Articles

Prevalensi Kuman ESBL (Extended Spectrum Beta Lactamase) dari Material Darah di RSUP Dr. Kariadi Tahun 2004-2005

MEDIA MEDIKA INDONESIANA 2009:MMI VOLUME 43 ISSUE 5 YEAR 2009
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

Prevalence of Extended-Spectrum  -Lactamases (ESBL)-bacteria of Blood Isolates in Dr. Kariadi Hospital Semarang 2004–2005Background: Prevalence of ESBL bacteria varies among hospitals, which its resistance could be spread to other bacteria; causes infections with a high morbidity and mortality. There were no data of ESBL prevalence and its distribution within Dr. Kariadi hospital wards. Objective of the study were to determine the prevalence, distribution and antibiotic sensitivity pattern of ESBL bacteria in Dr. Kariadi hospital.Methods: Retrospective study was used on laboratory records of in-patients at Clinical Microbiology Laboratory during 2004–2005. Blood culture was inoculated into BACTEC bottle medium, incubated at 370C, isolated, identified and examined for antibiotic susceptibility by Kirby-Bauer method. Inclusion criteria were gram negative bacteria subjected to ESBL screening by cefotaxime, ceftazidime or ceftriaxone disc. Samples without clinical informations were excluded.Results: Four thousand three hundred and fifty blood samples were examined during 2 years periode with culture positive rate was 34.76% consist of gram negative bacteria 59.6% in which ESBL bacteria was 50.6%. ESBL bacteria significantly high recovered from intensive wards. Predominance bacteria were Ps. aeruginosa (50.9%), E. aerogenes (37.5%) and E. coli (8.7%). Sensitivity patterns to meropenem >82.2%, quinolone >65.6% except Ps. aeruginosa 52.5%, fosfomisin >74% except Ps. aeruginosa 15.5%,amikacin >82% except Ps. aeruginosa 20.6%.Conclusions: Bacterial culture positive rate was 34.76% with predominance bacteria was gram negative bacteria (59.6%), in which ESBL detected in 50.6%. The most predominance bacteria were Ps. aeruginosa, E. aerogenes and E. coli. Antibiotic sensitivity patterns mostly sensitive to meropenem, aminoglycocide and quinolone.Keywords: ESBL, intensive wards, meropenem ABSTRAKLatar belakang: Prevalensi kuman ESBL berbeda diberbagai rumah sakit, mempunyai gen penyandi di plasmid yang mudah dipindahkan ke kuman lain dengan morbiditas dan mortalitas tinggi. Di RSUP Dr. Kariadi belum ada data komprehensif tentang prevalensi dan distribusi kuman ESBL. Maksud penelitian untuk memberikan gambaran tentang prevalensi, distribusi dan pola kepekaan kuman ESBL.Metode: Penelitian bersifat retrospektif menggunakan catatan pemeriksaan darah pasien rawat inap yang dikultur menggunakan BACTEC di Laboratorium Mikrobiologi Klinik tahun 2004-2005. Tes kepekaan menggunakan metode Kirby-Bauer. Kriteria inklusi ialah kuman gram negatif yang resisten terhadap salah satu atau lebih dari disk antibiotika cefotaxim, ceftazidime dan cefipim, sedang kriteria eksklusi adalah keterangan klinik yang tidak lengkap.Hasil: Didapat 4.350 sampel, yang tumbuh kuman sebanyak 1.512 (34,76%) terdiri dari kuman gram positif 611 (40,4%) dan kuman gram negatif 901 (59,6%), diantaranya kuman ESBL sebanyak 456 (50,6%). Kuman ESBL di ruang perawatan intensif lebih banyak dibandingkan ruang non-intensif (p=0,00 chi square test). Prevalensinya adalah Ps. aeruginosa (50,9%), E. aerogenes (37,5%), E. coli (8,7%), K. pneumoniae (1,5%), A. baumini (1,1%) dan E. clocae (0,3%). Sensitifitas kuman terhadap antibiotika: meropenem >82,2%, kuinolon >65,6% kecuali Ps. aeruginosa 52,5%, fosfomisin >74% kecuali Ps. aeruginosa 15,5%, amikasin >82% kecuali Ps. aeruginosa 20,6%.Simpulan: Hasil kultur positif 34,76%, kuman ESBL didapatkan 50,9% dengan predominan Ps. aeruginosa, E. aerogenes dan E. coli. Kuman ESBL di ruang perawatan intensif lebih banyak, dengan sensitifitas antibiotika yang masih baik ialah meropenem, aminoglikosida dan kuinolon.

Ekstrak Keladi Tikus (Typhonium flagelliforme) Fraksi Diklorometanolik dan Ekspresi Caspase-3 dan p21 Cell-Line Kanker Payudara MCF-7

MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 2 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTThe dichloromethanolic fraction of keladi tikus (Typhonium flagelliforme) extract and the expression of p21 and caspase-3 of MCF-7 breast cancer cell-lineBackground: Breast cancer has a high degree of heterogeneity and problems in therapy, such as insensitive to antiproliferation signal and able to escape from apoptosis program. Typhonium flagelliforme (Lodd), Blume (Araceae) more commonly known as keladi tikus in Indonesia, often used as alternative in treating cancer, including breast cancer. The objective of study is proving the dichloromethanolic fraction of Typhonium flagelliforme tuber extracts influence on the expression of caspase-3 and p21 of MCF-7 cell-line that have deletion in CASP-3 gene.Methods: This study adapts laboratory experimental in-vitro in cells-line MCF-7, with “post test control group only design” and divided into two groups, were incubated in 5h, 10h and 20h. The control group received no other treatment. The treatment group received the dichloromethanolic fraction of Typhonium flagelliforme tuber extracts in IC50. Starting with cytotoxicity assay using MTT to determine the IC50, followed by Immunocytochemical analysis to observe the expression of p21 and caspase-3.Results: Paired sample T test shows a significant differences in treatment groups compared with the controls (p<0.05), namely an increase in the expression of nuclear p21 and caspase-3 on 20h incubation.Conclusion: There were increased in the expression of caspase-3 and nuclear p21, as well as depleted the cytoplasmic of p21 on MCF-7 cell-line that have deletion in CASP-3 in treatment by tuber extract of Typhonium flagelliforme dichloromethanolic fraction.Keywords: p21, caspase-3, MCF-7 cell-line, Typhonium flagelliformeABSTRAKLatar belakang: Kanker payudara mempunyai tingkat heterogenitas tinggi dan problem dalam terapi, diantaranya tidak sensitif terhadap signal antiproliferasi dan mampu menghindar dari program apoptosis. Typhonium flagelliforme (Lodd). Blume (Araceae) yang lebih dikenal sebagai keladi tikus di Indonesia, sering digunakan sebagai alternatif dalam mengobati penyakit kanker,termasuk kanker payudara. Tujuan penelitian ini untuk membuktikan pengaruh Typhonium flagelliforme terhadap ekspresi caspase- 3 dan p21 pada cell-line MCF-7 yang mengalami delesi gen CASP-3.Metode: Penelitian eksperimental laboratorik secara invitro pada cell-line MCF-7, dengan rancangan penelitian post test control group only design, yang dibagi dalam 2 kelompok dan diinkubasi dalam 5 jam, 10 jam dan 20 jam. Kelompok kontrol tidak mendapatkan perlakuan, sedangkan kelompok perlakuan mendapatkan ekstrak umbi Typhonium flagelliforme fraksi diklorometanolik kadar 62,08 μg/mL(IC50). Dimulai dengan uji sitotoksisitas menggunakan MTT untuk menentukan IC50, dilanjutkananalisis imunositokimia untuk melihat ekspresi p21 dan caspase-3.Hasil: Paired sample T test menunjukkan terdapat perbedaan bermakna pada kelompok perlakuan dibandingkan kontrol (p<0,05), dimana terjadi peningkatan ekspresi caspase-3 dan p21 nukleus serta deplesi p21 sitoplasmik pada inkubasi 20 jam.Simpulan: Ekstrak umbi Typhonium flagelliforme fraksi diklorometanolik mampu meningkatkan ekspresi caspase-3 dan p21 nukleus serta deplesi p21 sitoplasmik cell-line MCF-7 yang mengalami delesi gen CASP-3.

Penentuan LD50 Penyakit Infeksi Toxoplasma gondii pada Mencit balb/c

MEDIA MEDIKA INDONESIANA 2009:MMI VOLUME 43 ISSUE 4 YEAR 2009
Publisher : MEDIA MEDIKA INDONESIANA

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LD50 determination in toxoplasma gondii infection in balb/c miceBackground: LD50 is a marker of virulence which is reflects degree of microorganism pathogenicity. LD50 of every pathogens to be used should be determined in order to choose an appropiate infective dose of agent under the study. Toxoplasma gondii RH strain has been maintained in the laboratory by serial passage in mice for several years, in which the biologic and laboratory conditions might has an effect on its virulence. The objective of this study was to determine LD50 of Toxoplasma gondii RH strain used for research in Biotechnology Laboratory Gajah Mada University.Methods: The design of the study was true experiment consisting three goups of 10 female balb/c mice aged 8–10 weeks that were infected with 101, 103 and 106 of Toxoplasma gondii RH strain/mice respectively, and being followed up to day 18. Homogeneity of mice before infection were analyzed by Levence statistic, while the effects of infection was analyzed by Anova. The number of dead mice in each group were recorded and LD50 was calculated base on proportional distance.Results: There was no weight difference between groups of mice before infection. The weight increased up to day 6 in group I and II, while group III after day 3 there was weight decrease. LD50 of Toxoplasma gondii was 1.39x103 which belonged to genotype II of pathogenicity classification.Conclusions: LD50 of Toxoplasma gondii was 1.39x103 which is lower than the original RH strain, and belonged to group II of genotype, which is often reported as the major cause of human and animal infections.Keywords: LD50, Toxoplasma gondii, balb/c mice, infection. ABSTRAKLatar Belakang: Toxoplasma gondii di laboratorium dipelihara dengan cara pasase dari satu mencit ke mencit berikutnya, yang mungkin berpengaruh terhadap virulensinya. LD50 adalah ukuran virulensi suatu mikroorganisme penyebab infeksi, yang harus diketahui apabila akan melakukan penelitian tentang fenomena infeksi pada binatang coba. Tujuan penelitian ini untuk menentukan besar LD50 Toxoplasma gondii strain RH yang disimpan dan digunakan untuk penelitian di Laboratorium Bioteknologi UGM yang belum pernah ditentukan nilai LD50nya.Metode: Disain penelitian adalah eksperimental murni. Tiga kelompok mencit balb/c usia 6–8 minggu masing-masing kelompok terdiri dari 10 mencit, diinfeksi Toxoplasma gondii strain RH secara berturut-turut tiap kelompok diinfeksi dengan 101, 103 dan 106 toksoplasma/mencit, yang diamati sampai hari ke-18. Sebelum perlakuan ketiga kelompok dilakukan tes homogenitas berat badan mencit dengan statistik Levene. Efek infeksi toksoplasma dianalisis dengan Anova, sedangkan LD50 dihitung berdasarkan jarak proporsi.Hasil: Sebelum diinfeksi Toxoplasma gondii, tidak terdapat perbedaan berat badan mencit pada 3 kelompok. Setelah diinfeksi toksoplasma sampai hari ke-6, berat badan mencit pada kelompok I dan II naik, sedangkan kelompok III pada hari ke-1 sampai hari ke-3 beratnya naik, kemudian setelah hari ke-3 beratnya turun. LD50 Toxoplasma gondii strain RH di PAU Bioteknologi UGM didapatkan sebesar 1,39x103 yang sesuai dengan genotipe II.Simpulan: LD50 Toxoplasma gondii sebesar 1,39x103 lebih rendah dari strain RH yang sebenarnya dan termasuk genotipe II yaitu yang paling sering dilaporkan sebagai penyebab infeksi pada manusia dan binatang.

The Effectiveness of Typhonium flagelliforme Tuber Extract of Diclorometanolic fraction on The Inhibition of Proliferation of MCF-7 Human Breast Cancer Cell-Line

Journal of the Indonesian Medical Association Vol. 62 No. 1 January 2012
Publisher : Journal of the Indonesian Medical Association

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Introduction: Breast cancer has been shown to have the ability to survive by proliferation.Typhonium flagelliforme (Lodd), known as keladi tikus has ability of anti-proliferation on themurine P388 leukemia cell and NCI-H23 non-small cell lung carcinoma cell line, depend on thepolarity of solvent and structure of cancer cell. The aim of this study was to compare the effectivenessof tuber and leave extract of Typhonium flagelliforme to inhibit the cell proliferation of MCF-7 breast cancer cell line.Methods: in-vitro experimental study on the MCF-7 cell “Post test control group only design” the cell lines were divided into 49 groups that include the control group, 3 groups treated with DMC, ethanolic, hexanolic extract of Typhonium flagelliforme and 3 groups treated withDCM, ethanolic , and hexanolic extract of the leave of Typhonium flagelliforme at concentrationof 1000, 500, 250, 125, 62,5, 31,25, 15,62, 7,81μg/m. Cell proliferation was estimated byMTT assay, log probit was used for the statistical analysis.Results: DMC extract of the tuber of Typhonium flagelliforme inhibit the cell proliferation at IC5o= 63.08 μg/mL (nilai p<0.001), and the DMC extract of the leave of Typhonium flagelliformeinhibit cell proliferation at IC5o = 68.65 μg/mL.Conclusion: The tuber extract of Typhonium flagelliforme is a better potent inhibitor of proliferationin the MCF-7 breast cancer cell line compared to the leave extract. J Indon Med Assoc.2012;62:10-5.Keywords: antiproliferation, Typhonium flagelliforme, MTT Method, IC50

Karakteristik Dinamik Kolom Baja yang Diisi Pasir, Kerikil atau Serbuk Gergaji Besi

Jurnal Teknik Mesin Vol 10, No 2 (2008): OCTOBER 2008
Publisher : Institute of Research and Community Outreach - Petra Christian University

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Steel column with circular cross section is frequently used in both civil and machine construction. Many of girders of ballyhoo, traffic sign pillars etc. are made from the type of this column, because of good availability and low price. The machine tool construction e.g. a drilling machine actually should be built with box type column not a circular one. However, in order to be able to rotate the machine arm and or the table, the machine column must be made of a circular type. Whereas a circular type column has both static and dynamic either bending or torsional stiffness lower than the box type for the same weight. The research is aimed to increase the dynamic stiffness of circular type column by using granular material as a vibration damper. The granular material like sand, gravel and iron sawdust is investigated. The result shows that the more volume of the granular material to be filled in column, the higher both the damping ratio and the dynamic stiffness. Consequently the best result is achieved by fully filled condition. The damping ratio rises up from 1 % for the an empty column to 10.6 % or up to 10.6 times higher for fully filled with iron sawdust and to 8.6 % or up to 8.6 times higher for fully filled with sand. The dynamic stiffness increase from 4.427E+5 N/m for an empty column to 5.285E+6 N/m or up to 11.94 times higher for fully filled with iron sawdust and to 2.192E+6 N/m or up to 4.95 times higher for fully filled with sand. This is of course a fantastic successfulness for a simple treatment. Abstract in Bahasa Indonesia: Kolom yang berpenampang lingkaran banyak dipakai dalam konstruksi baik mesin maupun sipil. Banyak penyangga papan reklame, tiang rambu lalu-lintas dan sebagainya terbuat dari kolom jenis ini. Kolom mesin perkakas seperti kolom mesin drill sebenarnya dan seharusnya dibuat dari kolom yang berpenampang kotak (box) bukan berpenampang lingkaran seperti pipa, tetapi agar lengan (arm) dapat diputar atau meja mesin tersebut dapat diputar dengan tujuan meningkatkan fleksibilitas seting benda kerja, maka struktur mesin tersebut dibuat dari kolom yang berpenampang lingkaran. Padahal kolom berpenampang lingkaran mempunyai karakteristik bahwa kekakuan statik dan dinamik baik bending maupun torsi jauh lebih rendah dibandingkan dengan kolom yang berpenampang kotak untuk berat yang sama. Dengan harapan terjadi peningkatan kekakuan yang besar, maka dilakukanlah penelitian dengan menggunakan pengisian material butiran sebagai peredam getaran, yaitu pasir dengan ukuran butiran kecil (halus), sedang dan besar (kerikil) serta serbuk besi. Hasil percobaan memperlihatkan bahwa semakin besar volume pengisian material butiran, maka semakin besar pula peningkatan baik redaman maupun kekakuan dinamiknya. Peningkatan rasio redaman dari 1% untuk kolom kosong hingga 10,6% untuk isi serbuk besi penuh dan 8,6% untuk isi pasir halus penuh. Jadi terjadi peningkatan rasio redaman 10,6 kali dan 8,6 kali masing-masing. Kekakuan dinamik terjadi kenaikan dari 4.427E+5 N/m menjadi 5.285E+6 N/m untuk serbuk besi penuh dan 2.192E+6 N/m untuk pasir halus penuh, sehingga peningkatan kekakuan dinamik yang dicapai adalah 11,94 kali dan 4,95 kali masing-masing. Ini merupakan hasil yang sangat fantastis untuk perlakuan yang sederhana. Kata kunci: Kolom, kerikil, serbuk besi, kekakuan dinamik, perbandingan redaman.

ANALISIS SPASIOTEMPORAL KASUS DEMAM BERDARAH DENGUE DI KECAMATAN NGALIYAN BULAN JANUARI-MEI 2012

MEDIA MEDIKA MUDA 2012:MMM VOLUME 1 NUMBER 1 YEAR 2012
Publisher : MEDIA MEDIKA MUDA

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Latar belakang: Penyakit DBD masih merupakan masalah dalam kesehatan masyarakat dan menimbulkan dampak sosial maupun ekonomi. Hal ini karena DBD adalah penyakit dengan angka kesakitan dan kematian yang masih tinggi, terutama di Kecamatan Ngaliyan, Semarang. Oleh karena itu diperlukan surveilens pemetaan distribusi serta analisis spasial dan temporal kasus DBD untuk mengarahkan intervensi terbaik demi pencegahan penyakit DBD.Tujuan: Mendapatkan gambaran distribusi spasial dan temporal kasus DBD di kecamatan Ngaliyan.Metode: Penelitian dengan disain eksploratif menggunakan data primer dan sekunder. Data primer didapatkan melalui GPS dengan output letak lintang dan bujur tempat tinggal penderita. Data sekunder merupakan keadatan penduduk, curah hujan, suhu dan kelembaban udara serta peta kota Semarang. Data diproses dengan menggunakan program Microsoft Excel 2007 dan ArcGis 9.2Hasil: Penelitian ini mendapatkan 39 penderita DBD yang tinggal di Kecamatan Ngaliyan pada bulan Januari-Mei 2012 terdiri dari 21 laki-laki dan 18 perempuan. Kelurahan Kalipancur merupakan kelurahan dengan insidensi DBD tertinggi (38,46%). Bulan Maret merupakan bulan dengan angka kasus DBD tertinggi (33,33%). Umur penderita berkisar 5 bulan-36 tahun dengan angka tertinggi pada kelompok umur 5-14 tahun (51,28%).Simpulan: Didapatkan adanya hubungan antara angka kejadian DBD dengan kepadatan penduduk serta didapatkan adanya pengelompokan kasus DBD di wilayah dengan kepadatan penduduk tinggi. Akan tetapi tidak didapatkan hubungan antara angka kejadian DBD dengan curah hujan, suhu rata-rata serta kelembaban udara. Pencegahan kasus DBD sebaiknya difokuskan pada aspek kebersihan lingkungan.Kata Kunci: DBD, analisis spasiotemporal, SIG

EMS-45 Tool Steels Hardenability Experiment using Jominy ASTM A255 Test Method

IPTEK The Journal for Technology and Science Vol 24, No 1 (2013)
Publisher : Institut Teknologi Sepuluh Nopember

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Hardenability of steels is an important way to determine heat treatment and material properties that produce component products. Jominy test is one of the method to know hardenability of steels. The Jominy ASTM A255 in used as a method for carriying out and this reseach. Parameter such as austenite temperature, holding time, cooling rate and then the results is dedicated by the prediction result, with Non Linear Numerical Equation Method. Based on test, it’s known, increasing austenite temperature, longer holding time and high cooling rate, will increase hardenability of steels. The different between the results and the prediction result done by Sonh Yue-Peng[15], Matja equation[14] and Zehtab equation[10], about 5 % -10 %. The data obtained from this experiment can be used to determine the appropriated heat treatment in order to get the desired mechanical properties, as well as to avoid distortion.

Engineering Design of A Gang Drilling Machine Equipped with Jig and Fixtures to Make A Prototype Machine in Birdcage Production

IPTEK The Journal for Technology and Science Vol 22, No 4 (2011)
Publisher : Institut Teknologi Sepuluh Nopember

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This paper is dealing with the engineering design of a gang drilling machine with jig & fixtures to make a prototype machine. This effort has been done in order to solve the problem which aroused in small business enterprises producing birdcages. The problem was how to minimize the production time in making a lot of holes that have same distance and straightness. Hopefully, the prototype machine can help the small business enterprises to increase their production rate.The design engineering process has been carried out by variant approximation on dowel pin modular fixtures in order to simplify fixtures design. CAD CAM software has also been used as fixtures synthesized method including geometric analysis and three dimensional fixtures assembling. The resulting prototype machine can be well operated and based on the running test, it can be concluded that the greater the motor rotation the greater the power needed. As for teak wood, at 250 rpm motor rotation the power needed is 26.5 watt, and at 400 rpm the motor needs power of 43.6 watt while at 600 rpm the motor needs power of 600 watt. The power consumption is also depends on the type of material, the better the mechanical properties of the materials, the higher the power consumption. For cast iron, the 400 rpm motor rotation needs power as high as 569.7 watt. This prototype of gang drilling machine needs power of 350 watt to make five holes on teak wood while ordinary drilling machine needs total power of 1350 watt.

Keragaman Komunitas Fitonematoda pada Sayuran Lahan Monokultur dan Polikultur di Sumatera Barat

Jurnal Fitopatologi Indonesia Vol 9, No 3 (2013)
Publisher : Jurnal Fitopatologi Indonesia

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Plant parasitic nematodes can cause crop losses in celery. Parasitic nematodes that infect celery are Helicotylenchus spp., Trichodorus, Longidorus, Xiphinema and Meloidogyne spp Cropping pattern becomes part of the factors that influence the presence of plant parasitic nematodes in celery. Field obervation was conducted in celery growing areas to study the diversity and abundance of parasitic nematodes from monoculture and polyculture cropping system. Seven genus of nematodes, i.e. Meloidogyne, Pratylenchus, Tylenchorhinchus, Tylenchulus, Xiphinema, and two nonparasitic nematode were identified from polyculture cropping system, whereas 4 genus were found from monoculture cropping system i.e. Meloidogyne, Pratylenchus, Tylenchulus and Trichodorus. Genus with high abundance in polyculture cropping pattern was Xiphinema and Meloidogyne however Trichodorus was not found. Genus with a high abundance in monoculture was Meloidogyen and Xiphinema was not found. The genus most frequently found was Meloidogyne in polyculture and Xiphinema in monoculture.

OTOMASI PENGUKURAN DAN KONTROL PADA ALAT UJI MEKANIK BAHAN BERBASIS INSTRUMENTASI VIRTUAL

Physics Student Journal Vol 1, No 1 (2013)
Publisher : Department of Physics - Faculty of Science

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Pengoperasian alat uji mekanik bahan yang dimiliki Jurusan Fisika UB masih dilakukan secara manual. Seringkali timbul perbedaan kecepatan yang berpengaruh terhadap tingkat akurasi dan presisi dalam pengukuran terhadap spesimen. Alat ini juga dilengkapi digital force gauge yang mampu mengukur gaya maksimal sebesar 50 N (yang digunakan maksimal sebesar 45 N). Tujuan dari penelitian adalah memodifikasi sistem penggerak pada alat uji mekanik bahan dengan implementasi kecepatan terkontrol yang mampu menarik beban hingga 45 N, membaca data keluaran dari digital force gauge dengan NI-VISA USB, dan mengetahui respon digital force gauge terhadap alat uji mekanik bahan yang telah dimodifikasi. Modifikasi dilakukan dengan menambahkan motor stepper yang dihubungkan ke hand wheel dengan roda gigi. Motor dikendalikan dengan program LabVIEW yang mengirimkan pulsa-pulsa digital ke driver motor stepper. Selain itu, program LabVIEW melalui interface NI-VISA USB digunakan untuk membaca data keluaran digital force gauge dan juga digunakan sebagai umpan balik. Hasil pengujian menunjukkan bahwa modifikasi alat uji mekanik bahan berhasil menarik beban hingga 45 N. Di sisi lain program LabVIEW tidak berhasil membaca keluaran digital force gauge.Kata kunci : Alat uji mekanik bahan, digital force gauge, LabVIEW.