Adi Winarto
Departemen Anatomi Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor

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Aktivitas NADH- tetrazolium reductase sel sel trofoblas pada blastosis yang mengalami hatching dan gagal hatching Helmita, Roza; Djuwita, Ita; Purwantara, Bambang; Winarto, Adi
Jurnal Anatomi Indonesia Vol 1, No 2 (2007)
Publisher : Jurnal Anatomi Indonesia

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Abstract

Implantation is themost critical stage in the establishment of pregnancy. Inmammals, it has been estimated thatbetween 25%and 60%of conceptuses are lost before or at the time of implantation. The objectives of this studywere to investigate the activity of themitochondrial NADH-tetrazoliumreductase, the outgrowth and differentiationof trophoblast cells in in vitro culture of hatched and non hatched blastocyst. Blastocysts were collectedfrom mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatchingwithin 24 hours, 48 hours and non hatching. Embryos were cultured in DMEMmediumin 5%CO2 incubator at37°C for 10 days.The trophoblastsmonolayer were processed forGimsa staining and histochemistry analysisof NADH-tetrazolium reductase activity. The outgrowth of trophoblast cells were measured using eyespiecemicrometer. The results showed that the activity of NADH-tetrazolium reductase of 24 h and 48 h hatchedblastocysts showed higher intensity than the non hatched blastocysts (P<0,05). The trophoblast outgrowthdiameter of 24 h hatched blastocystwas significantly higher than the non hatched blastocyst, but not signifcantlydifferent with the 48 h hatched.Morphology examination using light microscope showed that the trophoblastmonolayer of 24 h hatched blastocyst differentiated into cytotrophoblast, syncytioptrophoblast andspongiotrophoblast after 5 days of in vitro cultured. In conclusion, in in vitro sudy the failure of blastocystsimplantationwas due to the impairment ofNADHdehydrogenase activity in complex Imitochondria and the failureof outgrowth and differentiation of the trofoblast cells.
DINAMIKA FOLIKEL OVARIUM DOMBA PASCATRANSPLANTASI INTRAUTERIN PADA KELINCI PSEUDOPREGNANST Sumarmin, Ramadhan; Winarto, Adi; Yusuf, Tutty Laswardi; Boediono, Arief
Majalah Ilmiah Peternakan Vol 10, No 3 (2007)
Publisher : Majalah Ilmiah Peternakan

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ABSTRAK Tujuan penelitian ini adalah untuk mengevaluasi dinamika folikel pada ovarium domba pascatransplantasi secara intrauterin pada kelinci pseudopregnansi. Transplantasi dilakukan pada kelinci pseudopregnant hari ke 1 atau ke 7. Ovarium kembali diambil pada hari ke 5, 7, atau 9 setelah transplantasi. Untuk menentukan dinamika folikel pada ovarium domba pascatransplantasi dan menghitung jumlah folikel pada berbagai tahap perkembangan, ovarium domba pascatransplantasi dijadikan preparat histologis dengan metode parafin dan pewarnaan HE. Hasilnya masih ditemukan semua tahapan perkembangan folikel (folikel primordial, primer, preantral, dan antral) pada semua kelompok perlakuan. Jumlah folikel pada 5, 7 atau 9 hari pascatransplantasi menurun nyata (p<0,05) kecuali jumlah folikel primordial pada kelompok 5 hari pascatransplantasi (634,7±56,88) tidak berbeda nyata dengan kontrol (683,7±61,55). Dapat disimpulkan bahwa dinamika folikel ovarium domba pascatransplantasi pada kelinci pseudopregnansi masih dapat ditemukan pada semua kelompok perlakuan. THE FOLLICLE DYNAMICS OF EWE OVARIAN POST-INTRAUTERINE TRANSPLANTATION TO PSEUDOPREGNANCY RABBIT ABSTRACT The objective of this study was to evaluate the follicle dynamics of ewe ovarium post-intrauterine transplantation to pseudopregnanty rabbit. The experiment was concerned with the 1st or 7th days of pseudopregnancy to receive the ewe ovarium. After 5, 7, and 9 days transplantation the ewe ovarium were recollected. In order to determine the follicle dynamics of ewe ovari post-intrauterin transplantation and to count the number of each stage, the ewe ovari was prepared using the paraffin methods and staining with HE. The results showed all stages of the follicle dynamics (Primordial, Primary, Preantral and Antral follicle stages) were still found in all groups of treatment. The number of follicles decreased significantly (p<0.05) except the number of Primordial follicles of the 5 days post transplantation (634.7±56.88) was not significantly different (p<0.05) compared to the control group (683.7±61.55). It could be concluded that the follicle dynamics of ewe ovarium post-intrauterine transplantation in pseudopregnancy rabbit still found in all groups of the treatment.
VIABILITAS OOSIT DOMBA PASCATRANSPLANTASI OVARIUM DOMBA DALAM UTERUS KELINCI PSEUDOPREGNANT Sumarmin, Ramadhan; Winarto, Adi; Yusuf, Tutty Laswardi; Boediono, Arief
Majalah Ilmiah Peternakan Vol 11, No 1 (2008)
Publisher : Majalah Ilmiah Peternakan

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ABSTRAK Telah dilakukan penelitian untuk mengetahui viabilitas oosit domba yang dikoleksi dari ovarium domba pascatransplantasi intrauterus pada kelinci pseudopregnant. Ovarium domba ditransplantasikan dalam uterus kelinci pseudopregnant pada hari pertama bunting semu dan kemudian diambil kembali setelah lima (P5) atau tujuh (P7) hari transplantasi. Sebagai kontrol digunakan ovarium segar. Oosit dikoleksi dari ovarium pascatransplantasi dengan metode slicing (pencacahan) di dalam media phosphate buffer saline (PBS) yang disuplementasi dengan 5% fetal bovine serum (FBS) dan 100 IU penicillin-streptomisin/ml. Oosit hasil koleksi selanjutnya dimaturasi dalam Tissue Culture Medium (TCM)-199 yang disuplementasi dengan 10% FBS, 10 IU follicle stimulating hormone (FSH)/ml dan 100 IU penicillin-streptomisin/ml. Oosit selanjutnya diinkubasi dalam inkubator CO2, dengan kandungan CO2 5% dan suhu 38ºC, selama 24 jam. Setelah dimaturasi, oosit diwarnai dengan aceto-orcein 2% untuk menentukan status inti oosit. Hasilnya memperlihatkan bahwa oosit yang mampu mencapai tahapan perkembangan dengan status inti M-II setelah maturasi pada P5 (35,05%) dan P7 (35,24%) nyata lebih sedikit (p<0,05) dibandingkan dengan kontrol (56,65%). Dapat disimpulkan bahwa viabilitas oosit domba pascatransplantasi di dalam uterus kelinci pseudopregnant masih ditemukan meskipun persentasenya lebih rendah. EWE OOCYTE VIABILITY FROM EWE OVARIAN AFTER INTRAUTERINE TRANSPLANTATION TO PSEUDOPREGNANT RABBIT ABSTRACT The aim of the present study was to investigate the ewe oocyte viability from ewe ovary after intrauterine transplantation to pseudopregnant rabbit. The ewe ovary was transplanted intrauterine on day 1 to pseudopregnant rabbit and oocytes recollected on five (P5) or seven (P7) days posttransplantation. The fresh ovary was used as the control. The oocytes were collected from the ovaries by slicing method in Phosphate Buffer Saline (PBS) supplemented with 5% of Fetal Bovine Serum (FBS), and 100 IU/ml of penicillin-streptomycin. Oocytes were matured in Tissue Culture Medium (TCM)-199 supplemented with 10% FBS, 10 IU/ml of Follicle Stimulating Hormone (FSH), and 100 IU/ml of penicillin-streptomycin. Oocytes were incubated in CO2 incubator with 5% CO2, 38°C for 24 h. After maturation, the oocytes were stained with 2% aceto-orcein to determine the nuclear oocytes status. The result showed that the oocytes could reach the M-II phase from P5 (35.05%) and P7 (35.24%) decreased significantly (p<0.05) compared to the control (56.65%). However it can be concluded that the oocytes viability still preserved intrauterine in pseudopregnant rabbit.
Studi Mikroanatomi Pankreas Kodok Lembu Menggunakan Metode Pewarnaan Baku dan Immunohistokimia (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS) Adnyane, I Ketut Mudite; ., Supratikno; Winarto, Adi; Agungpriyono, Srihadi
Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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Abstract

Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.
SEL PENGHASIL LISOZIM TERDETEKSI PADA KELENJAR LUDAH SAPI DENGAN TEKNIK IMUNOHISTOKIMIA LYZOZYME PRODUCING CELLS ARE DETECTED IN THE BOVINE SALIVARY GLAND BY IMMUNOHISTOCHEMICAL TECHNIQUE Mudite Adnyane, I Ketut; Novelina, Savitri; Wresdiyati, Tutik; Winarto, Adi; Agungpriyono, Srihadi
Jurnal Veteriner Vol 8, No 1 (2007)
Publisher : Jurnal Veteriner

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Lisozim bersifat bakteriolotik dengan cara merusak ikatan glikosida pada dinding bakteri sehingga pelacakan sel penghasil lisozim pada kelenjar ludah sapi perlu dilakukan. Dalam penelitian ini, sel penghasil lisozim diperiksa menggunakan teknik pewarnaan histologi dan imunohistokimia. Hasil penelitian menunjukkan bahwa kelenjar parotis sapi bersifat serous murni. Kelenjar submandibularis bagian asinarnya terdiri atas sel-sel serous dan sel-sel mukous. Sel-sel asinar mukous terlihat lebih dominan jika dibandingkan dengan sel-sel asinar serous. Sel-sel asinar kelenjar parotis sapi bereaksi positif dengan intensitas sedang (++) terhadap pewarnaan imunohistokimia. Sel-sel epitel duktus kelenjar bereaksi sedang (++) sampai dengan kuat (+++). Pola sebaran lisozim yang terdeteksi pada penelitian ini menunjukkan kemiripan dengan hewan mamalia pada umumnya. Sel-sel asinar serous pada kelenjar submandibularis memberikan reaksi positif dengan intensitas rendah (+) sampai dengan sedang (++) dan sel-sel epitel duktus kelenjar dengan intensitas kuat (+++). Lisozim ditemukan pada bagian sel-sel asinar serous dan sel-sel epitel duktus kelenjar parotis dan submandibularis.
THE DEVELOPMENT OF FOLLICLES AND OOCYTES VIABILITY FROM EWE OVARIUM POST-INTRAUTERINE TRANSPLANTATION TO PSEUDOPREGNANT RABBIT Sumarmin, Ramadhan; Winarto, Adi; Laswardi Yusuf, Tutty; Boediono, Arief
Jurnal Veteriner Vol 9, No 3 (2008)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to evaluate the development of ewe follicles and oocytes viabilityfollowing an intrauterine transplantation of the ovaries into pseudopregnant rabbit. The experiment wasconducted in day 1 of pseudopregnant rabbit. Following transplantation for 5, 7, and 9 days, the ovarieswere recollected. The development of follicles determined by counting the number of follicles in paraffinembedded ovaries after staining with haematoxylin-eosin (HE). The viability of oocytes was determined byslicing the ovaries. Oocytes were incubated in CO2 incubator with 5% CO2, 38°C for 24 hours. After maturation,the oocytes were stained with 2% aceto-orcein to determine the nuclear oocytes status. The result showedthat follicles were detected in all stages of their development (primordial, primary, preantral, and antralfollicle stages), but their number decreased significantly (P<0.05) 5, 7 or 9 days after transplantation,except for those at primordial stage which at day 5 post-transplantation (634.7±56.88) were not significantlydifferent to the control (683.7±61.55). After maturation, the oocytes that were able to reach the M-II phaseat day 5 and day 7 post-transplantation were 35.05% and 35.24% respectively. They were significantly(P<0.05) lower than the control (56.65%). In conclusion, the development of follicles and oocytes viability inthe ewe ovaries in pseudopregnant rabbits was still preserved during intrauterine transplantation.
Kajian In vitro Aktivitas Sel-Sel Trofoblas Blastosis Mencit Aging dan Pengaruhnya terhadap Kegagalan Implantasi Djuwita, Ita; Helmita, Roza; Winarto, Adi; -, Wahyudin
Jurnal Veteriner Vol 10, No 1 (2009)
Publisher : Jurnal Veteriner

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Abstract

The objectives of this in vitro study were to investigate the hatching rate, the outgrowth diameter andthe activity of mitochondria Nicotiamide Adenin Dinucleotide Dyhidrogenage (NADH)-CoQ reductase ofblastocysts trophoblast cells from aging mice. Blastocysts of aging (age >12 months) and young productive(age 2 months) mice were collected from the cornua utery at day-4 of pregnancy and were cultured inmDMEM medium supplemented with 10% New Born Calf Serum (NBCS), 10% ITS, and 50 ?g/mlgentamicine, in 5% CO2 incubator at 37°C for 10 days. The blastocysts hatching rate and the trophoblastsmonolayer were examined for their diameter outgrowth and the NADH-CoQ reductase activity. The resultsshowed that the hatching rate, the trophoblast outgrowth diameter and the activity of NADH-CoQ reductaseof blastocysts collected from productive mice were significantly higher than those collected from the agingmice (P<0,05). It can be concluded that the impairment of blastocysts implantation especially, in agingmice were caused by the low activity of the NADH-CoQ reductase that play important role in energyproduction needed for the hatching and trophoblast outgrowth.
Aktifitas dan pola makan, minum dan memamah biak kancil (Tragulus javanicus) di kebun binatang Ragunan Jakarta dan Surabaya Said, Nurhidayat; Winarto, Adi; Boediono, Arief; Djuwita, Ita; Nisa, Chairun; Wrediati, Tutik; Setijanto, Heru; Fakhrudin, Mohamad
Hemera Zoa Vol 77, No 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Telah dilakukan penelitian pada kancil (Tmgulus javanicus) di Kebun Binatang Ragunan - Jakarta dan Kebun Binatang Wonocolo - Surabaya untuk mendapatkan jenis makanan yang disukai, dan aktifitas makan, minum dan ruminasi dari kancil yang dikandangkan.Pada penelitian ini, disediakan 10 jenis makanan dan empat jenis diantaranya yaitu pisang, kacang panjang, kangkung dan pepaya lebih disukai. Aktifitas makan dilakukan pada siang dan malam hari, dan pada saat istirahat kancil melakukan aktifitas ruminasi. Perilaku makan dan ruminasi ini berbeda dengan perilakunya di habitas asalnya. Sedangkan aktifitas minum, jarang dilakukan, hal ini mungkin disebabkan oleh tingginya kadar air di dalam makanan yang disediakan. Aktifitas makan dari kancil yang dikandangkan telah berubah menjadi diurnal dan nokturnal.
PROLIFERASI DAN DIFERENSIASI SEL TULANG TIKUS DALAM MEDIUM KULTUR IN VITRO YANG MENGANDUNG EKSTRAK BATANG Cissus quadrangula Salisb. (SIPATAH-PATAH) Djuwita, Ita; Pratiwi, Irma Amalia; Winarto, Adi; Sabri, Mustafa
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.789 KB) | DOI: 10.21157/j.ked.hewan.v6i2.295

Abstract

Penelitian mengenai pengaruh ekstrak batang Cissus quadrangula Salisb. (sipatah-patah) terhadap tingkat proliferasi dan diferensiasi sel-sel tulang tikus (Sprague Dawley) prepuber umur empat minggu menggunakan sistem kultur in vitro. Sel-sel tulang dikultur dalam medium dulbecco’s modified eagle’s medium (DMEM) yang diberi tambahan newborn calf serum (NBCS) 10%, non essential amino acid (NEAA) 10%, NaHCO3, ITS 1 µl/ml (mengandung insulin 5 μg/ml, transferin 10 μg/ml, selenium 5 μg/ml; Sigma St Louis USA), dan 50 µg/ml gentamisin (mDMEM), dengan dan tanpa ekstrak Cissus quadrangula (CQ). Penelitian terdiri atas lima perlakuan yakni kontrol positif (mDMEM + deksametason 10-8 M), kontrol negatif (mDMEM), dan tiga konsentrasi CQ: mDMEM + CQ 0,3 mg/ml; mDMEM + CQ 0,6 mg/ml; dan mDMEM + CQ 1,2 mg/ml. Kultur dilakukan dalam inkubator CO2 5%, pada suhu 37° C selama tujuh hari. Parameter yang diamati adalah konsentrasi sel, komposisi, diameter osteoblas, dan diameter osteosit. Konsentrasi sel dihitung menggunakan hemositometer Newbauer. Komposisi osteoblas dan osteosit ditentukan berdasarkan pengamatan morfologi di bawah mikroskop cahaya. Diameter sel diukur menggunakan eyepiece micrometer. Data dianalisis menggunakan analisis varians dan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak Cissus quadrangula Salisb. pada konsentrasi 0,3 mg/ml; 0,6 mg/ml; dan 1,2 mg/ml secara signifikan dapat meningkatkan proliferasi sel tulang; dan pada konsentrasi 0,6 mg/ml mampu menginduksi diferensiasi osteoblas menjadi osteosit (P<0,05). Disimpulkan bahwa pemberian ekstrak Cissus quadrangula pada konsentrasi 0,6 mg/ml ke dalam medium kultur dapat meningkatkan proliferasi dan diferensiasi osteoblas.
Effect of Intravenous Polyunsaturated Fatty Acids Administration on Gastric Mucosal Integrity in Pig-tailed Macaques with Obstructive Jaundice Makmun, Dadang; Sajuthi, Dondin; Daldiyono, Daldiyono; Winarto, Adi; Sulistiawati, Erni
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 1, April 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/12120118-14

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Background: Acute gastric mucosal injury commonly occurs in patients with obstructive jaundice. We studied the effect of intravenous polyunsaturated fatty acids (PUFA) administration on gastric mucosal integrity in pig-tailed macaques (Macaca nemestrina) with obstructive jaundice by ligating common bile duct (CBD). Method: The study was conducted between February 2009 and May 2010 at the Primate Research Center, Bogor Agricultural Institute. Eight selected male pig-tailed macaques with 6.625 ± 0.83 kg of body weight were used and divided into two groups. In both groups, laboratory examination, including liver function tests and upper gastrointestinal endoscopy were performed before CBD ligation and every two weeks after ligation. In the first group, intravenous PUFA with the dose of 2 g/day was administered every day since four weeks post-ligation up to four weeks later, and in the second group, intravenous PUFA was administered since before ligation up to eight weeks later. Results: In both groups, increased total bilirubin, direct bilirubin, indirect bilirubin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and gamma glutamyl transpeptidase were obviously found, meanwhile decreased albumin level was detected and the cholinesterase level of both groups remained unchanged. Ulcer formation occurred among the first group during 4 weeks after CBD ligation, and these ulcers showed obvious healing within four weeks after intravenous PUFA administration. In the second group, there was no significant ulcer formation within eight weeks after CBD ligation. Conclusion: The potential appearance of acute gastric mucosal injury which reflected by ulcer formation in pig-tailed macaques with obstructive jaundice was significantly decreased by intravenous PUFA administration. We also have successfully developed animal model of obstructive jaundice by CBD ligation, based on the result of liver function tests. Keywords: acute gastric mucosal injury, intravenous PUFA administration, obstructive jaundice, pig- tailed macaques