetrachlorida (THE EFFICACY OF TURMERIC JUICE ON PATHOLOGICAL CHANGES OF THE MICE LIVER BY INDUCED CARBON TETRACHLORIDE) Kardena, I Made; Winaya, Ida Bagus Oka
Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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A study on effect of various concentrations of turmeric juice on pathological changes mice liver hasbeen carried out. As many as 25 mice were used in this study and they were divided into 5 groups, i.e.: groupK-: 0.2 ml vegetable oil without CCl4; group K+: CCl4 without turmeric extract; group T1: CCl4 with 10%turmeric extract; group T2: CCl4 with 20% turmeric extract and group T3: CCl4 with 30% turmeric extract.All mice, except the K- group, were treated with 0.2 ml CCl4 orally for 7 days. On day-8 to day-29, the CCl4treated mice were given various concentrations of turmeric extract, i.e.: 10%, 20% and 30%; whereas, the KandK+ groups were given drinking water adlibitum. On day-30, all mice were sacrificed and their livertissues were collected and then processed to histopathological staining with haematoxylin-eosin. Theresult showed a clear evidence to suggest that turmeric extract can induce a better recovery compared tonon-treated mice. The improvement of pathological condition was characterized by reduction of pathologicalchanges found in the liver cells of mice, such as: hidrophic degeneration. fatty degeneration, and necrosis.More importantly, treatment with 30% of turmeric juice induced a better recovery than those with 10%and 20%.
Kejadian Balantidiosis pada Babi Landrace (A CASE STUDY OF BALANTIDIOSIS IN LANDRACE SWINE ) Winaya, Ida Bagus Oka; Berata, I Ketut; Apsari, Ida Ayu Pasti
Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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Abstract

The aim of the study was to identify the incidence of balantidiosis in landrace pigs. A total of 60 pigswere examined at Faculty of veterinary medicine, Udayana University between January 2007 and January2008. Seven out of go pigs showed cahexia and diarrhoea . Macroscopic changes were observed, such as: thecolon was fully distended with gas and slight peritonitis,whereas microscopic examination revealed thepresence of Balantidium coli trophozoites and cysta within the intestinal mucosa. Additionally, enteritiskatarrhalis, slight hemorrhagis, erosin and pseudomembranous inflammation with lymphocytes andpolymorphonuclear cells were also noted.
STUDI HISTOPATOLOGI HATI TIKUS PUTIH (Rattus norvegicus) YANG DIBERI EKSTRAK ETANOL DAUN KEDONDONG (Spondias dulcis G.Forst) SECARA ORAL Adikara, I Putu Arya; Winaya, Ida Bagus Oka; Sudira, I Wayan
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : Buletin Veteriner Udayana

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Abstract

Tanaman kedondong sering dimanfaatkan oleh masyarakat sebagai obat alternatif  untuk mengobati berbagai macam penyakit. Sedangkan penelitian tentang toksisitas daun kedondong pada hati belum pernah dilakukan. Dalam penelitian ini tikus putih (Rattus norvegicus) dibagi secara acak menjadi lima kelompok, masing-masing kelompok berjumlah 5 ekor. Kelompok A sebagai kontrol (placebo) yang diberi aquades peroral; kelompok B diberikan ekstrak daun kedondong 100 mg/kg bb (0,2 ml/ekor); kelompok C diberikan ekstrak daun kedondong 200 mg/kg bb (0,4 ml/ekor); kelompok D diberikan ekstrak daun kedondong 300 mg/kg bb (0,6 ml/ekor); kelompok E diberikan ekstrak daun kedondong 400 mg/kg bb (0,8 ml/ekor). Pemberian ekstrak daun kedondong dilakukan secara oral. Dilanjutkan dengan nekropsi pada hari ke-15 untuk pengambilan organ hati yang nantinya akan dibuat preparat hitopatologi.Hasil pemeriksaan histopatologi pada hati  tikus putih(Rattus norvegicus) yang diberikan ekstrak etanol daun kedondong, tidak ditemukan adanya perubahan seperti adanya infiltrasi sel radang, degenerasi melemak, degenerasi hidrofik dan nekrosis pada kelompok perlakuan K1, K2, K3, tetapididapatkan hasil peradangan ringan pada kelompok perlakuan K4 dan K5 pada 1 ekor tikus putih (Rattus norvegicus) dari 5 ekor yang diberi perlakuan pada tikus putih (Rattus norvegicus).
Immunological Detection of Avian Influenza Virus in Infected Ducks by Monoclonal Antibodies Against AIV-H5N1 ASTAWA, NYOMAN MANTIK; WINAYA, IDA BAGUS OKA; AGUSTINI, LUH PUTU; HARTANINGSIH, NINING
Microbiology Indonesia Vol 1, No 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

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Abstract

In order to establish a detection method for avian influenza virus (AIV) infection in ducks, monoclonal antibodies (MAbs) against the virus were produced. The virus used for the production of the monoclonal antibodies was AIV-H5N1 of Indonesian origin. Immortal mouse myeloma were fused with the lymphocytes derived from the spleen of mice immunized with the virus. The MAbs were tested for their specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehyde inactivated virus and normal allantoic fluid as a negative control. Twelve MAbs which were specific against AIV were isolated and 8 of them were used for detecting of AIV antigen in duck’s tissues. AIV antigen was detected in paraffin embedded tissues of AIV-infected ducks by immunohistochemistry using MAbs. AIV antigen was not detected in ducks, which were confirmed to be AIV negative. In the infected ducks, high intensity of AIV infection was detected in proventricle gland and small intestine. The AIV antigen with a lesser intensity was also detected in lungs, spleen, and bursa of Fabricius, but hardly detected in muscle, brain, and several other issues. This study shows a clear evidence that MAbs produced in this study are applicable for use in immunological detection of AIV in infected duck tissues.
Histopatologi Ginjal Tikus Putih Akibat Pemberian Ekstrak Pegagan (Centella asiatica) Peroral Suhita, Luh Putu Ratna; Sudira, I Wayan; Winaya, Ida Bagus Oka
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
Publisher : Buletin Veteriner Udayana

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Abstract

Pegagan merupakan tanaman herbal  yang sering dimanfaatkan oleh masyarakat Indonesia sebagai obat alternatif untuk mengobati berbagai macam penyakit. Penelitian tentang toksisitas (studi histopatologi) tanaman pegagan pada ginjal belum pernah dilakukan. Adapun tujuan dari penelitian ini adalah untuk mengetahui adanya perubahan histopaotogi pada ginjal tikus putih setelah pemberian ekstrak pegagan (Centella asiatica) peroral. Tikus putih (Rattus norvegicus) dibagi secara acak menjadi lima kelompok, masing-masing kelompok berjumlah 6 ekor. Kelompok A sebagai control (placebo) yang diberi aquades peroral; kelompok B yang diberikan ekstrak pegagan 100 mg/kg bb (0,2 ml/ekor); kelompok C yang diberikan ekstrak pegagan dosis 200 mg/kg bb (0,4 ml/ekor); kelompok D yang diberikan 300 mg/kg bb (0,6 ml/ekor); dan kelompok E yang diberikan ekstrak pegagan dosis 400 mg/kg bb (0,8 ml/ekor). Nekropsi untuk pengambilan organ ginjal dilakukan pada hari ke-9. Jaringan ginjal selanjutnya diproses untuk pembuatan preparat histopatologi dengan pewarnaan Hemaktosilin Eosin (HE). Hasil pemeriksaan histopatologi pada ginjal tikus putih yang diberikan ekstrak pegagan, tidak ditemukan adanya degenerasi melemak, degenerasi hidrofik, dan nekrosis baik pada control (placebo) maupun pemberian dosis 0,2  ml; 0,4 ml; 0,6 ml; 0,8 ml. Hasil ini menunjukkan pemberian ekstrak pegagan (Centella asiatica) dengan rentang dosis 100 mg/kg bb sampai dengan dosis 400 mg/kg bb selama 9 hari, tidak menyebabkan gangguan histopatologi pada organ ginjal tikus putih (Rattus novegicus).
Pneumonia Verminosa pada Kucing Lokal yang Terinfeksi oleh Aelurotsrongylus sp (VERMINOUS PNEUMONIA IN DOMESTIC CAT INFECTED BY AELUROSTRONGYLUS SP) Winaya, Ida Bagus Oka; Berata, I Ketut; Kardena, I Made; Oka, Ida Bagus Made
Jurnal Veteriner Vol 13, No 4 (2012)
Publisher : Jurnal Veteriner

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Abstract

The aim of the study was to evaluate the pulmo pathological changes of domestic cat infected byAelurostrongylus sp. A total of 15 cats were examined at Faculty of Veterinery Medicine, Udayana Universityduring 2010. Ten out of 15 cats showed sneezing, whereas the remains showed serous rhinitis and sneezing.Macroscopic and microscopic changes were observed mainly on pulmo samples. Hyperemias on caudalislobes and pleura effusion were found in the pulmo. The pulmo tissue was fixed on 10 % neutral bufferformalin and stained with haematoxilin-eosin (HE) for histopathological examination. Aelurostrongylus spwas present in the alveoli lumen of the lung samples. Meanwhile, the alveoli septa of the lung wereobserved thicker and infiltrated with neutrophils, plasma exudates and erythrocytes. Pleural effusion wasmainly consisted of eosinophilic substances. It is concluded that verminous pneumonia in domestic catinfected with Aelurostrongylus sp was an acute infection.
Histopatologi Ginjal Tikus Putih Akibat Pemberian Ekstrak Pegagan(Centella asiatica) Peroral Suhita, Ni Luh Putu Ratna; Sudira, I Wayan; Winaya, Ida Bagus Oka
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : Buletin Veteriner Udayana

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Abstract

Pegagan merupakan tanaman herbal  yang sering dimanfaatkan oleh masyarakat Indonesia sebagai obat alternatif untuk mengobati berbagai macam penyakit. Penelitian tentang toksisitas tanaman pegagan pada ginjal belum pernah dilakukan.Adapun tujuan dari penelitian ini adalah untuk mengetahui adanya perubahan histopatotogi pada ginjal tikus putih setelah pemberian ekstrak pegagan (Centella asiatica) peroral.Tikus putih (Rattus norvegicus)sebanyak 30 ekordibagi secara acak menjadi lima kelompok, kelompok A sebagai control (placebo) yang diberi aquades peroral; kelompok B yang diberikan ekstrak pegagan 100 mg/kg bb (0,2 ml/ekor); kelompok C yang diberikan ekstrak pegagan dosis 200 mg/kg bb (0,4 ml/ekor); kelompok D yang diberikan 300 mg/kg bb (0,6 ml/ekor); dan kelompok E yang diberikan ekstrak pegagan dosis 400 mg/kg bb (0,8 ml/ekor).Nekropsi untuk pengambilan organ ginjal dilakukan pada hari ke-9.Jaringan ginjal selanjutnya diproses untuk pembuatan preparat histopatologi dengan pewarnaan Hemaktosilin Eosin (HE).Hasil pemeriksaan histopatologi pada ginjal tikus putih yang diberikan ekstrak pegagan, tidak ditemukan adanya degenerasi melemak, degenerasi hidrofik, dan nekrosis baik pada kontrol (placebo) maupun pemberian dosis 0,2  ml; 0,4 ml; 0,6 ml; 0,8 ml.Hasil ini menunjukkan bahwapemberian ekstrak pegagan (Centella asiatica) dengan rentang dosis 100 mg/kg bb sampai dengan dosis 400 mg/kg bb selama 9 hari, tidak menyebabkan gangguan histopatologi pada organ ginjal tikus putih (Rattus novegicus).
Kombinasi Lesi Badan Negri, Spongiform, dan Perivascular Cuffing pada Otak Anjing Penderita Rabies (THE COMBINATION OF NEGRI BODIES, SPONGIFORM, AND PERIVASCULAR CUFFING IN RABIES AFFECTED DOG’S BRAIN) Berata, I Ketut; Kardena, I Made; Winaya, Ida Bagus Oka; Supartika, I Ketut Eli
Jurnal Veteriner Vol 15, No 3 (2014)
Publisher : Jurnal Veteriner

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Abstract

A relatively high incidence of spongiform lesions (61%) and perivascular cuffing (89%) in brains ofdogs that suffered from rabies in Bali was an interesting background to do further studied. This study aimwas to identify the association between Negri bodies, spongiform and perivascular cuffing in dog’s brainthat infected with rabies in Bali. The research used 28 of dog’s brain samples infected with rabies and havebeen diagnosed using fluorescence antibody technique (FAT) in the Veterinary Disease InvestigationCenter, of Denpasar. Each of the brain samples contained of cerebrum, cerebellum and hippocampus thatwere taken for histological examination using hematoxylin-eosin staining. The presence of negri bodies,spongiform, and perivascular cuffing were analyzed for their association. The results showed thatcombination lesions of Negri bodies and spongiform found in the cerebellum (14%), cerebrum (4%), andhippocampus (32%), while combination lesions of perivascular cuffing and Negri bodies found in cerebellum(18%), cerebrum (7%), and hippocampus (43%). The highest intensity combination lesion of Negri bodiesand spongiform was found in hippocampus compared to the cerebellum and cerebrum. The highest intensityfor combination of spongiform and perivascular cuffing was found in cerebrum 50%, cerebellum 32%, andhippocampus 36%. It can be concluded that there might be association between the existence of Negribodies and perivascular cuffing lesions with the existed area of rabies infected dog’s brain, but not likely asspongiform.
Pelacakan Secara Imunohistokimiawi Antigen Ekskretori-Sekretori pada Sapi Bali yang Terinfeksi Fasciola gigantica (IMMUNOHISTOCHEMICAL DETECTION OF EXCRET0RY-SECRETORY ANTIGENS IN BALI CATLLE INFECTED BY FASCIOLA GIGANTICA) Winaya, Ida Bagus Oka; Astawa, I Nyoman Mantik; Damriyasa, I Made; Dharmawan, Nyoman Sadra; Berata, I Ketut
Jurnal Veteriner Vol 15, No 3 (2014)
Publisher : Jurnal Veteriner

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Abstract

In order to study the distribution of excretory-secretory (ES) F. gigantica in liver tissue of infected balicattle a research was establisihed using monoclonal antibodies (mAbs) againts ES antigens. Immortalmouse myeloma cells were fused with the lymphocytes derived from the spleen of mice that immunizedwith ES antigen. The mAbs were tested for their specificity by using enzyme linked immunosorbent assay(ELISA). Five specific mAbs againts ES antigens were isolated and two mAbs were used for immunodetectionof ES antigens in liver tissue of bali cattle. Immunohistochemical ES antigens were not detected in paraffinembeded tissue of negative confirmed fasciolosis samples. ES antigens was detected in hepatocytes andcytoplasm of bile duct epithelims in the bali cattle that infected with fasciolosis in moderate intensity.Therfore indicated that mAbs produced in this study are applicable for detecting ES antigens in bali cattleinfected by F. gigantica.