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Pemaduan Pseudomonas putida 27.4B dan Trichorderma sp. dalam Media Cair Senyawa Humik yang Diberi Zeolit untuk Mendapatkan Produk Multifungsi Ramah Lingkungan WINARSO, Sugeng; HANDAYANTO, Eko; ., SYEKHFANI; SULISTYANTO, Didik
Jurnal Pengendalian Hayati Vol 1, No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Phosphate solubilizing bacteria (Pseudomonas putida 27.4B) is effective in dissolving undissolve P;humik compound can decrease Al exchange in acid soil and can be used as microorganism liquidmedia; Zeolite can improve CEC and bases concentration; and Trichorderma sp. can control diseaseand increase crop yield. Therefore, aim of this research is to make product having multifunction bothenvironment friendly soil amandement (fertilizer) and biopesticide, combination Pseudomonas putida27.4B, humic compound, zeolite, and Trichorderma sp.Potetoes Dextro Agar (PDA) media used to see resistance and growth, continued humic compoundliquid media added zeolite. Research design is completele randomized design with three factor are: 1)Trichorderma sp. concentration are: 0 (control); 104 CFU.ml-1; 105 CFU.ml-1; dan 106 CFU.ml-1; 2)good combination obtained last research are: a). 1000 ppm humic compound; 0,1% zeolit; P. putida27.4B 1012 CFU.ml-1 and b). 5000 ppm humic compound; 0,1% zeolit; P. putida 27.4B 1012 CFU.ml-1; and 3) incubation time are: 2 and 4 weeks.Result of research indicate that combination Pseudomonas putida 27.4B and Trichorderma sp. inPDA media can grow together, with visually speed of Trichorderma sp. growth faster thanPseudomonas putida 27.4B. Pseudomonas putida 27.4B proven can live on, even very good, in humiccompound liquid media added zeolite and Trichorderma sp. Its best growth is at humic compoundconcentration 1000 ppm. Besides that is tendency of combination Pseudomonas putida 27.4B andTrichorderma sp. exactly can increase growth of Pseudomonas putida 27.4B both at humic compoundconcentration 1000 ppm and without addition of humic compound. While at humic compoundconcentration 5000 ppm, Trichorderma sp. inokulasi exactly decrease Pseudomonas putida 27.4B.
Viabilitas Nematoda Entomopatogen Steinernema spp. dalam Media Kombinasi Senyawa Humik WAGIYANA, -; SULISTYANTO, Didik; WINARSO, Sugeng
Jurnal Pengendalian Hayati Vol 1, No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Its well known, That Entomopathogenic Nematods (EPNs) used as biological agentswhich effective to control pest of Spodoptera litura (Noctuidae: Lepidoptera).The aims of thisresearch were to find out the multifungsion product as well as a biofeltilizer and biopesticide whichsuitable for agriculture sustainability, secondly the materials were to mixed the humic compound andbiological agent Entomopathogenic Nematodes (EPNs). Combination of biopesticide with EPNs asactive ingredient and humic compound as biofertilizer to find out a good product combination whichindicated the highest viability and activity of EPNs Steinernema spp on humic compound, in orderapplication of these meterial more effective and efficient to prepare the soil fertility and pest problem.This research was done with inoculation of EPNs on the medium/culture e.c: phosphate solublebacteria Pseudomonas putida 27.4B, zeolit and the humic compound (liquid culture). Pathogenecitytest of EPNs were done after incubation on the humic compound to the larvae of S. litura.The result of this research showed that long time of viability EPNs only for fourth weeks on the humicculture at (4000 ppm). Actually the EPNs from this incubation could 100 % mortality of the S. lituralarvae after 72 hours inoculation. The infection rate of EPNs on the larvae S. litura was 30,3 tailsduring 24 hours incubation. The viability of EPNs Steinernema spp on the medium with 1000 ppmhumic compound was 20 % after 120 hours and after 4 weeks later all EPNs could not survive on thismedium. It seems, the medium containing zeolith, P. putida 27.4B and humic compound at 1000 ppmwas not suitable medium neither for nematods growth or larvae S. litura survival.
Effectiveness of Foliar Application of Potassium on Flowering and Fruiting of Cocoa Erwiyono, Rudy; Sucahyo, Achmad; ., Suyono; Winarso, Sugeng
Pelita Perkebunan (Coffee and Cocoa Research Journal) Vol 22, No 1 (2006)
Publisher : Indonesian Coffee and Cocoa Research Institute

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Abstract

An experiment of foliar application of potassium on cocoa has been carried out in Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute in Jember to study its effectiveness on the flowering and fruiting of cocoa. The experiment was arranged according to factorial design of 2 factors with environmental design of randomized complete blocks. The first factor was soil application of potassium fertilization in two levels, i.e. without fertilizer and with K-fertilizer as KCl. The second factor was application of potassium from two sources, i.e. KCl and KNO 3 , with 3 levels, i.e. without fertilizer (sprayed with water only), K-fertilizer as KCl and K-fertilizer as KNO 3. The experiment was done in 4 replicates. Parameters observed included newly formed flowers, new young fruits and the fresh-developing fruits. The results showed that foliar application of fertilizer was more effective than soil application and plant response was better in increasing new flower formation. On the other hand, the effect of soil application of fertilizer was slow. Foliar application of potassium followed by soil application of potassium fertilizer was more effective in the form of KNO 3 rather than in the form of KCl in increasing new flower formation. The dosage of foliar application of potassium could not significantly increase new young fruit formation. On the other hand, the dosage of soil application of potassium could increase new young fruit formation significantly. Key words: foliar application, kalium, flower, young fruit, cocoa.
SINTESIS HIDROGEL KOPOLIMER PATI ILES-ILES DENGAN ASAM AKRILAT, AKRILAMIDA DAN METILENABISA KRILAMIDA SEBAGAI PEMBENAH TANAH Sjaifullah, Achmad; Winarso, Sugeng; Santoso, Agung Budi
Jurnal Sains Materi Indonesia Vol 17, No 1: OKTOBER 2015
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.324 KB) | DOI: 10.17146/jsmi.2015.17.1.4201

Abstract

SINTESIS HIDROGEL KOPOLIMER PATI ILES-ILES DENGAN ASAM AKRILAT, AKRILAMIDA DAN METILENABISA KRILAMIDA SEBAGAI PEMBENAH TANAH.Hidrogel kopolimer cangkok pati iles-iles dengan asam akrilat, akrilamida dan metilena bis-akrilamida disintesis menggunakan teknik polimerisasi dalam larutan dengan pelarut air dan inisiator kaliumpersulfat. Pati iles-iles yang digunakan diperoleh dengan menghomogenkan umbi iles-iles, mencucinya dengan air dan menggumpalkannya dengan metanol beberapa kali.Kelarutan pati iles-iles yang diperoleh dengan cara tersebutmencapai 5%b/b. Polimerisasi dalam larutan dilakukan tanpa hembusan gas nitrogen. Hidrogel yang dihasilkan ditentukan daya serap airnya dan struktur kimianya berdasarkan spektrumFTIR. Berdasarkan berat hidrogel yang dihasilkan dapat dikatakan bahwa sintesis menghasilkan 100 % polimerisasi pada suhu 55-60 °C. Hidrogel yang dihasilkan pada kondisi polimerisasi tertentu mempunyai daya serap air hingga 51 kali, yaitu yang disintesis dengan perbandingan konsentrasi pati iles-iles dengan asamakrilat, akrilamida dan bis-akrilamida = 1:3:3:0,01. Hidrogel kopolimer kemudian digunakan sebagai pembenah tanah dengan mempelajari kemampuan tanah berpasir dalammenyimpan dan menguapkan airnya. Tanah berpasir yang dicampur dengan 0,5 % b/b hidrogel dapat menyerap air hingga dua setengah kali dari pada tanah tanpa hidrogel dan dapat menahan lebih dari separo air yang diserapnya saat terjadi kesetimbangan penguapan dan penyerapan air. Kesetimbangan penguapan dan penyerapan air dicapai setelah 5 hari pada suhu udara 23-29 °C dan kelembaban 40-83 %.
PENAMBAHAN NUTRISI PADA TIGA VARIETAS MELON UNTUK MENINGKATKAN HASIL DAN KUALITAS BUAH [NUTRITION IN ADDITION TO IMPROVE THE TREE VARIETIES OF MELON AND FRUIT QUALITY] Deus, Ananias De; Hariyono, Kacung; Winarso, Sugeng
AGRITROP Vol 12, No 2 (2014): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.135 KB) | DOI: 10.32528/agr.v12i2.720

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Tujuan penelitian adalah untuk mengetahui interaksi antara penambahan nutrisi dan tiga varietas terhadap produksi dan kualitas buah melon dan untuk menentukan pengaruh penambahan nutrisi atau mengetahui respons varietas yang berbeda terhadap produksi dan kualitas buah melon. Penelitian ini dilakukan di lahan UPT Agrotekno Park, Universitas Jember, Indonesia mulai dari April sampai Juni 2014. Penelitian ini dirancang dengan Rancangan Acak Kelompok Faktorial 3x3, dengan tiga ulangan. Faktor pertama, penambahan nutrisi yang terdiri dari 0 g NPK + ZA 0 g, 1,5 g NPK + ZA 1,5 g, dan 3 g NPK + ZA 3 g. Faktor kedua, varietas melon terdiri dari varietas Glamour, Aromis dan Action 434. Data eksperimen dianalisis menggunakan analisis varians dan dilanjutkan dengan Duncan Multiple Range Test (DMRT) jika ada efek yang signifikan. Hasil penelitian menunjukkan bahwa interaksi antara gizi dan varietas berpengaruh nyata terhadap kadar gula buah dan vitamin C. Penambahan nutrisi berpengaruh terhadap pertumbuhan dan kualitas buah melon, dosis 3 g pupuk NPK + 3 g ZA menunjukkan dosis terbaik. Varietas berpengaruh nyata terhadap pertumbuhan dan kualitas buah melon dan varietas Glamour sebagai varietas terbaik. Kata kunci: Melon, penambahan nutrisi, varietas dan kualitas buah
EFEKTIVITAS PUPUK ORGANIK DIPERKAYA PADA PERTUMBUHAN VEGETATIF TABULAMPOT JAMBU AIR MADU DELI HIJAU (Syzygium samarangense (blume) Merr. & L.M. Perry). Zuhro, Fatimatuz; Hasanah, Hasni Ummul; Winarso, Sugeng; Habibah, Siti
AGRITROP Vol 16, No 2 (2018): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v16i2.1811

Abstract

Pupuk organik diperkaya memiliki kandungan unsur hara yang lengkap, sehingga cocok diaplikasikan pada budidaya tabulampot jambu air Madu Deli Hijau (MDH). Penelitian ini bertujuan untuk mengetahui efektivitas pupuk organik diperkaya terhadap pertumbuhan vegetatif  tabulampot jambu air MDH. Penelitian ini dilakukan di lahan percobaan mulai bulan Maret sampai Juli 2018, menggunakan rancangan acak kelompok  dengan 3 perlakuan, yaitu: kontrol negatif (tanpa pupuk), kontrol positif (penambahan Urea dan NPK), dan penambahan pupuk organik dosis 3 kg/pot. Hasil penelitian menunjukkan bahwa aplikasi pupuk organik diperkaya berpengaruh nyata terhadap parameter pertambahan jumlah daun dan jumlah cabang primer, tetapi tidak berpengaruh nyata pada pertambahan tinggi dan jumlah cabang sekunder. Hasil uji Duncan menunjukkan bahwa pemberian pupuk organik diperkaya memiliki pengaruh yang sama dengan kontrol positif, sehingga aplikasi pupuk organik diperkaya pada tabulampot jambu air MDH dianggap  efektif dan mampu menggantikan aplikasi pupuk anorganik.
Pengaruh Kombinasi Senyawa Humik dan CaCO3 terhadap Alumunium dan Fosfat Typic Paleudult Kentrong Banten Winarso, Sugeng; Handayanto, Eko; Syekhfani, Syekhfani; Sulistyanto, Didik
Journal of Tropical Soils Vol 14, No 2: May 2009
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2009.v14i2.%p

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Effect of Humic Compounds and CaCO3 Combination on Aluminium and Phosphate of Typic Paleudult Kentrong Banten (S. Winarso, E. Handayanto, Syekhfani and D. Sulistyanto): Decomposition of organic matter releases humic compounds, that can chelate metals include Al in acidic soils. This chelation of Al is important for decreasing of Al activity and P fixation in acidic soils. This study was aimed to test the effect of humic compounds extracted from rice-straw compost and CaCO3 combination on aluminium activity and phosphate (available P) on an Ultisol (Typic Paleudult) collected from Kentrong Banten.  A laboratory study was conducted by series consentration of CaCO3 (0, 500, 1.000, 2.000, dan 3.000 ppm) in 8 grams of acidic soil. The acidic soil had previously been subjected to continuously addition of humic compounds and none. Then, these mix of soil and CaCO3 were added 40 ml contained 5.000 ppm humic compounds to obtain 1:5 soil:suspension ratio and  control was made by using aquadest. They were shaked for 2 hours every day and pH was measured. At 11st days incubation  Alexch (N KCl) and soluble of P (PB and PC) were measured.  The results  showed that liming or addition of CaCO3 to the acidic soils decreased Alexch (precipitated to be Al(OH)3) linearly with formula y = -0.778x + 6.108; y = Alexch (Cmol kg-1), x = CaCO3 (M); R² = 0,916. Combination addition of humic compounds and CaCO3 was able to increase pH and Alexch up to not detected. The increased of P-soluble or desorption P untill 384% took place at addition of 0.0016 M CaCO3 and 5000 ppm humic compounds. More desorption of P was observed if the acidic soils has previously been subjected to continuously addition of humic compounds, such us the increased of soluble P up to 739% at addition of CaCO3 0.0008 M.
Aluminum Exchangeable and Phosphorous Availability on Ultisol Using Humic Substance and Synthetic Organic Acid Winarso, Sugeng; Taufiq, Abdullah
Journal of Tropical Soils Vol 16, No 3: September 2011
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2011.v16i3.%p

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Humic substance (HS) extracted from composted agricultural waste contains organic acids that potential for an acid soil amandement. Functional group of COOH and OH in synthetically organic acid is higher than in HS originated from composted agricultural waste. Addition of synthetically organic acid to the HS may increase its functional group and therefore it will increase effectiveness in detoxifying aluminum (Al) and desorpting phosphorus (P) from an acid soil. Objective of reseach was to determine effect of synthetic organic acid addition to HS extracted from composted organic matter in desorpting P and detoxify Al. Research was conducted at Soil Laboratory of Agricultural Faculty of Jember University from February to May 2010. Treatment consisted of combination of four levels of HS concentration extracted from composted rice straw (0; 1,000; 2,000; and 5,000 mg kg-1) with two kind of synthetic organic acid: ethylenediaminetetraacetic acid (EDTA) and acetic acid (CH3COOH) at concentration of 5 mM. The treatment laid out in a randomized complete by design with three replications. Ultisol collected from Kentrong Banten was used in this reseach. Observation consisted of pH, Al and P concentration in the suspension at 0, 1, 2, 3, and 4 weeks after incubation. The result showed that EDTA or acetic acid treatment at concentration of 5 mM effectively increased soil pH and decreased exchangeable Al (exch-Al). However, EDTA was more reactive and having higher capability in increasing pH and detoxifying exch-Al than acetic acid. Humic substance extracted from composted rice straw at concentration of 1,000 to 5,000 mg kg-1 also effectively increased soil pH, decreased exch-Al, and increased P availability during a 4 week incubation period. The HS effect in Al detoxification and P desorption in acid soil could be boosted by addition of EDTA or acetic acid synthetic
Aluminum Detoxification by Humic Substance Extracted from Compost of Organic Wastes Winarso, Sugeng; Handayanto, Eko; Taufiq, Abdullah
Journal of Tropical Soils Vol 15, No 1: January 2010
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2010.v15i1.%p

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Aluminum Detoxification by Humic Substance Extracted from Compost of Organic Wastes (S Winarso, E Handayanto and A Taufiq):  Humic substance could control the activity of metal as well as exchangeable aluminum (Alexch) on acid soil. The humic substance extracted from compost of agricultural waste is rarely conducted. The objective of research was to evaluate the effect of humic substance concentration and sources to pH and Al suspension. The research had been conducted in the Soil Laboratory of Agricultural Faculty of Jember University from April to July 2009. The humic substance used in these researches was extracted from compost of rice straw (RS), soybean straw (SS), cassava starch solid waste (CSSW), and empty bunch of oil palm tree (EBOPT). The humic substance concentration treatments were 0, 10, 20, 30, 40, 50, 75, 100, 150, 200, 250, and 300 ppm. Result of the analysis showed that characteristic of humic substance was difference depending on the source material used. The humic substance extracted from SS contain total base cations (K, Na, Ca, Mg) higher (1.17%) compared with CSSW (1.07%), OPT  (0.87%), and RS (0.69%). While the humic substances extracted from RS contain total organic acid (Acetic, Fumeric, cetoglutamic, Sucsinic, Propionic, Butiric, Ocsalic, and sitric acid) higher (157 ppm) compared with EBOPT (129 ppm), SS (115%), and CSSW (108%). Among these organic acids, acetic acid was highest concentration (> 34.51% of total acid). The addition of humic substance increased pH and decreased aluminum concentration of the suspension. The higher pH increment and Al concentration reduction took placed in the suspension treated with humic substance from SS compost. The correlation between decreasing Al concentration with humic substance concentration which explained chelation was higher (r = 0.97) than decreasing Al concentration with increasing pH which explained precipitation (r = 0.93). Based on these research results, it can be concluded that humic substance extracted from agricultural waste have a good prospect as a soil ameliorant to increase soil pH, detoxify soil aluminum, and increase soil P availability on acid soil.  
PENGARUH WAKTU PEMBERIAN PUPUK KANDANG TERHADAP PERTUMBUHAN VEGETATIF TABULAMPOT JAMBU AIR MDH (Syzygium samarangense (Blume) Merr. & L.M. Perry) Afidah, Yusfi; Zuhro, Fatimatuz; Hasanah, Hasni Ummul; Winarso, Sugeng; Hoesain, Mohammad
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

MDH water apple is one type of fruit plant that can be cultivated intabulampot. Facts in the field indicate that planting it in tabulampotmakes the plant bear fruit faster. Another factor that determines theproduction of MDH guava is fertilization, including fertilizing withorganic matter. Besides giving organic fertilizer, the right time whenapplying fertilizer is also important in determining the growth of MDHguava. The purpose of this study was to determine the effect of the timeof manure on the vegetative growth of MDH guava. This research wasconducted in the green house in March to August 2018. This study useda randomized block design with 9 repetitions and 3 treatments, namely:(1) fertilization at the beginning of planting, (2) fertilization in the 1stand 3rd month after planting, and (3) fertilization in the 1st and 5thmonths after planting. Data were analyzed using ANOVA test. Theresults of this study indicate that manure has a significant effect on thenumber of leaf parameters, but does not significantly affect plantheight, number of primary branches, and number of secondarybranches. The best growth results were obtained at two time offertilizing in MDH water apple. Keyword : MDH water apple, manure, tabulampot, time of fertilizing.